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The map label for this gene is tuf

Identifier: 88608355

GI number: 88608355

Start: 605262

End: 606554

Strand: Reverse

Name: tuf

Synonym: NSE_0686

Alternate gene names: 88608355

Gene position: 606554-605262 (Counterclockwise)

Preceding gene: 88608233

Following gene: 88608140

Centisome position: 70.61

GC content: 46.09

Gene sequence:

>1293_bases
ATGGCTGTTAAACAGAAGCAGGTCTTCGTCAACGATAGACCGCATTTAAATATAGGTACGATAGGGCACGTTGATCATGG
AAAGACGACCTTGACTGCTGCGATTACCAAGTTTTGTTCAGAGGAAGGTGGTGGCTATGAGGCTGATTTTAGGGCGTATG
ATAATATTGATAAGGCCCCAGAAGAGAGGCAGCGTGGTATTACCATTTCCACTGCGCACGTTGAGTATAAGACTCCGGAG
CGTCACTATGCACACGTGGACTGTCCGGGACACGCGGATTATATCAAGAATATGATCACGGGTGCTGCACAAATGGACGG
TGCTATACTTGTGGTTGCGGGTACAGATGGTGCCATGCAGCAAACAAAGGAGCACATCCTTTTAGCAAAGCAGGTTGGTG
TCGGGAGTATAGTTGTTTACATCAACAAGTGTGATTCTTCTGAGCTTGATGAGGAGCTTCTGGAGCTTGTTGAGAGTGAT
ATCAAGGATCTTTTGATATCACATGGATTCGATTTGCCAGAGGATGAGGAAGATGGAAGTAATCCGGCAATCATAAGGGG
TTCCGCACTATTGGCGCTTAATGGCGAGGAAAGTGAGCTTGGCAAGGGTTCTATTCGCAAGTTGCTTGCTGCGTGCGATA
AGTATATTGCGCTTCCTGAAAGGGCGGTTGATGGTGATTTTCTCATGTCTATTGAGGATGTCTTTTCCATTTCCGGACGT
GGAACTGTTGTTACAGGAAAAATTGAGCGGGGCTGTATCAAAGTTGGTGATGGGGTTGAGATTGTTGGTATAAGGGATAC
ACAAAAAACTACCTGTACTGGAGTCGAAATGTTTAACAAGCTTGTTGAACAAGGTGAGGCTGGATTTAATGTTGGTATCC
TTCTCCGTGGTTCGAAAAGAGAAGATGTATGTAGGGGACAAGTTCTCTGTAAGCCGGGTTCGATTACTCCTCATAGGAAG
TTGAGGGCGAGGATCGTGACTCTTACAAAAGAGGAAGGTGGCAGAAGGACAGGGTTCGTGTCTGGGTATAAGCCGCAGTT
TTATTTCCGTACGACAGATGTTACTGGCACGGCGTACTTGCCTGTAGATGGGGCTGAAATTGTCATGCCAGGTGATGATT
TGGAGATTTTTGTGGAGCTCTTGAATCCGATTGCGATGGAGAAAGGGTCTAGGTTTGCAATCAGAGAGGGGGGAGTGACA
GTAGGCGCTGGTCAAGTGTTGGAGATTATGGATTCTAAGACTGATGCCGAAATAGCAAAGATGTTCAAAATTGTGAGAGC
TGTGAAGAATTGA

Upstream 100 bases:

>100_bases
GTTCAAAGAATATGCAAAAATGCCCGACCATATAGCTGCTGAGCTGGTTGACAAGCGTGTTGCTAATTAGATGTTGTAAA
GATAATAAGGAGAAGTAGAA

Downstream 100 bases:

>100_bases
GTCTTAGGAGCGTAGCTCAATTGGTAGAGTTTCGGTCTCCAAAACCGATGGTTGTAGGTTCGAGTCCTATCGCTCCTGTT
CTTATGTGTATGTTTTGGAT

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MAVKQKQVFVNDRPHLNIGTIGHVDHGKTTLTAAITKFCSEEGGGYEADFRAYDNIDKAPEERQRGITISTAHVEYKTPE
RHYAHVDCPGHADYIKNMITGAAQMDGAILVVAGTDGAMQQTKEHILLAKQVGVGSIVVYINKCDSSELDEELLELVESD
IKDLLISHGFDLPEDEEDGSNPAIIRGSALLALNGEESELGKGSIRKLLAACDKYIALPERAVDGDFLMSIEDVFSISGR
GTVVTGKIERGCIKVGDGVEIVGIRDTQKTTCTGVEMFNKLVEQGEAGFNVGILLRGSKREDVCRGQVLCKPGSITPHRK
LRARIVTLTKEEGGRRTGFVSGYKPQFYFRTTDVTGTAYLPVDGAEIVMPGDDLEIFVELLNPIAMEKGSRFAIREGGVT
VGAGQVLEIMDSKTDAEIAKMFKIVRAVKN

Sequences:

>Translated_430_residues
MAVKQKQVFVNDRPHLNIGTIGHVDHGKTTLTAAITKFCSEEGGGYEADFRAYDNIDKAPEERQRGITISTAHVEYKTPE
RHYAHVDCPGHADYIKNMITGAAQMDGAILVVAGTDGAMQQTKEHILLAKQVGVGSIVVYINKCDSSELDEELLELVESD
IKDLLISHGFDLPEDEEDGSNPAIIRGSALLALNGEESELGKGSIRKLLAACDKYIALPERAVDGDFLMSIEDVFSISGR
GTVVTGKIERGCIKVGDGVEIVGIRDTQKTTCTGVEMFNKLVEQGEAGFNVGILLRGSKREDVCRGQVLCKPGSITPHRK
LRARIVTLTKEEGGRRTGFVSGYKPQFYFRTTDVTGTAYLPVDGAEIVMPGDDLEIFVELLNPIAMEKGSRFAIREGGVT
VGAGQVLEIMDSKTDAEIAKMFKIVRAVKN
>Mature_429_residues
AVKQKQVFVNDRPHLNIGTIGHVDHGKTTLTAAITKFCSEEGGGYEADFRAYDNIDKAPEERQRGITISTAHVEYKTPER
HYAHVDCPGHADYIKNMITGAAQMDGAILVVAGTDGAMQQTKEHILLAKQVGVGSIVVYINKCDSSELDEELLELVESDI
KDLLISHGFDLPEDEEDGSNPAIIRGSALLALNGEESELGKGSIRKLLAACDKYIALPERAVDGDFLMSIEDVFSISGRG
TVVTGKIERGCIKVGDGVEIVGIRDTQKTTCTGVEMFNKLVEQGEAGFNVGILLRGSKREDVCRGQVLCKPGSITPHRKL
RARIVTLTKEEGGRRTGFVSGYKPQFYFRTTDVTGTAYLPVDGAEIVMPGDDLEIFVELLNPIAMEKGSRFAIREGGVTV
GAGQVLEIMDSKTDAEIAKMFKIVRAVKN

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=406, Percent_Identity=53.2019704433498, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI223555963, Length=432, Percent_Identity=28.0092592592593, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI5729864, Length=432, Percent_Identity=28.0092592592593, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI4503471, Length=331, Percent_Identity=32.3262839879154, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI4503475, Length=339, Percent_Identity=31.2684365781711, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI194018520, Length=350, Percent_Identity=28.2857142857143, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI194097354, Length=350, Percent_Identity=28.2857142857143, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI194018522, Length=350, Percent_Identity=28.2857142857143, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI46094014, Length=336, Percent_Identity=27.9761904761905, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI54607086, Length=328, Percent_Identity=28.9634146341463, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI157426893, Length=301, Percent_Identity=27.2425249169435, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI53729339, Length=245, Percent_Identity=30.6122448979592, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI53729337, Length=245, Percent_Identity=30.6122448979592, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1790412, Length=406, Percent_Identity=61.0837438423645, Blast_Score=480, Evalue=1e-137,
Organism=Escherichia coli, GI1789737, Length=406, Percent_Identity=61.0837438423645, Blast_Score=480, Evalue=1e-137,
Organism=Escherichia coli, GI2367247, Length=358, Percent_Identity=29.0502793296089, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI48994988, Length=246, Percent_Identity=30.8943089430894, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1789108, Length=440, Percent_Identity=24.5454545454545, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1788922, Length=141, Percent_Identity=32.6241134751773, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=420, Percent_Identity=47.8571428571429, Blast_Score=356, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI25141371, Length=399, Percent_Identity=41.8546365914787, Blast_Score=299, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI17552884, Length=345, Percent_Identity=32.463768115942, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17569207, Length=345, Percent_Identity=32.463768115942, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI115532067, Length=353, Percent_Identity=30.028328611898, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI115532065, Length=353, Percent_Identity=30.028328611898, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI32566629, Length=453, Percent_Identity=25.60706401766, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI32566303, Length=321, Percent_Identity=31.4641744548287, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17506081, Length=287, Percent_Identity=28.5714285714286, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI32566301, Length=146, Percent_Identity=39.7260273972603, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17557151, Length=244, Percent_Identity=27.0491803278689, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=407, Percent_Identity=57.4938574938575, Blast_Score=454, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6325337, Length=447, Percent_Identity=29.7539149888143, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6319594, Length=447, Percent_Identity=29.7539149888143, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6322937, Length=278, Percent_Identity=28.0575539568345, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6320377, Length=323, Percent_Identity=28.1733746130031, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=249, Percent_Identity=29.718875502008, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281363316, Length=417, Percent_Identity=49.8800959232614, Blast_Score=420, Evalue=1e-118,
Organism=Drosophila melanogaster, GI17864358, Length=417, Percent_Identity=49.8800959232614, Blast_Score=420, Evalue=1e-118,
Organism=Drosophila melanogaster, GI19921738, Length=403, Percent_Identity=47.8908188585608, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI45553807, Length=469, Percent_Identity=30.0639658848614, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI45553816, Length=469, Percent_Identity=30.0639658848614, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24651721, Length=469, Percent_Identity=30.0639658848614, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI17864154, Length=469, Percent_Identity=30.0639658848614, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24652838, Length=323, Percent_Identity=32.1981424148607, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI17137572, Length=323, Percent_Identity=32.1981424148607, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI45550900, Length=434, Percent_Identity=27.1889400921659, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI17137380, Length=333, Percent_Identity=31.8318318318318, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI19922690, Length=313, Percent_Identity=27.7955271565495, Blast_Score=101, Evalue=9e-22,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_NEOSM (Q2GD83)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506563.1
- HSSP:   P0A6N1
- STRING:   Q2GD83
- GeneID:   3931520
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0686
- TIGR:   NSE_0686
- eggNOG:   COG0050
- HOGENOM:   HBG307581
- OMA:   RDKPFLM
- ProtClustDB:   PRK00049
- BioCyc:   NSEN222891:NSE_0686-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 46706; Mature: 46575

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVKQKQVFVNDRPHLNIGTIGHVDHGKTTLTAAITKFCSEEGGGYEADFRAYDNIDKAP
CCCCCCEEEECCCCCEEECCEECCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCH
EERQRGITISTAHVEYKTPERHYAHVDCPGHADYIKNMITGAAQMDGAILVVAGTDGAMQ
HHHHCCCEEEEEEEEECCCCCCEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCHHHH
QTKEHILLAKQVGVGSIVVYINKCDSSELDEELLELVESDIKDLLISHGFDLPEDEEDGS
HHHHHEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
NPAIIRGSALLALNGEESELGKGSIRKLLAACDKYIALPERAVDGDFLMSIEDVFSISGR
CCEEEECCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHEEEHHHHHCCCCC
GTVVTGKIERGCIKVGDGVEIVGIRDTQKTTCTGVEMFNKLVEQGEAGFNVGILLRGSKR
CEEEEECCCCCEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCC
EDVCRGQVLCKPGSITPHRKLRARIVTLTKEEGGRRTGFVSGYKPQFYFRTTDVTGTAYL
CCCCCCCEEECCCCCCCCHHEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEEE
PVDGAEIVMPGDDLEIFVELLNPIAMEKGSRFAIREGGVTVGAGQVLEIMDSKTDAEIAK
ECCCCEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCEEECCCHHHHHHCCCCHHHHHH
MFKIVRAVKN
HHHHHHHHCC
>Mature Secondary Structure 
AVKQKQVFVNDRPHLNIGTIGHVDHGKTTLTAAITKFCSEEGGGYEADFRAYDNIDKAP
CCCCCEEEECCCCCEEECCEECCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCH
EERQRGITISTAHVEYKTPERHYAHVDCPGHADYIKNMITGAAQMDGAILVVAGTDGAMQ
HHHHCCCEEEEEEEEECCCCCCEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCHHHH
QTKEHILLAKQVGVGSIVVYINKCDSSELDEELLELVESDIKDLLISHGFDLPEDEEDGS
HHHHHEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
NPAIIRGSALLALNGEESELGKGSIRKLLAACDKYIALPERAVDGDFLMSIEDVFSISGR
CCEEEECCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHEEEHHHHHCCCCC
GTVVTGKIERGCIKVGDGVEIVGIRDTQKTTCTGVEMFNKLVEQGEAGFNVGILLRGSKR
CEEEEECCCCCEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCC
EDVCRGQVLCKPGSITPHRKLRARIVTLTKEEGGRRTGFVSGYKPQFYFRTTDVTGTAYL
CCCCCCCEEECCCCCCCCHHEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEEE
PVDGAEIVMPGDDLEIFVELLNPIAMEKGSRFAIREGGVTVGAGQVLEIMDSKTDAEIAK
ECCCCEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCEEECCCHHHHHHCCCCHHHHHH
MFKIVRAVKN
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA