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The map label for this gene is pleD

Identifier: 88608688

GI number: 88608688

Start: 433470

End: 434840

Strand: Reverse

Name: pleD

Synonym: NSE_0512

Alternate gene names: 88608688

Gene position: 434840-433470 (Counterclockwise)

Preceding gene: 88608770

Following gene: 88608081

Centisome position: 50.62

GC content: 37.86

Gene sequence:

>1371_bases
ATGACAGCATTAATTCTAGTTGTGGATGATATTCCGAGCAATATTAAGATATTAGAGATTAAGTTGACAGCAAAGTACTA
TCAAGTGATTACTGCTTCTTCGGGGATTGCTGCGATAGAGCTTGCTAAGCAGCACCAGCCAGATGTAATCTTGCTGGATG
TAATGATGCCGGAAATGGATGGATTTGAAACGTGTAGACGCTTGAAAAGTGACTATGCCACTATGCATATTCCAGTGATT
ATTGTTACCGCGCTCAATGATATTGAAAACAAAGTGGAAGGTCTAAATAGTGGAGCTGATGATTTTCTTGTTAAACCAGC
AAAAGACTTTACTCTCTTCATAAGAATAAAGTCTCTATTACGGTTCAAAACTGTTCTGGATGAGGTACGTCTCAGAATAA
GCACTAAGTCTCAAATTACCAGCGGTACGGAAAGTAAAATTATAAATTATGTGGAAGATATCTCATCTGGAGTGATTGCA
ATAGTTAATCAACAGTATTCTGAATCCAAAGAGCTAGAGGAAATACTTAAGTCAACATTTCCGCACGTACTTTATCTTAC
TGAAAAAGAAGCAGAATATGCGTCTTTTGACGTCCTGATAGTAAATATCAGTCTCTCTAAGGATCTGCTTAGACTTTGTT
CTGCGATCAAAAGCCACCTCAAGACAAGGTCCATACCGATTATGGCTATAGTTGATGATGAAGACGATGAAAGTTTAATC
ACTGAGATAACGGAACTTGGAATAAATGATTATATATGCGTTCCGATTAATAAAAGCGAGTTCATTGCTAGAGTAACTAC
GCAGGTCAAGCGGAAAAAATACCAAGATATAATCGAAAAAAATGTGGAAGACAGTCTTAAGATGGCCGTCATAGACCCGC
TCACATCGTTGTACAACAGATACTATTTTGAAAAGTACATGGATGAGCTTTTAGCGACCTTAACGAAGTCACAAAATAAG
ACCTTTTCCCTAATGATGATAGATATTGATTACTTTAAAAAAATCAATGATGTTTGTGGACATCTTTCTGGTGACCTTGT
TTTAAAACAACTTGCTGCATGTTTGAAAGGTTGTCTCAGGCTTAATGACTTGGTGGCTAAATTTGGAGGAGAGGAAATAG
TTGTTGTTCTTGTCGATGTCGGGCTTGAGGATGCTTTTGATACCGCTGAACGAATTAGAAAAACTGTTGAGAAAACAGAA
TTCAGCACTATCAATGATGAAGTGAAAGTTCCGTTGACTGTTAGTATAGGAGTGTCCGAATATCAACCTCAGGATACTAC
GAAAACCTTAATAGGTAGAGCTGATGCAAACCTCTACATCGCAAAGGAGAAGGGTAGAAATCGGGTGGTAATGTCTGATC
ATTGTCACTAG

Upstream 100 bases:

>100_bases
GTAGAAGAGAAAGATGCAAAGAAAACTGAAGAAAATGGCCAGTGAATAATAGTGCAGTGGTGTATATCGTTTTGTTACCT
AAATATGAGCCTGGAAAACA

Downstream 100 bases:

>100_bases
TGAATCTGTTTTATATCCCATCAATGCCTCTGAATAGCTAGATTTTCTTGACATTAAAGTGGTGCTTTAGTCTCCGAGTG
TTTGCGTACATAAAAATAGG

Product: response regulator PleD

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MTALILVVDDIPSNIKILEIKLTAKYYQVITASSGIAAIELAKQHQPDVILLDVMMPEMDGFETCRRLKSDYATMHIPVI
IVTALNDIENKVEGLNSGADDFLVKPAKDFTLFIRIKSLLRFKTVLDEVRLRISTKSQITSGTESKIINYVEDISSGVIA
IVNQQYSESKELEEILKSTFPHVLYLTEKEAEYASFDVLIVNISLSKDLLRLCSAIKSHLKTRSIPIMAIVDDEDDESLI
TEITELGINDYICVPINKSEFIARVTTQVKRKKYQDIIEKNVEDSLKMAVIDPLTSLYNRYYFEKYMDELLATLTKSQNK
TFSLMMIDIDYFKKINDVCGHLSGDLVLKQLAACLKGCLRLNDLVAKFGGEEIVVVLVDVGLEDAFDTAERIRKTVEKTE
FSTINDEVKVPLTVSIGVSEYQPQDTTKTLIGRADANLYIAKEKGRNRVVMSDHCH

Sequences:

>Translated_456_residues
MTALILVVDDIPSNIKILEIKLTAKYYQVITASSGIAAIELAKQHQPDVILLDVMMPEMDGFETCRRLKSDYATMHIPVI
IVTALNDIENKVEGLNSGADDFLVKPAKDFTLFIRIKSLLRFKTVLDEVRLRISTKSQITSGTESKIINYVEDISSGVIA
IVNQQYSESKELEEILKSTFPHVLYLTEKEAEYASFDVLIVNISLSKDLLRLCSAIKSHLKTRSIPIMAIVDDEDDESLI
TEITELGINDYICVPINKSEFIARVTTQVKRKKYQDIIEKNVEDSLKMAVIDPLTSLYNRYYFEKYMDELLATLTKSQNK
TFSLMMIDIDYFKKINDVCGHLSGDLVLKQLAACLKGCLRLNDLVAKFGGEEIVVVLVDVGLEDAFDTAERIRKTVEKTE
FSTINDEVKVPLTVSIGVSEYQPQDTTKTLIGRADANLYIAKEKGRNRVVMSDHCH
>Mature_455_residues
TALILVVDDIPSNIKILEIKLTAKYYQVITASSGIAAIELAKQHQPDVILLDVMMPEMDGFETCRRLKSDYATMHIPVII
VTALNDIENKVEGLNSGADDFLVKPAKDFTLFIRIKSLLRFKTVLDEVRLRISTKSQITSGTESKIINYVEDISSGVIAI
VNQQYSESKELEEILKSTFPHVLYLTEKEAEYASFDVLIVNISLSKDLLRLCSAIKSHLKTRSIPIMAIVDDEDDESLIT
EITELGINDYICVPINKSEFIARVTTQVKRKKYQDIIEKNVEDSLKMAVIDPLTSLYNRYYFEKYMDELLATLTKSQNKT
FSLMMIDIDYFKKINDVCGHLSGDLVLKQLAACLKGCLRLNDLVAKFGGEEIVVVLVDVGLEDAFDTAERIRKTVEKTEF
STINDEVKVPLTVSIGVSEYQPQDTTKTLIGRADANLYIAKEKGRNRVVMSDHCH

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=165, Percent_Identity=41.8181818181818, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI145693134, Length=159, Percent_Identity=37.1069182389937, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI87082007, Length=178, Percent_Identity=38.2022471910112, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI87081881, Length=181, Percent_Identity=39.7790055248619, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1787816, Length=163, Percent_Identity=34.3558282208589, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786584, Length=162, Percent_Identity=32.0987654320988, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787802, Length=210, Percent_Identity=33.8095238095238, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788381, Length=180, Percent_Identity=32.7777777777778, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788085, Length=177, Percent_Identity=35.0282485875706, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1786784, Length=117, Percent_Identity=37.6068376068376, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1786599, Length=121, Percent_Identity=34.7107438016529, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1790437, Length=102, Percent_Identity=43.1372549019608, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787541, Length=171, Percent_Identity=29.8245614035088, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788550, Length=132, Percent_Identity=32.5757575757576, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87081974, Length=175, Percent_Identity=28, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788394, Length=122, Percent_Identity=36.0655737704918, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1789809, Length=117, Percent_Identity=36.7521367521368, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788956, Length=165, Percent_Identity=31.5151515151515, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI145693157, Length=103, Percent_Identity=39.8058252427184, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1789149, Length=119, Percent_Identity=36.1344537815126, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87082012, Length=117, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790860, Length=100, Percent_Identity=33, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787229, Length=120, Percent_Identity=31.6666666666667, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI48994928, Length=102, Percent_Identity=34.3137254901961, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787375, Length=122, Percent_Identity=29.5081967213115, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 51402; Mature: 51271

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALILVVDDIPSNIKILEIKLTAKYYQVITASSGIAAIELAKQHQPDVILLDVMMPEMD
CEEEEEEEECCCCCEEEEEEEEEEEEEEEEECCCCCCEEHHHHCCCCCEEEEEEECCCCC
GFETCRRLKSDYATMHIPVIIVTALNDIENKVEGLNSGADDFLVKPAKDFTLFIRIKSLL
CHHHHHHHHHCCCEEEEEEEEEEHHHHHHHHHHHCCCCCCCCEECCCCCCEEHHHHHHHH
RFKTVLDEVRLRISTKSQITSGTESKIINYVEDISSGVIAIVNQQYSESKELEEILKSTF
HHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHCCCEEEEECCHHCCHHHHHHHHHHCC
PHVLYLTEKEAEYASFDVLIVNISLSKDLLRLCSAIKSHLKTRSIPIMAIVDDEDDESLI
CEEEEEECCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHH
TEITELGINDYICVPINKSEFIARVTTQVKRKKYQDIIEKNVEDSLKMAVIDPLTSLYNR
HHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
YYFEKYMDELLATLTKSQNKTFSLMMIDIDYFKKINDVCGHLSGDLVLKQLAACLKGCLR
HHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LNDLVAKFGGEEIVVVLVDVGLEDAFDTAERIRKTVEKTEFSTINDEVKVPLTVSIGVSE
HHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCC
YQPQDTTKTLIGRADANLYIAKEKGRNRVVMSDHCH
CCCCCHHHHHHCCCCCCEEEEECCCCCEEEECCCCC
>Mature Secondary Structure 
TALILVVDDIPSNIKILEIKLTAKYYQVITASSGIAAIELAKQHQPDVILLDVMMPEMD
EEEEEEEECCCCCEEEEEEEEEEEEEEEEECCCCCCEEHHHHCCCCCEEEEEEECCCCC
GFETCRRLKSDYATMHIPVIIVTALNDIENKVEGLNSGADDFLVKPAKDFTLFIRIKSLL
CHHHHHHHHHCCCEEEEEEEEEEHHHHHHHHHHHCCCCCCCCEECCCCCCEEHHHHHHHH
RFKTVLDEVRLRISTKSQITSGTESKIINYVEDISSGVIAIVNQQYSESKELEEILKSTF
HHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHCCCEEEEECCHHCCHHHHHHHHHHCC
PHVLYLTEKEAEYASFDVLIVNISLSKDLLRLCSAIKSHLKTRSIPIMAIVDDEDDESLI
CEEEEEECCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHH
TEITELGINDYICVPINKSEFIARVTTQVKRKKYQDIIEKNVEDSLKMAVIDPLTSLYNR
HHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
YYFEKYMDELLATLTKSQNKTFSLMMIDIDYFKKINDVCGHLSGDLVLKQLAACLKGCLR
HHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LNDLVAKFGGEEIVVVLVDVGLEDAFDTAERIRKTVEKTEFSTINDEVKVPLTVSIGVSE
HHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCC
YQPQDTTKTLIGRADANLYIAKEKGRNRVVMSDHCH
CCCCCHHHHHHCCCCCCEEEEECCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA