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The map label for this gene is sppA

Identifier: 88608447

GI number: 88608447

Start: 333406

End: 334332

Strand: Direct

Name: sppA

Synonym: NSE_0409

Alternate gene names: 88608447

Gene position: 333406-334332 (Clockwise)

Preceding gene: 88608293

Following gene: 88608815

Centisome position: 38.81

GC content: 44.01

Gene sequence:

>927_bases
ATGGATTTTTTGGGTGATGCGTTTGTCGAAAATTTTAGCTTAAGGAAGAGGTTAACTTTTTGGAAAGTGGTCACATTTGC
TCTGATTGCTCTCCTCTTGATTGTTTCGGGTTCTTCTTTTCGTGGTGGCTTTTTCGACAGTTTTTCCACCCCGTACATAG
GTAGGGTGGTCTTTTCTGGGGTTATAGATCAAGATCTTGCAAGAAATAGCCAGTTTGCTTCTTTTGCGGACAATCCTAAA
ATAAAAGCAATAATCCTTCACGTAGACAGCGGAGGTGGGGGAGCGGCCGCTTCTGAAGCGCTTTACAACGTTGTTAGGAG
CGTGTCTCAAGTTAAGCCGGTTGTGATTGTAGCCAACGGAATAATGGCTTCTGGTGCGTACATGGTAGCAATGGCTGCTG
AACATATAGTCGCATACAATACTAGTATTGTCGGTTCGATTGGTATGATATTACAAGCCCCGAATTTTTATGAGATCGGA
AAGAAAGTTGGGGTAAAAATGGACGTTGTTCGTTCTGGTAGATTGAAAGCCTTCCCATCTATTTTGGAAGAATTTACCAA
AGAGGCGCGTTTGGCTATGGAGCACTCCATCAGCGTCGCTAACGAGCACTTTTTATCTATGGTACAGGAGCGCAGAAAGA
TTACAGATGCCCGTGTGATGTCCGAGATTGCGACAGGTAAGATCTTTACCGGCAGGGAAGCGCTTGAGTTTTCCCTGGTT
GACGAGATAGGTGATGAGGGAAATGCGGTAAGGTGGCTGAAGGAAAGGGGAATTACGGGAAAAATACGTGATCTTGGTTT
TGTTTCAGGTAAGGGTTTTCGTGTAGGTAAGATTCCCCCATCTTCTTATCTTTCCATTTTAAATGAGTTTTTCCATTACT
TGTGTTCTCCAGGGCTTCTTGCCGTTTTTCCGGGTATCTCTACTTAG

Upstream 100 bases:

>100_bases
GTAGTAAAGATCATTATTCAGTTAAGCTGGAGATTATCACAGACGAGAAGGCTGGTGGGGCTCCTGCTGGAGAAGAGAAG
TGAGAGGTGGTTATTGGCAC

Downstream 100 bases:

>100_bases
CCTGGGTTTTTCTTTCTTCCTACTTCCTGTTTTTCTGTTGAAATATTGATTTTGTCACTCCTGTATTTCATTTGAGTCAT
GCCTAGTGGTATTTTGTGGT

Product: signal peptide peptidase SppA, 36K type

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MDFLGDAFVENFSLRKRLTFWKVVTFALIALLLIVSGSSFRGGFFDSFSTPYIGRVVFSGVIDQDLARNSQFASFADNPK
IKAIILHVDSGGGGAAASEALYNVVRSVSQVKPVVIVANGIMASGAYMVAMAAEHIVAYNTSIVGSIGMILQAPNFYEIG
KKVGVKMDVVRSGRLKAFPSILEEFTKEARLAMEHSISVANEHFLSMVQERRKITDARVMSEIATGKIFTGREALEFSLV
DEIGDEGNAVRWLKERGITGKIRDLGFVSGKGFRVGKIPPSSYLSILNEFFHYLCSPGLLAVFPGIST

Sequences:

>Translated_308_residues
MDFLGDAFVENFSLRKRLTFWKVVTFALIALLLIVSGSSFRGGFFDSFSTPYIGRVVFSGVIDQDLARNSQFASFADNPK
IKAIILHVDSGGGGAAASEALYNVVRSVSQVKPVVIVANGIMASGAYMVAMAAEHIVAYNTSIVGSIGMILQAPNFYEIG
KKVGVKMDVVRSGRLKAFPSILEEFTKEARLAMEHSISVANEHFLSMVQERRKITDARVMSEIATGKIFTGREALEFSLV
DEIGDEGNAVRWLKERGITGKIRDLGFVSGKGFRVGKIPPSSYLSILNEFFHYLCSPGLLAVFPGIST
>Mature_308_residues
MDFLGDAFVENFSLRKRLTFWKVVTFALIALLLIVSGSSFRGGFFDSFSTPYIGRVVFSGVIDQDLARNSQFASFADNPK
IKAIILHVDSGGGGAAASEALYNVVRSVSQVKPVVIVANGIMASGAYMVAMAAEHIVAYNTSIVGSIGMILQAPNFYEIG
KKVGVKMDVVRSGRLKAFPSILEEFTKEARLAMEHSISVANEHFLSMVQERRKITDARVMSEIATGKIFTGREALEFSLV
DEIGDEGNAVRWLKERGITGKIRDLGFVSGKGFRVGKIPPSSYLSILNEFFHYLCSPGLLAVFPGIST

Specific function: Digestion Of The Cleaved Signal Peptides. This Activity Is Necessary To Maintain Proper Secretion Of Mature Proteins Across The Membrane. [C]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=174, Percent_Identity=35.0574712643678, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787527, Length=204, Percent_Identity=29.4117647058824, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 33525; Mature: 33525

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFLGDAFVENFSLRKRLTFWKVVTFALIALLLIVSGSSFRGGFFDSFSTPYIGRVVFSG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
VIDQDLARNSQFASFADNPKIKAIILHVDSGGGGAAASEALYNVVRSVSQVKPVVIVANG
HHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEECC
IMASGAYMVAMAAEHIVAYNTSIVGSIGMILQAPNFYEIGKKVGVKMDVVRSGRLKAFPS
HHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHCCEEEEHHCCCHHHHHH
ILEEFTKEARLAMEHSISVANEHFLSMVQERRKITDARVMSEIATGKIFTGREALEFSLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHH
DEIGDEGNAVRWLKERGITGKIRDLGFVSGKGFRVGKIPPSSYLSILNEFFHYLCSPGLL
HHCCCCCCHHHHHHHCCCCCCHHHCEEECCCCEEECCCCHHHHHHHHHHHHHHHCCCCCE
AVFPGIST
ECCCCCCC
>Mature Secondary Structure
MDFLGDAFVENFSLRKRLTFWKVVTFALIALLLIVSGSSFRGGFFDSFSTPYIGRVVFSG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
VIDQDLARNSQFASFADNPKIKAIILHVDSGGGGAAASEALYNVVRSVSQVKPVVIVANG
HHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEECC
IMASGAYMVAMAAEHIVAYNTSIVGSIGMILQAPNFYEIGKKVGVKMDVVRSGRLKAFPS
HHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHCCEEEEHHCCCHHHHHH
ILEEFTKEARLAMEHSISVANEHFLSMVQERRKITDARVMSEIATGKIFTGREALEFSLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHH
DEIGDEGNAVRWLKERGITGKIRDLGFVSGKGFRVGKIPPSSYLSILNEFFHYLCSPGLL
HHCCCCCCHHHHHHHCCCCCCHHHCEEECCCCEEECCCCHHHHHHHHHHHHHHHCCCCCE
AVFPGIST
ECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]