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The map label for this gene is cysS

Identifier: 88608185

GI number: 88608185

Start: 271801

End: 273324

Strand: Direct

Name: cysS

Synonym: NSE_0333

Alternate gene names: 88608185

Gene position: 271801-273324 (Clockwise)

Preceding gene: 88608059

Following gene: 88608301

Centisome position: 31.64

GC content: 41.27

Gene sequence:

>1524_bases
ATGAAGTTGAGGGTTCATAATACACTTTCTGGCTTAAAGGAAGAATTTTTTCCTTTAAGCAGCGAAGTGGTGCGCATGTA
CGTGTGTGGACCAACTGTATACGATGTTCCGCATATAGGGAATATCAGGGCTTCTGTAGTGTATGACATTGTATATAGGG
TCCTACTTAAGCTTTTTCCTGAAGTTATTTATGTGCGTAACATTACTGATGTAGATGACAAGATTATAACAGCTGCGTCT
GTCCGAGGTGTCAAGTGTGATAAGGTTGCGCTTCATTACGAACATATTTTCCATGAGCATTTAGGCTTGCTTAATTGCCT
TTCGCCAACTGTTGAACCGAAAGCTACGCAAAACATAGGTAAAATGATTTCCATGATTCAAGCGCTTATAGATAATGGGA
ATGCTTACTCCGTGGGGGGAAATGTATATTTTGATGTAGGCTCATTTGCTGAATATGGGGTACTTTCCAAGAGAAAAAAA
GAGCAATTGGTCTACGGTGTGCGGGTAGAGAAGGATGTCGATAAAAAGCATCCAGGGGATTTCATATTGTGGAAAAGTGA
TGATCATGTATATTGGCCAAGTCCGTGGGGAAACGGGCGACCAGGGTGGCATATAGAGTGTTCTGCTATGACTTTAGCAA
CGTTAGGTGCAGATTTTGACATACATGGTGGTGGCGCAGACTTGAAGTTTCCCCATCACGAAAATGAAAGAGCTCAAAGT
ATGTGCGCTAATCCTGGTTCTCAATTTGCCCGCTACTGGGTTCATAATGGCTTTCTCACAGTAAATGGGGAAAAAATGAG
TAAGTCCCTAGGGAATGTTGTTAATGTTGATACGCTAGTTGAATCAGGTGTTACACCAAATGTGATCCGTTTTGTACTTA
TTAGCACTCACTACAGTAAGCCGTTGGACTGGAATGATGCCATGGTCGGAGAAGCGATTAACTCTTTAATAAAATTCAAG
CTTGCTCTTTTGGATAGCGGTGTTCTGCCTAAAAGTAAAAGCCATATAGGTAGTCTGAATATAGGAGTTTGTAGTGATAA
TGCTGAGGATGAGGAAGTCGACCCAAGAAGCTGCGCATCTGAGGTTATCGTTGCACGGAATAAGAGAAAAGCAAAAAACT
TCTCGAAAAAAATGAATGAGATTTTTAATAAGTTCGGGGGTTTAGGTGATGATTGTAGTATTTACACAAGGGAATTTTTT
GAGTGTATGGCAGATGATTTTAATACTCCTGGCGCTATTGCCGTTCTGCATAAACTTAGTGATGGGGTTCGTCTTGTTGG
TGCAGAAAAAGTGAATAATTTAGCACACCTTCTTTATAATTTATTGATCTTCTTAGGTATAGACTTAGGGGTGAGACAGT
CCAGTGTATCCGAAGATTTTATCCGTTCGCAATTGCAGAAGCGCGCTGATTGTAAGCGGCAAAAGGACTTTGTTGAGGCC
GATAGGATTCGCTTTGCGTTGGCTAAAATGGGAGTTCTAATCAGAGATCACAAGCATGCACCCACAGATTGGGTGTCTCT
TTAG

Upstream 100 bases:

>100_bases
TCAAACTAAGCAACCCTTGTCGCTTTTTGCTATGGTTTGTGTGCTTTTCCTCTTTCCAGGACTTTACGCGAATGTGCAGT
TGCATTTCTAGATCAGTGAA

Downstream 100 bases:

>100_bases
TACTGGCTTTGCCTTTGCAGCAGTATTGTACTTTACTTTAGAGTGCAGTATACTATATACACAATCCTGCTGTAAGTTTT
CAAGCATATATTGTTCGTAT

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MKLRVHNTLSGLKEEFFPLSSEVVRMYVCGPTVYDVPHIGNIRASVVYDIVYRVLLKLFPEVIYVRNITDVDDKIITAAS
VRGVKCDKVALHYEHIFHEHLGLLNCLSPTVEPKATQNIGKMISMIQALIDNGNAYSVGGNVYFDVGSFAEYGVLSKRKK
EQLVYGVRVEKDVDKKHPGDFILWKSDDHVYWPSPWGNGRPGWHIECSAMTLATLGADFDIHGGGADLKFPHHENERAQS
MCANPGSQFARYWVHNGFLTVNGEKMSKSLGNVVNVDTLVESGVTPNVIRFVLISTHYSKPLDWNDAMVGEAINSLIKFK
LALLDSGVLPKSKSHIGSLNIGVCSDNAEDEEVDPRSCASEVIVARNKRKAKNFSKKMNEIFNKFGGLGDDCSIYTREFF
ECMADDFNTPGAIAVLHKLSDGVRLVGAEKVNNLAHLLYNLLIFLGIDLGVRQSSVSEDFIRSQLQKRADCKRQKDFVEA
DRIRFALAKMGVLIRDHKHAPTDWVSL

Sequences:

>Translated_507_residues
MKLRVHNTLSGLKEEFFPLSSEVVRMYVCGPTVYDVPHIGNIRASVVYDIVYRVLLKLFPEVIYVRNITDVDDKIITAAS
VRGVKCDKVALHYEHIFHEHLGLLNCLSPTVEPKATQNIGKMISMIQALIDNGNAYSVGGNVYFDVGSFAEYGVLSKRKK
EQLVYGVRVEKDVDKKHPGDFILWKSDDHVYWPSPWGNGRPGWHIECSAMTLATLGADFDIHGGGADLKFPHHENERAQS
MCANPGSQFARYWVHNGFLTVNGEKMSKSLGNVVNVDTLVESGVTPNVIRFVLISTHYSKPLDWNDAMVGEAINSLIKFK
LALLDSGVLPKSKSHIGSLNIGVCSDNAEDEEVDPRSCASEVIVARNKRKAKNFSKKMNEIFNKFGGLGDDCSIYTREFF
ECMADDFNTPGAIAVLHKLSDGVRLVGAEKVNNLAHLLYNLLIFLGIDLGVRQSSVSEDFIRSQLQKRADCKRQKDFVEA
DRIRFALAKMGVLIRDHKHAPTDWVSL
>Mature_507_residues
MKLRVHNTLSGLKEEFFPLSSEVVRMYVCGPTVYDVPHIGNIRASVVYDIVYRVLLKLFPEVIYVRNITDVDDKIITAAS
VRGVKCDKVALHYEHIFHEHLGLLNCLSPTVEPKATQNIGKMISMIQALIDNGNAYSVGGNVYFDVGSFAEYGVLSKRKK
EQLVYGVRVEKDVDKKHPGDFILWKSDDHVYWPSPWGNGRPGWHIECSAMTLATLGADFDIHGGGADLKFPHHENERAQS
MCANPGSQFARYWVHNGFLTVNGEKMSKSLGNVVNVDTLVESGVTPNVIRFVLISTHYSKPLDWNDAMVGEAINSLIKFK
LALLDSGVLPKSKSHIGSLNIGVCSDNAEDEEVDPRSCASEVIVARNKRKAKNFSKKMNEIFNKFGGLGDDCSIYTREFF
ECMADDFNTPGAIAVLHKLSDGVRLVGAEKVNNLAHLLYNLLIFLGIDLGVRQSSVSEDFIRSQLQKRADCKRQKDFVEA
DRIRFALAKMGVLIRDHKHAPTDWVSL

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=315, Percent_Identity=42.2222222222222, Blast_Score=240, Evalue=3e-63,
Organism=Homo sapiens, GI62240992, Length=242, Percent_Identity=36.7768595041322, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI21269877, Length=242, Percent_Identity=36.7768595041322, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI10835051, Length=242, Percent_Identity=36.7768595041322, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI303304987, Length=242, Percent_Identity=36.7768595041322, Blast_Score=165, Evalue=1e-40,
Organism=Escherichia coli, GI1786737, Length=515, Percent_Identity=37.6699029126214, Blast_Score=292, Evalue=4e-80,
Organism=Caenorhabditis elegans, GI17509795, Length=249, Percent_Identity=35.3413654618474, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17509797, Length=249, Percent_Identity=35.3413654618474, Blast_Score=159, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6324082, Length=238, Percent_Identity=36.5546218487395, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI19922218, Length=526, Percent_Identity=32.319391634981, Blast_Score=215, Evalue=6e-56,
Organism=Drosophila melanogaster, GI19922364, Length=230, Percent_Identity=36.9565217391304, Blast_Score=165, Evalue=8e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_NEOSM (Q2GE74)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506222.1
- ProteinModelPortal:   Q2GE74
- SMR:   Q2GE74
- STRING:   Q2GE74
- GeneID:   3931752
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0333
- TIGR:   NSE_0333
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   HIGNIRA
- ProtClustDB:   CLSK2527765
- BioCyc:   NSEN222891:NSE_0333-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 56617; Mature: 56617

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 268-268

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRVHNTLSGLKEEFFPLSSEVVRMYVCGPTVYDVPHIGNIRASVVYDIVYRVLLKLFP
CEEEEHHHHHHHHHHHCCCCHHHEEEEEECCCEECCCCCCCHHHHHHHHHHHHHHHHHHH
EVIYVRNITDVDDKIITAASVRGVKCDKVALHYEHIFHEHLGLLNCLSPTVEPKATQNIG
HHEECCCCCCCCHHHEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
KMISMIQALIDNGNAYSVGGNVYFDVGSFAEYGVLSKRKKEQLVYGVRVEKDVDKKHPGD
HHHHHHHHHHCCCCEEEECCEEEEECCCCHHHHHHHHHHHHHHEEEEEECCCCCCCCCCC
FILWKSDDHVYWPSPWGNGRPGWHIECSAMTLATLGADFDIHGGGADLKFPHHENERAQS
EEEEECCCEEECCCCCCCCCCCEEEEEHHEEEEHHCCCEEECCCCCCCCCCCCCCHHHHH
MCANPGSQFARYWVHNGFLTVNGEKMSKSLGNVVNVDTLVESGVTPNVIRFVLISTHYSK
HHCCCHHHHHHHHHHCCEEEECHHHHHHHHCCCEEHHHHHHCCCCHHHHHHHHEECCCCC
PLDWNDAMVGEAINSLIKFKLALLDSGVLPKSKSHIGSLNIGVCSDNAEDEEVDPRSCAS
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHH
EVIVARNKRKAKNFSKKMNEIFNKFGGLGDDCSIYTREFFECMADDFNTPGAIAVLHKLS
HHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
DGVRLVGAEKVNNLAHLLYNLLIFLGIDLGVRQSSVSEDFIRSQLQKRADCKRQKDFVEA
CCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRIRFALAKMGVLIRDHKHAPTDWVSL
HHHHHHHHHHHHHEECCCCCCCHHCCC
>Mature Secondary Structure
MKLRVHNTLSGLKEEFFPLSSEVVRMYVCGPTVYDVPHIGNIRASVVYDIVYRVLLKLFP
CEEEEHHHHHHHHHHHCCCCHHHEEEEEECCCEECCCCCCCHHHHHHHHHHHHHHHHHHH
EVIYVRNITDVDDKIITAASVRGVKCDKVALHYEHIFHEHLGLLNCLSPTVEPKATQNIG
HHEECCCCCCCCHHHEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
KMISMIQALIDNGNAYSVGGNVYFDVGSFAEYGVLSKRKKEQLVYGVRVEKDVDKKHPGD
HHHHHHHHHHCCCCEEEECCEEEEECCCCHHHHHHHHHHHHHHEEEEEECCCCCCCCCCC
FILWKSDDHVYWPSPWGNGRPGWHIECSAMTLATLGADFDIHGGGADLKFPHHENERAQS
EEEEECCCEEECCCCCCCCCCCEEEEEHHEEEEHHCCCEEECCCCCCCCCCCCCCHHHHH
MCANPGSQFARYWVHNGFLTVNGEKMSKSLGNVVNVDTLVESGVTPNVIRFVLISTHYSK
HHCCCHHHHHHHHHHCCEEEECHHHHHHHHCCCEEHHHHHHCCCCHHHHHHHHEECCCCC
PLDWNDAMVGEAINSLIKFKLALLDSGVLPKSKSHIGSLNIGVCSDNAEDEEVDPRSCAS
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHH
EVIVARNKRKAKNFSKKMNEIFNKFGGLGDDCSIYTREFFECMADDFNTPGAIAVLHKLS
HHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
DGVRLVGAEKVNNLAHLLYNLLIFLGIDLGVRQSSVSEDFIRSQLQKRADCKRQKDFVEA
CCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRIRFALAKMGVLIRDHKHAPTDWVSL
HHHHHHHHHHHHHEECCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA