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The map label for this gene is ribE-2

Identifier: 88608601

GI number: 88608601

Start: 755331

End: 755933

Strand: Direct

Name: ribE-2

Synonym: NSE_0853

Alternate gene names: 88608601

Gene position: 755331-755933 (Clockwise)

Preceding gene: 88608008

Following gene: 88608475

Centisome position: 87.93

GC content: 39.97

Gene sequence:

>603_bases
ATGTTTAAGGGAATAGTGCAAACACTAGGTTTAGTAACAAAGTTAATCGTGAGGGATAAACAAATTTTCCTTGCAGTTGA
TGCAAAAGAATTGGCACAGAGTGTGGAACTCGGTTCTTCCATAGCATGTAATGGTGTTTGTCTCACAGTAACTTCCATCA
CCAACACGGAATTAGAATTCTATGTCTCGCAAGAAACATTTAGCAGAACCAATTTCCATGCTCTTGAGAAAGGTTCGAAG
GTAAACTTGGAAACTTCTCTCAGGATCGGCGATACACTCGACGGACATTTTGTACAGGGCCATGTTGATGAAACTACTAT
GATTACTGCAATAGAAAAAACTGGTGAATCACACGTTTTCACGTTTGCCCTTACAGAAGGGATTTCACCGTTTATAACGT
ACAAGGGTAGCGTCTGCATCGACGGTACGTCTCTCACGGTTAATGAAGTATTTGAAAATGAGTTTAGTGTTAACATCATT
CCATATACTTGGAGCCAGACTATTTTTCACAGCAAGAAAGTTGGTGATAAGGTCAATGTGGAGAGCGATTTGTTAGCTCG
CTACTTAAAGCGATTGTTTGAGCATAGCAGACTTGTGATATAG

Upstream 100 bases:

>100_bases
TTGTACCTTTCACTTTGTAGTTAGAGTATAGTGTAAACAAACTTCCATTATGTACTCATAACAGAAATGCTTCTTATAGC
TGGTTTTGAACGACGAAGTT

Downstream 100 bases:

>100_bases
CACTGTTACATCTGTACGCGATGGTGCGGGATCGCCGCTTATACTTGATTTCTTATCTATTTAGGCTTACTAACTTCACT
CTGTAGTGACTCAACTCAGC

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MFKGIVQTLGLVTKLIVRDKQIFLAVDAKELAQSVELGSSIACNGVCLTVTSITNTELEFYVSQETFSRTNFHALEKGSK
VNLETSLRIGDTLDGHFVQGHVDETTMITAIEKTGESHVFTFALTEGISPFITYKGSVCIDGTSLTVNEVFENEFSVNII
PYTWSQTIFHSKKVGDKVNVESDLLARYLKRLFEHSRLVI

Sequences:

>Translated_200_residues
MFKGIVQTLGLVTKLIVRDKQIFLAVDAKELAQSVELGSSIACNGVCLTVTSITNTELEFYVSQETFSRTNFHALEKGSK
VNLETSLRIGDTLDGHFVQGHVDETTMITAIEKTGESHVFTFALTEGISPFITYKGSVCIDGTSLTVNEVFENEFSVNII
PYTWSQTIFHSKKVGDKVNVESDLLARYLKRLFEHSRLVI
>Mature_200_residues
MFKGIVQTLGLVTKLIVRDKQIFLAVDAKELAQSVELGSSIACNGVCLTVTSITNTELEFYVSQETFSRTNFHALEKGSK
VNLETSLRIGDTLDGHFVQGHVDETTMITAIEKTGESHVFTFALTEGISPFITYKGSVCIDGTSLTVNEVFENEFSVNII
PYTWSQTIFHSKKVGDKVNVESDLLARYLKRLFEHSRLVI

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=188, Percent_Identity=35.6382978723404, Blast_Score=105, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6319733, Length=203, Percent_Identity=34.9753694581281, Blast_Score=112, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 22247; Mature: 22247

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00693 LUM_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKGIVQTLGLVTKLIVRDKQIFLAVDAKELAQSVELGSSIACNGVCLTVTSITNTELEF
CCHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHCCCCEEECCEEEEEEEECCCEEEE
YVSQETFSRTNFHALEKGSKVNLETSLRIGDTLDGHFVQGHVDETTMITAIEKTGESHVF
EEEHHHHCCCCCHHHCCCCEECEEEEEEECCCCCCCEEECCCCCCEEEEEEECCCCCEEE
TFALTEGISPFITYKGSVCIDGTSLTVNEVFENEFSVNIIPYTWSQTIFHSKKVGDKVNV
EEEECCCCCEEEEECCCEEECCCCEEHHHHHCCCEEEEEEEEECCHHHHHHHHCCCCCCC
ESDLLARYLKRLFEHSRLVI
CHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFKGIVQTLGLVTKLIVRDKQIFLAVDAKELAQSVELGSSIACNGVCLTVTSITNTELEF
CCHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHCCCCEEECCEEEEEEEECCCEEEE
YVSQETFSRTNFHALEKGSKVNLETSLRIGDTLDGHFVQGHVDETTMITAIEKTGESHVF
EEEHHHHCCCCCHHHCCCCEECEEEEEEECCCCCCCEEECCCCCCEEEEEEECCCCCEEE
TFALTEGISPFITYKGSVCIDGTSLTVNEVFENEFSVNIIPYTWSQTIFHSKKVGDKVNV
EEEECCCCCEEEEECCCEEECCCCEEHHHHHCCCEEEEEEEEECCHHHHHHHHCCCCCCC
ESDLLARYLKRLFEHSRLVI
CHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]