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The map label for this gene is rho

Identifier: 88608814

GI number: 88608814

Start: 59313

End: 60632

Strand: Reverse

Name: rho

Synonym: NSE_0067

Alternate gene names: 88608814

Gene position: 60632-59313 (Counterclockwise)

Preceding gene: 88608868

Following gene: 88608292

Centisome position: 7.06

GC content: 42.95

Gene sequence:

>1320_bases
ATGACTAGTGATCCTGAAAAGGTTATTAACTTGTCTGACTTGCGTTCAAAAAGTGCACGAGAGCTTTTAACAATGGCAGA
GTCATTAAATTTGCAGAATCTAGCTGGGATGTCAAAGCAGGATCTGATAATCCTCATAGCACAGAGCGAAGTCGCAGACA
GTCAAACCAAGAAGGTTATAATGAGTTCCGGCGTAGCTGAGGTCCTGAACGAAGGTTTTGCCTTCATGCGATCCTCTGTA
TATAACTATCTTGCGAACCAAGATGACATTTATATCTCACCAGGACAGGTACGAAGACTCGGAATTAGGACAGGTGACCT
AATCTATGGCGAAGTAAGAGCTCCAAAAAAAGGCGAAAGGTATTTTGCACTTTCTAAGGCATTGGAAATCAATAACAGTC
CTGCGTCTGTTGCCAGAAGGTATGTTGCCTTCGAGAGTTTGGTTCCTCTTTATCCAGATACTCCAATAAAGCTTGAATGT
GAAAAGTCCACACGGCCTGTGGAGTCAAAAAACTGTGACTTAAGCGGTAGGATTATTGATATCGTTGCTCCGCTCGGAAG
AGGACAAAGAGCGCTTATAGTTGCACAACCAAAAACTGGTAAGACTATTCTCCTGCAAAACATCGCGCACTCTATTTCTG
CGAACTATCCGGATATACACTTGATAATCCTCCTCATTGATGAGCGACCAGAAGAGGTCACCGATATGCGCAGGTCAGTA
AATGCAGAGGTCGTGAGCTCGACATTCGACGAACCAGCGGACAAACACGTATACGTTGCCGAATTTGCAATTGAAAAAGC
AAAAAGACTGGTCGAACAGGGAAAACACGTCGTTATTTTACTTGATTCGATTACTCGTTTGGCCAGGGCATACAATTCTG
TTGTTCCGTCTTCTGGCAAACTTTTGAGTGGCGGTGTTGATTCCAATGCCCTTCAAAAAGCAAAGCGTCTCTTCGGAGCG
GCACGCAATATTGAGGGAGGTGGGTCCTTGACTATTATTGGAACTGCGCTTGTAGATACTGGCTCAAGGATGGACGAACT
TATTTACGAAGAGTTTAAGGGTACTGGTAACTCCGAAATAATTCTTGATAGGAAGCTTGCAGAAAAGCGTCTGTTTCCAG
CGATTGATATAACTAAGTCAGGCACAAGAAAAGAAGAACTCCTTCTAGAACGCTCTATCCTGAACAAAGTTTGGGTCTTG
AGACGGATTCTTAACCAGATGACGGATGCAGATGCAATCGAATTCATAATAGACAAGCTGCAAAGAACGAAAAATAACGA
AGAGTTTTTCCTCTTTATGGATAAAAAAGTTAACAACTGA

Upstream 100 bases:

>100_bases
GATAAAAAGAAATACTTTGAAGCTTGGAAAAAGAGCTGAACCTTGCAAAGTTGAGGAAACGAAGAATCCTGTGATAGCAG
AAACACGATGTGAGCACGCC

Downstream 100 bases:

>100_bases
AGAAGCTCGCGCTGTTAGGTTAGATCTTCAACAAAGAGACGTCTAACAGGAGCTTTGTGTGTTTTTACTGATGTCTGCTA
GGGAAATGGAGGATGGATGC

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MTSDPEKVINLSDLRSKSARELLTMAESLNLQNLAGMSKQDLIILIAQSEVADSQTKKVIMSSGVAEVLNEGFAFMRSSV
YNYLANQDDIYISPGQVRRLGIRTGDLIYGEVRAPKKGERYFALSKALEINNSPASVARRYVAFESLVPLYPDTPIKLEC
EKSTRPVESKNCDLSGRIIDIVAPLGRGQRALIVAQPKTGKTILLQNIAHSISANYPDIHLIILLIDERPEEVTDMRRSV
NAEVVSSTFDEPADKHVYVAEFAIEKAKRLVEQGKHVVILLDSITRLARAYNSVVPSSGKLLSGGVDSNALQKAKRLFGA
ARNIEGGGSLTIIGTALVDTGSRMDELIYEEFKGTGNSEIILDRKLAEKRLFPAIDITKSGTRKEELLLERSILNKVWVL
RRILNQMTDADAIEFIIDKLQRTKNNEEFFLFMDKKVNN

