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The map label for this gene is hemK [H]

Identifier: 88608405

GI number: 88608405

Start: 105557

End: 106426

Strand: Direct

Name: hemK [H]

Synonym: NSE_0128

Alternate gene names: 88608405

Gene position: 105557-106426 (Clockwise)

Preceding gene: 88608113

Following gene: 88608469

Centisome position: 12.29

GC content: 42.53

Gene sequence:

>870_bases
ATGCCTCAGATGAAAGAGAAAGATTATGATTCAGATATGAGCATCCGGGCCAGGCTAATAGCAGGGAGTAGGTATCTTGC
AAATTGGTCCTCTACTCCGCACCTTGATGCAGAGCTCTTACTTGCTCATGTACTATCTTTGAATCGTGAGCAACTGATAC
TGTATTGCAACGAGGGGATGACAGACTCCCAAGCAATGGATTTCGAAATGTTGTTGAAACTGAGAAAAAGTCATTCTGTG
GCGGCCATTGTTGGTGAAAAAGAATTCTGGAAGCACAGCTTCGCTGTTAATAAGGACGTTCTGATTCCGCGCCCAGATAC
AGAAACTATGCTAGTAGCTCTTCTATCACGCTATAAAAAGCTTACTCAGCCTCTGAAAATAGTTGAGCTAGGTACCGGGA
GTGGGTGTGTTATTATTTCCATACTGAAGGAATTCCGAAATGCGTTGGGGTTTGGTTTTGAAAAGTCAAGGGCTGCATTT
TATCTCACAATGCATAACATGCTTAAATATGGTTTGCGTGCTCGTTTGAAGCTATATAGGCTCGGCTTTGAAAGCGCAAT
GAGGGTCCTTTCATGCAAAGTGGACGTAATTGTTAGCAATCCCCCCTACGTAAGACGCGGTGAAATTCCCTATCTCCAGC
TAGAAGTGCAAAACGAGCCCCGCATTGCGTTGGATGGAGGGTTTAATGGTATATTGCCCTATTTCTCTATCTTGAAACTT
GCAAGCAAGATATTGCGTCCAGGTGGAGAAATTTTTCTAGAAATTGGTAGCAGCTGGAGAGATTCGGTAGTGTCAGCTAA
CTATCCTTTTAAAATTGTTGAAAGGTACCGAGATTTATCTGGAATTGAGCGTATTCTTGTTTTAAAATAA

Upstream 100 bases:

>100_bases
AGGAAAGTAAAGAAAATTCGGATGAATAGGAAAATGCGGTTAATGTTGTGATTACAGTAGGAGTGCTAAGTGCTATATCC
AGGTATGAGACTAATCTACG

Downstream 100 bases:

>100_bases
GTCTACCGCGAGACATTTAGGTGTGATCCGCCGATCTCATTCTTCATTTCACTCTGCTATCAAGTAACTAAAAAATATAT
TTTAAAATGTACTCTTTAAA

Product: HemK family modification methylase

Products: NA

Alternate protein names: HemK protein homolog; M.RprHemKP; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MPQMKEKDYDSDMSIRARLIAGSRYLANWSSTPHLDAELLLAHVLSLNREQLILYCNEGMTDSQAMDFEMLLKLRKSHSV
AAIVGEKEFWKHSFAVNKDVLIPRPDTETMLVALLSRYKKLTQPLKIVELGTGSGCVIISILKEFRNALGFGFEKSRAAF
YLTMHNMLKYGLRARLKLYRLGFESAMRVLSCKVDVIVSNPPYVRRGEIPYLQLEVQNEPRIALDGGFNGILPYFSILKL
ASKILRPGGEIFLEIGSSWRDSVVSANYPFKIVERYRDLSGIERILVLK

Sequences:

>Translated_289_residues
MPQMKEKDYDSDMSIRARLIAGSRYLANWSSTPHLDAELLLAHVLSLNREQLILYCNEGMTDSQAMDFEMLLKLRKSHSV
AAIVGEKEFWKHSFAVNKDVLIPRPDTETMLVALLSRYKKLTQPLKIVELGTGSGCVIISILKEFRNALGFGFEKSRAAF
YLTMHNMLKYGLRARLKLYRLGFESAMRVLSCKVDVIVSNPPYVRRGEIPYLQLEVQNEPRIALDGGFNGILPYFSILKL
ASKILRPGGEIFLEIGSSWRDSVVSANYPFKIVERYRDLSGIERILVLK
>Mature_288_residues
PQMKEKDYDSDMSIRARLIAGSRYLANWSSTPHLDAELLLAHVLSLNREQLILYCNEGMTDSQAMDFEMLLKLRKSHSVA
AIVGEKEFWKHSFAVNKDVLIPRPDTETMLVALLSRYKKLTQPLKIVELGTGSGCVIISILKEFRNALGFGFEKSRAAFY
LTMHNMLKYGLRARLKLYRLGFESAMRVLSCKVDVIVSNPPYVRRGEIPYLQLEVQNEPRIALDGGFNGILPYFSILKLA
SKILRPGGEIFLEIGSSWRDSVVSANYPFKIVERYRDLSGIERILVLK

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=274, Percent_Identity=29.9270072992701, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI1787463, Length=261, Percent_Identity=29.1187739463602, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI87082085, Length=184, Percent_Identity=29.3478260869565, Blast_Score=71, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24582226, Length=205, Percent_Identity=31.7073170731707, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4; PF05175 MTS [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 32911; Mature: 32780

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQMKEKDYDSDMSIRARLIAGSRYLANWSSTPHLDAELLLAHVLSLNREQLILYCNEGM
CCCCCCCCCCCCCCEEEEEEECCEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCC
TDSQAMDFEMLLKLRKSHSVAAIVGEKEFWKHSFAVNKDVLIPRPDTETMLVALLSRYKK
CCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHH
LTQPLKIVELGTGSGCVIISILKEFRNALGFGFEKSRAAFYLTMHNMLKYGLRARLKLYR
HCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHH
LGFESAMRVLSCKVDVIVSNPPYVRRGEIPYLQLEVQNEPRIALDGGFNGILPYFSILKL
HCHHHHHHHHHEEEEEEECCCCCCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHH
ASKILRPGGEIFLEIGSSWRDSVVSANYPFKIVERYRDLSGIERILVLK
HHHHHCCCCEEEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHEEEEEC
>Mature Secondary Structure 
PQMKEKDYDSDMSIRARLIAGSRYLANWSSTPHLDAELLLAHVLSLNREQLILYCNEGM
CCCCCCCCCCCCCEEEEEEECCEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCC
TDSQAMDFEMLLKLRKSHSVAAIVGEKEFWKHSFAVNKDVLIPRPDTETMLVALLSRYKK
CCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHH
LTQPLKIVELGTGSGCVIISILKEFRNALGFGFEKSRAAFYLTMHNMLKYGLRARLKLYR
HCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHH
LGFESAMRVLSCKVDVIVSNPPYVRRGEIPYLQLEVQNEPRIALDGGFNGILPYFSILKL
HCHHHHHHHHHEEEEEEECCCCCCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHH
ASKILRPGGEIFLEIGSSWRDSVVSANYPFKIVERYRDLSGIERILVLK
HHHHHCCCCEEEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]