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The map label for this gene is glyS

Identifier: 88608720

GI number: 88608720

Start: 672338

End: 674428

Strand: Reverse

Name: glyS

Synonym: NSE_0756

Alternate gene names: 88608720

Gene position: 674428-672338 (Counterclockwise)

Preceding gene: 88608155

Following gene: 88608238

Centisome position: 78.51

GC content: 40.94

Gene sequence:

>2091_bases
ATGCATAAGTTTCTTCTAGAAATTTTGTCTGAAGAAATGCCTGTCGCTCTGCAAAGTTATGCGCAGAAAAGTTTTTTTGA
CCTTTTTTCATCTGCCACTAAGGACCTAAGTGTTGGAAAGATAGAAGTCTTTACTAGTTGCAGGAGGATAGCAGTACTCA
TAGATGATGTAAAAAGCGATCTCGTAAATCAAAAAGTTGATAAAAAGGGTCCAAGTGTTGGTTCAGATCTTTCTGTTGTG
GAGAGGTTCGCCACTTCAATGGGTGCTAGTACTGCGGATTTATATCTCAAGAGTATTGGAAATAAAGAATATTATTTTGC
TCCAGTTGCAGGAAAAGTGCTCCACATAGATGACATTATGAGACAACGGACCGAGGCTATACTCAGGAGTTTTTCATGGC
CTAGGTCAATGCGATGGGGGAATTATTCTCTGAACTGGATCAGGCCCATAAAAAGCATTGTTTGTATGCTTGGAAAATCC
ATCATTCCCGTGCAGTTCCATCATTATACTGCGGGAGCGATAACAGTGGGTCCTTATGTTGATCCTGCCTTGCAAAAAAT
CAAAATCACATCTGCTGAGGAATATCAATCCATTTTGATGAAAGGGGGTGTTATTCTGTCCCAGATTGAAAGGGAGAACC
TTATAAAAGAGCAAATATCTCAGCTAGAGGCTGAGCATGGATTTTCTGTTTGCGTGACGGAGGAGTTATTGAATGAGGTT
GTTGGAATTACCGAATCGCCTAATGTGCTCCTGGGATCGTTCCCGCAGTCATTTCTGGAGCTCCATGAACGCATAATAGA
AACAGTTATTGTTTCTCACCAGAAAGTTTTTCCGGTGTCAAAGAATGGTAAGCTCCTGAATAAATTTGTGTTTGTTAGTT
CGATTGCTGGCGATGCACCAATTAGGGGATATGAGCGCGTGATTACAGCAAGATTAGCTGATGCAGCGTCAATGGTTCTG
ATTGATTCTAAAAATCCCTTGCGCGAACTAAGTCCAAAACTAAAACAGCTGATTTTTCACAAGAAATTGGGTAGCTTTAG
TGAGAAAGTACAGCGTGTTAGGGCATTGGCAAAGTTTATTGCGTTTTGGGTGCCAAACGCTTCCATTATAAAAGTGGAAG
AAGCTGCTAAACTTGCTAAGCTGGATTTATTGACAACGTTTGTCCGTGATTTTCCCAAGCTGGCTGGTTTTATGGGCTCT
CACTGCTTAAGCCTGTGTGGTGAGACAGATGTTGAAATTATTGATGCAGTTAGGGATCATTATTTGCCTGTTGATGCGGC
AGATATTTGCCCGACTGCTCCCGTCACAGTGACGCTTGCACTAGCTGATAAAATAGATACTTTAGTTGGCTTGATCAGCG
TAAAAGAGAAAGTTACTTCCACTCGTGATCCGCTTGGTATAAGGCGCGCAGCTATTGGTGTGTTGCGTATCATCATAGAA
AATAAACTGGATGTTCCACTCCAGGTCTTCTTAATGAAAGCGATTGATTTGTATCCCTCGTCTTTATTTTTTTCTCTTAT
CAAAAAGATTGGTGCTAGCTCTGATGAAGAGAAGCAACGGAAAGATGATGTTGTGAATTTCACATTAAGTTTCTTTTCCG
AGCGTCTAAAGGCGCTGCTATTTAAAAGTGGTGTGAGCCAAGCAGTTTTTGCTTCGGTTAGTAGCGGAGTTACGCGTCCT
CTGGTGCTAACGGAGAAGTGTATTGCTATTTCTGAATGGCTCAAGCAAGCCGAGGGTGCTGAATTCATGCGGCTTCATAA
GAGGATGATGGGTATCCTTAAGGGCTTTTCTGGTTCTGCTAAAGCAAAGTTTAGTAAAAAAGTACTCGTTCTACCAGAAG
AGGTGAGTCTGGTACAAGAGTTTGAGGGTTTTCAATCACTTTTTTGTAATGCCATAAAGGAGCGGAATTTTGAAATGGCA
TTTTCACGGTGTGCTGATCTTTTAAAGCCAATCACGGTTTTTATGGATAGTGTGCTTGTTAATGTTGAAGATCCTGAACT
TAAGAAGAATCGTATTGGGTTGTTACTCTCAATAAGTGAAACTTTAAATGAATTATGTAATTTTGATGTCCTGTCCAGGA
ACTTTTCATGA

Upstream 100 bases:

>100_bases
AGGGGTTTCATAAGCGTCAGTGAACGAGCTAAGTACATCTTGGACATCAGGGGCTTGGTGAAGCAATGTTGTGAGCTTTT
TATAGAGCAGGAGGGGTCTT

Downstream 100 bases:

>100_bases
GTCCTTTATTTTCCTGAGGAGCTGATTCAGTTGTTTTTTCTCATTCATAAATTTTTGAGCTCTTCTCCTTACGTAATATG
GGTCATATCCTGCGAGTGCG

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 696; Mature: 696

Protein sequence:

>696_residues
MHKFLLEILSEEMPVALQSYAQKSFFDLFSSATKDLSVGKIEVFTSCRRIAVLIDDVKSDLVNQKVDKKGPSVGSDLSVV
ERFATSMGASTADLYLKSIGNKEYYFAPVAGKVLHIDDIMRQRTEAILRSFSWPRSMRWGNYSLNWIRPIKSIVCMLGKS
IIPVQFHHYTAGAITVGPYVDPALQKIKITSAEEYQSILMKGGVILSQIERENLIKEQISQLEAEHGFSVCVTEELLNEV
VGITESPNVLLGSFPQSFLELHERIIETVIVSHQKVFPVSKNGKLLNKFVFVSSIAGDAPIRGYERVITARLADAASMVL
IDSKNPLRELSPKLKQLIFHKKLGSFSEKVQRVRALAKFIAFWVPNASIIKVEEAAKLAKLDLLTTFVRDFPKLAGFMGS
HCLSLCGETDVEIIDAVRDHYLPVDAADICPTAPVTVTLALADKIDTLVGLISVKEKVTSTRDPLGIRRAAIGVLRIIIE
NKLDVPLQVFLMKAIDLYPSSLFFSLIKKIGASSDEEKQRKDDVVNFTLSFFSERLKALLFKSGVSQAVFASVSSGVTRP
LVLTEKCIAISEWLKQAEGAEFMRLHKRMMGILKGFSGSAKAKFSKKVLVLPEEVSLVQEFEGFQSLFCNAIKERNFEMA
FSRCADLLKPITVFMDSVLVNVEDPELKKNRIGLLLSISETLNELCNFDVLSRNFS

Sequences:

>Translated_696_residues
MHKFLLEILSEEMPVALQSYAQKSFFDLFSSATKDLSVGKIEVFTSCRRIAVLIDDVKSDLVNQKVDKKGPSVGSDLSVV
ERFATSMGASTADLYLKSIGNKEYYFAPVAGKVLHIDDIMRQRTEAILRSFSWPRSMRWGNYSLNWIRPIKSIVCMLGKS
IIPVQFHHYTAGAITVGPYVDPALQKIKITSAEEYQSILMKGGVILSQIERENLIKEQISQLEAEHGFSVCVTEELLNEV
VGITESPNVLLGSFPQSFLELHERIIETVIVSHQKVFPVSKNGKLLNKFVFVSSIAGDAPIRGYERVITARLADAASMVL
IDSKNPLRELSPKLKQLIFHKKLGSFSEKVQRVRALAKFIAFWVPNASIIKVEEAAKLAKLDLLTTFVRDFPKLAGFMGS
HCLSLCGETDVEIIDAVRDHYLPVDAADICPTAPVTVTLALADKIDTLVGLISVKEKVTSTRDPLGIRRAAIGVLRIIIE
NKLDVPLQVFLMKAIDLYPSSLFFSLIKKIGASSDEEKQRKDDVVNFTLSFFSERLKALLFKSGVSQAVFASVSSGVTRP
LVLTEKCIAISEWLKQAEGAEFMRLHKRMMGILKGFSGSAKAKFSKKVLVLPEEVSLVQEFEGFQSLFCNAIKERNFEMA
FSRCADLLKPITVFMDSVLVNVEDPELKKNRIGLLLSISETLNELCNFDVLSRNFS
>Mature_696_residues
MHKFLLEILSEEMPVALQSYAQKSFFDLFSSATKDLSVGKIEVFTSCRRIAVLIDDVKSDLVNQKVDKKGPSVGSDLSVV
ERFATSMGASTADLYLKSIGNKEYYFAPVAGKVLHIDDIMRQRTEAILRSFSWPRSMRWGNYSLNWIRPIKSIVCMLGKS
IIPVQFHHYTAGAITVGPYVDPALQKIKITSAEEYQSILMKGGVILSQIERENLIKEQISQLEAEHGFSVCVTEELLNEV
VGITESPNVLLGSFPQSFLELHERIIETVIVSHQKVFPVSKNGKLLNKFVFVSSIAGDAPIRGYERVITARLADAASMVL
IDSKNPLRELSPKLKQLIFHKKLGSFSEKVQRVRALAKFIAFWVPNASIIKVEEAAKLAKLDLLTTFVRDFPKLAGFMGS
HCLSLCGETDVEIIDAVRDHYLPVDAADICPTAPVTVTLALADKIDTLVGLISVKEKVTSTRDPLGIRRAAIGVLRIIIE
NKLDVPLQVFLMKAIDLYPSSLFFSLIKKIGASSDEEKQRKDDVVNFTLSFFSERLKALLFKSGVSQAVFASVSSGVTRP
LVLTEKCIAISEWLKQAEGAEFMRLHKRMMGILKGFSGSAKAKFSKKVLVLPEEVSLVQEFEGFQSLFCNAIKERNFEMA
FSRCADLLKPITVFMDSVLVNVEDPELKKNRIGLLLSISETLNELCNFDVLSRNFS

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=708, Percent_Identity=29.0960451977401, Blast_Score=269, Evalue=5e-73,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 77523; Mature: 77523

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKFLLEILSEEMPVALQSYAQKSFFDLFSSATKDLSVGKIEVFTSCRRIAVLIDDVKSD
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LVNQKVDKKGPSVGSDLSVVERFATSMGASTADLYLKSIGNKEYYFAPVAGKVLHIDDIM
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCCEEHHHHHH
RQRTEAILRSFSWPRSMRWGNYSLNWIRPIKSIVCMLGKSIIPVQFHHYTAGAITVGPYV
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEECCCC
DPALQKIKITSAEEYQSILMKGGVILSQIERENLIKEQISQLEAEHGFSVCVTEELLNEV
CHHHHHEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHH
VGITESPNVLLGSFPQSFLELHERIIETVIVSHQKVFPVSKNGKLLNKFVFVSSIAGDAP
HCCCCCCCCEECCCCHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHCCCCC
IRGYERVITARLADAASMVLIDSKNPLRELSPKLKQLIFHKKLGSFSEKVQRVRALAKFI
CHHHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
AFWVPNASIIKVEEAAKLAKLDLLTTFVRDFPKLAGFMGSHCLSLCGETDVEIIDAVRDH
HHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
YLPVDAADICPTAPVTVTLALADKIDTLVGLISVKEKVTSTRDPLGIRRAAIGVLRIIIE
CCCCCHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
NKLDVPLQVFLMKAIDLYPSSLFFSLIKKIGASSDEEKQRKDDVVNFTLSFFSERLKALL
CCCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FKSGVSQAVFASVSSGVTRPLVLTEKCIAISEWLKQAEGAEFMRLHKRMMGILKGFSGSA
HHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
KAKFSKKVLVLPEEVSLVQEFEGFQSLFCNAIKERNFEMAFSRCADLLKPITVFMDSVLV
CHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHC
NVEDPELKKNRIGLLLSISETLNELCNFDVLSRNFS
CCCCCCCCCCCCCEEEEHHHHHHHHHCCHHHHCCCC
>Mature Secondary Structure
MHKFLLEILSEEMPVALQSYAQKSFFDLFSSATKDLSVGKIEVFTSCRRIAVLIDDVKSD
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LVNQKVDKKGPSVGSDLSVVERFATSMGASTADLYLKSIGNKEYYFAPVAGKVLHIDDIM
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCCEEHHHHHH
RQRTEAILRSFSWPRSMRWGNYSLNWIRPIKSIVCMLGKSIIPVQFHHYTAGAITVGPYV
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEECCCC
DPALQKIKITSAEEYQSILMKGGVILSQIERENLIKEQISQLEAEHGFSVCVTEELLNEV
CHHHHHEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHH
VGITESPNVLLGSFPQSFLELHERIIETVIVSHQKVFPVSKNGKLLNKFVFVSSIAGDAP
HCCCCCCCCEECCCCHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHCCCCC
IRGYERVITARLADAASMVLIDSKNPLRELSPKLKQLIFHKKLGSFSEKVQRVRALAKFI
CHHHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
AFWVPNASIIKVEEAAKLAKLDLLTTFVRDFPKLAGFMGSHCLSLCGETDVEIIDAVRDH
HHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
YLPVDAADICPTAPVTVTLALADKIDTLVGLISVKEKVTSTRDPLGIRRAAIGVLRIIIE
CCCCCHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
NKLDVPLQVFLMKAIDLYPSSLFFSLIKKIGASSDEEKQRKDDVVNFTLSFFSERLKALL
CCCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FKSGVSQAVFASVSSGVTRPLVLTEKCIAISEWLKQAEGAEFMRLHKRMMGILKGFSGSA
HHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
KAKFSKKVLVLPEEVSLVQEFEGFQSLFCNAIKERNFEMAFSRCADLLKPITVFMDSVLV
CHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHC
NVEDPELKKNRIGLLLSISETLNELCNFDVLSRNFS
CCCCCCCCCCCCCEEEEHHHHHHHHHCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA