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The map label for this gene is purA

Identifier: 88608367

GI number: 88608367

Start: 191077

End: 192330

Strand: Direct

Name: purA

Synonym: NSE_0225

Alternate gene names: 88608367

Gene position: 191077-192330 (Clockwise)

Preceding gene: 88608439

Following gene: 88608176

Centisome position: 22.24

GC content: 42.9

Gene sequence:

>1254_bases
ATGGACGCTGCAGTTATAGGGTTACAGTGGGGTGATGAAGGTAAAGGTAAAATCGTAGATCATCTTGCTGGCGACTTCGA
CAGTGTTGTCCGTTTTAACGGTGGAAATAATGCTGGGCATACAGTAATTGTAGGCGAGAGAACCTATAAGCTTAGGGTCC
TACCATCAGGCATTTTGAGGGAGGATGTTGTTTCCGTCATTGGTAATGGAGTTGTTCTTGACCCACAAGGTCTACTAGAT
GAAATTCATCTGCTAAAGAAAGAAGGCGTCCAAATACACCCAGGTAAGCTGTTGATTGCCGATAACTGTCATCTTGTTCT
TCCTATACATAGGGAGTTGGATGTTCTATTCGAGCAAAAACAGAAACTAGGGACCACTAAATGTGGTATTGGTCCATGTT
ACCAGGATAAAGTTGCTAGGCGAGGTATACGCTTGTGTGATTTACGATCTGAAGAGCAATTAAGAAAGACTTTGGCAGGG
CTTTCATACTATCATAACATCGTACGGAAAGGGGCTGGGCTGCAGGAAGTTTGTCAGGAAATCCTGTTTGCATCGCTCTT
TGCAATGAGGGACGAGCTTCTTTGTTATGCAGTTCCTCCATGTGAACTGCAGGGCATTTTGTCCGGTAAAAGTAAGCTTT
ACGAAGGTGCTCAGGGAATGCTACTCGACATAGATCATGGTACGTATCCATTTGTAACGTCAGGTTCTTCGGGTTTGGGT
CAGGTTATGAACGGTGCTGCACTTGGCTGTGTGAATAGGGTGATAGGGGTCATGAAGGGTTATGTTACCCGCGTTGGAGA
GGGTATTCTTCCGACTGAACAGGATAATGCATTTGGTAGCGAGTTACAGAAACTGGGAAAGGAAGTTGGTACGGTAAGTG
GAAGGATCAGAAGATGTGGTTGGTGTGACCTTCCCTTAGTTAGGTATGTCAATGCTGTTGCGGGGGTAACGGAAATTGTT
ATAACGAAACTTGATGTTTTAGACTCTTTTAATGAAATCTTTTTATGTTGTGCTTACAAAGGTAAAAAAGGTAAGTTGAT
TGAAATTTGTAGTCCTAGTAAACTGTGCTATAGCAAGTATGAAGCGCAGTACATAGTAATGAAAGGGTGGAGATCGTCAA
CTGTTGGTGTTACTTCTTTTTGTGATCTCCCCGATGGAGCAAAAAGTTTTGTGGAGACAATTGAAAAATGTCTTGATCTT
CCAGTTACTATGATTTCTAATGGGCCTGAGAGGACCCAAGTTCTTCATAGGTAA

Upstream 100 bases:

>100_bases
TGGGACTGCTTAAATTTCCTGTTTATCCTATCTTCTAAGCTTTTCTTACTATATTCCCTCATGTTACAATCCGGGGTCTT
CGGTGTTTTTGGAGAGCTGT

Downstream 100 bases:

>100_bases
TAATGAAAGTCAGTGTCGTTGTTTTGTCCACAGCGAGAAGATAAATAGTGGTTTCTTTGTGAGTGTCCTAGAGTAGAGAT
CGCGGTCTTATGTTTAGTCT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MDAAVIGLQWGDEGKGKIVDHLAGDFDSVVRFNGGNNAGHTVIVGERTYKLRVLPSGILREDVVSVIGNGVVLDPQGLLD
EIHLLKKEGVQIHPGKLLIADNCHLVLPIHRELDVLFEQKQKLGTTKCGIGPCYQDKVARRGIRLCDLRSEEQLRKTLAG
LSYYHNIVRKGAGLQEVCQEILFASLFAMRDELLCYAVPPCELQGILSGKSKLYEGAQGMLLDIDHGTYPFVTSGSSGLG
QVMNGAALGCVNRVIGVMKGYVTRVGEGILPTEQDNAFGSELQKLGKEVGTVSGRIRRCGWCDLPLVRYVNAVAGVTEIV
ITKLDVLDSFNEIFLCCAYKGKKGKLIEICSPSKLCYSKYEAQYIVMKGWRSSTVGVTSFCDLPDGAKSFVETIEKCLDL
PVTMISNGPERTQVLHR

Sequences:

>Translated_417_residues
MDAAVIGLQWGDEGKGKIVDHLAGDFDSVVRFNGGNNAGHTVIVGERTYKLRVLPSGILREDVVSVIGNGVVLDPQGLLD
EIHLLKKEGVQIHPGKLLIADNCHLVLPIHRELDVLFEQKQKLGTTKCGIGPCYQDKVARRGIRLCDLRSEEQLRKTLAG
LSYYHNIVRKGAGLQEVCQEILFASLFAMRDELLCYAVPPCELQGILSGKSKLYEGAQGMLLDIDHGTYPFVTSGSSGLG
QVMNGAALGCVNRVIGVMKGYVTRVGEGILPTEQDNAFGSELQKLGKEVGTVSGRIRRCGWCDLPLVRYVNAVAGVTEIV
ITKLDVLDSFNEIFLCCAYKGKKGKLIEICSPSKLCYSKYEAQYIVMKGWRSSTVGVTSFCDLPDGAKSFVETIEKCLDL
PVTMISNGPERTQVLHR
>Mature_417_residues
MDAAVIGLQWGDEGKGKIVDHLAGDFDSVVRFNGGNNAGHTVIVGERTYKLRVLPSGILREDVVSVIGNGVVLDPQGLLD
EIHLLKKEGVQIHPGKLLIADNCHLVLPIHRELDVLFEQKQKLGTTKCGIGPCYQDKVARRGIRLCDLRSEEQLRKTLAG
LSYYHNIVRKGAGLQEVCQEILFASLFAMRDELLCYAVPPCELQGILSGKSKLYEGAQGMLLDIDHGTYPFVTSGSSGLG
QVMNGAALGCVNRVIGVMKGYVTRVGEGILPTEQDNAFGSELQKLGKEVGTVSGRIRRCGWCDLPLVRYVNAVAGVTEIV
ITKLDVLDSFNEIFLCCAYKGKKGKLIEICSPSKLCYSKYEAQYIVMKGWRSSTVGVTSFCDLPDGAKSFVETIEKCLDL
PVTMISNGPERTQVLHR

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI34577063, Length=435, Percent_Identity=40.4597701149425, Blast_Score=286, Evalue=2e-77,
Organism=Homo sapiens, GI22748717, Length=433, Percent_Identity=39.0300230946882, Blast_Score=283, Evalue=2e-76,
Organism=Homo sapiens, GI40316944, Length=404, Percent_Identity=37.3762376237624, Blast_Score=247, Evalue=1e-65,
Organism=Escherichia coli, GI1790620, Length=423, Percent_Identity=41.371158392435, Blast_Score=301, Evalue=4e-83,
Organism=Caenorhabditis elegans, GI25146553, Length=432, Percent_Identity=37.7314814814815, Blast_Score=264, Evalue=8e-71,
Organism=Caenorhabditis elegans, GI25146555, Length=432, Percent_Identity=37.7314814814815, Blast_Score=263, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6324109, Length=431, Percent_Identity=37.122969837587, Blast_Score=285, Evalue=1e-77,
Organism=Drosophila melanogaster, GI21356033, Length=426, Percent_Identity=41.3145539906103, Blast_Score=290, Evalue=1e-78,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_NEOSM (Q2GEH7)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506119.1
- ProteinModelPortal:   Q2GEH7
- SMR:   Q2GEH7
- STRING:   Q2GEH7
- GeneID:   3931531
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0225
- TIGR:   NSE_0225
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- ProtClustDB:   CLSK2602730
- BioCyc:   NSEN222891:NSE_0225-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 45534; Mature: 45534

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 12-12 ACT_SITE 40-40 BINDING 126-126 BINDING 140-140 BINDING 218-218 BINDING 233-233 BINDING 295-295 BINDING 297-297

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAAVIGLQWGDEGKGKIVDHLAGDFDSVVRFNGGNNAGHTVIVGERTYKLRVLPSGILR
CCCEEEEEEECCCCCCCHHHHHHCCHHHEEEECCCCCCCCEEEEECCEEEEEECCCHHHH
EDVVSVIGNGVVLDPQGLLDEIHLLKKEGVQIHPGKLLIADNCHLVLPIHRELDVLFEQK
HHHHHHHCCCEEECCHHHHHHHHHHHHCCCEECCCEEEEECCCEEEEECHHHHHHHHHHH
QKLGTTKCGIGPCYQDKVARRGIRLCDLRSEEQLRKTLAGLSYYHNIVRKGAGLQEVCQE
HHCCCCCCCCCCCHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ILFASLFAMRDELLCYAVPPCELQGILSGKSKLYEGAQGMLLDIDHGTYPFVTSGSSGLG
HHHHHHHHHHHCEEEEECCCHHHHHHHCCHHHHHCCCCCEEEEECCCCCCEEECCCCCHH
QVMNGAALGCVNRVIGVMKGYVTRVGEGILPTEQDNAFGSELQKLGKEVGTVSGRIRRCG
HHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
WCDLPLVRYVNAVAGVTEIVITKLDVLDSFNEIFLCCAYKGKKGKLIEICSPSKLCYSKY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEECCCCHHHHHHH
EAQYIVMKGWRSSTVGVTSFCDLPDGAKSFVETIEKCLDLPVTMISNGPERTQVLHR
CCEEEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCEEECCCCCHHHHHCCC
>Mature Secondary Structure
MDAAVIGLQWGDEGKGKIVDHLAGDFDSVVRFNGGNNAGHTVIVGERTYKLRVLPSGILR
CCCEEEEEEECCCCCCCHHHHHHCCHHHEEEECCCCCCCCEEEEECCEEEEEECCCHHHH
EDVVSVIGNGVVLDPQGLLDEIHLLKKEGVQIHPGKLLIADNCHLVLPIHRELDVLFEQK
HHHHHHHCCCEEECCHHHHHHHHHHHHCCCEECCCEEEEECCCEEEEECHHHHHHHHHHH
QKLGTTKCGIGPCYQDKVARRGIRLCDLRSEEQLRKTLAGLSYYHNIVRKGAGLQEVCQE
HHCCCCCCCCCCCHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ILFASLFAMRDELLCYAVPPCELQGILSGKSKLYEGAQGMLLDIDHGTYPFVTSGSSGLG
HHHHHHHHHHHCEEEEECCCHHHHHHHCCHHHHHCCCCCEEEEECCCCCCEEECCCCCHH
QVMNGAALGCVNRVIGVMKGYVTRVGEGILPTEQDNAFGSELQKLGKEVGTVSGRIRRCG
HHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
WCDLPLVRYVNAVAGVTEIVITKLDVLDSFNEIFLCCAYKGKKGKLIEICSPSKLCYSKY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEECCCCHHHHHHH
EAQYIVMKGWRSSTVGVTSFCDLPDGAKSFVETIEKCLDLPVTMISNGPERTQVLHR
CCEEEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCEEECCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA