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The map label for this gene is opuE [H]

Identifier: 88608369

GI number: 88608369

Start: 645945

End: 647351

Strand: Direct

Name: opuE [H]

Synonym: NSE_0724

Alternate gene names: 88608369

Gene position: 645945-647351 (Clockwise)

Preceding gene: 88608278

Following gene: 88607990

Centisome position: 75.2

GC content: 40.16

Gene sequence:

>1407_bases
ATGTTTTACACTCACCTACTTGTGGTAGGACTATACCTTCTCTGTATTTTTCTCTTTGGGTTCGTTCGTAAGAGGGTTAG
TGCATCATTTAAAGAATATATTCTTGCAAATAGGTCGGTGCACGAAATAATCACTGGAATAAGTCATGTTTCTTCCGAAA
TCAGCGTAGGAACCTTTACTGTAGTGGCAAGCATGATTTACACAACTGGGTTCTCGCGGCTTTGGGTCGATCTAGGATTC
TTTGTGTTTAGCATACTCTCCTGGCGACTCTTAGCCGGACGTTTGAGAGTACAGAGTGAAGAGAGCAAGGACGCGCTTAC
GATTCCGCAATATCTTGCAAACAAATTTGATAGCAAAACGGTTGGAATTTTTGCATCACTAATCATTACTCTCTTCACCA
CGACGTACATTGGTGCAAATATGCTTGGTCTTACAAAAGTTCTTTCCAAAGTGGTAAGCCTGGAGTCCAACTTAGTAGCA
GTAGCGGTGCTGATTGTTACATTGTTGTATACTACTGTAGGCGGCTTTAGATCTGCTTCCCATGTGAACGTTCTTCAGGG
GGCAATAATGCTTTTAGCGACTTGCATACTACCGCTCTCACTACTAATAAAGTTCGGCTTAGAACCACTAACCACTTTCT
TAAAACAGAGTGGGTCAAGTGCCGTTGGTCATCTCGACATTACACAAGCAGTATTTTACTTCAGCTTCGCCTCGGTAATG
TTTGGAACACCACACACAATTGGAAAATTCTTAGCTATAAAATCTCCTGACAAATTGAACACTGCTCGGAATACAAGCAT
TATCATAAACATCATAATCTACCTTGGCATAGCAATATCTGCAGTGTACGGAAGAAAATTACTTCCATCTATGAAAGATA
CAGAAATGCTACTTTTGAACCTAAGCGAAATGTTTACCAGTCCACTTTACGGTAGCATGATAATAATCGCGATCCTGGCC
GCCGTAATGAGTACGATAGATGCTCAACTGATATCAAGTGCAAGCTGCATAGCTAACGATATCTATAGACACATTACATC
GAACAGAAATGATATACATCTTATTTTCGCCACAAAACTTATGATAATATTTGTAGGTGCATTAGCCCTTTCAATCTCAT
TAATTTTCAACCAATCTGTTGGAAAACTAGCAATCTTTTCTTTGACAGGATTAGGGGCTTCAATAGGACCAGTGATATTA
TTCAGTCTGTACACAAAAACCAGAAATAAAATACCAGCTCTGCTTGGCTTGACTTCTGGAACAACGGCAACAATACTTTT
GACAATCTTTAACACCTCTGACATTCACTACAACATGTTGCCTGCGTTGTGTATTACGGCTGTAGTAATGCTAGCCTCGT
CCTGTATAACTGGACGCACGGTGAAATGTGTTGAGTCTAACAATTAA

Upstream 100 bases:

>100_bases
TTTATGAGAAGTATTTTCCTTTTCTTTGTCACATGTAATTAATGTATTTAGCTTTGTTTAAATTGCTGCCTGAAGCTGCT
CGCTGAAATTGCATCTGTAA

Downstream 100 bases:

>100_bases
AGTGATTGTATCTAGCCCGAATTGGTTGCCTATGAAAAATTTGATCCCGTTTTTTTTAGTCGTTATTGCTTTTTTCGCTT
TTTCTGATTGTTACGCTGCC

Product: putative sodium:proline symporter

Products: NA

Alternate protein names: Sodium/proline symporter [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MFYTHLLVVGLYLLCIFLFGFVRKRVSASFKEYILANRSVHEIITGISHVSSEISVGTFTVVASMIYTTGFSRLWVDLGF
FVFSILSWRLLAGRLRVQSEESKDALTIPQYLANKFDSKTVGIFASLIITLFTTTYIGANMLGLTKVLSKVVSLESNLVA
VAVLIVTLLYTTVGGFRSASHVNVLQGAIMLLATCILPLSLLIKFGLEPLTTFLKQSGSSAVGHLDITQAVFYFSFASVM
FGTPHTIGKFLAIKSPDKLNTARNTSIIINIIIYLGIAISAVYGRKLLPSMKDTEMLLLNLSEMFTSPLYGSMIIIAILA
AVMSTIDAQLISSASCIANDIYRHITSNRNDIHLIFATKLMIIFVGALALSISLIFNQSVGKLAIFSLTGLGASIGPVIL
FSLYTKTRNKIPALLGLTSGTTATILLTIFNTSDIHYNMLPALCITAVVMLASSCITGRTVKCVESNN

Sequences:

>Translated_468_residues
MFYTHLLVVGLYLLCIFLFGFVRKRVSASFKEYILANRSVHEIITGISHVSSEISVGTFTVVASMIYTTGFSRLWVDLGF
FVFSILSWRLLAGRLRVQSEESKDALTIPQYLANKFDSKTVGIFASLIITLFTTTYIGANMLGLTKVLSKVVSLESNLVA
VAVLIVTLLYTTVGGFRSASHVNVLQGAIMLLATCILPLSLLIKFGLEPLTTFLKQSGSSAVGHLDITQAVFYFSFASVM
FGTPHTIGKFLAIKSPDKLNTARNTSIIINIIIYLGIAISAVYGRKLLPSMKDTEMLLLNLSEMFTSPLYGSMIIIAILA
AVMSTIDAQLISSASCIANDIYRHITSNRNDIHLIFATKLMIIFVGALALSISLIFNQSVGKLAIFSLTGLGASIGPVIL
FSLYTKTRNKIPALLGLTSGTTATILLTIFNTSDIHYNMLPALCITAVVMLASSCITGRTVKCVESNN
>Mature_468_residues
MFYTHLLVVGLYLLCIFLFGFVRKRVSASFKEYILANRSVHEIITGISHVSSEISVGTFTVVASMIYTTGFSRLWVDLGF
FVFSILSWRLLAGRLRVQSEESKDALTIPQYLANKFDSKTVGIFASLIITLFTTTYIGANMLGLTKVLSKVVSLESNLVA
VAVLIVTLLYTTVGGFRSASHVNVLQGAIMLLATCILPLSLLIKFGLEPLTTFLKQSGSSAVGHLDITQAVFYFSFASVM
FGTPHTIGKFLAIKSPDKLNTARNTSIIINIIIYLGIAISAVYGRKLLPSMKDTEMLLLNLSEMFTSPLYGSMIIIAILA
AVMSTIDAQLISSASCIANDIYRHITSNRNDIHLIFATKLMIIFVGALALSISLIFNQSVGKLAIFSLTGLGASIGPVIL
FSLYTKTRNKIPALLGLTSGTTATILLTIFNTSDIHYNMLPALCITAVVMLASSCITGRTVKCVESNN

Specific function: Catalyzes the sodium-dependent uptake of extracellular proline [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=490, Percent_Identity=27.1428571428571, Blast_Score=178, Evalue=1e-44,
Organism=Escherichia coli, GI1787251, Length=451, Percent_Identity=25.9423503325942, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI87082237, Length=342, Percent_Identity=24.5614035087719, Blast_Score=86, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 50789; Mature: 50789

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
>Mature Secondary Structure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CCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377 [H]