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The map label for this gene is gltX-2

Identifier: 88608680

GI number: 88608680

Start: 656477

End: 657706

Strand: Reverse

Name: gltX-2

Synonym: NSE_0737

Alternate gene names: 88608680

Gene position: 657706-656477 (Counterclockwise)

Preceding gene: 88608162

Following gene: 88608886

Centisome position: 76.57

GC content: 40.16

Gene sequence:

>1230_bases
ATGGACGTTGTTACAAGGTTTGCGCCATCTCCGACAGGTAATTTGCACATTGGTGGTCTCAGGACTGCCATATTTAATTT
TCTCTATGCTAGAAGGCATGGTGGTAAGTTCCTTCTTAGAATTGAGGATACTGATTTAGCTAGGTCGAATACGGCTTATC
TTGAGTCAATTCTGCAGGCTCTTAAATGGCTTTCGCTTTCTTATGATGAGCTTGTGTACCAATCTGTCAATGTTAATAGG
CATAGAGAAGTTGCACTTGAGCTTTTGAACCGGGGTAAGGCTTATCACGATAATGGTGCAGTGCGCCTTAAAGTAAATCA
CGATGAGACAGTAAAATTTTCCGATTTGATTTTAGGCGAGATTTCAGCTGATGGTGATAGCATCAATGATGTCGTGATTC
TCAGGTCAGATGGGAGCCCAACCTATATGCTTGCAGTGGTTGTCGATGATCATGATATGGGTATAACTCACATAATAAGG
GGTTCTGATCATATTACGAATACTTTTGCCCAAAAACTAATATATGAAGGCATGGGTTGGTCTTTACCGGACTTTGCTCA
TATACCGCTAATACACAATACACATGGTGCTAAGCTCTCAAAGAGGGACGGTGCTGTCGACGTAATAGATTATAGAACTG
CAGGTATACTTCCTGAAGCGATGTTCAATTATTTGCTTAGGCTAGGATGGAGTCATGGTGATAAAGAAATATTCTCCATA
CAGGAGGCTATAGACCTTTTCGATATCACAGCAGTTGGCAAATCTCCTGCTCGCTTTGACCGTGAAAAGCTTTACAGCCT
TAATTCTTATTATATCAGTGGATTACCACTTGAGAGGATAGCCTCAGAGGTTCGGAGTCTTGTTGAAGAAAAAATAATAG
TTTCTTATAGTGCGTTCATTGCATTTGTTGAACTGTTTAGAAAAAAGTGTTCCTCAATTGTTGAATTGAAAGATAATCTT
CAGTTTTGTTGGTGTACTGATTCGTGTCTAGAGGTAGATAGGCTCTTGCTGCAGGAGGTACTAAAACTACTGGAAAATAC
CGATTGGGGTCCTAGTTTACAGCAAATTTTGAAAGAGTACATTTCTCTTTCTGGGTTTTCTGCTAAAGAGGTCTATACGA
ATCTGAGAATGGCCTTAATAGGGCAAGAACACTCCCCTTCGGTGGTGGAAATTATGCAAATCTTTGGTAAAGACGTTGTT
CTTAAGAAGTTGCGTAACTCTCTCAAATGA

Upstream 100 bases:

>100_bases
GGTAGGACTTACTATCATAGTGGGGGGTCCATGAGTCTTTACCTTGTCTAGTGGTAGGTCGAGTGTTAATGTTTTCAGGG
TGATTTAACATATTTCGTGA

Downstream 100 bases:

>100_bases
GTTTTCCATCGATAAATGTCTGTGTGTAAATTATCCTTTTTGGAAATTGTGGTCTACTCAGGAATAGTTGGTTGGGGTGT
GCTTCTCAGGGAGAATTGCT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 2; GluRS 2

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MDVVTRFAPSPTGNLHIGGLRTAIFNFLYARRHGGKFLLRIEDTDLARSNTAYLESILQALKWLSLSYDELVYQSVNVNR
HREVALELLNRGKAYHDNGAVRLKVNHDETVKFSDLILGEISADGDSINDVVILRSDGSPTYMLAVVVDDHDMGITHIIR
GSDHITNTFAQKLIYEGMGWSLPDFAHIPLIHNTHGAKLSKRDGAVDVIDYRTAGILPEAMFNYLLRLGWSHGDKEIFSI
QEAIDLFDITAVGKSPARFDREKLYSLNSYYISGLPLERIASEVRSLVEEKIIVSYSAFIAFVELFRKKCSSIVELKDNL
QFCWCTDSCLEVDRLLLQEVLKLLENTDWGPSLQQILKEYISLSGFSAKEVYTNLRMALIGQEHSPSVVEIMQIFGKDVV
LKKLRNSLK

Sequences:

>Translated_409_residues
MDVVTRFAPSPTGNLHIGGLRTAIFNFLYARRHGGKFLLRIEDTDLARSNTAYLESILQALKWLSLSYDELVYQSVNVNR
HREVALELLNRGKAYHDNGAVRLKVNHDETVKFSDLILGEISADGDSINDVVILRSDGSPTYMLAVVVDDHDMGITHIIR
GSDHITNTFAQKLIYEGMGWSLPDFAHIPLIHNTHGAKLSKRDGAVDVIDYRTAGILPEAMFNYLLRLGWSHGDKEIFSI
QEAIDLFDITAVGKSPARFDREKLYSLNSYYISGLPLERIASEVRSLVEEKIIVSYSAFIAFVELFRKKCSSIVELKDNL
QFCWCTDSCLEVDRLLLQEVLKLLENTDWGPSLQQILKEYISLSGFSAKEVYTNLRMALIGQEHSPSVVEIMQIFGKDVV
LKKLRNSLK
>Mature_409_residues
MDVVTRFAPSPTGNLHIGGLRTAIFNFLYARRHGGKFLLRIEDTDLARSNTAYLESILQALKWLSLSYDELVYQSVNVNR
HREVALELLNRGKAYHDNGAVRLKVNHDETVKFSDLILGEISADGDSINDVVILRSDGSPTYMLAVVVDDHDMGITHIIR
GSDHITNTFAQKLIYEGMGWSLPDFAHIPLIHNTHGAKLSKRDGAVDVIDYRTAGILPEAMFNYLLRLGWSHGDKEIFSI
QEAIDLFDITAVGKSPARFDREKLYSLNSYYISGLPLERIASEVRSLVEEKIIVSYSAFIAFVELFRKKCSSIVELKDNL
QFCWCTDSCLEVDRLLLQEVLKLLENTDWGPSLQQILKEYISLSGFSAKEVYTNLRMALIGQEHSPSVVEIMQIFGKDVV
LKKLRNSLK

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=486, Percent_Identity=29.6296296296296, Blast_Score=189, Evalue=5e-48,
Organism=Escherichia coli, GI1788743, Length=461, Percent_Identity=35.5748373101952, Blast_Score=282, Evalue=3e-77,
Organism=Escherichia coli, GI226510925, Length=284, Percent_Identity=35.2112676056338, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1786895, Length=96, Percent_Identity=37.5, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71993394, Length=478, Percent_Identity=28.0334728033473, Blast_Score=167, Evalue=8e-42,
Organism=Saccharomyces cerevisiae, GI6324540, Length=492, Percent_Identity=29.4715447154472, Blast_Score=196, Evalue=8e-51,
Organism=Saccharomyces cerevisiae, GI6321192, Length=97, Percent_Identity=34.020618556701, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324742, Length=93, Percent_Identity=37.6344086021505, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24665321, Length=485, Percent_Identity=31.7525773195876, Blast_Score=212, Evalue=3e-55,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE2_NEOSM (Q2GD33)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506613.1
- ProteinModelPortal:   Q2GD33
- SMR:   Q2GD33
- STRING:   Q2GD33
- GeneID:   3931573
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0737
- TIGR:   NSE_0737
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   CSSIVEL
- PhylomeDB:   Q2GD33
- ProtClustDB:   PRK01406
- BioCyc:   NSEN222891:NSE_0737-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 46230; Mature: 46230

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 201-201

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVVTRFAPSPTGNLHIGGLRTAIFNFLYARRHGGKFLLRIEDTDLARSNTAYLESILQA
CCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHH
LKWLSLSYDELVYQSVNVNRHREVALELLNRGKAYHDNGAVRLKVNHDETVKFSDLILGE
HHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEHHHHEEEE
ISADGDSINDVVILRSDGSPTYMLAVVVDDHDMGITHIIRGSDHITNTFAQKLIYEGMGW
ECCCCCCCCCEEEEECCCCCEEEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHCCCC
SLPDFAHIPLIHNTHGAKLSKRDGAVDVIDYRTAGILPEAMFNYLLRLGWSHGDKEIFSI
CCCCCCCCCEEECCCCCCCCCCCCCEEEEECCHHCCCHHHHHHHHHHHCCCCCCHHHHHH
QEAIDLFDITAVGKSPARFDREKLYSLNSYYISGLPLERIASEVRSLVEEKIIVSYSAFI
HHHHHHHHEEECCCCCHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
AFVELFRKKCSSIVELKDNLQFCWCTDSCLEVDRLLLQEVLKLLENTDWGPSLQQILKEY
HHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ISLSGFSAKEVYTNLRMALIGQEHSPSVVEIMQIFGKDVVLKKLRNSLK
HHCCCCCHHHHHHHHHHHEECCCCCCHHHHHHHHHCHHHHHHHHHHHCC
>Mature Secondary Structure
MDVVTRFAPSPTGNLHIGGLRTAIFNFLYARRHGGKFLLRIEDTDLARSNTAYLESILQA
CCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHH
LKWLSLSYDELVYQSVNVNRHREVALELLNRGKAYHDNGAVRLKVNHDETVKFSDLILGE
HHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEHHHHEEEE
ISADGDSINDVVILRSDGSPTYMLAVVVDDHDMGITHIIRGSDHITNTFAQKLIYEGMGW
ECCCCCCCCCEEEEECCCCCEEEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHCCCC
SLPDFAHIPLIHNTHGAKLSKRDGAVDVIDYRTAGILPEAMFNYLLRLGWSHGDKEIFSI
CCCCCCCCCEEECCCCCCCCCCCCCEEEEECCHHCCCHHHHHHHHHHHCCCCCCHHHHHH
QEAIDLFDITAVGKSPARFDREKLYSLNSYYISGLPLERIASEVRSLVEEKIIVSYSAFI
HHHHHHHHEEECCCCCHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
AFVELFRKKCSSIVELKDNLQFCWCTDSCLEVDRLLLQEVLKLLENTDWGPSLQQILKEY
HHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ISLSGFSAKEVYTNLRMALIGQEHSPSVVEIMQIFGKDVVLKKLRNSLK
HHCCCCCHHHHHHHHHHHEECCCCCCHHHHHHHHHCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA