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The map label for this gene is carA

Identifier: 88608787

GI number: 88608787

Start: 422881

End: 423978

Strand: Reverse

Name: carA

Synonym: NSE_0502

Alternate gene names: 88608787

Gene position: 423978-422881 (Counterclockwise)

Preceding gene: 88608668

Following gene: 88608470

Centisome position: 49.36

GC content: 40.44

Gene sequence:

>1098_bases
TTGTCATACGATAGCTGTCTTGCTTTTGCTGGTGGTGAGTGCTTTTTTGGATTTTCGTATGGTAAAAGGGATGTCGCAGT
TGGTGAAGTATGTTTCACTACCGGGATGACAGGTTACCAGCATACCATCACCGATCCCTCTTTTTCCGGTCAAATCATCC
TTTTTGCTTTTCCCCATATTGGAAATGTTGCTATTAATTCCTGCGATAACGAGTCTTCCCGTATTTTTGCTAGGGGACTG
GTTTTTCGAGAAGGGCCACAGGATTTTTTGCATCATTTGAGGATGAGGAGCTTTTCTAGCTGGCTAGAAGATAATAATGT
TACAGCAATATATGGAGTTGACACACGTGCGATTACTCGGTTGGTTCGCCTAAAGGGCAATCAGGGCGGTATCATTTTTC
CTGTTAATGACATCAGTATAGATGAAGCTATCTTGCTTCTTAGGGAAACTGAGGATATGAATGGAAATGAGTTAGCTACG
TCTGCAAGTGGAAATGCACGATGCTCACCTTGCTGTGTTGGTGATAAGAGAAAAAAAGTCTGTGTAGTCGACTTCGGCAT
AAAGGATGGTATCGTCAGAAATCTGGAAAAATATTTTACAGTTGTGGTAGTAGATGGAAAAAAAGGGTTTTCATCTGCTT
TGGAGTCAGAGGACTTTGCTGGCATCGTCCTCTCGAATGGTCCAGGTGATCCGTCTGCGACATACTTACAATTGTGTGAG
GATTTTTCAAGAGTTCTTACCTCTAATTTACCAGTCTTGGGGATCTGTCTAGGACATCAGTTACTGCTTCTTGCATTTGG
ATGCAAAACAAAAAAGATGCTTGTAGGACATAGAGGAACTAACCACCCCGTTATCAATCTTGAGACACAAAGGGTAGAAA
TCACAAGTCAAAATCATGGTTTTGTATTAGATGAATCGACATTAAATAGTAAAGATATTATTGTTACACACAGATCCCTG
TTCGATGGTTCTGTAGAGGGGATCAAGATAAAAGACAGGAAAATCTTTTCCGTTCAATATCATCCTGAAGGTTCACCAGG
ACCGCATGACTCTCACTACATCTTTAGTAAATTTTTTAATGAAATTAGTTCTCAATAG

Upstream 100 bases:

>100_bases
AATCCGCCTCTACAAGCATTAAGTCCACTAGTAAAGTCAACAAATTCAAAGAGAAGTTTGGAGAGGACTTCATCTAATTT
ACTCGAGGATGAACAAGAAT

Downstream 100 bases:

>100_bases
AATTGCTCTACATACCGGATTAGGAATCCGTTCTTGGGAAAGGGTTGTAAAAAGAATAGTTTTGATTAACATTACTCTCC
ATTCAGATAAGCTACTTGAT

Product: carbamoyl-phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MSYDSCLAFAGGECFFGFSYGKRDVAVGEVCFTTGMTGYQHTITDPSFSGQIILFAFPHIGNVAINSCDNESSRIFARGL
VFREGPQDFLHHLRMRSFSSWLEDNNVTAIYGVDTRAITRLVRLKGNQGGIIFPVNDISIDEAILLLRETEDMNGNELAT
SASGNARCSPCCVGDKRKKVCVVDFGIKDGIVRNLEKYFTVVVVDGKKGFSSALESEDFAGIVLSNGPGDPSATYLQLCE
DFSRVLTSNLPVLGICLGHQLLLLAFGCKTKKMLVGHRGTNHPVINLETQRVEITSQNHGFVLDESTLNSKDIIVTHRSL
FDGSVEGIKIKDRKIFSVQYHPEGSPGPHDSHYIFSKFFNEISSQ

Sequences:

>Translated_365_residues
MSYDSCLAFAGGECFFGFSYGKRDVAVGEVCFTTGMTGYQHTITDPSFSGQIILFAFPHIGNVAINSCDNESSRIFARGL
VFREGPQDFLHHLRMRSFSSWLEDNNVTAIYGVDTRAITRLVRLKGNQGGIIFPVNDISIDEAILLLRETEDMNGNELAT
SASGNARCSPCCVGDKRKKVCVVDFGIKDGIVRNLEKYFTVVVVDGKKGFSSALESEDFAGIVLSNGPGDPSATYLQLCE
DFSRVLTSNLPVLGICLGHQLLLLAFGCKTKKMLVGHRGTNHPVINLETQRVEITSQNHGFVLDESTLNSKDIIVTHRSL
FDGSVEGIKIKDRKIFSVQYHPEGSPGPHDSHYIFSKFFNEISSQ
>Mature_364_residues
SYDSCLAFAGGECFFGFSYGKRDVAVGEVCFTTGMTGYQHTITDPSFSGQIILFAFPHIGNVAINSCDNESSRIFARGLV
FREGPQDFLHHLRMRSFSSWLEDNNVTAIYGVDTRAITRLVRLKGNQGGIIFPVNDISIDEAILLLRETEDMNGNELATS
ASGNARCSPCCVGDKRKKVCVVDFGIKDGIVRNLEKYFTVVVVDGKKGFSSALESEDFAGIVLSNGPGDPSATYLQLCED
FSRVLTSNLPVLGICLGHQLLLLAFGCKTKKMLVGHRGTNHPVINLETQRVEITSQNHGFVLDESTLNSKDIIVTHRSLF
DGSVEGIKIKDRKIFSVQYHPEGSPGPHDSHYIFSKFFNEISSQ

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI21361331, Length=377, Percent_Identity=33.42175066313, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI169790915, Length=377, Percent_Identity=33.42175066313, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI18105007, Length=370, Percent_Identity=35.1351351351351, Blast_Score=184, Evalue=9e-47,
Organism=Escherichia coli, GI1786215, Length=377, Percent_Identity=41.1140583554377, Blast_Score=260, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI193204318, Length=377, Percent_Identity=32.8912466843501, Blast_Score=182, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6324878, Length=364, Percent_Identity=34.8901098901099, Blast_Score=196, Evalue=7e-51,
Organism=Saccharomyces cerevisiae, GI6322331, Length=393, Percent_Identity=33.0788804071247, Blast_Score=193, Evalue=3e-50,
Organism=Drosophila melanogaster, GI45555749, Length=389, Percent_Identity=32.9048843187661, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24642586, Length=389, Percent_Identity=32.9048843187661, Blast_Score=186, Evalue=3e-47,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 40143; Mature: 40012

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYDSCLAFAGGECFFGFSYGKRDVAVGEVCFTTGMTGYQHTITDPSFSGQIILFAFPHI
CCCCCEEEEECCCEEEEECCCCCCCEEHHHHHHCCCCCCEEECCCCCCCCEEEEEECCCC
GNVAINSCDNESSRIFARGLVFREGPQDFLHHLRMRSFSSWLEDNNVTAIYGVDTRAITR
CCEEEECCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHH
LVRLKGNQGGIIFPVNDISIDEAILLLRETEDMNGNELATSASGNARCSPCCVGDKRKKV
HHHEECCCCCEEEEECCCCCCCEEEEEEECCCCCCCEEEECCCCCCCCCCEECCCCCCEE
CVVDFGIKDGIVRNLEKYFTVVVVDGKKGFSSALESEDFAGIVLSNGPGDPSATYLQLCE
EEEECCCCHHHHHHHHCEEEEEEEECCCCHHHHHCCCCCEEEEEECCCCCCHHHHHHHHH
DFSRVLTSNLPVLGICLGHQLLLLAFGCKTKKMLVGHRGTNHPVINLETQRVEITSQNHG
HHHHHHHCCCCEEEEHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEEEEEEECCCCC
FVLDESTLNSKDIIVTHRSLFDGSVEGIKIKDRKIFSVQYHPEGSPGPHDSHYIFSKFFN
EEEECCCCCCCEEEEEECHHCCCCCCCEEEECCEEEEEEECCCCCCCCCCCHHHHHHHHH
EISSQ
HHHCC
>Mature Secondary Structure 
SYDSCLAFAGGECFFGFSYGKRDVAVGEVCFTTGMTGYQHTITDPSFSGQIILFAFPHI
CCCCEEEEECCCEEEEECCCCCCCEEHHHHHHCCCCCCEEECCCCCCCCEEEEEECCCC
GNVAINSCDNESSRIFARGLVFREGPQDFLHHLRMRSFSSWLEDNNVTAIYGVDTRAITR
CCEEEECCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHH
LVRLKGNQGGIIFPVNDISIDEAILLLRETEDMNGNELATSASGNARCSPCCVGDKRKKV
HHHEECCCCCEEEEECCCCCCCEEEEEEECCCCCCCEEEECCCCCCCCCCEECCCCCCEE
CVVDFGIKDGIVRNLEKYFTVVVVDGKKGFSSALESEDFAGIVLSNGPGDPSATYLQLCE
EEEECCCCHHHHHHHHCEEEEEEEECCCCHHHHHCCCCCEEEEEECCCCCCHHHHHHHHH
DFSRVLTSNLPVLGICLGHQLLLLAFGCKTKKMLVGHRGTNHPVINLETQRVEITSQNHG
HHHHHHHCCCCEEEEHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEEEEEEECCCCC
FVLDESTLNSKDIIVTHRSLFDGSVEGIKIKDRKIFSVQYHPEGSPGPHDSHYIFSKFFN
EEEECCCCCCCEEEEEECHHCCCCCCCEEEECCEEEEEEECCCCCCCCCCCHHHHHHHHH
EISSQ
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA