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The map label for this gene is eno

Identifier: 88608407

GI number: 88608407

Start: 653439

End: 654680

Strand: Reverse

Name: eno

Synonym: NSE_0733

Alternate gene names: 88608407

Gene position: 654680-653439 (Counterclockwise)

Preceding gene: 88608691

Following gene: 88608000

Centisome position: 76.21

GC content: 43.8

Gene sequence:

>1242_bases
ATGCAGCAAATACAAAAAGTAAAAGCCCGTGAGATTTTCAATAGTAGGGGGTGGCCTACAATCGAGGTTGAAGTTACAAC
TAGTTGCGGTAGAGTCGGGTGCGCAATAGCACCTTCTGGTGCATCAAAAGGAGTTCTAGAGGCCTGTGAATTGCTCGATG
GCGATAAAGCGCGTCTGTCTGGGAGAGGTGTCTTGCGTGCTGTAGAAAATGTCCACACGATTATCGCTCCGGCCCTTATG
GGGAAGAGTGTCGCTGCTCAAGCCGAAATAGATTCTCTTTTGATTCAATTAGACGGTACGACAAATAAGTCTGTGCTTGG
AGCTAATGCAATCGTGGCTGTTTCTCTTGCAGTTGCGAAGGCAAATGCAAGTGTTAGGGGCGTTCCTTTGTGTATTGTTT
TTCATAACTTACTCACCAGCGAGGGTGACGATTTTGTATATGTTCCTCCTGTTCCGATGTTAAATGTCATAAATGGTGGC
GTGCATGCCGATAATCGGTTGGCAATCCAAGAGTTCATGATTTGCCCGGTTGGACGTCGTTCCTTTAGAGAAAGTATGGA
GAAGGCAGCAGACGTCTTTCACCGTTTGAAGGGGCTTCTAAAACAATATGGGAAGAGTACGAATGTTGGTGACGAGGGTG
GTTTCGCGCCTGATCTTTCTTCTACTGAAGAGACGCTTGGACTTCTCTCTGAGGCCATTGGTGATAGTAAAGAGAGCGTT
AAAATAGCTCTTGATGCAGCTTCTTCGACTTTTTATAAAGATGGAAAATATAGTATCGATGGAAAATTGTTGAATGTTAA
CGAAATGGTCGATTTCTATGCTGCGATCATAGAAAAGTACGATATTGTTTCTATCGAGGACCCGCTGTACGAGAGTGATT
GGGAGTCCTGGCAGGTAATGACGAGAAAGCTTTCTGATAAAATTCACATAGTCGGGGATGATATTTTTGTTACCAATCCT
AAGATAATAAAAAAGGGAATAAAAACTGGTGTTGCCAATGCGGTGCTTGTGAAAATTAACCAAGTTGGTACCGTTACAGA
GACGATTGAGAGCATAAAACTGGCTAGAAAAGCTGGATATAAAGTGGTCATTTCACACCGTTCAGGAGAGACCGAGGATC
TATCAATAGCACATCTAGCTGTTGCTTGTGGAGGTGCTTTTCTCAAGGCTGGTTCTTTAAGTCGCTCAGAGAGAGTTGCT
AAGTACAATGAGGTACTTCGTTTGGAGGAGGTATTTGTGTGA

Upstream 100 bases:

>100_bases
GATACAGCTATAATGCTTCATATCCTTCTCACGAATTGCTAGAATGAATGTTTAGCAGAAGTACTGAGAACAAACAAGCG
TTTCTGTATCCCGTTAACTT

Downstream 100 bases:

>100_bases
TGTTTAGAGTTATCTGCGTTGTTTTTCTTCTTTCTTTTTTGTCTTTGCCATCTTACTCCATTGAAGTTAAAACTGCTGCT
ATGGTTGGGGATGTGATAAT

Product: enolase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MQQIQKVKAREIFNSRGWPTIEVEVTTSCGRVGCAIAPSGASKGVLEACELLDGDKARLSGRGVLRAVENVHTIIAPALM
GKSVAAQAEIDSLLIQLDGTTNKSVLGANAIVAVSLAVAKANASVRGVPLCIVFHNLLTSEGDDFVYVPPVPMLNVINGG
VHADNRLAIQEFMICPVGRRSFRESMEKAADVFHRLKGLLKQYGKSTNVGDEGGFAPDLSSTEETLGLLSEAIGDSKESV
KIALDAASSTFYKDGKYSIDGKLLNVNEMVDFYAAIIEKYDIVSIEDPLYESDWESWQVMTRKLSDKIHIVGDDIFVTNP
KIIKKGIKTGVANAVLVKINQVGTVTETIESIKLARKAGYKVVISHRSGETEDLSIAHLAVACGGAFLKAGSLSRSERVA
KYNEVLRLEEVFV

Sequences:

>Translated_413_residues
MQQIQKVKAREIFNSRGWPTIEVEVTTSCGRVGCAIAPSGASKGVLEACELLDGDKARLSGRGVLRAVENVHTIIAPALM
GKSVAAQAEIDSLLIQLDGTTNKSVLGANAIVAVSLAVAKANASVRGVPLCIVFHNLLTSEGDDFVYVPPVPMLNVINGG
VHADNRLAIQEFMICPVGRRSFRESMEKAADVFHRLKGLLKQYGKSTNVGDEGGFAPDLSSTEETLGLLSEAIGDSKESV
KIALDAASSTFYKDGKYSIDGKLLNVNEMVDFYAAIIEKYDIVSIEDPLYESDWESWQVMTRKLSDKIHIVGDDIFVTNP
KIIKKGIKTGVANAVLVKINQVGTVTETIESIKLARKAGYKVVISHRSGETEDLSIAHLAVACGGAFLKAGSLSRSERVA
KYNEVLRLEEVFV
>Mature_413_residues
MQQIQKVKAREIFNSRGWPTIEVEVTTSCGRVGCAIAPSGASKGVLEACELLDGDKARLSGRGVLRAVENVHTIIAPALM
GKSVAAQAEIDSLLIQLDGTTNKSVLGANAIVAVSLAVAKANASVRGVPLCIVFHNLLTSEGDDFVYVPPVPMLNVINGG
VHADNRLAIQEFMICPVGRRSFRESMEKAADVFHRLKGLLKQYGKSTNVGDEGGFAPDLSSTEETLGLLSEAIGDSKESV
KIALDAASSTFYKDGKYSIDGKLLNVNEMVDFYAAIIEKYDIVSIEDPLYESDWESWQVMTRKLSDKIHIVGDDIFVTNP
KIIKKGIKTGVANAVLVKINQVGTVTETIESIKLARKAGYKVVISHRSGETEDLSIAHLAVACGGAFLKAGSLSRSERVA
KYNEVLRLEEVFV

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI4503571, Length=420, Percent_Identity=49.5238095238095, Blast_Score=381, Evalue=1e-106,
Organism=Homo sapiens, GI301897477, Length=422, Percent_Identity=48.1042654028436, Blast_Score=379, Evalue=1e-105,
Organism=Homo sapiens, GI301897469, Length=422, Percent_Identity=48.1042654028436, Blast_Score=379, Evalue=1e-105,
Organism=Homo sapiens, GI5803011, Length=420, Percent_Identity=48.0952380952381, Blast_Score=377, Evalue=1e-105,
Organism=Homo sapiens, GI301897479, Length=420, Percent_Identity=43.0952380952381, Blast_Score=317, Evalue=1e-86,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=26.4880952380952, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=26.4880952380952, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=26.4880952380952, Blast_Score=103, Evalue=4e-22,
Organism=Escherichia coli, GI1789141, Length=419, Percent_Identity=51.3126491646778, Blast_Score=407, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17536383, Length=421, Percent_Identity=48.4560570071259, Blast_Score=363, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI71995829, Length=421, Percent_Identity=48.4560570071259, Blast_Score=363, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32563855, Length=179, Percent_Identity=44.6927374301676, Blast_Score=160, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6324974, Length=421, Percent_Identity=48.4560570071259, Blast_Score=368, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6324969, Length=421, Percent_Identity=48.4560570071259, Blast_Score=368, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6323985, Length=421, Percent_Identity=48.2185273159145, Blast_Score=366, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6321693, Length=421, Percent_Identity=47.9809976247031, Blast_Score=357, Evalue=2e-99,
Organism=Saccharomyces cerevisiae, GI6321968, Length=421, Percent_Identity=47.2684085510689, Blast_Score=325, Evalue=7e-90,
Organism=Drosophila melanogaster, GI24580918, Length=421, Percent_Identity=47.0308788598575, Blast_Score=345, Evalue=3e-95,
Organism=Drosophila melanogaster, GI24580916, Length=421, Percent_Identity=47.0308788598575, Blast_Score=345, Evalue=3e-95,
Organism=Drosophila melanogaster, GI24580920, Length=421, Percent_Identity=47.0308788598575, Blast_Score=345, Evalue=3e-95,
Organism=Drosophila melanogaster, GI24580914, Length=421, Percent_Identity=47.0308788598575, Blast_Score=345, Evalue=3e-95,
Organism=Drosophila melanogaster, GI281360527, Length=421, Percent_Identity=47.0308788598575, Blast_Score=345, Evalue=3e-95,
Organism=Drosophila melanogaster, GI17137654, Length=421, Percent_Identity=47.0308788598575, Blast_Score=345, Evalue=3e-95,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_NEOSM (Q2GD37)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506609.1
- HSSP:   P0A6P9
- ProteinModelPortal:   Q2GD37
- SMR:   Q2GD37
- STRING:   Q2GD37
- GeneID:   3932036
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0733
- TIGR:   NSE_0733
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   CLSK2527900
- BioCyc:   NSEN222891:NSE_0733-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 44482; Mature: 44482

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 212-212 ACT_SITE 338-338 BINDING 162-162 BINDING 171-171 BINDING 286-286 BINDING 313-313 BINDING 338-338 BINDING 389-389

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQIQKVKAREIFNSRGWPTIEVEVTTSCGRVGCAIAPSGASKGVLEACELLDGDKARLS
CCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEECCCCCCCHHHHHHHHCCCCHHHHC
GRGVLRAVENVHTIIAPALMGKSVAAQAEIDSLLIQLDGTTNKSVLGANAIVAVSLAVAK
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCEEEEEECCCCCCCEECCCHHHHHEEHHHH
ANASVRGVPLCIVFHNLLTSEGDDFVYVPPVPMLNVINGGVHADNRLAIQEFMICPVGRR
CCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHCCCCCCCCHHHHHHHHHCCCCHH
SFRESMEKAADVFHRLKGLLKQYGKSTNVGDEGGFAPDLSSTEETLGLLSEAIGDSKESV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCE
KIALDAASSTFYKDGKYSIDGKLLNVNEMVDFYAAIIEKYDIVSIEDPLYESDWESWQVM
EEEEEECCCCEECCCCEEECCEEEEHHHHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHH
TRKLSDKIHIVGDDIFVTNPKIIKKGIKTGVANAVLVKINQVGTVTETIESIKLARKAGY
HHHHCCCEEEEECCEEECCHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCC
KVVISHRSGETEDLSIAHLAVACGGAFLKAGSLSRSERVAKYNEVLRLEEVFV
EEEEEECCCCCCCHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQQIQKVKAREIFNSRGWPTIEVEVTTSCGRVGCAIAPSGASKGVLEACELLDGDKARLS
CCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEECCCCCCCHHHHHHHHCCCCHHHHC
GRGVLRAVENVHTIIAPALMGKSVAAQAEIDSLLIQLDGTTNKSVLGANAIVAVSLAVAK
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCEEEEEECCCCCCCEECCCHHHHHEEHHHH
ANASVRGVPLCIVFHNLLTSEGDDFVYVPPVPMLNVINGGVHADNRLAIQEFMICPVGRR
CCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHCCCCCCCCHHHHHHHHHCCCCHH
SFRESMEKAADVFHRLKGLLKQYGKSTNVGDEGGFAPDLSSTEETLGLLSEAIGDSKESV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCE
KIALDAASSTFYKDGKYSIDGKLLNVNEMVDFYAAIIEKYDIVSIEDPLYESDWESWQVM
EEEEEECCCCEECCCCEEECCEEEEHHHHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHH
TRKLSDKIHIVGDDIFVTNPKIIKKGIKTGVANAVLVKINQVGTVTETIESIKLARKAGY
HHHHCCCEEEEECCEEECCHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCC
KVVISHRSGETEDLSIAHLAVACGGAFLKAGSLSRSERVAKYNEVLRLEEVFV
EEEEEECCCCCCCHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA