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The map label for this gene is tig [H]

Identifier: 88608214

GI number: 88608214

Start: 668086

End: 669387

Strand: Direct

Name: tig [H]

Synonym: NSE_0751

Alternate gene names: 88608214

Gene position: 668086-669387 (Clockwise)

Preceding gene: 88608397

Following gene: 88608072

Centisome position: 77.77

GC content: 42.01

Gene sequence:

>1302_bases
ATGCTATTGAAGGAAGTTTATAGTCACGGCTTAAAAAGAAAATACGAGGTCGAGGTACCGGCATCCGATATAGATGCTGG
TTTCAAAGAGGCTATTGAAGACAGGATAAAGAAAAATGTCTATCGGGGTTTTAGGAAGGGTAAGACTCCCTTTGACGTCG
TTGCGAAACAGGAAGAAGGCCTGATAGATAGTGTTATCGTCGACCAAGTCTACAGACATTGGTACGACATTAAGCAAAAT
GAAAAGCTTGTCCTTTTCAATAACCAGGAAGAAATTACAGGTATAGATGCTACAGCATACAAAGAGAGTAAAGCTGCCGT
TAAGTTCACTGTTGAGTGCGAGCTTCTCCCACAGATCCCGAAGATAGACTTTTCCCAGGTAAGTGTCAAAACAAAATCAT
TTGAGGTGTCTGAGAAGGACATAGAAAAGTATAAACAAAAAGTGAAGGAGCAGCTCGTGGACTGGGTTGATCTTGCTCAG
TCTGATTCGATAGAGAGTGGAGATAGGGTAGTTGTTGATCTGCGCGGCAAAGTTGGCGATGAGGCATTTAGGGGTGGTCA
ACTTGATAACTTCGAATGTCAGGTCGGTGACGGCAAAGTAATTGCTCCTTTAAGTGACGGACTGATTGGCATGAAGGTTG
GTGAAGAACGCCTCATTCCAGTTCTGTTTCCGGAGGATTATCACGATATTGCAGTTGCCGGTAAAAATGCTGTTTTCCAT
ACTTTGGTAAAGAGTGGGAAGAGGTCGCAGAAAGTGTCAGATCCGGAAGCTGCACTACTCAAGCGGTTGTCCTGCAAAAC
AGATGACGAGACTAATGAAAAAGTAAAAAGCCTCCTCAACTTGGAATGCTCAAAAAGGATTAATGAACTTCTCAGGAGGC
AGATAGAAGCACAGTTGGAAAAATATGATTTTCAAGTTCCAGAATCACTTTTGGAAAGACAAGAACGCTATGTTAAGGTT
GATCTTCCAAGCATTTCTGCAGATGATCTTAAAAAAGAGGCTATGCGAAGAACCAGACTTTCGCTTATTCTGGCTGAGTT
CGCAGCAGAGAGAAATATATCGATTGATAAGACCGATGTTGCTCGTCATCTGCTAAATGTTTCCATGGCTGCTGGTCTAG
ATTCAAGCTATTTAGCAGAGATATATAGAAACAATAAGGACTTTGCAGGTTCGGTAACCCTTAAATTAATGGAAAGTAAG
ATATTTGCTGCATTACTGGAAACCGTGCAGAAGGAAGCTGAAGCTTCGAGTGTTGATGAAATACTCGGTTGTACAGAAGA
AAAAGTTGGGACACAGGTATAG

Upstream 100 bases:

>100_bases
GAATTGGTAGACGCGCTAGACTTAGGATCTAGTGGGCATCCCGTGGAGGTTCAAGTCCTCTCGTCCGTACCAGGATGCTT
TAGGGCTTAGGTCTATCGCT

Downstream 100 bases:

>100_bases
ACACAAATGGAAATGGATACTCTTGTCAGAGAAAAAATGAATCTTGTTCCGATGGTAGTTGATCAAACACCTCGGGGCGA
GAGAGCGTACGACATATTTT

Product: putative trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MLLKEVYSHGLKRKYEVEVPASDIDAGFKEAIEDRIKKNVYRGFRKGKTPFDVVAKQEEGLIDSVIVDQVYRHWYDIKQN
EKLVLFNNQEEITGIDATAYKESKAAVKFTVECELLPQIPKIDFSQVSVKTKSFEVSEKDIEKYKQKVKEQLVDWVDLAQ
SDSIESGDRVVVDLRGKVGDEAFRGGQLDNFECQVGDGKVIAPLSDGLIGMKVGEERLIPVLFPEDYHDIAVAGKNAVFH
TLVKSGKRSQKVSDPEAALLKRLSCKTDDETNEKVKSLLNLECSKRINELLRRQIEAQLEKYDFQVPESLLERQERYVKV
DLPSISADDLKKEAMRRTRLSLILAEFAAERNISIDKTDVARHLLNVSMAAGLDSSYLAEIYRNNKDFAGSVTLKLMESK
IFAALLETVQKEAEASSVDEILGCTEEKVGTQV

Sequences:

>Translated_433_residues
MLLKEVYSHGLKRKYEVEVPASDIDAGFKEAIEDRIKKNVYRGFRKGKTPFDVVAKQEEGLIDSVIVDQVYRHWYDIKQN
EKLVLFNNQEEITGIDATAYKESKAAVKFTVECELLPQIPKIDFSQVSVKTKSFEVSEKDIEKYKQKVKEQLVDWVDLAQ
SDSIESGDRVVVDLRGKVGDEAFRGGQLDNFECQVGDGKVIAPLSDGLIGMKVGEERLIPVLFPEDYHDIAVAGKNAVFH
TLVKSGKRSQKVSDPEAALLKRLSCKTDDETNEKVKSLLNLECSKRINELLRRQIEAQLEKYDFQVPESLLERQERYVKV
DLPSISADDLKKEAMRRTRLSLILAEFAAERNISIDKTDVARHLLNVSMAAGLDSSYLAEIYRNNKDFAGSVTLKLMESK
IFAALLETVQKEAEASSVDEILGCTEEKVGTQV
>Mature_433_residues
MLLKEVYSHGLKRKYEVEVPASDIDAGFKEAIEDRIKKNVYRGFRKGKTPFDVVAKQEEGLIDSVIVDQVYRHWYDIKQN
EKLVLFNNQEEITGIDATAYKESKAAVKFTVECELLPQIPKIDFSQVSVKTKSFEVSEKDIEKYKQKVKEQLVDWVDLAQ
SDSIESGDRVVVDLRGKVGDEAFRGGQLDNFECQVGDGKVIAPLSDGLIGMKVGEERLIPVLFPEDYHDIAVAGKNAVFH
TLVKSGKRSQKVSDPEAALLKRLSCKTDDETNEKVKSLLNLECSKRINELLRRQIEAQLEKYDFQVPESLLERQERYVKV
DLPSISADDLKKEAMRRTRLSLILAEFAAERNISIDKTDVARHLLNVSMAAGLDSSYLAEIYRNNKDFAGSVTLKLMESK
IFAALLETVQKEAEASSVDEILGCTEEKVGTQV

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=437, Percent_Identity=24.7139588100687, Blast_Score=114, Evalue=8e-27,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: NA

Molecular weight: Translated: 48862; Mature: 48862

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS50059 FKBP_PPIASE ; PS50198 PPIC_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLKEVYSHGLKRKYEVEVPASDIDAGFKEAIEDRIKKNVYRGFRKGKTPFDVVAKQEEG
CCHHHHHHHCCCEEEEEECCHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHCC
LIDSVIVDQVYRHWYDIKQNEKLVLFNNQEEITGIDATAYKESKAAVKFTVECELLPQIP
HHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHCCCCCEEEEEEEEEECCCCC
KIDFSQVSVKTKSFEVSEKDIEKYKQKVKEQLVDWVDLAQSDSIESGDRVVVDLRGKVGD
CCCCHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCC
EAFRGGQLDNFECQVGDGKVIAPLSDGLIGMKVGEERLIPVLFPEDYHDIAVAGKNAVFH
HHHCCCCCCCCEEEECCCEEEEECCCCEEEEEECCCCEEEEECCCCCCCCEEECCHHHHH
TLVKSGKRSQKVSDPEAALLKRLSCKTDDETNEKVKSLLNLECSKRINELLRRQIEAQLE
HHHHCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYDFQVPESLLERQERYVKVDLPSISADDLKKEAMRRTRLSLILAEFAAERNISIDKTDV
HHCCCCCHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
ARHLLNVSMAAGLDSSYLAEIYRNNKDFAGSVTLKLMESKIFAALLETVQKEAEASSVDE
HHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILGCTEEKVGTQV
HHCCCHHHCCCCC
>Mature Secondary Structure
MLLKEVYSHGLKRKYEVEVPASDIDAGFKEAIEDRIKKNVYRGFRKGKTPFDVVAKQEEG
CCHHHHHHHCCCEEEEEECCHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHCC
LIDSVIVDQVYRHWYDIKQNEKLVLFNNQEEITGIDATAYKESKAAVKFTVECELLPQIP
HHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHCCCCCEEEEEEEEEECCCCC
KIDFSQVSVKTKSFEVSEKDIEKYKQKVKEQLVDWVDLAQSDSIESGDRVVVDLRGKVGD
CCCCHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCC
EAFRGGQLDNFECQVGDGKVIAPLSDGLIGMKVGEERLIPVLFPEDYHDIAVAGKNAVFH
HHHCCCCCCCCEEEECCCEEEEECCCCEEEEEECCCCEEEEECCCCCCCCEEECCHHHHH
TLVKSGKRSQKVSDPEAALLKRLSCKTDDETNEKVKSLLNLECSKRINELLRRQIEAQLE
HHHHCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYDFQVPESLLERQERYVKVDLPSISADDLKKEAMRRTRLSLILAEFAAERNISIDKTDV
HHCCCCCHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
ARHLLNVSMAAGLDSSYLAEIYRNNKDFAGSVTLKLMESKIFAALLETVQKEAEASSVDE
HHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILGCTEEKVGTQV
HHCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA