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The map label for this gene is purF

Identifier: 88608791

GI number: 88608791

Start: 164808

End: 166199

Strand: Reverse

Name: purF

Synonym: NSE_0194

Alternate gene names: 88608791

Gene position: 166199-164808 (Counterclockwise)

Preceding gene: 88608592

Following gene: 88608748

Centisome position: 19.35

GC content: 46.05

Gene sequence:

>1392_bases
ATGGTGTCGCGGGATCAGTCTGGGTGGCTCACGGAGAAGTGTGGTGTTGTAGCTGTCGTTGGCACACCCAATGCTGTCGA
GCTTTCTCTCTTGGGGCTCCATGGACTACAGCATAGAGGACACGAGGCCTTTGGTATCGCATTTGTGCATAATGGTGACG
TGGGTGTCGTTCACCGCTTTGGCAGGATTATGTCGGTCAATCCAGTTGACCTTTCTATCCCCTCTGCTGAGACGGTAATT
GGACATGTGAGATACTCGACGAGTGGGGGCTCTGACTTTTCCCAGCCAGTATACTTGAAATACCGGTCTTGCGAAGTAGT
GGTAGCGCATAATGGTAATCTCACTAACACTGATGAGATAAGAACCAAGCTTGAGAGTGAAGGTTGCGTTTTGCAATCTG
AGGTTGATACTGAGGTCATAGCGCACTTAATAGCCCGCTCTAGCAGGAAGACTCCAACAGAAAAAATTGTGGATGCGTTG
CAACAAGTTGAGGGAGCCTATTCATTACTATTGTTTGTTGGAGGAGAGATCTTTGCTGTAAGAGATCCTCATGGAGTAAG
GCCACTTAGTCTTGGTAAACTTGGTGATGGGATTGTCATAGCATCTGAGACCTGTGCGTTGGACATGCTCAAAGCAACTT
TCGTCAGGGATATGGCACCTGGTGAGCTGCTGCAAATAAAAGATGGTAAGCTCACATCTCTGTTCCCGTTTAAGAGAATG
GAACATAAATTTTGTATCTTTGAGCACGTGTATTTCGCTAGACCAGATAGCGTTTTAGAGGGGCGTAGCGTCTACGCCTC
AAGAAAAGAAATAGGGAAAGAACTTGCTCGTGAGAGTAAAATCGAAGCCGATATGGTAGTACCCGTACTTGATTCTGGTG
TGGTAGCAGCCCTTGGTTACTCAGAAGAGTCCAACTTACCGCTCGAACTTGCAATCACAAAGAACCATTATTCAAGCAGG
AGTTTTATAGAACCTACGCCGGAGCGGAGAAACATGAGGGTCAAATTGAAACACAATGCAAATAGATTCTTGCTAAAGGG
AAAGAAGGTGGTTCTTGTGGATGACAGCATAGTGAGGGGGACCACACTTAAGCACCTGATCACTATGTTGTGGGAAGCCG
GAACAAGTGAAATACATGTCAGAATATCAAGCCCACGGATTCTTAATCCATGCTATTACGGAGTTGACACACCTAACAGA
AAGGATCTGATATCGGCAAACCTCCCTTTAGGAGAAATGAGCGTGTATTTAGGCGCAACAAGTTTGTACTTTCTTACCCT
TGAAGGGCTATACAAAGCTGTATCCGGGAGTGAGAAGCGTATAGGTTTTTGTGATGCCTGTTTCACTGGTGATTATCCGA
TAAAGTGCCTTGGGACTTCCCAAAGTACGTGA

Upstream 100 bases:

>100_bases
TTTGATGCCGATCAAAATAGCCAAGAAGTCCTTGTCACGTGGCTTCGGACATCTGCGTAAGCAGAGATTTCTCTTGCGTT
TTGAGGGAAGTTGGTAGGTC

Downstream 100 bases:

>100_bases
CCTATACGGTGTCTCAGTTATAGTGTTACTCGTGCATACAAGGACTATGAGCAGGATCTTCTGTTCCATAAATGTGGCAC
TGCTCTGCAGTTTTGTATTA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MVSRDQSGWLTEKCGVVAVVGTPNAVELSLLGLHGLQHRGHEAFGIAFVHNGDVGVVHRFGRIMSVNPVDLSIPSAETVI
GHVRYSTSGGSDFSQPVYLKYRSCEVVVAHNGNLTNTDEIRTKLESEGCVLQSEVDTEVIAHLIARSSRKTPTEKIVDAL
QQVEGAYSLLLFVGGEIFAVRDPHGVRPLSLGKLGDGIVIASETCALDMLKATFVRDMAPGELLQIKDGKLTSLFPFKRM
EHKFCIFEHVYFARPDSVLEGRSVYASRKEIGKELARESKIEADMVVPVLDSGVVAALGYSEESNLPLELAITKNHYSSR
SFIEPTPERRNMRVKLKHNANRFLLKGKKVVLVDDSIVRGTTLKHLITMLWEAGTSEIHVRISSPRILNPCYYGVDTPNR
KDLISANLPLGEMSVYLGATSLYFLTLEGLYKAVSGSEKRIGFCDACFTGDYPIKCLGTSQST

Sequences:

>Translated_463_residues
MVSRDQSGWLTEKCGVVAVVGTPNAVELSLLGLHGLQHRGHEAFGIAFVHNGDVGVVHRFGRIMSVNPVDLSIPSAETVI
GHVRYSTSGGSDFSQPVYLKYRSCEVVVAHNGNLTNTDEIRTKLESEGCVLQSEVDTEVIAHLIARSSRKTPTEKIVDAL
QQVEGAYSLLLFVGGEIFAVRDPHGVRPLSLGKLGDGIVIASETCALDMLKATFVRDMAPGELLQIKDGKLTSLFPFKRM
EHKFCIFEHVYFARPDSVLEGRSVYASRKEIGKELARESKIEADMVVPVLDSGVVAALGYSEESNLPLELAITKNHYSSR
SFIEPTPERRNMRVKLKHNANRFLLKGKKVVLVDDSIVRGTTLKHLITMLWEAGTSEIHVRISSPRILNPCYYGVDTPNR
KDLISANLPLGEMSVYLGATSLYFLTLEGLYKAVSGSEKRIGFCDACFTGDYPIKCLGTSQST
>Mature_463_residues
MVSRDQSGWLTEKCGVVAVVGTPNAVELSLLGLHGLQHRGHEAFGIAFVHNGDVGVVHRFGRIMSVNPVDLSIPSAETVI
GHVRYSTSGGSDFSQPVYLKYRSCEVVVAHNGNLTNTDEIRTKLESEGCVLQSEVDTEVIAHLIARSSRKTPTEKIVDAL
QQVEGAYSLLLFVGGEIFAVRDPHGVRPLSLGKLGDGIVIASETCALDMLKATFVRDMAPGELLQIKDGKLTSLFPFKRM
EHKFCIFEHVYFARPDSVLEGRSVYASRKEIGKELARESKIEADMVVPVLDSGVVAALGYSEESNLPLELAITKNHYSSR
SFIEPTPERRNMRVKLKHNANRFLLKGKKVVLVDDSIVRGTTLKHLITMLWEAGTSEIHVRISSPRILNPCYYGVDTPNR
KDLISANLPLGEMSVYLGATSLYFLTLEGLYKAVSGSEKRIGFCDACFTGDYPIKCLGTSQST

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=507, Percent_Identity=34.1222879684418, Blast_Score=275, Evalue=5e-74,
Organism=Escherichia coli, GI1788651, Length=469, Percent_Identity=34.1151385927505, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI1790167, Length=163, Percent_Identity=31.2883435582822, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17554892, Length=476, Percent_Identity=34.453781512605, Blast_Score=275, Evalue=3e-74,
Organism=Saccharomyces cerevisiae, GI6323958, Length=474, Percent_Identity=33.1223628691983, Blast_Score=196, Evalue=1e-50,
Organism=Drosophila melanogaster, GI24659598, Length=496, Percent_Identity=35.6854838709677, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24659604, Length=464, Percent_Identity=36.4224137931034, Blast_Score=287, Evalue=9e-78,
Organism=Drosophila melanogaster, GI28573187, Length=490, Percent_Identity=35.1020408163265, Blast_Score=281, Evalue=5e-76,
Organism=Drosophila melanogaster, GI21357745, Length=181, Percent_Identity=30.939226519337, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 50842; Mature: 50842

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSRDQSGWLTEKCGVVAVVGTPNAVELSLLGLHGLQHRGHEAFGIAFVHNGDVGVVHRF
CCCCCCCCCEECCCCEEEEECCCCEEEEEEEECHHHCCCCCCEEEEEEEECCCCHHHHHC
GRIMSVNPVDLSIPSAETVIGHVRYSTSGGSDFSQPVYLKYRSCEVVVAHNGNLTNTDEI
CCEEECCCCEEECCCHHHEEEEEEEECCCCCCCCCCEEEEEECEEEEEEECCCCCCHHHH
RTKLESEGCVLQSEVDTEVIAHLIARSSRKTPTEKIVDALQQVEGAYSLLLFVGGEIFAV
HHHHCCCCCEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEEECCEEEEE
RDPHGVRPLSLGKLGDGIVIASETCALDMLKATFVRDMAPGELLQIKDGKLTSLFPFKRM
ECCCCCCCEECCCCCCCEEEEECHHHHHHHHHHHHHCCCCCCEEEECCCCEEEECCHHHH
EHKFCIFEHVYFARPDSVLEGRSVYASRKEIGKELARESKIEADMVVPVLDSGVVAALGY
CCCEEEEEEEEECCCCCHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEECC
SEESNLPLELAITKNHYSSRSFIEPTPERRNMRVKLKHNANRFLLKGKKVVLVDDSIVRG
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEECCEEEEECCCCCCC
TTLKHLITMLWEAGTSEIHVRISSPRILNPCYYGVDTPNRKDLISANLPLGEMSVYLGAT
HHHHHHHHHHHHCCCCEEEEEECCCCEECCEEECCCCCCCCCEEECCCCCCCEEEEECCC
SLYFLTLEGLYKAVSGSEKRIGFCDACFTGDYPIKCLGTSQST
EEEEEEHHHHHHHHCCCCCCCEEEEEEECCCCCEEEECCCCCC
>Mature Secondary Structure
MVSRDQSGWLTEKCGVVAVVGTPNAVELSLLGLHGLQHRGHEAFGIAFVHNGDVGVVHRF
CCCCCCCCCEECCCCEEEEECCCCEEEEEEEECHHHCCCCCCEEEEEEEECCCCHHHHHC
GRIMSVNPVDLSIPSAETVIGHVRYSTSGGSDFSQPVYLKYRSCEVVVAHNGNLTNTDEI
CCEEECCCCEEECCCHHHEEEEEEEECCCCCCCCCCEEEEEECEEEEEEECCCCCCHHHH
RTKLESEGCVLQSEVDTEVIAHLIARSSRKTPTEKIVDALQQVEGAYSLLLFVGGEIFAV
HHHHCCCCCEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEEECCEEEEE
RDPHGVRPLSLGKLGDGIVIASETCALDMLKATFVRDMAPGELLQIKDGKLTSLFPFKRM
ECCCCCCCEECCCCCCCEEEEECHHHHHHHHHHHHHCCCCCCEEEECCCCEEEECCHHHH
EHKFCIFEHVYFARPDSVLEGRSVYASRKEIGKELARESKIEADMVVPVLDSGVVAALGY
CCCEEEEEEEEECCCCCHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEECC
SEESNLPLELAITKNHYSSRSFIEPTPERRNMRVKLKHNANRFLLKGKKVVLVDDSIVRG
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEECCEEEEECCCCCCC
TTLKHLITMLWEAGTSEIHVRISSPRILNPCYYGVDTPNRKDLISANLPLGEMSVYLGAT
HHHHHHHHHHHHCCCCEEEEEECCCCEECCEEECCCCCCCCCEEECCCCCCCEEEEECCC
SLYFLTLEGLYKAVSGSEKRIGFCDACFTGDYPIKCLGTSQST
EEEEEEHHHHHHHHCCCCCCCEEEEEEECCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]