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The map label for this gene is pdhB [H]

Identifier: 88608183

GI number: 88608183

Start: 665846

End: 666844

Strand: Direct

Name: pdhB [H]

Synonym: NSE_0746

Alternate gene names: 88608183

Gene position: 665846-666844 (Clockwise)

Preceding gene: 88608039

Following gene: 88608602

Centisome position: 77.51

GC content: 44.74

Gene sequence:

>999_bases
GTGACAGAAATCACAGTAAGAGAAGCAATAAGAAATGCAATGGCCGAGGAAATGCGCCGTGACTCCGACGTTTTCATTAT
CGGTGAAGAGGTGGGTAAGTATCAAGGAGCATATAAAGTAACACAAGGGCTCCTCGAAGAATTCGGCGAAAAAAGGGTCG
TGGATACACCCATAAGCGAACATGCTTTTGCAGGTATAGCCACTGGAGCGGCTTTTGTAGGTCTGAGACCAATAGTCGAG
TTTATGTCATTTAATTTCTCACTACAAGCAATGGACCAGATTCTTAACTCTGCCGCAAAAACACATTACATGTCTGGTGG
ACGTCTGAGCTGTCCTATCGTCTTCAGGGGACCTAATGGGGCTGCTGTTCAAGTAGGCGCGCAACATTCACAATGTTTTG
CTGCGTGGTATTCTCACGTACCCGGTTTAAAGGTAGTAGCTCCATACTTTGCGTCCGATTGTCGTGGATTGCTAAAGTCA
GCAGTAAGAGATAACAACCCTGTCATCTTCTTAGAAAACGAAAGGACATACGGGCTCGTACATACTCTTACTGCAGAGCA
AGAAGCAGAGGATTACTTGGTTCCAATTGGGGAGGCAAACGTTCTCAGAAATGGTACAGATGTCACGATAGTGACATTTT
CGATTTGTGTAGAACTAGCACTTGAAGCAGCAGAAGCGCTAGAAAGCGAACATAACATAAGTGTTGAGGTAATAGATTTG
CGTACACTTAGACCGCTAGATTTCCAAACGATCATTAGATCCCTTGAAAAGACGAATAAATTAGTCACACTAGAACAGGG
TTTTCCCGTTCTGAGTTTCGGATCTGAAGTATCAGCAAGAATCATGGAGGAAGGCTTCGATTTACTAGATGCGCCTGTGG
TCAGAATTTCTGGCAGAGACGTTCCAATGCCATACTCTAGTGCACTAGAAAAGCTGGCTCTTCCTCAACTACCTGAGGTG
ATAGAGGTCGTAAAGAAAGTAGCCACCAGCGGAATCTAG

Upstream 100 bases:

>100_bases
AAAACTCACACTACAATGGGTTCTAATTTAAGAAAGAAACTAGCAAGGTAACGCTAAGTGGACTATCATACTGTTCTGTT
AGCCGATCTTTCAAAGCACA

Downstream 100 bases:

>100_bases
TTGCTTACTCCGCTCATAACTCCACGCTGAGATGCGTCAGGACTGGTAGCATCTGCCCTTAAATTTGAACTGTGAGTTGT
GGCTAAAATCGCTTACCAGA

Product: pyruvate dehydrogenase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MTEITVREAIRNAMAEEMRRDSDVFIIGEEVGKYQGAYKVTQGLLEEFGEKRVVDTPISEHAFAGIATGAAFVGLRPIVE
FMSFNFSLQAMDQILNSAAKTHYMSGGRLSCPIVFRGPNGAAVQVGAQHSQCFAAWYSHVPGLKVVAPYFASDCRGLLKS
AVRDNNPVIFLENERTYGLVHTLTAEQEAEDYLVPIGEANVLRNGTDVTIVTFSICVELALEAAEALESEHNISVEVIDL
RTLRPLDFQTIIRSLEKTNKLVTLEQGFPVLSFGSEVSARIMEEGFDLLDAPVVRISGRDVPMPYSSALEKLALPQLPEV
IEVVKKVATSGI

Sequences:

>Translated_332_residues
MTEITVREAIRNAMAEEMRRDSDVFIIGEEVGKYQGAYKVTQGLLEEFGEKRVVDTPISEHAFAGIATGAAFVGLRPIVE
FMSFNFSLQAMDQILNSAAKTHYMSGGRLSCPIVFRGPNGAAVQVGAQHSQCFAAWYSHVPGLKVVAPYFASDCRGLLKS
AVRDNNPVIFLENERTYGLVHTLTAEQEAEDYLVPIGEANVLRNGTDVTIVTFSICVELALEAAEALESEHNISVEVIDL
RTLRPLDFQTIIRSLEKTNKLVTLEQGFPVLSFGSEVSARIMEEGFDLLDAPVVRISGRDVPMPYSSALEKLALPQLPEV
IEVVKKVATSGI
>Mature_331_residues
TEITVREAIRNAMAEEMRRDSDVFIIGEEVGKYQGAYKVTQGLLEEFGEKRVVDTPISEHAFAGIATGAAFVGLRPIVEF
MSFNFSLQAMDQILNSAAKTHYMSGGRLSCPIVFRGPNGAAVQVGAQHSQCFAAWYSHVPGLKVVAPYFASDCRGLLKSA
VRDNNPVIFLENERTYGLVHTLTAEQEAEDYLVPIGEANVLRNGTDVTIVTFSICVELALEAAEALESEHNISVEVIDLR
TLRPLDFQTIIRSLEKTNKLVTLEQGFPVLSFGSEVSARIMEEGFDLLDAPVVRISGRDVPMPYSSALEKLALPQLPEVI
EVVKKVATSGI

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=325, Percent_Identity=56.6153846153846, Blast_Score=396, Evalue=1e-110,
Organism=Homo sapiens, GI291084858, Length=325, Percent_Identity=53.2307692307692, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI4557353, Length=326, Percent_Identity=32.8220858895706, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI34101272, Length=326, Percent_Identity=32.8220858895706, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17538422, Length=326, Percent_Identity=58.2822085889571, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17506935, Length=326, Percent_Identity=33.4355828220859, Blast_Score=178, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6319698, Length=325, Percent_Identity=57.2307692307692, Blast_Score=404, Evalue=1e-114,
Organism=Drosophila melanogaster, GI21358145, Length=326, Percent_Identity=56.7484662576687, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24650940, Length=326, Percent_Identity=56.7484662576687, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI160714832, Length=321, Percent_Identity=34.8909657320872, Blast_Score=196, Evalue=3e-50,
Organism=Drosophila melanogaster, GI160714828, Length=321, Percent_Identity=34.8909657320872, Blast_Score=195, Evalue=3e-50,
Organism=Drosophila melanogaster, GI24650943, Length=98, Percent_Identity=57.1428571428571, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24650945, Length=98, Percent_Identity=57.1428571428571, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 36354; Mature: 36223

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEITVREAIRNAMAEEMRRDSDVFIIGEEVGKYQGAYKVTQGLLEEFGEKRVVDTPISE
CCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCCHHHHHHHHHHHHCCCCEECCCCHH
HAFAGIATGAAFVGLRPIVEFMSFNFSLQAMDQILNSAAKTHYMSGGRLSCPIVFRGPNG
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEECEEEEECCCC
AAVQVGAQHSQCFAAWYSHVPGLKVVAPYFASDCRGLLKSAVRDNNPVIFLENERTYGLV
CEEEECCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEE
HTLTAEQEAEDYLVPIGEANVLRNGTDVTIVTFSICVELALEAAEALESEHNISVEVIDL
EEECCCCCCCCCEEECCCCHHCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
RTLRPLDFQTIIRSLEKTNKLVTLEQGFPVLSFGSEVSARIMEEGFDLLDAPVVRISGRD
CCCCCCCHHHHHHHHHHCCCEEEEECCCCEEECCHHHHHHHHHHCCHHHCCCEEEECCCC
VPMPYSSALEKLALPQLPEVIEVVKKVATSGI
CCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TEITVREAIRNAMAEEMRRDSDVFIIGEEVGKYQGAYKVTQGLLEEFGEKRVVDTPISE
CCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCCHHHHHHHHHHHHCCCCEECCCCHH
HAFAGIATGAAFVGLRPIVEFMSFNFSLQAMDQILNSAAKTHYMSGGRLSCPIVFRGPNG
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEECEEEEECCCC
AAVQVGAQHSQCFAAWYSHVPGLKVVAPYFASDCRGLLKSAVRDNNPVIFLENERTYGLV
CEEEECCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEE
HTLTAEQEAEDYLVPIGEANVLRNGTDVTIVTFSICVELALEAAEALESEHNISVEVIDL
EEECCCCCCCCCEEECCCCHHCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
RTLRPLDFQTIIRSLEKTNKLVTLEQGFPVLSFGSEVSARIMEEGFDLLDAPVVRISGRD
CCCCCCCHHHHHHHHHHCCCEEEEECCCCEEECCHHHHHHHHHHCCHHHCCCEEEECCCC
VPMPYSSALEKLALPQLPEVIEVVKKVATSGI
CCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]