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The map label for this gene is leuS

Identifier: 88608827

GI number: 88608827

Start: 425402

End: 427957

Strand: Reverse

Name: leuS

Synonym: NSE_0506

Alternate gene names: 88608827

Gene position: 427957-425402 (Counterclockwise)

Preceding gene: 88608081

Following gene: 88608123

Centisome position: 49.82

GC content: 39.12

Gene sequence:

>2556_bases
GTGTCATGCAACTTGTCTTGTGCTAGAATCCTGTTCGGGCTGTTTACAGTTGGAATACACATGTCGAATTACGATTTTAA
GAAAATAGAGCAATTTTCTCAATCATTGTGGGATTTTTCCAGTTGTGACTTCCAGAGAAAAAAGTACTATGTTCTGAGTA
TGTTTCCCTACCCGTCTGGAAAACTACATATTGGACATCTGAGAAACTATGTTATTGGAGACGTCTTAGCTCGTTATAAG
AGGAGCCAGGGATACAATGTCTTACACCCTATTGGTTGGGATGCGTTTGGTCTACCCGCTGAAAAAGCAGCAATAAATAG
CAATATTCACCCAAGGATATGGACAGAAAAAAATATCGCAAAGATGCGAACCTCTCTCAAGAGCATAGGTCTGTCGTATG
ATTGGAGCAGAGAACTTTCAACCTGTTCTCCCAAATATTATGAGCACGAACAAAAATTTTTTTTGGCCTTTCTAAAAGCT
GGTTTAGCATACAGAAAAAATTCCGAAGTAAATTGGGATCCTATCGACAAAACAGTACTTGCAAACGAACAGGTCATTGA
TGGGCGTGGTTGGAGATCGGGTGCGGTTGTCGTGAAGAGAAAGATTCCGCAGTGGTTCCTTAAAATTAGCGACTTTTCTG
AAGAACTCCTGAAGGGATTAGAGTCACTTACTGGCTGGCCAGAGAAGGTTAAAACTATGCAAACAAATTGGATAGGAAAA
TCTGAAGGGGCAGTGATAAAATTTTCTATAAGCGGATATGAGGAAAGATTTATTGAGGTATTCACTACTAGACCCGAGAC
GATCTTTGGAGCGTCATTTTGTGCTATTTCAGTGAATCACCCTTTGGTTAATGAATTAAATTTATTGAGTAATGCAGAGA
GAGAAATACTGCGCATACAAACTACCTGTGAGTCAGATCATTACGATGACAACAAAAACTTTTCTGGGACGACAAATCTT
GAGAAAGTTCATGAGCAAAAGCTTGGCATTCTAACAAAAATAAAAGTGAAGCATCCCTTTTCCGGCGATGAGCTTCCTTT
ATACGTAGCAAATTTTGTATTTGCAGAATACGGTTCGGGGGCAATATTCGGTTGCCCAGCTCATGATACACGAGATTTTG
CATTCGCACAGCAATACAATTTACCGTGTTTAAGAGTCATCTCAGCAAAATGCACAAATACTGCACTACCCTACTGTTTG
GAAGAAGGAGTAATGTGTAATTCAAATTTCCTAGATGGAATGCAGGTGATAGAGGCACGAAAATTTATAATTGAAAAAAT
TTCTTCGATTGGTATTGGTGAGGCAAAGAACTCATATAGATTGCGAGACTGGGGAATATCAAGGCAGCGATATTGGGGCT
GTCCAATTCCGGTTGTGTACTGTGAAAAGTGTTCGATGCAACCGGTAAAAGTTGAAGATCTACCTGTCACCCTTCCCGAA
GAAGTAGAGTTTACAGTACAGGGTAATCCACTAGAACACCATCCAACATGGAAATACACAAATTGTCCAAAATGTGGCGG
TCCTGCAGTTAGGGACACAGATACTTTTGATACATTTTTCGAATCTTCTTGGTATTTTGCGGCTTTTTGCAGTAAAGAAG
GAGGAATAGTTAAGGAATCTTGCAAGCGTTTTCTTCCTGTCGATATGTACATCGGCGGAATAGAACATGCAATATTGCAT
TTACTTTATGCTAGGTTCTTTACGCGCGCCTTAAACAAATGTGGATACATCGATATAGTGGAACCTTTTCGTAATCTACT
CACACAAGGTATGGTTTGTCATGAGACTTACCAGAACTCTTCCGGTGAATATTTGTATCCTGAAGAAGCAAAATCACTGC
TGGAAAAAGGTGAAAAAGTTCTGGTTGGCAAAATCGAGAAAATGAGTAAATCCAAAAAAAATGTTGTAGATCTTGAAGAA
ATCGTCTCAAAATATGGCGCTGATGCAGCAAGGTTCTTTATTCTTTCTGATAATCCTCCAGAAAACAGTTTTGGATGGTC
AGACAGGGGCATAAATGCGTCGTTTAAGTTTTTGCAAAGAATAAAAAATCTTGTAGAAAGATATCTTTCCAGTAAAAACA
GTGGTGAGGTTCAAACAGGCCAGAACGTTAAAATCCAGATTAACAAGATTATCTGCGATATGACGAAGTACATGGACGAA
ATAAAATTAAATTGCGCAGTTGCAAAGATTCACGAGTTGGTCAACTTGCTGAGCGCGTCCGGAGCATTATCTAAAGAAAC
GATTCACGTACTTTTAAGGATACTCGAGCCATTTGCACCACACTTAGCTGAATATTTAGCTTCAAAGCTTGAAAACAATG
TCATTCTATATGGTTCCCCGTGGGTTGCTTATAACGAAGTACTCACTCAAAGCGATGCGGTCACATTAATGGTGCGGAAG
AATGGAAAATTCGTACAATTGTTAGAAGTAAAAAAGGATTCTTCTGAAGAAGAGATAATTTCCCAAGCAAAAAGATTGAT
CAAGAATGCCGTAGTTGATAAAGTTATATATGTTCCTGGTAAGATGGTTAATTTCCTTTGCAGCAGTCCTGGTTAA

Upstream 100 bases:

>100_bases
CCTCCTGTTTCACCAGAAAATGTTGCAGATATTACTTAAATTTGGATATAGGAAAACGCTGCAAAGTAGAAAAAAAGAAA
GTTGATACACATGTATTATT

Downstream 100 bases:

>100_bases
CCGAATGTTTAGAACGTTAAATTTAAGATACTTTGTTGGTATATTCTGCTGTGCGGAATTACAGTTGGGTTTCACTATGG
TGGTTTATTAGTTATGGCTT

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 851; Mature: 850

Protein sequence:

>851_residues
MSCNLSCARILFGLFTVGIHMSNYDFKKIEQFSQSLWDFSSCDFQRKKYYVLSMFPYPSGKLHIGHLRNYVIGDVLARYK
RSQGYNVLHPIGWDAFGLPAEKAAINSNIHPRIWTEKNIAKMRTSLKSIGLSYDWSRELSTCSPKYYEHEQKFFLAFLKA
GLAYRKNSEVNWDPIDKTVLANEQVIDGRGWRSGAVVVKRKIPQWFLKISDFSEELLKGLESLTGWPEKVKTMQTNWIGK
SEGAVIKFSISGYEERFIEVFTTRPETIFGASFCAISVNHPLVNELNLLSNAEREILRIQTTCESDHYDDNKNFSGTTNL
EKVHEQKLGILTKIKVKHPFSGDELPLYVANFVFAEYGSGAIFGCPAHDTRDFAFAQQYNLPCLRVISAKCTNTALPYCL
EEGVMCNSNFLDGMQVIEARKFIIEKISSIGIGEAKNSYRLRDWGISRQRYWGCPIPVVYCEKCSMQPVKVEDLPVTLPE
EVEFTVQGNPLEHHPTWKYTNCPKCGGPAVRDTDTFDTFFESSWYFAAFCSKEGGIVKESCKRFLPVDMYIGGIEHAILH
LLYARFFTRALNKCGYIDIVEPFRNLLTQGMVCHETYQNSSGEYLYPEEAKSLLEKGEKVLVGKIEKMSKSKKNVVDLEE
IVSKYGADAARFFILSDNPPENSFGWSDRGINASFKFLQRIKNLVERYLSSKNSGEVQTGQNVKIQINKIICDMTKYMDE
IKLNCAVAKIHELVNLLSASGALSKETIHVLLRILEPFAPHLAEYLASKLENNVILYGSPWVAYNEVLTQSDAVTLMVRK
NGKFVQLLEVKKDSSEEEIISQAKRLIKNAVVDKVIYVPGKMVNFLCSSPG

Sequences:

>Translated_851_residues
MSCNLSCARILFGLFTVGIHMSNYDFKKIEQFSQSLWDFSSCDFQRKKYYVLSMFPYPSGKLHIGHLRNYVIGDVLARYK
RSQGYNVLHPIGWDAFGLPAEKAAINSNIHPRIWTEKNIAKMRTSLKSIGLSYDWSRELSTCSPKYYEHEQKFFLAFLKA
GLAYRKNSEVNWDPIDKTVLANEQVIDGRGWRSGAVVVKRKIPQWFLKISDFSEELLKGLESLTGWPEKVKTMQTNWIGK
SEGAVIKFSISGYEERFIEVFTTRPETIFGASFCAISVNHPLVNELNLLSNAEREILRIQTTCESDHYDDNKNFSGTTNL
EKVHEQKLGILTKIKVKHPFSGDELPLYVANFVFAEYGSGAIFGCPAHDTRDFAFAQQYNLPCLRVISAKCTNTALPYCL
EEGVMCNSNFLDGMQVIEARKFIIEKISSIGIGEAKNSYRLRDWGISRQRYWGCPIPVVYCEKCSMQPVKVEDLPVTLPE
EVEFTVQGNPLEHHPTWKYTNCPKCGGPAVRDTDTFDTFFESSWYFAAFCSKEGGIVKESCKRFLPVDMYIGGIEHAILH
LLYARFFTRALNKCGYIDIVEPFRNLLTQGMVCHETYQNSSGEYLYPEEAKSLLEKGEKVLVGKIEKMSKSKKNVVDLEE
IVSKYGADAARFFILSDNPPENSFGWSDRGINASFKFLQRIKNLVERYLSSKNSGEVQTGQNVKIQINKIICDMTKYMDE
IKLNCAVAKIHELVNLLSASGALSKETIHVLLRILEPFAPHLAEYLASKLENNVILYGSPWVAYNEVLTQSDAVTLMVRK
NGKFVQLLEVKKDSSEEEIISQAKRLIKNAVVDKVIYVPGKMVNFLCSSPG
>Mature_850_residues
SCNLSCARILFGLFTVGIHMSNYDFKKIEQFSQSLWDFSSCDFQRKKYYVLSMFPYPSGKLHIGHLRNYVIGDVLARYKR
SQGYNVLHPIGWDAFGLPAEKAAINSNIHPRIWTEKNIAKMRTSLKSIGLSYDWSRELSTCSPKYYEHEQKFFLAFLKAG
LAYRKNSEVNWDPIDKTVLANEQVIDGRGWRSGAVVVKRKIPQWFLKISDFSEELLKGLESLTGWPEKVKTMQTNWIGKS
EGAVIKFSISGYEERFIEVFTTRPETIFGASFCAISVNHPLVNELNLLSNAEREILRIQTTCESDHYDDNKNFSGTTNLE
KVHEQKLGILTKIKVKHPFSGDELPLYVANFVFAEYGSGAIFGCPAHDTRDFAFAQQYNLPCLRVISAKCTNTALPYCLE
EGVMCNSNFLDGMQVIEARKFIIEKISSIGIGEAKNSYRLRDWGISRQRYWGCPIPVVYCEKCSMQPVKVEDLPVTLPEE
VEFTVQGNPLEHHPTWKYTNCPKCGGPAVRDTDTFDTFFESSWYFAAFCSKEGGIVKESCKRFLPVDMYIGGIEHAILHL
LYARFFTRALNKCGYIDIVEPFRNLLTQGMVCHETYQNSSGEYLYPEEAKSLLEKGEKVLVGKIEKMSKSKKNVVDLEEI
VSKYGADAARFFILSDNPPENSFGWSDRGINASFKFLQRIKNLVERYLSSKNSGEVQTGQNVKIQINKIICDMTKYMDEI
KLNCAVAKIHELVNLLSASGALSKETIHVLLRILEPFAPHLAEYLASKLENNVILYGSPWVAYNEVLTQSDAVTLMVRKN
GKFVQLLEVKKDSSEEEIISQAKRLIKNAVVDKVIYVPGKMVNFLCSSPG

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=864, Percent_Identity=34.375, Blast_Score=470, Evalue=1e-132,
Organism=Homo sapiens, GI46852147, Length=697, Percent_Identity=22.8120516499283, Blast_Score=91, Evalue=5e-18,
Organism=Escherichia coli, GI1786861, Length=872, Percent_Identity=39.6788990825688, Blast_Score=652, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71997517, Length=243, Percent_Identity=44.0329218106996, Blast_Score=218, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI71997510, Length=243, Percent_Identity=44.0329218106996, Blast_Score=218, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI212645227, Length=292, Percent_Identity=29.4520547945205, Blast_Score=131, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71980946, Length=149, Percent_Identity=27.5167785234899, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323414, Length=854, Percent_Identity=37.2365339578454, Blast_Score=536, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6321531, Length=408, Percent_Identity=24.2647058823529, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6325217, Length=455, Percent_Identity=21.978021978022, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21355409, Length=764, Percent_Identity=34.2931937172775, Blast_Score=421, Evalue=1e-117,
Organism=Drosophila melanogaster, GI281366294, Length=728, Percent_Identity=21.2912087912088, Blast_Score=96, Evalue=1e-19,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 96693; Mature: 96562

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCNLSCARILFGLFTVGIHMSNYDFKKIEQFSQSLWDFSSCDFQRKKYYVLSMFPYPSG
CCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCC
KLHIGHLRNYVIGDVLARYKRSQGYNVLHPIGWDAFGLPAEKAAINSNIHPRIWTEKNIA
CEEHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHCCCCCCCEECCHHHH
KMRTSLKSIGLSYDWSRELSTCSPKYYEHEQKFFLAFLKAGLAYRKNSEVNWDPIDKTVL
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
ANEQVIDGRGWRSGAVVVKRKIPQWFLKISDFSEELLKGLESLTGWPEKVKTMQTNWIGK
CCCEEECCCCCCCCCEEEEHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
SEGAVIKFSISGYEERFIEVFTTRPETIFGASFCAISVNHPLVNELNLLSNAEREILRIQ
CCCCEEEEEECCHHHHHHHHHCCCCCCEECCEEEEEECCCCCHHHHHHHHCCCHHHEEEE
TTCESDHYDDNKNFSGTTNLEKVHEQKLGILTKIKVKHPFSGDELPLYVANFVFAEYGSG
ECCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCC
AIFGCPAHDTRDFAFAQQYNLPCLRVISAKCTNTALPYCLEEGVMCNSNFLDGMQVIEAR
CEEECCCCCCCCHHHHHHCCCCHHHHHHHHHCCCCCCHHHHCCCEECCCCHHHHHHHHHH
KFIIEKISSIGIGEAKNSYRLRDWGISRQRYWGCPIPVVYCEKCSMQPVKVEDLPVTLPE
HHHHHHHHHCCCCCCCCCCEEHHCCCCCCCCCCCCCCHHHHCCCCCCCEEECCCCCCCCC
EVEFTVQGNPLEHHPTWKYTNCPKCGGPAVRDTDTFDTFFESSWYFAAFCSKEGGIVKES
CEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCCCCHHHH
CKRFLPVDMYIGGIEHAILHLLYARFFTRALNKCGYIDIVEPFRNLLTQGMVCHETYQNS
HHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCCEEHHHHCCC
SGEYLYPEEAKSLLEKGEKVLVGKIEKMSKSKKNVVDLEEIVSKYGADAARFFILSDNPP
CCCEECHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEEEECCCCC
ENSFGWSDRGINASFKFLQRIKNLVERYLSSKNSGEVQTGQNVKIQINKIICDMTKYMDE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCEEEEHHHHHHHHHHHHHH
IKLNCAVAKIHELVNLLSASGALSKETIHVLLRILEPFAPHLAEYLASKLENNVILYGSP
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
WVAYNEVLTQSDAVTLMVRKNGKFVQLLEVKKDSSEEEIISQAKRLIKNAVVDKVIYVPG
CHHHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEECCH
KMVNFLCSSPG
HHHHHHHCCCC
>Mature Secondary Structure 
SCNLSCARILFGLFTVGIHMSNYDFKKIEQFSQSLWDFSSCDFQRKKYYVLSMFPYPSG
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCC
KLHIGHLRNYVIGDVLARYKRSQGYNVLHPIGWDAFGLPAEKAAINSNIHPRIWTEKNIA
CEEHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHCCCCCCCEECCHHHH
KMRTSLKSIGLSYDWSRELSTCSPKYYEHEQKFFLAFLKAGLAYRKNSEVNWDPIDKTVL
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
ANEQVIDGRGWRSGAVVVKRKIPQWFLKISDFSEELLKGLESLTGWPEKVKTMQTNWIGK
CCCEEECCCCCCCCCEEEEHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
SEGAVIKFSISGYEERFIEVFTTRPETIFGASFCAISVNHPLVNELNLLSNAEREILRIQ
CCCCEEEEEECCHHHHHHHHHCCCCCCEECCEEEEEECCCCCHHHHHHHHCCCHHHEEEE
TTCESDHYDDNKNFSGTTNLEKVHEQKLGILTKIKVKHPFSGDELPLYVANFVFAEYGSG
ECCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCC
AIFGCPAHDTRDFAFAQQYNLPCLRVISAKCTNTALPYCLEEGVMCNSNFLDGMQVIEAR
CEEECCCCCCCCHHHHHHCCCCHHHHHHHHHCCCCCCHHHHCCCEECCCCHHHHHHHHHH
KFIIEKISSIGIGEAKNSYRLRDWGISRQRYWGCPIPVVYCEKCSMQPVKVEDLPVTLPE
HHHHHHHHHCCCCCCCCCCEEHHCCCCCCCCCCCCCCHHHHCCCCCCCEEECCCCCCCCC
EVEFTVQGNPLEHHPTWKYTNCPKCGGPAVRDTDTFDTFFESSWYFAAFCSKEGGIVKES
CEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCCCCHHHH
CKRFLPVDMYIGGIEHAILHLLYARFFTRALNKCGYIDIVEPFRNLLTQGMVCHETYQNS
HHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCCEEHHHHCCC
SGEYLYPEEAKSLLEKGEKVLVGKIEKMSKSKKNVVDLEEIVSKYGADAARFFILSDNPP
CCCEECHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEEEECCCCC
ENSFGWSDRGINASFKFLQRIKNLVERYLSSKNSGEVQTGQNVKIQINKIICDMTKYMDE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCEEEEHHHHHHHHHHHHHH
IKLNCAVAKIHELVNLLSASGALSKETIHVLLRILEPFAPHLAEYLASKLENNVILYGSP
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
WVAYNEVLTQSDAVTLMVRKNGKFVQLLEVKKDSSEEEIISQAKRLIKNAVVDKVIYVPG
CHHHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEECCH
KMVNFLCSSPG
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA