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The map label for this gene is pheS

Identifier: 88608710

GI number: 88608710

Start: 182149

End: 183126

Strand: Direct

Name: pheS

Synonym: NSE_0216

Alternate gene names: 88608710

Gene position: 182149-183126 (Clockwise)

Preceding gene: 88608583

Following gene: 88608285

Centisome position: 21.2

GC content: 45.19

Gene sequence:

>978_bases
GTGACTGTTAATTATGAGAAACTCAGAAGGCGTTTTTATTTACGTCTTGACGCTGTGTTTTCTGTTGAGGAGCTTAACAA
GCTTCGGTTTGAGTACTTGTCTAGCAAGAGTGGCCTGGTTAGAGGTGCTTTGGCTGCCCTCATCCAGCAGGGTGTGGAAG
CGGAACAACTCAGCATGTGGACTGGTCTACTGAAGGAGTGCAATCTGGCTCTAGCACGCAGGTTGGAGTTTATACGTGAA
AGGGAGTCAAATGAAAAACTTACTGCTGAGGCAGTAGATGTGTCCTATCCTGCCAGGCCTTTTAAAGTGGGGTTGAAGCA
TCCTCTTGTCAGAGTTATCGAGGATACTAGAAAAATTCTCACCTCACTCGGATTGGAACATGTAGACGGTCCGGAAGTAG
AAGATGAGTATCACGTATTTGATGCTTTAAATACGCCTGCACATCACCCGTCACGACAAATGCAGGATAGCTTCTACCTT
TCTGAGGTAGGTAAGCTTTTACGTACGCATACGTCTTCTGTACAGATTCGAGCAATGGAGGAGCGCGAGCCGCCCTTTTA
TATTTTTTCGCTGGGCAAAGTCTATCGGAATGATTGGGATGCTACACATACCCCTATGTTCCATCAGGTGGAAGTTCTAT
GTGTCGACGTGGATATAAATATGTCACATATGCGATATTGTGTTGGTTTCTTTCTGGAAAATCTCTTTGGATGCGCCAAA
ATGAGAATGAGACCGAGTTACTTTCCTTTTACCGAGCCCTCCGCTGAGATCGATATAAGGCGCCAAGATGGAAAATGGCT
AGAAGTGATGGGGTGTGGTATGGTACATCCAAATGTTTTGCAGAACGTTGGTATATCGCCTGATAAGTATAGAGGTTTTG
CTTTTGGTGCTGGGTTGGAAAGGCTGGCGGTACTGCGTTATGATATTCATGACTTGCGAAGCCTTTATTCAAACGATTTG
AGGTGGAAGGTAAGCTGA

Upstream 100 bases:

>100_bases
AAGCTGCTGGGGTGGATGTGAACAGAAAAGTACTTTCTGAGCTTGCTATAAGCCAGCCAAGTGCTTTTGGTGAAATAGTA
AACAAAGCCAAAGCTGCGCT

Downstream 100 bases:

>100_bases
TCGTGGTGTTTTTACTGTAAGGTGATTTTGCTTGAACGTGAGGGTTTCCATAGGTGCTGATCATGCGGGGTTTGCACTCA
AAGCAGTTTTGATAGACTTC

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MTVNYEKLRRRFYLRLDAVFSVEELNKLRFEYLSSKSGLVRGALAALIQQGVEAEQLSMWTGLLKECNLALARRLEFIRE
RESNEKLTAEAVDVSYPARPFKVGLKHPLVRVIEDTRKILTSLGLEHVDGPEVEDEYHVFDALNTPAHHPSRQMQDSFYL
SEVGKLLRTHTSSVQIRAMEEREPPFYIFSLGKVYRNDWDATHTPMFHQVEVLCVDVDINMSHMRYCVGFFLENLFGCAK
MRMRPSYFPFTEPSAEIDIRRQDGKWLEVMGCGMVHPNVLQNVGISPDKYRGFAFGAGLERLAVLRYDIHDLRSLYSNDL
RWKVS

Sequences:

>Translated_325_residues
MTVNYEKLRRRFYLRLDAVFSVEELNKLRFEYLSSKSGLVRGALAALIQQGVEAEQLSMWTGLLKECNLALARRLEFIRE
RESNEKLTAEAVDVSYPARPFKVGLKHPLVRVIEDTRKILTSLGLEHVDGPEVEDEYHVFDALNTPAHHPSRQMQDSFYL
SEVGKLLRTHTSSVQIRAMEEREPPFYIFSLGKVYRNDWDATHTPMFHQVEVLCVDVDINMSHMRYCVGFFLENLFGCAK
MRMRPSYFPFTEPSAEIDIRRQDGKWLEVMGCGMVHPNVLQNVGISPDKYRGFAFGAGLERLAVLRYDIHDLRSLYSNDL
RWKVS
>Mature_324_residues
TVNYEKLRRRFYLRLDAVFSVEELNKLRFEYLSSKSGLVRGALAALIQQGVEAEQLSMWTGLLKECNLALARRLEFIRER
ESNEKLTAEAVDVSYPARPFKVGLKHPLVRVIEDTRKILTSLGLEHVDGPEVEDEYHVFDALNTPAHHPSRQMQDSFYLS
EVGKLLRTHTSSVQIRAMEEREPPFYIFSLGKVYRNDWDATHTPMFHQVEVLCVDVDINMSHMRYCVGFFLENLFGCAKM
RMRPSYFPFTEPSAEIDIRRQDGKWLEVMGCGMVHPNVLQNVGISPDKYRGFAFGAGLERLAVLRYDIHDLRSLYSNDLR
WKVS

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=284, Percent_Identity=28.8732394366197, Blast_Score=114, Evalue=8e-26,
Organism=Homo sapiens, GI5729820, Length=224, Percent_Identity=33.0357142857143, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1788007, Length=315, Percent_Identity=46.6666666666667, Blast_Score=288, Evalue=4e-79,
Organism=Caenorhabditis elegans, GI32563657, Length=268, Percent_Identity=29.8507462686567, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17508957, Length=268, Percent_Identity=29.8507462686567, Blast_Score=118, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI32566635, Length=261, Percent_Identity=32.5670498084291, Blast_Score=103, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6321087, Length=258, Percent_Identity=31.0077519379845, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6325304, Length=263, Percent_Identity=27.3764258555133, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI18858079, Length=278, Percent_Identity=29.4964028776978, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI17137424, Length=230, Percent_Identity=33.0434782608696, Blast_Score=105, Evalue=6e-23,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 37659; Mature: 37528

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVNYEKLRRRFYLRLDAVFSVEELNKLRFEYLSSKSGLVRGALAALIQQGVEAEQLSMW
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHH
TGLLKECNLALARRLEFIRERESNEKLTAEAVDVSYPARPFKVGLKHPLVRVIEDTRKIL
HHHHHHHHHHHHHHHHHHHHCCCCCCEEHHEECCCCCCCHHHHCHHHHHHHHHHHHHHHH
TSLGLEHVDGPEVEDEYHVFDALNTPAHHPSRQMQDSFYLSEVGKLLRTHTSSVQIRAME
HHCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
EREPPFYIFSLGKVYRNDWDATHTPMFHQVEVLCVDVDINMSHMRYCVGFFLENLFGCAK
CCCCCEEEEEHHHHHHCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHH
MRMRPSYFPFTEPSAEIDIRRQDGKWLEVMGCGMVHPNVLQNVGISPDKYRGFAFGAGLE
HCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCHHHHHHCCCCCCCCCCEEHHCCHH
RLAVLRYDIHDLRSLYSNDLRWKVS
HHHHHHHHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure 
TVNYEKLRRRFYLRLDAVFSVEELNKLRFEYLSSKSGLVRGALAALIQQGVEAEQLSMW
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHH
TGLLKECNLALARRLEFIRERESNEKLTAEAVDVSYPARPFKVGLKHPLVRVIEDTRKIL
HHHHHHHHHHHHHHHHHHHHCCCCCCEEHHEECCCCCCCHHHHCHHHHHHHHHHHHHHHH
TSLGLEHVDGPEVEDEYHVFDALNTPAHHPSRQMQDSFYLSEVGKLLRTHTSSVQIRAME
HHCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
EREPPFYIFSLGKVYRNDWDATHTPMFHQVEVLCVDVDINMSHMRYCVGFFLENLFGCAK
CCCCCEEEEEHHHHHHCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHH
MRMRPSYFPFTEPSAEIDIRRQDGKWLEVMGCGMVHPNVLQNVGISPDKYRGFAFGAGLE
HCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCHHHHHHCCCCCCCCCCEEHHCCHH
RLAVLRYDIHDLRSLYSNDLRWKVS
HHHHHHHHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA