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The map label for this gene is pgk

Identifier: 88608817

GI number: 88608817

Start: 355523

End: 356650

Strand: Direct

Name: pgk

Synonym: NSE_0436

Alternate gene names: 88608817

Gene position: 355523-356650 (Clockwise)

Preceding gene: 88608445

Following gene: 88608822

Centisome position: 41.39

GC content: 39.18

Gene sequence:

>1128_bases
ATGGTTGACGTAGAAGATGTAAAAGACTGCAGAGTAGTTCTTCGGGTCGATTTTAATGTTCCGGTTAATCAAGAGGGTTT
AATAACTGACTTTTCCAGGATTGCGGTAGTTTTACCAACTATAAATCTACTTTTAAAAAATGGTTGCGCTATATTGTTGC
TCTCTCATTTTGGTAAACCAACTGCTGCTATCAAGGAGAAATTCTCATTTGAGAGAATCATTGGACAGATTTGTCAGTTC
ACTGGCTTAGATATAAGGGTTGTGTCAGATCTGAGCAATGAACTTCTACTCAGACAAGGGGAGATTATTCTTTTAGAAAA
CGTGAGATTCTTTGCTGGCGAGGTGGAGAACGATTCTAAATTTGCAATGCTTATTGCAAGACACGGTGATGTGTATGTAA
ATGATGCTTTTTCTGTTTCGCACAGGGAACATGCATCCGTAGTAGGAATACCACAATTTCTTCCATCTGCTATGGGTTTG
GCATTTTTACGGGAATATCAAGAGCTAGATAGTGCAGCTACGAAGGGTACTGTTGCAGTAATCGGTGGCAGTAAAATTTC
GACGAAGCTGCCATTAATACGAGGTCTTCTAAAAAAAGTAAAAATGGTCATATTGGGTGGTGCTCTGGTAAATGTGATAT
TGCGTTCTAGAGGTTATAACGTGGGCAAATCCATGTGTGAGGATTGTCACTGTGATATTACATTACCTGAACTTTTTATA
CCATTTGATTTCGTTACCTCACATGGGGTTGAGGATCAAAATTTCCACTTTACCGATGCCAGGATGATTCCTCACAATGA
AATGATTTTGGATATAGGGCCAGCATCGCTCATTGCAATGAAGGGCATTCTTTCAAAAGCAGAGAAAATTTTGTGGAATG
GTCCAATTGGTGCATTTGAATTTGAACCGTTTTGTAGTGGTACACATGAGTTAGCTAGATTTATTTCTTTTCTAACAAAG
AATGCGAACATTAAGAGCATAATAGGAGGAGGCGACACCTTAGCCGCAATTAAATCACTCCAGGAGCCATGTTTTTCCTA
TATCTCTACATCCGGTGGTGCTTTTCTTAAATTTCAGGAGGAGGGCACACTCCCTGGAATAGAAGCCATAAAAAAATGCA
AAGTATAA

Upstream 100 bases:

>100_bases
CTTCTTCCTTTCAAAAGGGATATTTCAGTTGTATTTAGCAGCACAGAAATGACATGACTGTCTACGTAAAATGTAATGAA
TTTTTCAATTTTATCGATAA

Downstream 100 bases:

>100_bases
TCTTGATAAGTTATTGATAGCAGATATGTGTGAGAAGCATCAAATTGTATATGATGGCGAAGGTGGAAGCACTATAAATC
AGATAGGAATTGGGGTTCTA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MVDVEDVKDCRVVLRVDFNVPVNQEGLITDFSRIAVVLPTINLLLKNGCAILLLSHFGKPTAAIKEKFSFERIIGQICQF
TGLDIRVVSDLSNELLLRQGEIILLENVRFFAGEVENDSKFAMLIARHGDVYVNDAFSVSHREHASVVGIPQFLPSAMGL
AFLREYQELDSAATKGTVAVIGGSKISTKLPLIRGLLKKVKMVILGGALVNVILRSRGYNVGKSMCEDCHCDITLPELFI
PFDFVTSHGVEDQNFHFTDARMIPHNEMILDIGPASLIAMKGILSKAEKILWNGPIGAFEFEPFCSGTHELARFISFLTK
NANIKSIIGGGDTLAAIKSLQEPCFSYISTSGGAFLKFQEEGTLPGIEAIKKCKV

Sequences:

>Translated_375_residues
MVDVEDVKDCRVVLRVDFNVPVNQEGLITDFSRIAVVLPTINLLLKNGCAILLLSHFGKPTAAIKEKFSFERIIGQICQF
TGLDIRVVSDLSNELLLRQGEIILLENVRFFAGEVENDSKFAMLIARHGDVYVNDAFSVSHREHASVVGIPQFLPSAMGL
AFLREYQELDSAATKGTVAVIGGSKISTKLPLIRGLLKKVKMVILGGALVNVILRSRGYNVGKSMCEDCHCDITLPELFI
PFDFVTSHGVEDQNFHFTDARMIPHNEMILDIGPASLIAMKGILSKAEKILWNGPIGAFEFEPFCSGTHELARFISFLTK
NANIKSIIGGGDTLAAIKSLQEPCFSYISTSGGAFLKFQEEGTLPGIEAIKKCKV
>Mature_375_residues
MVDVEDVKDCRVVLRVDFNVPVNQEGLITDFSRIAVVLPTINLLLKNGCAILLLSHFGKPTAAIKEKFSFERIIGQICQF
TGLDIRVVSDLSNELLLRQGEIILLENVRFFAGEVENDSKFAMLIARHGDVYVNDAFSVSHREHASVVGIPQFLPSAMGL
AFLREYQELDSAATKGTVAVIGGSKISTKLPLIRGLLKKVKMVILGGALVNVILRSRGYNVGKSMCEDCHCDITLPELFI
PFDFVTSHGVEDQNFHFTDARMIPHNEMILDIGPASLIAMKGILSKAEKILWNGPIGAFEFEPFCSGTHELARFISFLTK
NANIKSIIGGGDTLAAIKSLQEPCFSYISTSGGAFLKFQEEGTLPGIEAIKKCKV

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=411, Percent_Identity=32.360097323601, Blast_Score=186, Evalue=4e-47,
Organism=Homo sapiens, GI31543397, Length=410, Percent_Identity=32.6829268292683, Blast_Score=176, Evalue=3e-44,
Organism=Escherichia coli, GI1789294, Length=381, Percent_Identity=37.5328083989501, Blast_Score=213, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17508823, Length=404, Percent_Identity=32.9207920792079, Blast_Score=194, Evalue=7e-50,
Organism=Saccharomyces cerevisiae, GI6319857, Length=402, Percent_Identity=32.8358208955224, Blast_Score=176, Evalue=6e-45,
Organism=Drosophila melanogaster, GI17136394, Length=405, Percent_Identity=34.0740740740741, Blast_Score=190, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24581203, Length=405, Percent_Identity=32.5925925925926, Blast_Score=186, Evalue=2e-47,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 41106; Mature: 41106

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDVEDVKDCRVVLRVDFNVPVNQEGLITDFSRIAVVLPTINLLLKNGCAILLLSHFGKP
CCCHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHEEEEHHCCCC
TAAIKEKFSFERIIGQICQFTGLDIRVVSDLSNELLLRQGEIILLENVRFFAGEVENDSK
HHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCEEEEECCEEEECCCCCCCE
FAMLIARHGDVYVNDAFSVSHREHASVVGIPQFLPSAMGLAFLREYQELDSAATKGTVAV
EEEEEEECCCEEEECCEECCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
IGGSKISTKLPLIRGLLKKVKMVILGGALVNVILRSRGYNVGKSMCEDCHCDITLPELFI
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEECHHHHC
PFDFVTSHGVEDQNFHFTDARMIPHNEMILDIGPASLIAMKGILSKAEKILWNGPIGAFE
CHHHHHHCCCCCCCEEEECCEECCCCCEEEEECCHHHHHHHHHHHHHHHHEECCCCCCEE
FEPFCSGTHELARFISFLTKNANIKSIIGGGDTLAAIKSLQEPCFSYISTSGGAFLKFQE
CCHHCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
EGTLPGIEAIKKCKV
CCCCCHHHHHHHCCC
>Mature Secondary Structure
MVDVEDVKDCRVVLRVDFNVPVNQEGLITDFSRIAVVLPTINLLLKNGCAILLLSHFGKP
CCCHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHEEEEHHCCCC
TAAIKEKFSFERIIGQICQFTGLDIRVVSDLSNELLLRQGEIILLENVRFFAGEVENDSK
HHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCEEEEECCEEEECCCCCCCE
FAMLIARHGDVYVNDAFSVSHREHASVVGIPQFLPSAMGLAFLREYQELDSAATKGTVAV
EEEEEEECCCEEEECCEECCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
IGGSKISTKLPLIRGLLKKVKMVILGGALVNVILRSRGYNVGKSMCEDCHCDITLPELFI
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEECHHHHC
PFDFVTSHGVEDQNFHFTDARMIPHNEMILDIGPASLIAMKGILSKAEKILWNGPIGAFE
CHHHHHHCCCCCCCEEEECCEECCCCCEEEEECCHHHHHHHHHHHHHHHHEECCCCCCEE
FEPFCSGTHELARFISFLTKNANIKSIIGGGDTLAAIKSLQEPCFSYISTSGGAFLKFQE
CCHHCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
EGTLPGIEAIKKCKV
CCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA