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The map label for this gene is ispF

Identifier: 88608695

GI number: 88608695

Start: 110751

End: 111257

Strand: Reverse

Name: ispF

Synonym: NSE_0134

Alternate gene names: 88608695

Gene position: 111257-110751 (Counterclockwise)

Preceding gene: 88608866

Following gene: 88608505

Centisome position: 12.95

GC content: 42.8

Gene sequence:

>507_bases
ATGTACCGTGTTGGATTCGGATACGATGTCCACAAGATAGACAAACCTGTAAAAACTGGCAATACCTGTATTGTCTTGTG
CGGAGTGTCTGTGCCCTGCGAGTACAAAGTCATTGCACACTCAGATGGTGATGTTGCGCTGCATGCATTGGTTGACGCAC
TGCTTGGCGCAGCATGTATAGAAGGTGCAAATGATATAGGCATGCTTTTTCCTGATACAGACGAAAAATGGAGGGGAAAG
TCATCATCTTACTTTGTAGAACATGTAGTAACGCTCATTCAAAGAGCTGGGTACGTGGTAAATAACGCAGATCTTACAAT
AGTGTGTGAAACACCGAGAATTCAGCAATACAAAAGCGAGATGCAGCAACAGGTAGCAGACTTACTTTGTGTTAGGAAAG
AGCAGATCAATGTAAAAGCAACAACAACCGAAAAACTAGGCTTTGAAGGACGTAAAGAAGGAATCGCAGCATACGCGATT
GTTTCACTGACTAAAATAAGACCATAG

Upstream 100 bases:

>100_bases
AAATCGGGTGCTATTGAGGATAAGAACAAAAGAAAACATTTTCTTTTTCTAAGATTCGTGTTAGGAGTCTGTCGCTCGTG
ATACATCATTCGGCCTTGCT

Downstream 100 bases:

>100_bases
CTTTTCAAAGAGTAATCAGTAAGACAGTTCATACAAAAAAACGTCCATGAGTGGAGATAAAGTTCAGTTGCGCGAGAAAT
ACCGCAAAATAAGAAAAAGC

Product: 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MYRVGFGYDVHKIDKPVKTGNTCIVLCGVSVPCEYKVIAHSDGDVALHALVDALLGAACIEGANDIGMLFPDTDEKWRGK
SSSYFVEHVVTLIQRAGYVVNNADLTIVCETPRIQQYKSEMQQQVADLLCVRKEQINVKATTTEKLGFEGRKEGIAAYAI
VSLTKIRP

Sequences:

>Translated_168_residues
MYRVGFGYDVHKIDKPVKTGNTCIVLCGVSVPCEYKVIAHSDGDVALHALVDALLGAACIEGANDIGMLFPDTDEKWRGK
SSSYFVEHVVTLIQRAGYVVNNADLTIVCETPRIQQYKSEMQQQVADLLCVRKEQINVKATTTEKLGFEGRKEGIAAYAI
VSLTKIRP
>Mature_168_residues
MYRVGFGYDVHKIDKPVKTGNTCIVLCGVSVPCEYKVIAHSDGDVALHALVDALLGAACIEGANDIGMLFPDTDEKWRGK
SSSYFVEHVVTLIQRAGYVVNNADLTIVCETPRIQQYKSEMQQQVADLLCVRKEQINVKATTTEKLGFEGRKEGIAAYAI
VSLTKIRP

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=163, Percent_Identity=44.1717791411043, Blast_Score=132, Evalue=9e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_NEOSM (Q2GER2)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506034.1
- ProteinModelPortal:   Q2GER2
- SMR:   Q2GER2
- STRING:   Q2GER2
- GeneID:   3931795
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0134
- TIGR:   NSE_0134
- eggNOG:   COG0245
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- PhylomeDB:   Q2GER2
- ProtClustDB:   CLSK2528087
- BioCyc:   NSEN222891:NSE_0134-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 18480; Mature: 18480

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRVGFGYDVHKIDKPVKTGNTCIVLCGVSVPCEYKVIAHSDGDVALHALVDALLGAACI
CEEECCCCCHHHHCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
EGANDIGMLFPDTDEKWRGKSSSYFVEHVVTLIQRAGYVVNNADLTIVCETPRIQQYKSE
CCCCCCEEEECCCCHHHCCCCCCHHHHHHHHHHHHCCEEEECCCEEEEECCCCHHHHHHH
MQQQVADLLCVRKEQINVKATTTEKLGFEGRKEGIAAYAIVSLTKIRP
HHHHHHHHHHHHHHHCEEEEECHHHCCCCCCCCCCHHHHEEEHHHCCC
>Mature Secondary Structure
MYRVGFGYDVHKIDKPVKTGNTCIVLCGVSVPCEYKVIAHSDGDVALHALVDALLGAACI
CEEECCCCCHHHHCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
EGANDIGMLFPDTDEKWRGKSSSYFVEHVVTLIQRAGYVVNNADLTIVCETPRIQQYKSE
CCCCCCEEEECCCCHHHCCCCCCHHHHHHHHHHHHCCEEEECCCEEEEECCCCHHHHHHH
MQQQVADLLCVRKEQINVKATTTEKLGFEGRKEGIAAYAIVSLTKIRP
HHHHHHHHHHHHHHHCEEEEECHHHCCCCCCCCCCHHHHEEEHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA