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The map label for this gene is yifB [H]

Identifier: 88608871

GI number: 88608871

Start: 61185

End: 62690

Strand: Direct

Name: yifB [H]

Synonym: NSE_0068

Alternate gene names: 88608871

Gene position: 61185-62690 (Clockwise)

Preceding gene: 88608037

Following gene: 88608534

Centisome position: 7.12

GC content: 41.63

Gene sequence:

>1506_bases
TTGTTTACGGAGATTCATACAATTTCAATTCAGGGGCTTAGTCTAGTCAAAATAGGAGTTCAAGTTGCTATATCACCTGG
ACTGCCATGTTTTAACATTGTTGGTTTGCCTGATAAATCAATCAGTGAGTCTAAGGAAAGAATAAGGGCGGCACTCCTCC
ATATAGGTTTGTCCTTACCTAAAAAGAGAATAACAGTTAATCTTTCCCCAGCAGGTTTGACAAAAATAGGGACACACTAT
GATCTTCCAATAGCGCTTGGTGTAATTCAGTTGTTGGATAGTAATTTTGTTAACAGAAATTTTTCAGAGTTTGTCGTACT
TGGTGAATTGTCCCTAGATGCTTCGGTAAAGGGTGTTGCGGGAGTCCTTCCAGCGGCTATTTATGCTAAAGAACGCAATC
TGTGGCTAGTTTGTCCATGGGAGAATAGGATAGAAGCACGTATTTCTAAGAATAAGAAGCTTCTACCGATCGCCTCACTA
AATGAATTGACATCATTTTTAATGGGGAAATCTACACCCAAAAAGATTTGCGATACCTTTGTGCTGGAGGAAGATTCCTA
TCCTGATATGAAGGATGTTAAAGGGCAAAAAATTGCAAAGCGGGCAATCGAAATAGCTGCCGCAGGTGGACATCACATGC
TTATGCGTGGACCTCCCGGTACTGGTAAATCGATGCTTGCGAAGAGAATCGTCGGCATCATGCCACCCCTTTTGCCCAAG
GAGATCATGGAAGTGAATACGATTATCAGTGTCTCTGGGGAGTTTACAAATAAGATTTATAGAAAACGTCCCTTTCGAGA
GCCACATCAATCAGCTTCAGCTGCAGCAATGATCGGTGGTGGTGCAGTACCAAAACCAGGTGAGGTGACACTTGCACACA
ATGGAGTACTTTTTCTGGATGAAATGGCTGAGTTTTCTAGTCATGTATTAGATACACTCAGACAGGTGCTGGAAACAGGT
GAGGTAACAATATCTCGTGCAAATATGAAATTGACTTACCCTGCGTCTTTTCAGTTGATAGGCGCTATGAATCCCTGTAG
ATGTGGCTATTTGGGCTATCTTCCGGAAAAGGAGTGTAAAAATTCGAAGAAATGCGGACTGGAGTACATGAAAACGATTT
CAGGTCCTTTTCTCGATAGGATAGATATCTTTGTGAATATGGAACCGATAAACAAGAATCAGTTTTTTGAATCGCAGGAT
GGAGAATCGTCTGCTTTAATCAGAAAGAGAGTTATTGCCGCGCGAAACAAGCAATATGAAAGGTACAATAAAGTGAAGTT
CAGTTTGAACTGTCACATGGACAATCACGCGCTTGCCGAACATGTTTCACTTGATAATGAAACAAGGGAGTTCCTAAAGG
CTACTTTTGAGAAAGGTTATATTTCCCTACGTGGTCACGACCGTATACTGAAGGTGGCACGCACTATAGCAGACCTTGAT
GGTTCGGATGAGGTGAAAAAAGCTCATCTAACTGAGGCGCTTTACTATAGGATGAGTCAAAATTAA

Upstream 100 bases:

>100_bases
TCTATTTCAAGAACCTACAAGCCTAAACAACAGAATCGGATTATGATAAAGGAAACTCTAACATAAATAAGTAAATATTA
CCTTCTGGTAGGTACGATTT

Downstream 100 bases:

>100_bases
GAGAATAGTTGCGTTTATATGTAGACTCATATTAGCGTACGCACACAACCTTATAGCTCAGTCTATGGACCGCACCTTAC
TTTTTGGCTGTGGAGTTATT

Product: Mg chelatase-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MFTEIHTISIQGLSLVKIGVQVAISPGLPCFNIVGLPDKSISESKERIRAALLHIGLSLPKKRITVNLSPAGLTKIGTHY
DLPIALGVIQLLDSNFVNRNFSEFVVLGELSLDASVKGVAGVLPAAIYAKERNLWLVCPWENRIEARISKNKKLLPIASL
NELTSFLMGKSTPKKICDTFVLEEDSYPDMKDVKGQKIAKRAIEIAAAGGHHMLMRGPPGTGKSMLAKRIVGIMPPLLPK
EIMEVNTIISVSGEFTNKIYRKRPFREPHQSASAAAMIGGGAVPKPGEVTLAHNGVLFLDEMAEFSSHVLDTLRQVLETG
EVTISRANMKLTYPASFQLIGAMNPCRCGYLGYLPEKECKNSKKCGLEYMKTISGPFLDRIDIFVNMEPINKNQFFESQD
GESSALIRKRVIAARNKQYERYNKVKFSLNCHMDNHALAEHVSLDNETREFLKATFEKGYISLRGHDRILKVARTIADLD
GSDEVKKAHLTEALYYRMSQN

Sequences:

>Translated_501_residues
MFTEIHTISIQGLSLVKIGVQVAISPGLPCFNIVGLPDKSISESKERIRAALLHIGLSLPKKRITVNLSPAGLTKIGTHY
DLPIALGVIQLLDSNFVNRNFSEFVVLGELSLDASVKGVAGVLPAAIYAKERNLWLVCPWENRIEARISKNKKLLPIASL
NELTSFLMGKSTPKKICDTFVLEEDSYPDMKDVKGQKIAKRAIEIAAAGGHHMLMRGPPGTGKSMLAKRIVGIMPPLLPK
EIMEVNTIISVSGEFTNKIYRKRPFREPHQSASAAAMIGGGAVPKPGEVTLAHNGVLFLDEMAEFSSHVLDTLRQVLETG
EVTISRANMKLTYPASFQLIGAMNPCRCGYLGYLPEKECKNSKKCGLEYMKTISGPFLDRIDIFVNMEPINKNQFFESQD
GESSALIRKRVIAARNKQYERYNKVKFSLNCHMDNHALAEHVSLDNETREFLKATFEKGYISLRGHDRILKVARTIADLD
GSDEVKKAHLTEALYYRMSQN
>Mature_501_residues
MFTEIHTISIQGLSLVKIGVQVAISPGLPCFNIVGLPDKSISESKERIRAALLHIGLSLPKKRITVNLSPAGLTKIGTHY
DLPIALGVIQLLDSNFVNRNFSEFVVLGELSLDASVKGVAGVLPAAIYAKERNLWLVCPWENRIEARISKNKKLLPIASL
NELTSFLMGKSTPKKICDTFVLEEDSYPDMKDVKGQKIAKRAIEIAAAGGHHMLMRGPPGTGKSMLAKRIVGIMPPLLPK
EIMEVNTIISVSGEFTNKIYRKRPFREPHQSASAAAMIGGGAVPKPGEVTLAHNGVLFLDEMAEFSSHVLDTLRQVLETG
EVTISRANMKLTYPASFQLIGAMNPCRCGYLGYLPEKECKNSKKCGLEYMKTISGPFLDRIDIFVNMEPINKNQFFESQD
GESSALIRKRVIAARNKQYERYNKVKFSLNCHMDNHALAEHVSLDNETREFLKATFEKGYISLRGHDRILKVARTIADLD
GSDEVKKAHLTEALYYRMSQN

Specific function: Unknown

COG id: COG0606

COG function: function code O; Predicted ATPase with chaperone activity

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Mg-chelatase subunits D/I family. ComM subfamily [H]

Homologues:

Organism=Escherichia coli, GI145693202, Length=501, Percent_Identity=40.9181636726547, Blast_Score=374, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004482
- InterPro:   IPR000523
- InterPro:   IPR020568 [H]

Pfam domain/function: PF01078 Mg_chelatase [H]

EC number: NA

Molecular weight: Translated: 55583; Mature: 55583

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTEIHTISIQGLSLVKIGVQVAISPGLPCFNIVGLPDKSISESKERIRAALLHIGLSLP
CCCEEEEEEECCCEEEEEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCC
KKRITVNLSPAGLTKIGTHYDLPIALGVIQLLDSNFVNRNFSEFVVLGELSLDASVKGVA
CEEEEEEECCCCCHHCCCCCCCHHHHHHHHHHHCHHCCCCCCCEEEEEEECCCCCCCHHH
GVLPAAIYAKERNLWLVCPWENRIEARISKNKKLLPIASLNELTSFLMGKSTPKKICDTF
HHHHHHHEEECCCEEEEECCCCHHHHHHCCCCCEEEEHHHHHHHHHHHCCCCHHHHHHHH
VLEEDSYPDMKDVKGQKIAKRAIEIAAAGGHHMLMRGPPGTGKSMLAKRIVGIMPPLLPK
EECCCCCCCHHHCCHHHHHHHHHHHEECCCCEEEEECCCCCCHHHHHHHHHHHCCCCCHH
EIMEVNTIISVSGEFTNKIYRKRPFREPHQSASAAAMIGGGAVPKPGEVTLAHNGVLFLD
HHHHHHHEEEECCHHHHHHHHCCCCCCCHHCCCCEEEECCCCCCCCCCEEEEECCEEEHH
EMAEFSSHVLDTLRQVLETGEVTISRANMKLTYPASFQLIGAMNPCRCGYLGYLPEKECK
HHHHHHHHHHHHHHHHHHCCCEEEEECCEEEECCCCEEEEECCCCCCCCCCCCCCCHHHC
NSKKCGLEYMKTISGPFLDRIDIFVNMEPINKNQFFESQDGESSALIRKRVIAARNKQYE
CCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHH
RYNKVKFSLNCHMDNHALAEHVSLDNETREFLKATFEKGYISLRGHDRILKVARTIADLD
HHCEEEEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHCCC
GSDEVKKAHLTEALYYRMSQN
CCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFTEIHTISIQGLSLVKIGVQVAISPGLPCFNIVGLPDKSISESKERIRAALLHIGLSLP
CCCEEEEEEECCCEEEEEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCC
KKRITVNLSPAGLTKIGTHYDLPIALGVIQLLDSNFVNRNFSEFVVLGELSLDASVKGVA
CEEEEEEECCCCCHHCCCCCCCHHHHHHHHHHHCHHCCCCCCCEEEEEEECCCCCCCHHH
GVLPAAIYAKERNLWLVCPWENRIEARISKNKKLLPIASLNELTSFLMGKSTPKKICDTF
HHHHHHHEEECCCEEEEECCCCHHHHHHCCCCCEEEEHHHHHHHHHHHCCCCHHHHHHHH
VLEEDSYPDMKDVKGQKIAKRAIEIAAAGGHHMLMRGPPGTGKSMLAKRIVGIMPPLLPK
EECCCCCCCHHHCCHHHHHHHHHHHEECCCCEEEEECCCCCCHHHHHHHHHHHCCCCCHH
EIMEVNTIISVSGEFTNKIYRKRPFREPHQSASAAAMIGGGAVPKPGEVTLAHNGVLFLD
HHHHHHHEEEECCHHHHHHHHCCCCCCCHHCCCCEEEECCCCCCCCCCEEEEECCEEEHH
EMAEFSSHVLDTLRQVLETGEVTISRANMKLTYPASFQLIGAMNPCRCGYLGYLPEKECK
HHHHHHHHHHHHHHHHHHCCCEEEEECCEEEECCCCEEEEECCCCCCCCCCCCCCCHHHC
NSKKCGLEYMKTISGPFLDRIDIFVNMEPINKNQFFESQDGESSALIRKRVIAARNKQYE
CCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHH
RYNKVKFSLNCHMDNHALAEHVSLDNETREFLKATFEKGYISLRGHDRILKVARTIADLD
HHCEEEEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHCCC
GSDEVKKAHLTEALYYRMSQN
CCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1995430; 1379743; 9278503 [H]