Sequences:

>Translated_439_residues
MTSDPEKVINLSDLRSKSARELLTMAESLNLQNLAGMSKQDLIILIAQSEVADSQTKKVIMSSGVAEVLNEGFAFMRSSV
YNYLANQDDIYISPGQVRRLGIRTGDLIYGEVRAPKKGERYFALSKALEINNSPASVARRYVAFESLVPLYPDTPIKLEC
EKSTRPVESKNCDLSGRIIDIVAPLGRGQRALIVAQPKTGKTILLQNIAHSISANYPDIHLIILLIDERPEEVTDMRRSV
NAEVVSSTFDEPADKHVYVAEFAIEKAKRLVEQGKHVVILLDSITRLARAYNSVVPSSGKLLSGGVDSNALQKAKRLFGA
ARNIEGGGSLTIIGTALVDTGSRMDELIYEEFKGTGNSEIILDRKLAEKRLFPAIDITKSGTRKEELLLERSILNKVWVL
RRILNQMTDADAIEFIIDKLQRTKNNEEFFLFMDKKVNN
>Mature_438_residues
TSDPEKVINLSDLRSKSARELLTMAESLNLQNLAGMSKQDLIILIAQSEVADSQTKKVIMSSGVAEVLNEGFAFMRSSVY
NYLANQDDIYISPGQVRRLGIRTGDLIYGEVRAPKKGERYFALSKALEINNSPASVARRYVAFESLVPLYPDTPIKLECE
KSTRPVESKNCDLSGRIIDIVAPLGRGQRALIVAQPKTGKTILLQNIAHSISANYPDIHLIILLIDERPEEVTDMRRSVN
AEVVSSTFDEPADKHVYVAEFAIEKAKRLVEQGKHVVILLDSITRLARAYNSVVPSSGKLLSGGVDSNALQKAKRLFGAA
RNIEGGGSLTIIGTALVDTGSRMDELIYEEFKGTGNSEIILDRKLAEKRLFPAIDITKSGTRKEELLLERSILNKVWVLR
RILNQMTDADAIEFIIDKLQRTKNNEEFFLFMDKKVNN

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=427, Percent_Identity=57.3770491803279, Blast_Score=500, Evalue=1e-143,
Organism=Escherichia coli, GI1788251, Length=248, Percent_Identity=26.6129032258064, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 48826; Mature: 48695

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSDPEKVINLSDLRSKSARELLTMAESLNLQNLAGMSKQDLIILIAQSEVADSQTKKVI
CCCCHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCEEEEEECCCCCCHHHHHHH
MSSGVAEVLNEGFAFMRSSVYNYLANQDDIYISPGQVRRLGIRTGDLIYGEVRAPKKGER
HHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEECCCCCEEEEECCCCCCCCC
YFALSKALEINNSPASVARRYVAFESLVPLYPDTPIKLECEKSTRPVESKNCDLSGRIID
EEEEHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEE
IVAPLGRGQRALIVAQPKTGKTILLQNIAHSISANYPDIHLIILLIDERPEEVTDMRRSV
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHC
NAEVVSSTFDEPADKHVYVAEFAIEKAKRLVEQGKHVVILLDSITRLARAYNSVVPSSGK
CHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHCCCCCCC
LLSGGVDSNALQKAKRLFGAARNIEGGGSLTIIGTALVDTGSRMDELIYEEFKGTGNSEI
EEECCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCEE
ILDRKLAEKRLFPAIDITKSGTRKEELLLERSILNKVWVLRRILNQMTDADAIEFIIDKL
EEEHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
QRTKNNEEFFLFMDKKVNN
HHCCCCCEEEEEECCCCCC
>Mature Secondary Structure 
TSDPEKVINLSDLRSKSARELLTMAESLNLQNLAGMSKQDLIILIAQSEVADSQTKKVI
CCCHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCEEEEEECCCCCCHHHHHHH
MSSGVAEVLNEGFAFMRSSVYNYLANQDDIYISPGQVRRLGIRTGDLIYGEVRAPKKGER
HHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEECCCCCEEEEECCCCCCCCC
YFALSKALEINNSPASVARRYVAFESLVPLYPDTPIKLECEKSTRPVESKNCDLSGRIID
EEEEHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEE
IVAPLGRGQRALIVAQPKTGKTILLQNIAHSISANYPDIHLIILLIDERPEEVTDMRRSV
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHC
NAEVVSSTFDEPADKHVYVAEFAIEKAKRLVEQGKHVVILLDSITRLARAYNSVVPSSGK
CHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHCCCCCCC
LLSGGVDSNALQKAKRLFGAARNIEGGGSLTIIGTALVDTGSRMDELIYEEFKGTGNSEI
EEECCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCEE
ILDRKLAEKRLFPAIDITKSGTRKEELLLERSILNKVWVLRRILNQMTDADAIEFIIDKL
EEEHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
QRTKNNEEFFLFMDKKVNN
HHCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]