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The map label for this gene is clpA

Identifier: 88608467

GI number: 88608467

Start: 256575

End: 258797

Strand: Reverse

Name: clpA

Synonym: NSE_0313

Alternate gene names: 88608467

Gene position: 258797-256575 (Counterclockwise)

Preceding gene: 88608569

Following gene: 88608313

Centisome position: 30.13

GC content: 40.08

Gene sequence:

>2223_bases
ATGATCTCGCCTAGTTTAGAGAATACGCTTCAGAACGCGCTCGTTTTGGCACGGAGTTTTTCGCATTCTTACGTCACACT
GGAACATCTTCTTCTTGCCCTCACGGATGACGAAGAAGTTTCGAGAATCGCCTTCAACTCAAGCATGAATTTTGCCAAAC
TAAGGGATTCGCTTATTCATTTTCTTAGCACAGCAAAGTTTTTCGCACGCCTTTCCAATGAATGTGTCGATATAAGCAAA
ATCAAACCAACACTTGGATTAAGACGCGTCATACAGAGAAGCAAAATTCATGCAAACATAGCGGAATTTGGACAAATCAC
AGGTCTACATGTTTTGATCGAACTGTTCTGTGAACAAGATTCCTATGCATTCTCGTTTCTCACAGAAAGCAATATAACAC
TTACAGAGCTCATGTCTCACGTGATGAAGTCCCGTAAAGGGTCCCCAACTGGAGGTACATCTACTGCAAATACGGATGCA
CTCAGAAGTGCAGAACTTGATAATTTAAAGGAATACTGCACGGACATGAATGAGCTAGCTATTCAAGGAAGGATAGACAA
ACTCATTGATAGAAAAAAGGAAATTGAGAGAACAATAGAGATTCTTTTACGGAGAACCAAAAACAATCCTATTTACGTCG
GTGAACCTGGGGTTGGAAAGACCGCCATAGTAGAAGGCTTGGTACATGCGATAGTCAGTGGTAACGTCCCACAGAAGCTG
AGAAATACACGTGTGTATGCACTCAAGATAGGCTCTTTACTCGCAGGTACACGCTATAGGGGTGATTTTGAAGAGCGTAT
TAGAACAGTCATGAACGAAATATCCAGTAGTAAGAATACAATACTTTTTATAGATGAAATCCACACAATCGTCGGTGCTG
GCTCAACAAGTAATGGCTCTATTGATGCAGGAAATCTTCTAAAGCCATTTCTGACAAAGGGTCCTCTCAGGTGTATTGGC
GCTACGACCTATAGAGAATACTCCACACACTTTGAAAAAGATGCAGCACTTGATAGGAGATTTCAGCGGATAGAAATAGA
GGAACCATCTATAAGTGACACTCTAAATATAATTGACGGGACAAAGTCATATTACGAGGATTTTCACAGGGTGCATTATA
CGAAGGCATCCCTTAATGAGATAGTAAATCTAGCCCACAGATACATACCAAACAGAGCCTTCCCCGATAAAGCTATAGAT
TTAATGGATGATTTGGGTGCATCATACAATGCGGAGTTATTCGATATCAAAAAAAGGCGCGATAGAAAAATAGATATTAA
GTATGTCAGGGAAACGCTTGCAAAGCGTTTTAATATTCCGCTTGCACAGTTAAATTATACCGTGGAGGAAGTATTAACTT
CCCTTGAGAGAAACCTTAAGGCAGAAATTTTCGATCAAGACAATATCATAGAGCAGGTCATCTATTCGCTGAGACCTGGG
CTACTTGGATTAAAAGGTGAAAAACCTTTGGCTTCGTACCTATTTGGGGGTTCTACGGGTGTCGGCAAAACAGAGCTTGC
TAAACAAATTGCCAAAAACTTGTGCATGCGGTTTGTGAGAATTGATATGTCCGAATATATGGAAGGCCACGCTGTCTCAA
GATTGATAGGTGCACCTCCAGGATACGTGGGACATGATAAGGGAAGCTTTATAGCTGAGATACTTTTTAAACAAGGCAGT
TCACTTATCCTGTTTGATGAAATAGAAAAGGCACATGCTGATTTTCAAAACATTCTCCTTCAGATTCTTGATTATGGCTG
CATAACGGATAGCACTGGGAAAAAGAGTAGGTTCAAAAACTGCATAATGGCCTGCACGACAAACGTTGGAAGTACTTTTA
TTGAGAATAATTCTCTTGGTTTTACTCAAGATGGCAAGAAAACCGATAAGAAAGCTCAATTTGCAAGTTTTTTCAACAAA
GAACTACTTGATAGATTCGATCTTGCAATAGTCTTCAATACACTTGACAAAAATAGCGTGGAAAAAATAATAAATAAGTC
TGCAACTGAAATTCGTGCTCTCCTTGAAGAAAAAGGAATTAAGCTTAAGCTTACAGATGAAGTAGTAAAGTTACTAGTTG
CAAGGCACTCATCTGGTAAAATAAGTGCCAGAACTATAGAAAGGACCATCAGGAATGATATAATGCAGGCGGTTTCGGAT
AGGATGTTGCGTTCTAAAGCTAGCGAAATAGAGTTGACCATCAAGGACGGCTGTTTGGTGTAG

Upstream 100 bases:

>100_bases
CAATTATACAGGACTTAGGTCCGTCCGTTCGGTCGCACGGACTGGAACTGGGTATTTCACCCCTTAAAGAATAGTTCCTT
GTTTTGTCTCTTTTCACGCA

Downstream 100 bases:

>100_bases
GTATTCTTCTTGATTTTTGCAATTTTTTAGTCTTCACCAATATTTCCGTCCTACTTGCACTTTCTTATTTGCGTGGGGAA
CGTTTTGTGTTTATAGCATA

Product: ATP-dependent Clp protease, ATP-binding subunit ClpA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 740; Mature: 740

Protein sequence:

>740_residues
MISPSLENTLQNALVLARSFSHSYVTLEHLLLALTDDEEVSRIAFNSSMNFAKLRDSLIHFLSTAKFFARLSNECVDISK
IKPTLGLRRVIQRSKIHANIAEFGQITGLHVLIELFCEQDSYAFSFLTESNITLTELMSHVMKSRKGSPTGGTSTANTDA
LRSAELDNLKEYCTDMNELAIQGRIDKLIDRKKEIERTIEILLRRTKNNPIYVGEPGVGKTAIVEGLVHAIVSGNVPQKL
RNTRVYALKIGSLLAGTRYRGDFEERIRTVMNEISSSKNTILFIDEIHTIVGAGSTSNGSIDAGNLLKPFLTKGPLRCIG
ATTYREYSTHFEKDAALDRRFQRIEIEEPSISDTLNIIDGTKSYYEDFHRVHYTKASLNEIVNLAHRYIPNRAFPDKAID
LMDDLGASYNAELFDIKKRRDRKIDIKYVRETLAKRFNIPLAQLNYTVEEVLTSLERNLKAEIFDQDNIIEQVIYSLRPG
LLGLKGEKPLASYLFGGSTGVGKTELAKQIAKNLCMRFVRIDMSEYMEGHAVSRLIGAPPGYVGHDKGSFIAEILFKQGS
SLILFDEIEKAHADFQNILLQILDYGCITDSTGKKSRFKNCIMACTTNVGSTFIENNSLGFTQDGKKTDKKAQFASFFNK
ELLDRFDLAIVFNTLDKNSVEKIINKSATEIRALLEEKGIKLKLTDEVVKLLVARHSSGKISARTIERTIRNDIMQAVSD
RMLRSKASEIELTIKDGCLV

Sequences:

>Translated_740_residues
MISPSLENTLQNALVLARSFSHSYVTLEHLLLALTDDEEVSRIAFNSSMNFAKLRDSLIHFLSTAKFFARLSNECVDISK
IKPTLGLRRVIQRSKIHANIAEFGQITGLHVLIELFCEQDSYAFSFLTESNITLTELMSHVMKSRKGSPTGGTSTANTDA
LRSAELDNLKEYCTDMNELAIQGRIDKLIDRKKEIERTIEILLRRTKNNPIYVGEPGVGKTAIVEGLVHAIVSGNVPQKL
RNTRVYALKIGSLLAGTRYRGDFEERIRTVMNEISSSKNTILFIDEIHTIVGAGSTSNGSIDAGNLLKPFLTKGPLRCIG
ATTYREYSTHFEKDAALDRRFQRIEIEEPSISDTLNIIDGTKSYYEDFHRVHYTKASLNEIVNLAHRYIPNRAFPDKAID
LMDDLGASYNAELFDIKKRRDRKIDIKYVRETLAKRFNIPLAQLNYTVEEVLTSLERNLKAEIFDQDNIIEQVIYSLRPG
LLGLKGEKPLASYLFGGSTGVGKTELAKQIAKNLCMRFVRIDMSEYMEGHAVSRLIGAPPGYVGHDKGSFIAEILFKQGS
SLILFDEIEKAHADFQNILLQILDYGCITDSTGKKSRFKNCIMACTTNVGSTFIENNSLGFTQDGKKTDKKAQFASFFNK
ELLDRFDLAIVFNTLDKNSVEKIINKSATEIRALLEEKGIKLKLTDEVVKLLVARHSSGKISARTIERTIRNDIMQAVSD
RMLRSKASEIELTIKDGCLV
>Mature_740_residues
MISPSLENTLQNALVLARSFSHSYVTLEHLLLALTDDEEVSRIAFNSSMNFAKLRDSLIHFLSTAKFFARLSNECVDISK
IKPTLGLRRVIQRSKIHANIAEFGQITGLHVLIELFCEQDSYAFSFLTESNITLTELMSHVMKSRKGSPTGGTSTANTDA
LRSAELDNLKEYCTDMNELAIQGRIDKLIDRKKEIERTIEILLRRTKNNPIYVGEPGVGKTAIVEGLVHAIVSGNVPQKL
RNTRVYALKIGSLLAGTRYRGDFEERIRTVMNEISSSKNTILFIDEIHTIVGAGSTSNGSIDAGNLLKPFLTKGPLRCIG
ATTYREYSTHFEKDAALDRRFQRIEIEEPSISDTLNIIDGTKSYYEDFHRVHYTKASLNEIVNLAHRYIPNRAFPDKAID
LMDDLGASYNAELFDIKKRRDRKIDIKYVRETLAKRFNIPLAQLNYTVEEVLTSLERNLKAEIFDQDNIIEQVIYSLRPG
LLGLKGEKPLASYLFGGSTGVGKTELAKQIAKNLCMRFVRIDMSEYMEGHAVSRLIGAPPGYVGHDKGSFIAEILFKQGS
SLILFDEIEKAHADFQNILLQILDYGCITDSTGKKSRFKNCIMACTTNVGSTFIENNSLGFTQDGKKTDKKAQFASFFNK
ELLDRFDLAIVFNTLDKNSVEKIINKSATEIRALLEEKGIKLKLTDEVVKLLVARHSSGKISARTIERTIRNDIMQAVSD
RMLRSKASEIELTIKDGCLV

Specific function: ATP-Dependent Specificity Component Of The Clpp Protease. It Directs The Protease To Specific Substrates. The Primary Function Of The Clpa-Clpp Complex Appears To Be The Degradation Of Unfolded Or Abnormal Proteins. [C]

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=174, Percent_Identity=41.3793103448276, Blast_Score=132, Evalue=1e-30,
Organism=Escherichia coli, GI1787109, Length=730, Percent_Identity=40.2739726027397, Blast_Score=554, Evalue=1e-159,
Organism=Escherichia coli, GI1788943, Length=457, Percent_Identity=38.9496717724289, Blast_Score=284, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6323002, Length=392, Percent_Identity=35.7142857142857, Blast_Score=241, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6320464, Length=250, Percent_Identity=48.4, Blast_Score=232, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR013461
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 83121; Mature: 83121

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: PS00870 CLPAB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISPSLENTLQNALVLARSFSHSYVTLEHLLLALTDDEEVSRIAFNSSMNFAKLRDSLIH
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHH
FLSTAKFFARLSNECVDISKIKPTLGLRRVIQRSKIHANIAEFGQITGLHVLIELFCEQD
HHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SYAFSFLTESNITLTELMSHVMKSRKGSPTGGTSTANTDALRSAELDNLKEYCTDMNELA
CCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHH
IQGRIDKLIDRKKEIERTIEILLRRTKNNPIYVGEPGVGKTAIVEGLVHAIVSGNVPQKL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCHHHH
RNTRVYALKIGSLLAGTRYRGDFEERIRTVMNEISSSKNTILFIDEIHTIVGAGSTSNGS
HCCEEEEEEHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHHCCCCCCCCC
IDAGNLLKPFLTKGPLRCIGATTYREYSTHFEKDAALDRRFQRIEIEEPSISDTLNIIDG
CCCCHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHCC
TKSYYEDFHRVHYTKASLNEIVNLAHRYIPNRAFPDKAIDLMDDLGASYNAELFDIKKRR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHC
DRKIDIKYVRETLAKRFNIPLAQLNYTVEEVLTSLERNLKAEIFDQDNIIEQVIYSLRPG
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHCCC
LLGLKGEKPLASYLFGGSTGVGKTELAKQIAKNLCMRFVRIDMSEYMEGHAVSRLIGAPP
CCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCC
GYVGHDKGSFIAEILFKQGSSLILFDEIEKAHADFQNILLQILDYGCITDSTGKKSRFKN
CCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
CIMACTTNVGSTFIENNSLGFTQDGKKTDKKAQFASFFNKELLDRFDLAIVFNTLDKNSV
HHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHH
EKIINKSATEIRALLEEKGIKLKLTDEVVKLLVARHSSGKISARTIERTIRNDIMQAVSD
HHHHCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
RMLRSKASEIELTIKDGCLV
HHHHCCCHHEEEEECCCCCC
>Mature Secondary Structure
MISPSLENTLQNALVLARSFSHSYVTLEHLLLALTDDEEVSRIAFNSSMNFAKLRDSLIH
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHH
FLSTAKFFARLSNECVDISKIKPTLGLRRVIQRSKIHANIAEFGQITGLHVLIELFCEQD
HHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SYAFSFLTESNITLTELMSHVMKSRKGSPTGGTSTANTDALRSAELDNLKEYCTDMNELA
CCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHH
IQGRIDKLIDRKKEIERTIEILLRRTKNNPIYVGEPGVGKTAIVEGLVHAIVSGNVPQKL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCHHHH
RNTRVYALKIGSLLAGTRYRGDFEERIRTVMNEISSSKNTILFIDEIHTIVGAGSTSNGS
HCCEEEEEEHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHHCCCCCCCCC
IDAGNLLKPFLTKGPLRCIGATTYREYSTHFEKDAALDRRFQRIEIEEPSISDTLNIIDG
CCCCHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHCC
TKSYYEDFHRVHYTKASLNEIVNLAHRYIPNRAFPDKAIDLMDDLGASYNAELFDIKKRR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHC
DRKIDIKYVRETLAKRFNIPLAQLNYTVEEVLTSLERNLKAEIFDQDNIIEQVIYSLRPG
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHCCC
LLGLKGEKPLASYLFGGSTGVGKTELAKQIAKNLCMRFVRIDMSEYMEGHAVSRLIGAPP
CCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCC
GYVGHDKGSFIAEILFKQGSSLILFDEIEKAHADFQNILLQILDYGCITDSTGKKSRFKN
CCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
CIMACTTNVGSTFIENNSLGFTQDGKKTDKKAQFASFFNKELLDRFDLAIVFNTLDKNSV
HHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHH
EKIINKSATEIRALLEEKGIKLKLTDEVVKLLVARHSSGKISARTIERTIRNDIMQAVSD
HHHHCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
RMLRSKASEIELTIKDGCLV
HHHHCCCHHEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6209404 [H]