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The map label for this gene is gidA

Identifier: 88608296

GI number: 88608296

Start: 626414

End: 628297

Strand: Reverse

Name: gidA

Synonym: NSE_0707

Alternate gene names: 88608296

Gene position: 628297-626414 (Counterclockwise)

Preceding gene: 88608826

Following gene: 88608388

Centisome position: 73.14

GC content: 43.15

Gene sequence:

>1884_bases
ATGCAATACGTTGTCGTGATCGGCGGGGGTCATGCTGGAGTTGAAGCTGCTGCTGCATCTGCAAGGCTTGGTGTTGAAAC
GCTCCTTGTGACAAACAATGTAATGAACATTGGTGAGATGTCCTGCAATCCTGCTATAGGTGGTATAGGGAAGGGTAATG
TTGTCAAAGAAGTAGATGCCATGGGGGGCGTCATGGCTCTGGCGATAGATAGAGCCAGTATACATTCTAGGATGCTTAAT
CGTAGTAAAGGGGCTGCTGTTTGGGGCCCTCGTGCTCAGGCTGATAGGAAACTTTACAAGTGTGCGGTACTTGAGCTGCT
TACTAAATACGAGAAGCTCAGTATTCTTGAAGACCATATCACTGATCTTATTATTGAAAATGATCGTCTTATGGGTGTGG
TTGGAGAAAAAACTGGAACTATCAAATGTAGTGCGGCTGTCTTAACTACCGGGACTTTCTTAAATGGTATCATTCAGACC
GGTAGTGAAAGAGTCGAAGGTGGTAGGTTCGGTGAAAAGGCATCCCAGTGCTTGGGAAATACATTAAGGCGACATTTCAA
AATTTCTAGGTTAAGAACAGGAACCCCGGCGCGTCTTTACAAAGACTCTATCAATTATTCTGCGCTTGTTGAGCAGCCAG
GTGATTCGCCGCCTATACCGTTCTCATATATGAATACTGAAATAACGGTACCACAGGTTTCTTGCTATATAACTCATACA
AATGGTAAGACTCACGAGATAATAAGAAATTCACTTAAATTTTCTGCAATTAGAAACGGTGTATCTGCTAGGGGGCCACG
CTATTGTCCATCTATCGAGGATAAGGTTGTCCGTTTTGCTGAGAAAGATAGCCATCAAATTTTCCTAGAGCCAGAAGGTT
TGGATTCAGAGCTTGTATATCCAAATGGGATCTCCAACTCACTCCCTAAGGAGATCCAGGAAGAATTTATTCGCAGTATC
GCGGGTCTTGAAAAAGCAAGTGTGGCAAGGTACGCGTATACGATAGAATACGACTATATTGATCCAAGAGAATTGCGTTC
TACCTTGGAGAGTAAGCGGGTAAAAAATCTCTATTTTGCTGGTCAGATAAATGGAACGACAGGTTATGAGGAAGCGGCTG
GTCAGGGTATTGTTGCTGGAAGTAATGCCGCGGGTGCAGGTCTGATAATTAGTAGATCTGAGGGTTATATTGGTGTGATG
ATAGATGATCTCATCACGCTTGGTACCAACGGTGAACCATATAGGTTATTTACCTCCAGGGCAGAGTATCGGCTTAATCT
GCGTTCTGATAACGCGGATTTTCGACTCACTGAAAAAGCATATAGAGTAGGTTTGGTCGATGAGGAACGCTACGCAGCGT
ACAAGAAGAAATACGACACTTTTCATAACTACAAAAGTAAGCTCAATGAGCTTTCAACCACTCCTTACGAACTAGCAAAG
ATTGAAGGTATTTCAATCGCTCAGGATGGAGTAAGGAAAAGTGCGTGGAATCTTATAACACAACCGCTTTTTGTTTTTGA
AGATTTGTTGAGAATTTGGCCTGAGTTATCTGAGGTGCCAGAAAAATATAGAGAAATGCTTACTATTAACGCTAGATATG
AGCCGTACTTACTCAGGCAAGAGCAGGATGTCAAACTACTTCGAAATAATGAGAAGGTCGTTATTCCTTCAAACTTTGAT
TTTGGTGCTATAAAGAGCCTTTCATCGGAAGTGATAGAAAAGCTTGAAGCTGTACGCCCGGAAACACTCGCTCAGGCTAA
GAGGATCTCAGGTGTTACACCTGCTGCAATTGTGTCAATTCTGATCCATTTGAGAAGATTAGGCGCTGAGGGATCCCAGC
CTTTTCCTAAGGATCTGGATCAGGCTCTTAGACAGCTAAATTAG

Upstream 100 bases:

>100_bases
ACTTGTACTACATAAAGGGGCTTTTGAGGGATTTAGGATTGCATATTAATACCACCTTTAAGCCTAAAGAGTTCATGTTG
CGTATTTTTCCTCGTAATTG

Downstream 100 bases:

>100_bases
TTTCAAAAAGAATCCACTTGTTAATGGAATGGCAATGTTTATCAGAGAGAGCGCCGACATTGATATGTGATATAGGATCG
AATTTCCTGCTTCAAATTTA

Product: tRNA uridine 5-carboxymethylaminomethyl modification protein GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 627; Mature: 627

Protein sequence:

>627_residues
MQYVVVIGGGHAGVEAAAASARLGVETLLVTNNVMNIGEMSCNPAIGGIGKGNVVKEVDAMGGVMALAIDRASIHSRMLN
RSKGAAVWGPRAQADRKLYKCAVLELLTKYEKLSILEDHITDLIIENDRLMGVVGEKTGTIKCSAAVLTTGTFLNGIIQT
GSERVEGGRFGEKASQCLGNTLRRHFKISRLRTGTPARLYKDSINYSALVEQPGDSPPIPFSYMNTEITVPQVSCYITHT
NGKTHEIIRNSLKFSAIRNGVSARGPRYCPSIEDKVVRFAEKDSHQIFLEPEGLDSELVYPNGISNSLPKEIQEEFIRSI
AGLEKASVARYAYTIEYDYIDPRELRSTLESKRVKNLYFAGQINGTTGYEEAAGQGIVAGSNAAGAGLIISRSEGYIGVM
IDDLITLGTNGEPYRLFTSRAEYRLNLRSDNADFRLTEKAYRVGLVDEERYAAYKKKYDTFHNYKSKLNELSTTPYELAK
IEGISIAQDGVRKSAWNLITQPLFVFEDLLRIWPELSEVPEKYREMLTINARYEPYLLRQEQDVKLLRNNEKVVIPSNFD
FGAIKSLSSEVIEKLEAVRPETLAQAKRISGVTPAAIVSILIHLRRLGAEGSQPFPKDLDQALRQLN

Sequences:

>Translated_627_residues
MQYVVVIGGGHAGVEAAAASARLGVETLLVTNNVMNIGEMSCNPAIGGIGKGNVVKEVDAMGGVMALAIDRASIHSRMLN
RSKGAAVWGPRAQADRKLYKCAVLELLTKYEKLSILEDHITDLIIENDRLMGVVGEKTGTIKCSAAVLTTGTFLNGIIQT
GSERVEGGRFGEKASQCLGNTLRRHFKISRLRTGTPARLYKDSINYSALVEQPGDSPPIPFSYMNTEITVPQVSCYITHT
NGKTHEIIRNSLKFSAIRNGVSARGPRYCPSIEDKVVRFAEKDSHQIFLEPEGLDSELVYPNGISNSLPKEIQEEFIRSI
AGLEKASVARYAYTIEYDYIDPRELRSTLESKRVKNLYFAGQINGTTGYEEAAGQGIVAGSNAAGAGLIISRSEGYIGVM
IDDLITLGTNGEPYRLFTSRAEYRLNLRSDNADFRLTEKAYRVGLVDEERYAAYKKKYDTFHNYKSKLNELSTTPYELAK
IEGISIAQDGVRKSAWNLITQPLFVFEDLLRIWPELSEVPEKYREMLTINARYEPYLLRQEQDVKLLRNNEKVVIPSNFD
FGAIKSLSSEVIEKLEAVRPETLAQAKRISGVTPAAIVSILIHLRRLGAEGSQPFPKDLDQALRQLN
>Mature_627_residues
MQYVVVIGGGHAGVEAAAASARLGVETLLVTNNVMNIGEMSCNPAIGGIGKGNVVKEVDAMGGVMALAIDRASIHSRMLN
RSKGAAVWGPRAQADRKLYKCAVLELLTKYEKLSILEDHITDLIIENDRLMGVVGEKTGTIKCSAAVLTTGTFLNGIIQT
GSERVEGGRFGEKASQCLGNTLRRHFKISRLRTGTPARLYKDSINYSALVEQPGDSPPIPFSYMNTEITVPQVSCYITHT
NGKTHEIIRNSLKFSAIRNGVSARGPRYCPSIEDKVVRFAEKDSHQIFLEPEGLDSELVYPNGISNSLPKEIQEEFIRSI
AGLEKASVARYAYTIEYDYIDPRELRSTLESKRVKNLYFAGQINGTTGYEEAAGQGIVAGSNAAGAGLIISRSEGYIGVM
IDDLITLGTNGEPYRLFTSRAEYRLNLRSDNADFRLTEKAYRVGLVDEERYAAYKKKYDTFHNYKSKLNELSTTPYELAK
IEGISIAQDGVRKSAWNLITQPLFVFEDLLRIWPELSEVPEKYREMLTINARYEPYLLRQEQDVKLLRNNEKVVIPSNFD
FGAIKSLSSEVIEKLEAVRPETLAQAKRISGVTPAAIVSILIHLRRLGAEGSQPFPKDLDQALRQLN

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=624, Percent_Identity=44.8717948717949, Blast_Score=487, Evalue=1e-137,
Organism=Homo sapiens, GI19882217, Length=649, Percent_Identity=43.1432973805855, Blast_Score=473, Evalue=1e-133,
Organism=Homo sapiens, GI183227703, Length=664, Percent_Identity=42.1686746987952, Blast_Score=468, Evalue=1e-132,
Organism=Escherichia coli, GI2367273, Length=616, Percent_Identity=49.1883116883117, Blast_Score=572, Evalue=1e-164,
Organism=Caenorhabditis elegans, GI17534255, Length=614, Percent_Identity=42.5081433224756, Blast_Score=474, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6321202, Length=636, Percent_Identity=42.2955974842767, Blast_Score=524, Evalue=1e-149,
Organism=Drosophila melanogaster, GI24658174, Length=627, Percent_Identity=47.2089314194577, Blast_Score=522, Evalue=1e-148,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_NEOSM (Q2GD63)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506583.1
- ProteinModelPortal:   Q2GD63
- SMR:   Q2GD63
- STRING:   Q2GD63
- GeneID:   3932258
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0707
- TIGR:   NSE_0707
- eggNOG:   COG0445
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- PhylomeDB:   Q2GD63
- ProtClustDB:   PRK05192
- BioCyc:   NSEN222891:NSE_0707-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 69432; Mature: 69432

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: BINDING 120-120 BINDING 175-175 BINDING 362-362

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYVVVIGGGHAGVEAAAASARLGVETLLVTNNVMNIGEMSCNPAIGGIGKGNVVKEVDA
CEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHH
MGGVMALAIDRASIHSRMLNRSKGAAVWGPRAQADRKLYKCAVLELLTKYEKLSILEDHI
HCCEEEEEEHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TDLIIENDRLMGVVGEKTGTIKCSAAVLTTGTFLNGIIQTGSERVEGGRFGEKASQCLGN
HHHEECCCCEEEEECCCCCEEEEEEHEEHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHH
TLRRHFKISRLRTGTPARLYKDSINYSALVEQPGDSPPIPFSYMNTEITVPQVSCYITHT
HHHHHHHHHHHCCCCCHHHHHHCCCCEEEEECCCCCCCCCEEECCCEEECCEEEEEEEEC
NGKTHEIIRNSLKFSAIRNGVSARGPRYCPSIEDKVVRFAEKDSHQIFLEPEGLDSELVY
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEC
PNGISNSLPKEIQEEFIRSIAGLEKASVARYAYTIEYDYIDPRELRSTLESKRVKNLYFA
CCCCCCCCCHHHHHHHHHHHHCCCHHHHEEEEEEEEECCCCHHHHHHHHHHHHHHHEEEE
GQINGTTGYEEAAGQGIVAGSNAAGAGLIISRSEGYIGVMIDDLITLGTNGEPYRLFTSR
EEECCCCCCHHHCCCCEEECCCCCCCCEEEECCCCEEEEEEHHHHEECCCCCCEEEEECC
AEYRLNLRSDNADFRLTEKAYRVGLVDEERYAAYKKKYDTFHNYKSKLNELSTTPYELAK
CEEEEEEECCCCCEEEEHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
IEGISIAQDGVRKSAWNLITQPLFVFEDLLRIWPELSEVPEKYREMLTINARYEPYLLRQ
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEECC
EQDVKLLRNNEKVVIPSNFDFGAIKSLSSEVIEKLEAVRPETLAQAKRISGVTPAAIVSI
CCCHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHH
LIHLRRLGAEGSQPFPKDLDQALRQLN
HHHHHHHCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MQYVVVIGGGHAGVEAAAASARLGVETLLVTNNVMNIGEMSCNPAIGGIGKGNVVKEVDA
CEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHH
MGGVMALAIDRASIHSRMLNRSKGAAVWGPRAQADRKLYKCAVLELLTKYEKLSILEDHI
HCCEEEEEEHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TDLIIENDRLMGVVGEKTGTIKCSAAVLTTGTFLNGIIQTGSERVEGGRFGEKASQCLGN
HHHEECCCCEEEEECCCCCEEEEEEHEEHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHH
TLRRHFKISRLRTGTPARLYKDSINYSALVEQPGDSPPIPFSYMNTEITVPQVSCYITHT
HHHHHHHHHHHCCCCCHHHHHHCCCCEEEEECCCCCCCCCEEECCCEEECCEEEEEEEEC
NGKTHEIIRNSLKFSAIRNGVSARGPRYCPSIEDKVVRFAEKDSHQIFLEPEGLDSELVY
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEC
PNGISNSLPKEIQEEFIRSIAGLEKASVARYAYTIEYDYIDPRELRSTLESKRVKNLYFA
CCCCCCCCCHHHHHHHHHHHHCCCHHHHEEEEEEEEECCCCHHHHHHHHHHHHHHHEEEE
GQINGTTGYEEAAGQGIVAGSNAAGAGLIISRSEGYIGVMIDDLITLGTNGEPYRLFTSR
EEECCCCCCHHHCCCCEEECCCCCCCCEEEECCCCEEEEEEHHHHEECCCCCCEEEEECC
AEYRLNLRSDNADFRLTEKAYRVGLVDEERYAAYKKKYDTFHNYKSKLNELSTTPYELAK
CEEEEEEECCCCCEEEEHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
IEGISIAQDGVRKSAWNLITQPLFVFEDLLRIWPELSEVPEKYREMLTINARYEPYLLRQ
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEECC
EQDVKLLRNNEKVVIPSNFDFGAIKSLSSEVIEKLEAVRPETLAQAKRISGVTPAAIVSI
CCCHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHH
LIHLRRLGAEGSQPFPKDLDQALRQLN
HHHHHHHCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA