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The map label for this gene is pyrC

Identifier: 88608169

GI number: 88608169

Start: 123195

End: 124511

Strand: Direct

Name: pyrC

Synonym: NSE_0154

Alternate gene names: 88608169

Gene position: 123195-124511 (Clockwise)

Preceding gene: 88607987

Following gene: 88608728

Centisome position: 14.34

GC content: 43.2

Gene sequence:

>1317_bases
ATGAAAAAATGGAACCTACCAACCGGTAGTGACAGAAAGGTTGCCTTCGTTGATGCTAGAATCGTTGATCCAGAAAGCGG
ATTAGAGATTCTTGGGGATTTGGTTGTTCATGGGAGGCGGATTGGTGATTTTGGGGAATCCCTGCTTAGTGATGTTGAAC
TTAAATCTTTCGATGAAGTTGTGAATTGTGATGGGCATATTCTCATGCCTGGCATTATAGACATACACGTTCATTTAAGG
GATCCAGGACAATTACAAAACGAAGATATACATTCTGGTACCAAATCTGCAGCTGCTGGTGGTGTGACTACTGTTGTTTG
TCAACCTAATACTGATCCTCCGATTGATACAGTTGAAACTCTGGCATACATACGAGATAAGGCGAAAAGAGTTGGTTTTG
TAAACGTCCTATGTTACGCGTCGATTACTGGGAGGGGGGGAGATCTTACTGATATGTTTGCCTTGCACAAAGCTGGCGCA
GTTGGTTTCACGGATGATGGTCTTCCAGTTATGAACTCACTTTTTATGAGACAAGCTTTCATGAACGCTGCACTTCTGAA
GGTACCGGTAGCACAGCACGCAGAGGATCTCGATCTTTCTAATGGTGGCTGCATAAACGAGGGGGCGATATCTCATAAGT
TAAATGTTCCGGGAATTTCACATCTTGCTGAGTCTGTCATGGTTGCCAGAGATGTATTACTTTTAGAACAACTTGGTGCA
CATTACCACGTGTTACATGTCTCAACGAAGAAAACAGTTGAAATAGTCAGAGCAGCAAAAGATAAGGGAATGAGAGTGAC
CTGTGAAGTAACACCACACCACCTTCTTTTAACCGAAGAGGCTGTAGATGGATATAACACCGCTGCTAAAATGAACCCTC
CGCTGCGAACAGAAGAAGATCGCCTCTGCATGATTGAGGCGCTTAAAGATGGCACTATTGATGCTATAGCTTCAGATCAT
GCACCACATAACGAGGTTTATAAGGAGCTTCCGCTAAAAGAAGCTGCGTTTGGAGTGGTTGGATTAGAGACAATACTACC
ACTGTCATTAGAGTTATATCACTCTGGGGCTCTGGAGCTGCATTCATTGCTTGCAAAACTGACATGCAATCCTGCAAAGA
TTATTGGTTCTGAGGCTGGAAGAATAAAGGTGGGTGCTCCTGCGGACCTTGTTCTTCTGGATCTTAATTCAGAGGTAAGA
ATAGAGACAAGACAGTTTGTAAGTAAATCAAAGAATTCGCCTTTTGGAGGACGTAAGACCAGAGGCAAAGTTTTACGTAC
TATTGTTGGTGGAGATACAGTGTATATGGATGTGTAA

Upstream 100 bases:

>100_bases
TGGTGTCACGCATGAAAGCAATAACAATGTTTGAGAATTGTGAGTTTACTAGATTAGATGTGTTTGCTATTCTGACAGCA
AGTTCTCGTCGCGTTTTTGG

Downstream 100 bases:

>100_bases
GAAAGCTCCCACTAATATCTCTTTCTTTTGAGGTTTCAGCACCAAATTGCTTGTTTACACCGACTTTTTAGGTATTGTCC
TGTGTAGATGCGGTGCATAT

Product: dihydroorotase, multifunctional complex type

Products: NA

Alternate protein names: DHOase [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MKKWNLPTGSDRKVAFVDARIVDPESGLEILGDLVVHGRRIGDFGESLLSDVELKSFDEVVNCDGHILMPGIIDIHVHLR
DPGQLQNEDIHSGTKSAAAGGVTTVVCQPNTDPPIDTVETLAYIRDKAKRVGFVNVLCYASITGRGGDLTDMFALHKAGA
VGFTDDGLPVMNSLFMRQAFMNAALLKVPVAQHAEDLDLSNGGCINEGAISHKLNVPGISHLAESVMVARDVLLLEQLGA
HYHVLHVSTKKTVEIVRAAKDKGMRVTCEVTPHHLLLTEEAVDGYNTAAKMNPPLRTEEDRLCMIEALKDGTIDAIASDH
APHNEVYKELPLKEAAFGVVGLETILPLSLELYHSGALELHSLLAKLTCNPAKIIGSEAGRIKVGAPADLVLLDLNSEVR
IETRQFVSKSKNSPFGGRKTRGKVLRTIVGGDTVYMDV

Sequences:

>Translated_438_residues
MKKWNLPTGSDRKVAFVDARIVDPESGLEILGDLVVHGRRIGDFGESLLSDVELKSFDEVVNCDGHILMPGIIDIHVHLR
DPGQLQNEDIHSGTKSAAAGGVTTVVCQPNTDPPIDTVETLAYIRDKAKRVGFVNVLCYASITGRGGDLTDMFALHKAGA
VGFTDDGLPVMNSLFMRQAFMNAALLKVPVAQHAEDLDLSNGGCINEGAISHKLNVPGISHLAESVMVARDVLLLEQLGA
HYHVLHVSTKKTVEIVRAAKDKGMRVTCEVTPHHLLLTEEAVDGYNTAAKMNPPLRTEEDRLCMIEALKDGTIDAIASDH
APHNEVYKELPLKEAAFGVVGLETILPLSLELYHSGALELHSLLAKLTCNPAKIIGSEAGRIKVGAPADLVLLDLNSEVR
IETRQFVSKSKNSPFGGRKTRGKVLRTIVGGDTVYMDV
>Mature_438_residues
MKKWNLPTGSDRKVAFVDARIVDPESGLEILGDLVVHGRRIGDFGESLLSDVELKSFDEVVNCDGHILMPGIIDIHVHLR
DPGQLQNEDIHSGTKSAAAGGVTTVVCQPNTDPPIDTVETLAYIRDKAKRVGFVNVLCYASITGRGGDLTDMFALHKAGA
VGFTDDGLPVMNSLFMRQAFMNAALLKVPVAQHAEDLDLSNGGCINEGAISHKLNVPGISHLAESVMVARDVLLLEQLGA
HYHVLHVSTKKTVEIVRAAKDKGMRVTCEVTPHHLLLTEEAVDGYNTAAKMNPPLRTEEDRLCMIEALKDGTIDAIASDH
APHNEVYKELPLKEAAFGVVGLETILPLSLELYHSGALELHSLLAKLTCNPAKIIGSEAGRIKVGAPADLVLLDLNSEVR
IETRQFVSKSKNSPFGGRKTRGKVLRTIVGGDTVYMDV

Specific function: Involved In The Anaerobic Utilization Of Allantoin. [C]

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=376, Percent_Identity=32.9787234042553, Blast_Score=155, Evalue=6e-38,
Organism=Escherichia coli, GI1786722, Length=420, Percent_Identity=27.3809523809524, Blast_Score=148, Evalue=6e-37,
Organism=Escherichia coli, GI87082175, Length=410, Percent_Identity=23.6585365853659, Blast_Score=86, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI193204318, Length=374, Percent_Identity=30.2139037433155, Blast_Score=154, Evalue=9e-38,
Organism=Saccharomyces cerevisiae, GI6322218, Length=405, Percent_Identity=28.3950617283951, Blast_Score=142, Evalue=8e-35,
Organism=Drosophila melanogaster, GI24642586, Length=374, Percent_Identity=33.6898395721925, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI18859883, Length=416, Percent_Identity=27.4038461538462, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR004722
- InterPro:   IPR002195
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.2.3 [H]

Molecular weight: Translated: 47314; Mature: 47314

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00482 DIHYDROOROTASE_1 ; PS00483 DIHYDROOROTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWNLPTGSDRKVAFVDARIVDPESGLEILGDLVVHGRRIGDFGESLLSDVELKSFDEV
CCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHH
VNCDGHILMPGIIDIHVHLRDPGQLQNEDIHSGTKSAAAGGVTTVVCQPNTDPPIDTVET
HCCCCCEEECCEEEEEEEECCCCCCCCCCCCCCCCHHCCCCEEEEEECCCCCCCHHHHHH
LAYIRDKAKRVGFVNVLCYASITGRGGDLTDMFALHKAGAVGFTDDGLPVMNSLFMRQAF
HHHHHHHHHHCCEEEEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
MNAALLKVPVAQHAEDLDLSNGGCINEGAISHKLNVPGISHLAESVMVARDVLLLEQLGA
HHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCC
HYHVLHVSTKKTVEIVRAAKDKGMRVTCEVTPHHLLLTEEAVDGYNTAAKMNPPLRTEED
EEEEEEECCHHHHHHHHHHHCCCCEEEEEECCCEEEEEHHHCCCCCCCCCCCCCCCCCCC
RLCMIEALKDGTIDAIASDHAPHNEVYKELPLKEAAFGVVGLETILPLSLELYHSGALEL
CEEEEEHHCCCCEEEHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEHHCCHHHH
HSLLAKLTCNPAKIIGSEAGRIKVGAPADLVLLDLNSEVRIETRQFVSKSKNSPFGGRKT
HHHHHHHCCCHHHHHCCCCCEEEECCCCCEEEEECCCCEEEHHHHHHHHCCCCCCCCCCH
RGKVLRTIVGGDTVYMDV
HHHHHHHEECCCEEEEEC
>Mature Secondary Structure
MKKWNLPTGSDRKVAFVDARIVDPESGLEILGDLVVHGRRIGDFGESLLSDVELKSFDEV
CCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHH
VNCDGHILMPGIIDIHVHLRDPGQLQNEDIHSGTKSAAAGGVTTVVCQPNTDPPIDTVET
HCCCCCEEECCEEEEEEEECCCCCCCCCCCCCCCCHHCCCCEEEEEECCCCCCCHHHHHH
LAYIRDKAKRVGFVNVLCYASITGRGGDLTDMFALHKAGAVGFTDDGLPVMNSLFMRQAF
HHHHHHHHHHCCEEEEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
MNAALLKVPVAQHAEDLDLSNGGCINEGAISHKLNVPGISHLAESVMVARDVLLLEQLGA
HHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCC
HYHVLHVSTKKTVEIVRAAKDKGMRVTCEVTPHHLLLTEEAVDGYNTAAKMNPPLRTEED
EEEEEEECCHHHHHHHHHHHCCCCEEEEEECCCEEEEEHHHCCCCCCCCCCCCCCCCCCC
RLCMIEALKDGTIDAIASDHAPHNEVYKELPLKEAAFGVVGLETILPLSLELYHSGALEL
CEEEEEHHCCCCEEEHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEHHCCHHHH
HSLLAKLTCNPAKIIGSEAGRIKVGAPADLVLLDLNSEVRIETRQFVSKSKNSPFGGRKT
HHHHHHHCCCHHHHHCCCCCEEEECCCCCEEEEECCCCEEEHHHHHHHHCCCCCCCCCCH
RGKVLRTIVGGDTVYMDV
HHHHHHHEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA