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The map label for this gene is gltX-1

Identifier: 88608755

GI number: 88608755

Start: 441445

End: 442746

Strand: Reverse

Name: gltX-1

Synonym: NSE_0518

Alternate gene names: 88608755

Gene position: 442746-441445 (Counterclockwise)

Preceding gene: 88608371

Following gene: 88608701

Centisome position: 51.54

GC content: 38.48

Gene sequence:

>1302_bases
ATGATTACTCGTTTTGCACCAAGTCCGACTGGGCTTATTCACTTAGGAAATGCAAGAACAGCGCTAATTGCATACTTTGC
AGCTCGATCTTCTGGAGGTGAATTTATTTTAAGAATCGATGATACGGATGTCACAAGAATCAAAAGTGAGTACGTGAACA
AGATTTTTACAGACCTATCATGGCTTGGGATAAAGGAAGATCTTTGTATAAAACAGTCTGAGCGATGTGAGCTTTATGCA
AATGCAGTAGTTAAGCTCAAAGAAAGTGGCAGGATTTATCCTTGTTATGAGACGCCTGAAGAGCTCGAAATAGAGCGTAG
ATCGCTTTTGGCTCGTGCTCTTCCACCTGTATACAGAAGAAAAAATAGACCTCAACATTCAACTCGACTCCCATACTATC
GTTTTGAACTAGACCCTGATCGAGTGGTTATATGGGAAGATAAACTTCGAGGAAAAATAGTAATAGATTTGCATAGCACG
AGTGATCCGATTGTCATAAGAGAAAACGGCACTTATACGTATATGTTACCTTCAGTTGTTGATGATATTGAGTGCAGAAT
TAGTACTATTATCCGTGGTGAGGACCATATCTCTAATACTGCTGTGCAGATACAGATGTTTGAAGCACTGGGGTGCACTG
AAATTCCAAAGTTTGCTCATATGCCACTACTTAAACTGCAAACTGGAAAGATGTCTAAAAGAGCTGGCGGTAACGAAATA
CAAACTTTTAGGGAAAACTACATAGAACCCGAAGTGATATGTCTGTACTTGTTAAATTTGGGCAGTAAGTCTCGAAGTAC
TTTCAAGAATTATAAGGATTATAGCAATTTTAATCTAGAAAATTATTCTTCATCTTCGTCAGTGACTGTTTTGGAAGATG
AAATTTATAGCCTTAACGCTGATTTTTTACGTCTTTCTAACTATGAGGATCTTGCAAGACGTTTGGGTGGTGTGTCGAAG
ACTTTCTGGGATGCTGTTAAAAACAATGTAAAAAGTATTCCTGAAGTAAATTACTGGTGGGAAATATGTACTACTGAGGC
TAAGCTTTACCCTGGTATTGGATGCAATGCACAACTTGTTAGAGACGCTATATCAGTACTTCCTCAGGAAGAAGTCTCTA
GAGAGACATATAGGCGGTGGGTTGAGCTAATTGTGGAAAACTATAGATATGATAAGAGAGAGGTTCATATCAATCTGAGA
CTTGCGTTAACTGGGAAAGTAGGTGGTCCAGAAATGGCAGCCATTCTTCCGTTCATAAGTAGAGAGAGAATATTAATCAG
ATTGAATGATTCACTTAGTTAA

Upstream 100 bases:

>100_bases
TTATGATGGATAATCAGCGTATTTGCTTTCATTCTTTCAGTGAAGACTTTAGTCCTGGGATATTTGAGGTGAAGCACCAT
ATTTCCAATTCTCTTCTCAG

Downstream 100 bases:

>100_bases
TGTTTGAATTAATAACACTGTACATTTTGTTGATTGTACTCTTTCAAAGCAGCAAGATCAGGCGTCATGCGAAAAGCAAA
GACTTTTTTATTGATTGTTT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MITRFAPSPTGLIHLGNARTALIAYFAARSSGGEFILRIDDTDVTRIKSEYVNKIFTDLSWLGIKEDLCIKQSERCELYA
NAVVKLKESGRIYPCYETPEELEIERRSLLARALPPVYRRKNRPQHSTRLPYYRFELDPDRVVIWEDKLRGKIVIDLHST
SDPIVIRENGTYTYMLPSVVDDIECRISTIIRGEDHISNTAVQIQMFEALGCTEIPKFAHMPLLKLQTGKMSKRAGGNEI
QTFRENYIEPEVICLYLLNLGSKSRSTFKNYKDYSNFNLENYSSSSSVTVLEDEIYSLNADFLRLSNYEDLARRLGGVSK
TFWDAVKNNVKSIPEVNYWWEICTTEAKLYPGIGCNAQLVRDAISVLPQEEVSRETYRRWVELIVENYRYDKREVHINLR
LALTGKVGGPEMAAILPFISRERILIRLNDSLS

Sequences:

>Translated_433_residues
MITRFAPSPTGLIHLGNARTALIAYFAARSSGGEFILRIDDTDVTRIKSEYVNKIFTDLSWLGIKEDLCIKQSERCELYA
NAVVKLKESGRIYPCYETPEELEIERRSLLARALPPVYRRKNRPQHSTRLPYYRFELDPDRVVIWEDKLRGKIVIDLHST
SDPIVIRENGTYTYMLPSVVDDIECRISTIIRGEDHISNTAVQIQMFEALGCTEIPKFAHMPLLKLQTGKMSKRAGGNEI
QTFRENYIEPEVICLYLLNLGSKSRSTFKNYKDYSNFNLENYSSSSSVTVLEDEIYSLNADFLRLSNYEDLARRLGGVSK
TFWDAVKNNVKSIPEVNYWWEICTTEAKLYPGIGCNAQLVRDAISVLPQEEVSRETYRRWVELIVENYRYDKREVHINLR
LALTGKVGGPEMAAILPFISRERILIRLNDSLS
>Mature_433_residues
MITRFAPSPTGLIHLGNARTALIAYFAARSSGGEFILRIDDTDVTRIKSEYVNKIFTDLSWLGIKEDLCIKQSERCELYA
NAVVKLKESGRIYPCYETPEELEIERRSLLARALPPVYRRKNRPQHSTRLPYYRFELDPDRVVIWEDKLRGKIVIDLHST
SDPIVIRENGTYTYMLPSVVDDIECRISTIIRGEDHISNTAVQIQMFEALGCTEIPKFAHMPLLKLQTGKMSKRAGGNEI
QTFRENYIEPEVICLYLLNLGSKSRSTFKNYKDYSNFNLENYSSSSSVTVLEDEIYSLNADFLRLSNYEDLARRLGGVSK
TFWDAVKNNVKSIPEVNYWWEICTTEAKLYPGIGCNAQLVRDAISVLPQEEVSRETYRRWVELIVENYRYDKREVHINLR
LALTGKVGGPEMAAILPFISRERILIRLNDSLS

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=338, Percent_Identity=33.1360946745562, Blast_Score=151, Evalue=1e-36,
Organism=Escherichia coli, GI1788743, Length=462, Percent_Identity=29.2207792207792, Blast_Score=181, Evalue=9e-47,
Organism=Escherichia coli, GI226510925, Length=278, Percent_Identity=29.136690647482, Blast_Score=96, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI71993394, Length=468, Percent_Identity=26.2820512820513, Blast_Score=139, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6324540, Length=329, Percent_Identity=32.2188449848024, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6321192, Length=114, Percent_Identity=35.0877192982456, Blast_Score=76, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24665321, Length=341, Percent_Identity=32.258064516129, Blast_Score=142, Evalue=3e-34,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE1_NEOSM (Q2GDP4)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506402.1
- ProteinModelPortal:   Q2GDP4
- SMR:   Q2GDP4
- STRING:   Q2GDP4
- GeneID:   3931642
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0518
- TIGR:   NSE_0518
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   RSKQEYI
- BioCyc:   NSEN222891:NSE_0518-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 49867; Mature: 49867

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 233-233

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITRFAPSPTGLIHLGNARTALIAYFAARSSGGEFILRIDDTDVTRIKSEYVNKIFTDLS
CCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHH
WLGIKEDLCIKQSERCELYANAVVKLKESGRIYPCYETPEELEIERRSLLARALPPVYRR
HCCCCHHHHCCCCCCHHEEEHEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHCCHHHHH
KNRPQHSTRLPYYRFELDPDRVVIWEDKLRGKIVIDLHSTSDPIVIRENGTYTYMLPSVV
CCCCCCCCCCCEEEEEECCCEEEEEECCCCCEEEEEEECCCCCEEEEECCEEEEECHHHH
DDIECRISTIIRGEDHISNTAVQIQMFEALGCTEIPKFAHMPLLKLQTGKMSKRAGGNEI
HHHHHEEEEEECCCCCCCCCEEEEEEEHHCCCCCCCHHHCCCEEEEECCCCHHCCCCCHH
QTFRENYIEPEVICLYLLNLGSKSRSTFKNYKDYSNFNLENYSSSSSVTVLEDEIYSLNA
HHHHHHCCCHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEECHHHCCCC
DFLRLSNYEDLARRLGGVSKTFWDAVKNNVKSIPEVNYWWEICTTEAKLYPGIGCNAQLV
CCEEECCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCEECCCCCCCHHHH
RDAISVLPQEEVSRETYRRWVELIVENYRYDKREVHINLRLALTGKVGGPEMAAILPFIS
HHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHC
RERILIRLNDSLS
CCEEEEEECCCCC
>Mature Secondary Structure
MITRFAPSPTGLIHLGNARTALIAYFAARSSGGEFILRIDDTDVTRIKSEYVNKIFTDLS
CCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHH
WLGIKEDLCIKQSERCELYANAVVKLKESGRIYPCYETPEELEIERRSLLARALPPVYRR
HCCCCHHHHCCCCCCHHEEEHEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHCCHHHHH
KNRPQHSTRLPYYRFELDPDRVVIWEDKLRGKIVIDLHSTSDPIVIRENGTYTYMLPSVV
CCCCCCCCCCCEEEEEECCCEEEEEECCCCCEEEEEEECCCCCEEEEECCEEEEECHHHH
DDIECRISTIIRGEDHISNTAVQIQMFEALGCTEIPKFAHMPLLKLQTGKMSKRAGGNEI
HHHHHEEEEEECCCCCCCCCEEEEEEEHHCCCCCCCHHHCCCEEEEECCCCHHCCCCCHH
QTFRENYIEPEVICLYLLNLGSKSRSTFKNYKDYSNFNLENYSSSSSVTVLEDEIYSLNA
HHHHHHCCCHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEECHHHCCCC
DFLRLSNYEDLARRLGGVSKTFWDAVKNNVKSIPEVNYWWEICTTEAKLYPGIGCNAQLV
CCEEECCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCEECCCCCCCHHHH
RDAISVLPQEEVSRETYRRWVELIVENYRYDKREVHINLRLALTGKVGGPEMAAILPFIS
HHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHC
RERILIRLNDSLS
CCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA