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The map label for this gene is purB

Identifier: 88608580

GI number: 88608580

Start: 52474

End: 53772

Strand: Reverse

Name: purB

Synonym: NSE_0059

Alternate gene names: 88608580

Gene position: 53772-52474 (Counterclockwise)

Preceding gene: 88607969

Following gene: 88608422

Centisome position: 6.26

GC content: 42.88

Gene sequence:

>1299_bases
ATGATTCCCAGGTACAGCAGAGAGGTCGCTGCATCGATCTGGAGGGATGATACTAGGTTTTCCCTGTGGTTGGAGATTGA
GCTCCTTGCGTGTGAGGCACAAGCTCGGCTTGGTAATATACCTCAGGAAAGTTTAAGCGTCATTTTAGAAAAAGCAGCCT
TTGATATAGAAAGGATCGGGTTCATTGAAAAAGAGGTTAAGCACGATGTTATAGCTTTTTTAACAAGTGTGGCGGAGTTC
GTTGGCCCTGATGCAAGATATATACACATGGGCATGACTAGCTCAGACGTAGTAGATACTGCTTTTTCACTCCAGCTTCG
TCGTGCGGGAACTTTACTCTTAGAAGGCTTAGATAAAGTTACCGAAGCACTTAGGGAAAGGGCTCTCGAGCACAAGAACA
CTATCTGTGTTGCTAGGACACATGGAATTCATGCTGAACCTATTACTCTTGGGCTTAAGTTCGCTCGATTCTATCAGGAA
TTTAAACGAAATCGACTAAGACTTTCGCAGGTAATCAAAGAGATTTCTGTATGTAAGATTTCTGGTGCTGTAGGACAGTT
CGGTAATATTGACCCATTTGTCCAAGAGTACGTTGCAGAAAAGCTTGGACTTGAACCAGAACCACTGGCGACGCAAGTTA
TACCACGTGATAGGTACGCTGTTTTTTTCAGTACTCTTGCTCTTATTGCTTCCTCAATAGAGAACGTTGCAACAGAAATT
AGACATCTTCAAAGAAGTGAGGTGTTGGAATGTTCAGAGTACTTCTCGCCAGGTCAGAAAGGGAGCTCAGCGATGCCTCA
CAAGAAAAACCCTATTTTGAGCGAAAACCTTACTGGCTTAGCTCGCATAGTTAGGAGCACGGTCATCCCGTTTCTTGAAA
ATGTCTCTCTGTGGCACGAAAGGGATATCTCACATTCCTCAGTTGAAAGAGTGTGTGCTCCAATGGCCTGTGTCACGTTA
GACTTCGCATTGGAAAGACTTTCTACTGTAATAAGGGAATTAGTGGTCTTTGAGAAGAATATCGAGAAGAATCTGAACCT
TCTAGGTGGACTTATTTTCTCACAAAAGGTCCTTCTAGCTCTTATAGAATCTGGAAAGACGCGGGAAGAGGCTTACCATA
TAGTTCAGGAAAACGCGATGAAAAGTTGGAATGACACGAGCACGTCTTTCGTAGAGAACATAAAGATGGATAAACGTGTG
ACATTTCAGGAAGTTGATAAACTCTTTTGCTACGAGAGTTATCTCAGACATCTGGATTACATATTCGAGCATGTCTTTAG
CGCTAGTGAAGTAAGTTAA

Upstream 100 bases:

>100_bases
TACATTCCAGAGTTGAGTGTTCTCTTAAAATTCACATCTTAGATGCCTCTAGAAGGGGCGCTGATGGGACCATCACAGGC
ACGTCGTAATTTGACCAGAT

Downstream 100 bases:

>100_bases
TATATTAAATCTAAGATTTGTATGCGTTGTCACTGAACTTGTATAAACAATGATCAGGGTGTGCTGTGAGTTGATTTACC
CAAGCTGAAAGGTGCAAAAG

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MIPRYSREVAASIWRDDTRFSLWLEIELLACEAQARLGNIPQESLSVILEKAAFDIERIGFIEKEVKHDVIAFLTSVAEF
VGPDARYIHMGMTSSDVVDTAFSLQLRRAGTLLLEGLDKVTEALRERALEHKNTICVARTHGIHAEPITLGLKFARFYQE
FKRNRLRLSQVIKEISVCKISGAVGQFGNIDPFVQEYVAEKLGLEPEPLATQVIPRDRYAVFFSTLALIASSIENVATEI
RHLQRSEVLECSEYFSPGQKGSSAMPHKKNPILSENLTGLARIVRSTVIPFLENVSLWHERDISHSSVERVCAPMACVTL
DFALERLSTVIRELVVFEKNIEKNLNLLGGLIFSQKVLLALIESGKTREEAYHIVQENAMKSWNDTSTSFVENIKMDKRV
TFQEVDKLFCYESYLRHLDYIFEHVFSASEVS

Sequences:

>Translated_432_residues
MIPRYSREVAASIWRDDTRFSLWLEIELLACEAQARLGNIPQESLSVILEKAAFDIERIGFIEKEVKHDVIAFLTSVAEF
VGPDARYIHMGMTSSDVVDTAFSLQLRRAGTLLLEGLDKVTEALRERALEHKNTICVARTHGIHAEPITLGLKFARFYQE
FKRNRLRLSQVIKEISVCKISGAVGQFGNIDPFVQEYVAEKLGLEPEPLATQVIPRDRYAVFFSTLALIASSIENVATEI
RHLQRSEVLECSEYFSPGQKGSSAMPHKKNPILSENLTGLARIVRSTVIPFLENVSLWHERDISHSSVERVCAPMACVTL
DFALERLSTVIRELVVFEKNIEKNLNLLGGLIFSQKVLLALIESGKTREEAYHIVQENAMKSWNDTSTSFVENIKMDKRV
TFQEVDKLFCYESYLRHLDYIFEHVFSASEVS
>Mature_432_residues
MIPRYSREVAASIWRDDTRFSLWLEIELLACEAQARLGNIPQESLSVILEKAAFDIERIGFIEKEVKHDVIAFLTSVAEF
VGPDARYIHMGMTSSDVVDTAFSLQLRRAGTLLLEGLDKVTEALRERALEHKNTICVARTHGIHAEPITLGLKFARFYQE
FKRNRLRLSQVIKEISVCKISGAVGQFGNIDPFVQEYVAEKLGLEPEPLATQVIPRDRYAVFFSTLALIASSIENVATEI
RHLQRSEVLECSEYFSPGQKGSSAMPHKKNPILSENLTGLARIVRSTVIPFLENVSLWHERDISHSSVERVCAPMACVTL
DFALERLSTVIRELVVFEKNIEKNLNLLGGLIFSQKVLLALIESGKTREEAYHIVQENAMKSWNDTSTSFVENIKMDKRV
TFQEVDKLFCYESYLRHLDYIFEHVFSASEVS

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=417, Percent_Identity=27.0983213429257, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI183227688, Length=408, Percent_Identity=28.1862745098039, Blast_Score=125, Evalue=9e-29,
Organism=Escherichia coli, GI1787376, Length=319, Percent_Identity=27.8996865203762, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17508577, Length=389, Percent_Identity=26.9922879177378, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI32564234, Length=338, Percent_Identity=27.810650887574, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323391, Length=430, Percent_Identity=26.2790697674419, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24647570, Length=381, Percent_Identity=26.7716535433071, Blast_Score=99, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49032; Mature: 49032

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPRYSREVAASIWRDDTRFSLWLEIELLACEAQARLGNIPQESLSVILEKAAFDIERIG
CCCCHHHHHHHHHHCCCCCEEEEEEEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
FIEKEVKHDVIAFLTSVAEFVGPDARYIHMGMTSSDVVDTAFSLQLRRAGTLLLEGLDKV
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TEALRERALEHKNTICVARTHGIHAEPITLGLKFARFYQEFKRNRLRLSQVIKEISVCKI
HHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGQFGNIDPFVQEYVAEKLGLEPEPLATQVIPRDRYAVFFSTLALIASSIENVATEI
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RHLQRSEVLECSEYFSPGQKGSSAMPHKKNPILSENLTGLARIVRSTVIPFLENVSLWHE
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDISHSSVERVCAPMACVTLDFALERLSTVIRELVVFEKNIEKNLNLLGGLIFSQKVLLA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIESGKTREEAYHIVQENAMKSWNDTSTSFVENIKMDKRVTFQEVDKLFCYESYLRHLDY
HHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
IFEHVFSASEVS
HHHHHHHHCCCC
>Mature Secondary Structure
MIPRYSREVAASIWRDDTRFSLWLEIELLACEAQARLGNIPQESLSVILEKAAFDIERIG
CCCCHHHHHHHHHHCCCCCEEEEEEEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
FIEKEVKHDVIAFLTSVAEFVGPDARYIHMGMTSSDVVDTAFSLQLRRAGTLLLEGLDKV
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TEALRERALEHKNTICVARTHGIHAEPITLGLKFARFYQEFKRNRLRLSQVIKEISVCKI
HHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGQFGNIDPFVQEYVAEKLGLEPEPLATQVIPRDRYAVFFSTLALIASSIENVATEI
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RHLQRSEVLECSEYFSPGQKGSSAMPHKKNPILSENLTGLARIVRSTVIPFLENVSLWHE
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDISHSSVERVCAPMACVTLDFALERLSTVIRELVVFEKNIEKNLNLLGGLIFSQKVLLA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIESGKTREEAYHIVQENAMKSWNDTSTSFVENIKMDKRVTFQEVDKLFCYESYLRHLDY
HHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
IFEHVFSASEVS
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]