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The map label for this gene is pyrB

Identifier: 88608852

GI number: 88608852

Start: 430123

End: 431001

Strand: Direct

Name: pyrB

Synonym: NSE_0510

Alternate gene names: 88608852

Gene position: 430123-431001 (Clockwise)

Preceding gene: 88608519

Following gene: 88608092

Centisome position: 50.07

GC content: 39.7

Gene sequence:

>879_bases
ATGAGAAGTCTACTGAGTAATAAGGATATTACGGTTGATGAAATTTATGAGATCTTCGATCTGGCTGCTTTCTATCTAAA
GAATGGAATTAAGAATGACCTGCTTGGAAAAGTGGTTTTCTCGGTTTTTTTTGAGAGCTCGACGCGCACCGAAACTTCTT
TTTCGCTTGCTGCTAGCAAGCTCGGTTGCCTAGTTGAAAAAGTGAATGTGTCAACTGTCGCACTAAAAAAGGGTGAGCAC
TTGGTGGATTTTTTTACTTCCTTAAATGCTCTTTCACCGGATGCATTCATCATACGATACAGCCAAAGTGGTGTTCTTCA
CATTTTAGAAAAATATATTGATTGTTGCATTATTAATGCAGGTGATGGGACAAACGAACATCCAACACAGGGATTGATAG
ATGCATTTACCATACTCCTACTCAAGGAAAAGATAGAGGGTTTAAATATACTTGTCTGCGGAGATATCATAAGGAGTAGG
GTTGCTCACTCTACCATACCTATACTTCAAAGACTTGGAGCAACTGTTGCAATTTCTGGGCCGTTAAGTTTTATGCCCAC
AATCCAAGTAGAAGAGATTGTTTACTATGATAACTTTCCTGCAGCGGCTAAATCTGCTGATGTGATAATAATGCTGCGTA
CCCAGACCGAACGTATGGGACAAAAAGTTTTAGCTGGTGCTACGGCTAATTTTCATCTTGATGAAAATATTCTGAAAGAC
TTAGGTACCGACGTGCTCATAATGCATCCAGGACCCATTCATAGAAATTGTGAGATTTCCAATGCTGTCCTTGATGACTC
CAGGAGTACAGTACTTACACAAGTAAGGAATTCCCAATATGTGAGGGCAGCTGTATTGAACTTTCTCATGAAAAGTTAG

Upstream 100 bases:

>100_bases
TTGTTGATGTTTCTAGCATCCTTTGTGTTGACACTGCTATTATGGTATTTTAACGCTCGTGCAACTTTGAACTTAAAGAA
AATAGAGAAGCTGCTTTTGT

Downstream 100 bases:

>100_bases
CACCTACTGCTATCAAGAAAATAAATTTGGAATAGGGTAGACGCCCCTATGGGGTAGTATGACAAGTTCTTTGTAACAAT
TAAGTCTAGCTGATGTCATG

Product: aspartate carbamoyltransferase

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MRSLLSNKDITVDEIYEIFDLAAFYLKNGIKNDLLGKVVFSVFFESSTRTETSFSLAASKLGCLVEKVNVSTVALKKGEH
LVDFFTSLNALSPDAFIIRYSQSGVLHILEKYIDCCIINAGDGTNEHPTQGLIDAFTILLLKEKIEGLNILVCGDIIRSR
VAHSTIPILQRLGATVAISGPLSFMPTIQVEEIVYYDNFPAAAKSADVIIMLRTQTERMGQKVLAGATANFHLDENILKD
LGTDVLIMHPGPIHRNCEISNAVLDDSRSTVLTQVRNSQYVRAAVLNFLMKS

Sequences:

>Translated_292_residues
MRSLLSNKDITVDEIYEIFDLAAFYLKNGIKNDLLGKVVFSVFFESSTRTETSFSLAASKLGCLVEKVNVSTVALKKGEH
LVDFFTSLNALSPDAFIIRYSQSGVLHILEKYIDCCIINAGDGTNEHPTQGLIDAFTILLLKEKIEGLNILVCGDIIRSR
VAHSTIPILQRLGATVAISGPLSFMPTIQVEEIVYYDNFPAAAKSADVIIMLRTQTERMGQKVLAGATANFHLDENILKD
LGTDVLIMHPGPIHRNCEISNAVLDDSRSTVLTQVRNSQYVRAAVLNFLMKS
>Mature_292_residues
MRSLLSNKDITVDEIYEIFDLAAFYLKNGIKNDLLGKVVFSVFFESSTRTETSFSLAASKLGCLVEKVNVSTVALKKGEH
LVDFFTSLNALSPDAFIIRYSQSGVLHILEKYIDCCIINAGDGTNEHPTQGLIDAFTILLLKEKIEGLNILVCGDIIRSR
VAHSTIPILQRLGATVAISGPLSFMPTIQVEEIVYYDNFPAAAKSADVIIMLRTQTERMGQKVLAGATANFHLDENILKD
LGTDVLIMHPGPIHRNCEISNAVLDDSRSTVLTQVRNSQYVRAAVLNFLMKS

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=302, Percent_Identity=31.1258278145695, Blast_Score=136, Evalue=2e-32,
Organism=Escherichia coli, GI2367364, Length=301, Percent_Identity=27.906976744186, Blast_Score=111, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI193204318, Length=320, Percent_Identity=27.8125, Blast_Score=107, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6322331, Length=303, Percent_Identity=30.6930693069307, Blast_Score=122, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24642586, Length=288, Percent_Identity=29.5138888888889, Blast_Score=109, Evalue=2e-24,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 32170; Mature: 32170

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSLLSNKDITVDEIYEIFDLAAFYLKNGIKNDLLGKVVFSVFFESSTRTETSFSLAASK
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
LGCLVEKVNVSTVALKKGEHLVDFFTSLNALSPDAFIIRYSQSGVLHILEKYIDCCIINA
HHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHEEEEEC
GDGTNEHPTQGLIDAFTILLLKEKIEGLNILVCGDIIRSRVAHSTIPILQRLGATVAISG
CCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHCCCEEEECC
PLSFMPTIQVEEIVYYDNFPAAAKSADVIIMLRTQTERMGQKVLAGATANFHLDENILKD
CCCCCCCEEEEEEEEECCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCCCEEECHHHHHH
LGTDVLIMHPGPIHRNCEISNAVLDDSRSTVLTQVRNSQYVRAAVLNFLMKS
CCCCEEEECCCCCCCCCCCCHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRSLLSNKDITVDEIYEIFDLAAFYLKNGIKNDLLGKVVFSVFFESSTRTETSFSLAASK
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
LGCLVEKVNVSTVALKKGEHLVDFFTSLNALSPDAFIIRYSQSGVLHILEKYIDCCIINA
HHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHEEEEEC
GDGTNEHPTQGLIDAFTILLLKEKIEGLNILVCGDIIRSRVAHSTIPILQRLGATVAISG
CCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHCCCEEEECC
PLSFMPTIQVEEIVYYDNFPAAAKSADVIIMLRTQTERMGQKVLAGATANFHLDENILKD
CCCCCCCEEEEEEEEECCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCCCEEECHHHHHH
LGTDVLIMHPGPIHRNCEISNAVLDDSRSTVLTQVRNSQYVRAAVLNFLMKS
CCCCEEEECCCCCCCCCCCCHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA