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The map label for this gene is fucA [C]

Identifier: 88608359

GI number: 88608359

Start: 751546

End: 752280

Strand: Direct

Name: fucA [C]

Synonym: NSE_0849

Alternate gene names: 88608359

Gene position: 751546-752280 (Clockwise)

Preceding gene: 88608479

Following gene: 88608008

Centisome position: 87.49

GC content: 42.45

Gene sequence:

>735_bases
ATGCCTGATCTTCCTATCAAGAAAGACCTTTTGTACGCATACCGAATTATCGGCTATCTTGGTATGGATGATCTCACCTA
TACTCACATCACTGTCCGCCCAGAGAATGCAGATTTCTTTTACATAGCAAAGTTTGGCGTTCTTTTTAATCAGGTCACTT
TCGGTGATTTAGTCAAAGTCAGTTTGAACGGCGAAATTCTCGATGGAGAAGAGGTCGTATACAATAAAACAGCCTACACA
ATACATGGCTCCATATATAACAGCCGTAGTGACGTGAATGCGGTATACCATTTGCACACACAGGCAAGTATTGCGGTCTC
CGTTATGGAGTGTGGACTCTTACCACTTTCCCAGTTTGCGATGCCCTTTTTTGAGAACATCTCCTATTACAAATATGACT
CTCTTGCTTTGGATAAAGCGACTCAAGGAGCGGATCTAGTGAATAGCCTAGGTTCTTACAAAGCAATGCTGATGCGTTCA
CATGGTTTGCTAACATGCGGTAGTAACCTACAAGAGGCTCTTTTCTATACCAGATTTCTAGAGCAAGCTTGTAGAACGCA
AGTTGCAATTCTTTCCACAAAACAGAAATACATACTTCCAGGACCGGATGTATGTAGAAAGGCAAGGCAGGATATGCTTA
CATTTGAGGAGAGATTAGGCGAACGCGATTGGAAAGCTCTCTCGAAGTTATTTGAAAAAGGATCTGACCCATTGAGTCTA
TGTCTGCAGTCCTAG

Upstream 100 bases:

>100_bases
GGTGTAACAAAGAAAAAACAGTGATAATGGCATGAACGAGTTAGGTGCCTGCTTGCGCTGTGTGATGCGTTCACCCGTGC
GTGGTTTTATTTGTTAATCT

Downstream 100 bases:

>100_bases
GATTTTCAAGGAAATTTCCGGTAAAGATAGTACGAGCCAAAGGTATATATCTTTTCGACTCCAATGGAAAACAGTATTGT
GACTTCACAAGTGGTATTGC

Product: hypothetical protein

Products: Dihydroxyacetone phosphate; L-Lactaldehyde [C]

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 243

Protein sequence:

>244_residues
MPDLPIKKDLLYAYRIIGYLGMDDLTYTHITVRPENADFFYIAKFGVLFNQVTFGDLVKVSLNGEILDGEEVVYNKTAYT
IHGSIYNSRSDVNAVYHLHTQASIAVSVMECGLLPLSQFAMPFFENISYYKYDSLALDKATQGADLVNSLGSYKAMLMRS
HGLLTCGSNLQEALFYTRFLEQACRTQVAILSTKQKYILPGPDVCRKARQDMLTFEERLGERDWKALSKLFEKGSDPLSL
CLQS

Sequences:

>Translated_244_residues
MPDLPIKKDLLYAYRIIGYLGMDDLTYTHITVRPENADFFYIAKFGVLFNQVTFGDLVKVSLNGEILDGEEVVYNKTAYT
IHGSIYNSRSDVNAVYHLHTQASIAVSVMECGLLPLSQFAMPFFENISYYKYDSLALDKATQGADLVNSLGSYKAMLMRS
HGLLTCGSNLQEALFYTRFLEQACRTQVAILSTKQKYILPGPDVCRKARQDMLTFEERLGERDWKALSKLFEKGSDPLSL
CLQS
>Mature_243_residues
PDLPIKKDLLYAYRIIGYLGMDDLTYTHITVRPENADFFYIAKFGVLFNQVTFGDLVKVSLNGEILDGEEVVYNKTAYTI
HGSIYNSRSDVNAVYHLHTQASIAVSVMECGLLPLSQFAMPFFENISYYKYDSLALDKATQGADLVNSLGSYKAMLMRSH
GLLTCGSNLQEALFYTRFLEQACRTQVAILSTKQKYILPGPDVCRKARQDMLTFEERLGERDWKALSKLFEKGSDPLSLC
LQS

Specific function: Fucose metabolism; third step. [C]

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family [H]

Homologues:

Organism=Homo sapiens, GI29826325, Length=193, Percent_Identity=36.2694300518135, Blast_Score=127, Evalue=8e-30,
Organism=Homo sapiens, GI29826321, Length=193, Percent_Identity=36.2694300518135, Blast_Score=127, Evalue=8e-30,
Organism=Homo sapiens, GI29826323, Length=193, Percent_Identity=36.2694300518135, Blast_Score=127, Evalue=8e-30,
Organism=Homo sapiens, GI29826319, Length=193, Percent_Identity=36.2694300518135, Blast_Score=127, Evalue=9e-30,
Organism=Homo sapiens, GI9943848, Length=183, Percent_Identity=34.9726775956284, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI9951927, Length=183, Percent_Identity=34.9726775956284, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI62912453, Length=183, Percent_Identity=34.9726775956284, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI297307160, Length=187, Percent_Identity=33.6898395721925, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI9257190, Length=187, Percent_Identity=33.6898395721925, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI89276787, Length=187, Percent_Identity=33.6898395721925, Blast_Score=106, Evalue=1e-23,
Organism=Homo sapiens, GI297307158, Length=187, Percent_Identity=33.6898395721925, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI9257192, Length=187, Percent_Identity=33.6898395721925, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI115533218, Length=243, Percent_Identity=31.6872427983539, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI115533216, Length=189, Percent_Identity=35.978835978836, Blast_Score=125, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71989541, Length=222, Percent_Identity=27.4774774774775, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71989555, Length=222, Percent_Identity=27.4774774774775, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71989560, Length=222, Percent_Identity=27.4774774774775, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71989550, Length=222, Percent_Identity=27.4774774774775, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71989544, Length=222, Percent_Identity=27.4774774774775, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI281363755, Length=203, Percent_Identity=28.5714285714286, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281363751, Length=203, Percent_Identity=28.5714285714286, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24655690, Length=203, Percent_Identity=28.5714285714286, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24655685, Length=203, Percent_Identity=28.5714285714286, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI281363761, Length=203, Percent_Identity=28.5714285714286, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI281363759, Length=203, Percent_Identity=28.5714285714286, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI281363753, Length=203, Percent_Identity=28.5714285714286, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI281363763, Length=203, Percent_Identity=28.5714285714286, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI281363757, Length=203, Percent_Identity=28.5714285714286, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI17136738, Length=203, Percent_Identity=28.5714285714286, Blast_Score=88, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303 [H]

Pfam domain/function: PF00596 Aldolase_II [H]

EC number: 4.1.2.17 [C]

Molecular weight: Translated: 27568; Mature: 27437

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDLPIKKDLLYAYRIIGYLGMDDLTYTHITVRPENADFFYIAKFGVLFNQVTFGDLVKV
CCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCEEEEEHHHHHHHHCCCCCEEEE
SLNGEILDGEEVVYNKTAYTIHGSIYNSRSDVNAVYHLHTQASIAVSVMECGLLPLSQFA
EECCEEECCCEEEEECEEEEEEEEEECCCCCCEEEEEEECHHHHHHHHHHHCCCCHHHHH
MPFFENISYYKYDSLALDKATQGADLVNSLGSYKAMLMRSHGLLTCGSNLQEALFYTRFL
HHHHHCCCEEEECCEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHH
EQACRTQVAILSTKQKYILPGPDVCRKARQDMLTFEERLGERDWKALSKLFEKGSDPLSL
HHHHHHHHHEEECCCCEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHH
CLQS
HCCC
>Mature Secondary Structure 
PDLPIKKDLLYAYRIIGYLGMDDLTYTHITVRPENADFFYIAKFGVLFNQVTFGDLVKV
CCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCEEEEEHHHHHHHHCCCCCEEEE
SLNGEILDGEEVVYNKTAYTIHGSIYNSRSDVNAVYHLHTQASIAVSVMECGLLPLSQFA
EECCEEECCCEEEEECEEEEEEEEEECCCCCCEEEEEEECHHHHHHHHHHHCCCCHHHHH
MPFFENISYYKYDSLALDKATQGADLVNSLGSYKAMLMRSHGLLTCGSNLQEALFYTRFL
HHHHHCCCEEEECCEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHH
EQACRTQVAILSTKQKYILPGPDVCRKARQDMLTFEERLGERDWKALSKLFEKGSDPLSL
HHHHHHHHHEEECCCCEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHH
CLQS
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+; Co2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): 19.3 [C]

Specific activity: 21.0

Km value (mM): 0.7 {L-fuculose} [C]

Substrates: L-Fuculose 1-phosphate [C]

Specific reaction: L-Fuculose 1-phosphate <==> Dihydroxyacetone phosphate + L-Lactaldehyde [C]

General reaction: Elimination of an aldehyde C-C bond; Cleavage [C]

Inhibitor: EDTA; Phosphoglycolohydroxamate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]