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The map label for this gene is gph [C]

Identifier: 88608430

GI number: 88608430

Start: 824862

End: 825506

Strand: Direct

Name: gph [C]

Synonym: NSE_0934

Alternate gene names: 88608430

Gene position: 824862-825506 (Clockwise)

Preceding gene: 88608651

Following gene: 88608041

Centisome position: 96.03

GC content: 40.78

Gene sequence:

>645_bases
ATGAATGAAAAATTGAACACCAAGGCAAAAGTCACTCCTGAAGCAATATTATTTGATTGGGATAACACTATTGTTGATAC
ACGAGTCATGATCCTAAATGCAGTTAACAAAACGCTGAGTCACTTTGGCAAACCAGCGCTTGATAGAGACATGAATGCAC
CGGAAAGAATGGATTTCTTTAAACAGACTTTTGGAGAGGAATGGGTTTTCGCTTATTCGCTATTCACAGATCACCTTAGA
TCCGAGAACCCGGGACCGCTACAGATATTTCCAGGATTCACATCATTTTTGGATTTGATAGCTGAAAGGCGCATATTTAC
GGGTATAGTCAGCAATAAATATAACGAACTACTCAAAAAAGAGGTCGATTCTCTCGGACTAACGGATCGATTTGATGTCA
TAGTCGGCTCAGGTGATACAGACCAGGATAAACCTTCAGCAAAACCACTACTGTACGCTCTATCGATAGGTAACATTACT
CCATCGAAAGAAACTGTTCTTTTTATCGGTGACAGCGCAACGGACATGGAGTGCGCAAGGAATGCAACTGTACGTGGTAT
TGCATATCGAGCGACTCCAATAGAAGGCTTTGAGAATATACCAATCATACGGCATTATGACCAGGCAAAAATATTAATAC
CCTAA

Upstream 100 bases:

>100_bases
GCTGTGACAAATAACATAGCCAAAACTTTAATAAGTTGTGATGAAATATCACTGAAGGATTGCATTAGAACACATAGTAC
TGACAATCATACCCAACATA

Downstream 100 bases:

>100_bases
GGATATACCAGGTCCTATTGACAAAGAGCACTTCAATGTAATCAAATGGCACTAGTCTTACCTTATCTGCATGTATAGCA
AATCGAGTTATCTCATGATT

Product: HAD family hydrolase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MNEKLNTKAKVTPEAILFDWDNTIVDTRVMILNAVNKTLSHFGKPALDRDMNAPERMDFFKQTFGEEWVFAYSLFTDHLR
SENPGPLQIFPGFTSFLDLIAERRIFTGIVSNKYNELLKKEVDSLGLTDRFDVIVGSGDTDQDKPSAKPLLYALSIGNIT
PSKETVLFIGDSATDMECARNATVRGIAYRATPIEGFENIPIIRHYDQAKILIP

Sequences:

>Translated_214_residues
MNEKLNTKAKVTPEAILFDWDNTIVDTRVMILNAVNKTLSHFGKPALDRDMNAPERMDFFKQTFGEEWVFAYSLFTDHLR
SENPGPLQIFPGFTSFLDLIAERRIFTGIVSNKYNELLKKEVDSLGLTDRFDVIVGSGDTDQDKPSAKPLLYALSIGNIT
PSKETVLFIGDSATDMECARNATVRGIAYRATPIEGFENIPIIRHYDQAKILIP
>Mature_214_residues
MNEKLNTKAKVTPEAILFDWDNTIVDTRVMILNAVNKTLSHFGKPALDRDMNAPERMDFFKQTFGEEWVFAYSLFTDHLR
SENPGPLQIFPGFTSFLDLIAERRIFTGIVSNKYNELLKKEVDSLGLTDRFDVIVGSGDTDQDKPSAKPLLYALSIGNIT
PSKETVLFIGDSATDMECARNATVRGIAYRATPIEGFENIPIIRHYDQAKILIP

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 24108; Mature: 24108

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEKLNTKAKVTPEAILFDWDNTIVDTRVMILNAVNKTLSHFGKPALDRDMNAPERMDFF
CCCCCCCCEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
KQTFGEEWVFAYSLFTDHLRSENPGPLQIFPGFTSFLDLIAERRIFTGIVSNKYNELLKK
HHHCCCCHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVDSLGLTDRFDVIVGSGDTDQDKPSAKPLLYALSIGNITPSKETVLFIGDSATDMECAR
HHHHCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCHHHHC
NATVRGIAYRATPIEGFENIPIIRHYDQAKILIP
CCCEEEEEEECCCCCCCCCCCEEEECCCEEEECC
>Mature Secondary Structure
MNEKLNTKAKVTPEAILFDWDNTIVDTRVMILNAVNKTLSHFGKPALDRDMNAPERMDFF
CCCCCCCCEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
KQTFGEEWVFAYSLFTDHLRSENPGPLQIFPGFTSFLDLIAERRIFTGIVSNKYNELLKK
HHHCCCCHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVDSLGLTDRFDVIVGSGDTDQDKPSAKPLLYALSIGNITPSKETVLFIGDSATDMECAR
HHHHCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCHHHHC
NATVRGIAYRATPIEGFENIPIIRHYDQAKILIP
CCCEEEEEEECCCCCCCCCCCEEEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782 [H]