Click here to switch to the map view.

The map label for this gene is addA [H]

Identifier: 88608144

GI number: 88608144

Start: 383147

End: 385870

Strand: Reverse

Name: addA [H]

Synonym: NSE_0467

Alternate gene names: 88608144

Gene position: 385870-383147 (Counterclockwise)

Preceding gene: 88608438

Following gene: 88608773

Centisome position: 44.92

GC content: 36.93

Gene sequence:

>2724_bases
ATGATAGAAAAAGTTACTGATCCTAATGTCTCAGTCTGGATTAATTCATCCGCCGGTTGCGGCAAAACCACCTTGCTTGT
AAGAAGAGCCATATCATTACTGGTTAACAAAGAGAAGAATATACTGTGCATAACTTTTACAAAGGTAGCAACAGCAGAAA
TGCACAACCGCATTTTCGCTATATTAGGAAAGCTCTCCGCAATGAATGATACAGATATGGACGAGTATTTGCTTTCCACG
ATTAATAGAACTGTGAAAGATCGGGATTATGTCCGGAAACTTGTGTACACAGCCGACGCATTTATCCAAATACAGACATT
ACACTCCTTCTGCTGGCAATCCATTAAAATGTGTGACCGACGACATCTTTCAAAAGAAATATATGAGGAAAACCAACACA
TGTTCTGCCAATTACTGGCACGGTTTATTTGGGAAAGATGTCAACTTTCCAAGAAAACCGCAGAAAAGACAACCGAAAGA
AAATTGCATGAAACGCTAGCATCTTTGCTAAGTAACTTTCACCTAGACTACAGAAAAATAATCGAGAAACTTAAGAATGA
TCCACTAGAACAATTGGCAACGGAGAGTATAGATATATTATCGGCATTCACTCAATTTTTAGAAATTAATCAAAATATCT
CGAAACACTATATTTCCCATAATGGAATCATAGAGACTGCGATAGAGATGGAAATATGGAATGTTTATGAAATTGCTGCG
AAAATAGACCATATCCTGCTCGATGAAGCGCAGGACAATAGCATAAAACAGTGGTATATCATATCTAATCTCTGTAGGGA
TTTTCTCATAGATGAAACACATAATAAGAGCATCTTTGTAGTTGGTGACTACAAACAGTCCATTTATGGGTTTCAGGGGG
CGAGTCCCGAGATGTATCTTGCGTTTTACGACATACTTAAACAAAGGGATCCCCAAAATAAGCTTGTTCAGGTTAAGACA
GCAAAGTCCTATAGATCATCTACAGCTATTCTACAATTTGTTGACAGCGTCTTTGAAAACATCCAACTTTGTAAAACATC
CAGTGAGAAGATTCGCCATACCACAAGTAGAACTGAAGCGCCGGGCTATATAGAAGTAATACCATTGCTACAATATGGAG
AAGTTGATGCAGAACTTCAACTGGCAATTTCTATAAGGGAAACCATACAAAGTTGGCTTAAGATAGGTAGAACACTTAAA
GCAAAAAACAGACCTGTAAAAGGAAGTGACATATTGATCCTTGTTGCACACAGAACCAATTTGGTTGACCATATTTACAC
GCAACTTACTGCCATTAATATCCCGGTGAATTTTACAGGCAAATTCACAGTAAAAAACAATCCCTTGTTCGAGCTTCTCA
TAAATCTAGGAAAGTTTCTCATCTATAAACACGATGATGAAGCTCTGATCACACTTTTGAAATCTCCTATATTTGCCTGG
AGCCACAGTGCTCTACTAAAACTTAAAGCTGAGAAAAAAAAGGAATGGTTGTTCGAGCAGATTTGTGCCACATACGCAGG
AAAAGTACTTAAGAGGTGGCTTGAATTCTATGGCACGACTTTCGACATATATTCACAGGTTCTTGACGCAAGGACTGTCA
CCACATTAGCCAAATACTATGGAAGTGAAACCCCCTATTATATTGACATCTTTTTAGACAACACGCTCGAACACGAATGT
ATACATGATTTTATTTACATCTTTTCTGGCGAAGACGCGACTTTAAAAATCAAAAGCAAGAACGAAAACGGTGTTACTAT
CTCAACCGTTCATGGAAGTAAAGGTACGCAATCACCTATTGTGTTTATAGTGGATAGTAATTACGTACCACAAAATCGTG
ATATTTTTTTCTTTACTGAAGACGGAACACCACTCCTCTCATCTGAAACCCATGTTAGAGTCGTACAAGAAATGAGGGAT
GTAAAAAAGGAATCGATCCGATATGAACATCTGAGACTCCTCTACGTTGCCTTAACACGTGCGGAAGATGAATTGTACAT
TATGGGAATTGGCAAACAAGCCAAAAAGAATTCTTGGTATGAAGCATGCAGCAACGGAATCGTAAAAATAGGCTCACAAA
ATCAAGATGGAGCGTATTACTACACAAATGATCTCCTGTATCCTATAAAAATTAAACAAAAGGTAGATAAATCAGACGAA
AACCCCAGTCTACATAGAGAAAGAGATAGCAACCGTCGCATGAAAAATACGCAAGAGCTACAAAACAAGGAAAGTGATTT
AGCAAACTCATATGAATTTCCTTACAAAAGAGATGAAACATTTGATAATGGCATCATAGATGATGATGTAACAAAAGTTT
CTGAGAAGACCTTTCAGATCATCCGTGGCAAATTAATACACAAGATTCTCGAATACATTTCTAAAGTTGAAAAAGCTGAA
AAATGGATGAATTTATTCCTGGAAGAGTATTCTCCAGAATTATCGAACGAGCAAAAAAATGAAATCAAAAGGACGGTTTT
GCTTTTTCTTGAAAAGAATCATAGAGCGGACGGTAAAAACGAGGTAGAAGTCTATTTTGATGGTAAGCTACTCAGAATGG
ATAATGTGAAATTCGAAAAGGATACAATAACGATTAGAGATTATAAAACTGGAAAAGATAAAAGCGTCGATCTTGCAATA
GAAAAGCAAATGCATCGATATAAAGAAGCTATGCAAGCAATCTATCCAGAAAAGAATGTAATAACTGAAATAATTTGGCT
TTAA

Upstream 100 bases:

>100_bases
ATCACATCAAGATTCTTTTAGCATCGCTCATAACATATTGCATATCCTATGCTGCTCTGTCATGGTTGCCTGAACATATC
AGTTGCTATAGCTATCACAA

Downstream 100 bases:

>100_bases
ATAGTTATAAAAAAACCTTTCGAAGCACTTCTAAAGTACTGCTGCTCCAATAAGTTCTCTAGTGTACTTAGGCTTGTTTT
CATCTGTAAGACCCAAATTC

Product: ATP-dependent DNA helicase UvrD

Products: NA

Alternate protein names: ATP-dependent helicase/nuclease AddA [H]

Number of amino acids: Translated: 907; Mature: 907

Protein sequence:

>907_residues
MIEKVTDPNVSVWINSSAGCGKTTLLVRRAISLLVNKEKNILCITFTKVATAEMHNRIFAILGKLSAMNDTDMDEYLLST
INRTVKDRDYVRKLVYTADAFIQIQTLHSFCWQSIKMCDRRHLSKEIYEENQHMFCQLLARFIWERCQLSKKTAEKTTER
KLHETLASLLSNFHLDYRKIIEKLKNDPLEQLATESIDILSAFTQFLEINQNISKHYISHNGIIETAIEMEIWNVYEIAA
KIDHILLDEAQDNSIKQWYIISNLCRDFLIDETHNKSIFVVGDYKQSIYGFQGASPEMYLAFYDILKQRDPQNKLVQVKT
AKSYRSSTAILQFVDSVFENIQLCKTSSEKIRHTTSRTEAPGYIEVIPLLQYGEVDAELQLAISIRETIQSWLKIGRTLK
AKNRPVKGSDILILVAHRTNLVDHIYTQLTAINIPVNFTGKFTVKNNPLFELLINLGKFLIYKHDDEALITLLKSPIFAW
SHSALLKLKAEKKKEWLFEQICATYAGKVLKRWLEFYGTTFDIYSQVLDARTVTTLAKYYGSETPYYIDIFLDNTLEHEC
IHDFIYIFSGEDATLKIKSKNENGVTISTVHGSKGTQSPIVFIVDSNYVPQNRDIFFFTEDGTPLLSSETHVRVVQEMRD
VKKESIRYEHLRLLYVALTRAEDELYIMGIGKQAKKNSWYEACSNGIVKIGSQNQDGAYYYTNDLLYPIKIKQKVDKSDE
NPSLHRERDSNRRMKNTQELQNKESDLANSYEFPYKRDETFDNGIIDDDVTKVSEKTFQIIRGKLIHKILEYISKVEKAE
KWMNLFLEEYSPELSNEQKNEIKRTVLLFLEKNHRADGKNEVEVYFDGKLLRMDNVKFEKDTITIRDYKTGKDKSVDLAI
EKQMHRYKEAMQAIYPEKNVITEIIWL

Sequences:

>Translated_907_residues
MIEKVTDPNVSVWINSSAGCGKTTLLVRRAISLLVNKEKNILCITFTKVATAEMHNRIFAILGKLSAMNDTDMDEYLLST
INRTVKDRDYVRKLVYTADAFIQIQTLHSFCWQSIKMCDRRHLSKEIYEENQHMFCQLLARFIWERCQLSKKTAEKTTER
KLHETLASLLSNFHLDYRKIIEKLKNDPLEQLATESIDILSAFTQFLEINQNISKHYISHNGIIETAIEMEIWNVYEIAA
KIDHILLDEAQDNSIKQWYIISNLCRDFLIDETHNKSIFVVGDYKQSIYGFQGASPEMYLAFYDILKQRDPQNKLVQVKT
AKSYRSSTAILQFVDSVFENIQLCKTSSEKIRHTTSRTEAPGYIEVIPLLQYGEVDAELQLAISIRETIQSWLKIGRTLK
AKNRPVKGSDILILVAHRTNLVDHIYTQLTAINIPVNFTGKFTVKNNPLFELLINLGKFLIYKHDDEALITLLKSPIFAW
SHSALLKLKAEKKKEWLFEQICATYAGKVLKRWLEFYGTTFDIYSQVLDARTVTTLAKYYGSETPYYIDIFLDNTLEHEC
IHDFIYIFSGEDATLKIKSKNENGVTISTVHGSKGTQSPIVFIVDSNYVPQNRDIFFFTEDGTPLLSSETHVRVVQEMRD
VKKESIRYEHLRLLYVALTRAEDELYIMGIGKQAKKNSWYEACSNGIVKIGSQNQDGAYYYTNDLLYPIKIKQKVDKSDE
NPSLHRERDSNRRMKNTQELQNKESDLANSYEFPYKRDETFDNGIIDDDVTKVSEKTFQIIRGKLIHKILEYISKVEKAE
KWMNLFLEEYSPELSNEQKNEIKRTVLLFLEKNHRADGKNEVEVYFDGKLLRMDNVKFEKDTITIRDYKTGKDKSVDLAI
EKQMHRYKEAMQAIYPEKNVITEIIWL
>Mature_907_residues
MIEKVTDPNVSVWINSSAGCGKTTLLVRRAISLLVNKEKNILCITFTKVATAEMHNRIFAILGKLSAMNDTDMDEYLLST
INRTVKDRDYVRKLVYTADAFIQIQTLHSFCWQSIKMCDRRHLSKEIYEENQHMFCQLLARFIWERCQLSKKTAEKTTER
KLHETLASLLSNFHLDYRKIIEKLKNDPLEQLATESIDILSAFTQFLEINQNISKHYISHNGIIETAIEMEIWNVYEIAA
KIDHILLDEAQDNSIKQWYIISNLCRDFLIDETHNKSIFVVGDYKQSIYGFQGASPEMYLAFYDILKQRDPQNKLVQVKT
AKSYRSSTAILQFVDSVFENIQLCKTSSEKIRHTTSRTEAPGYIEVIPLLQYGEVDAELQLAISIRETIQSWLKIGRTLK
AKNRPVKGSDILILVAHRTNLVDHIYTQLTAINIPVNFTGKFTVKNNPLFELLINLGKFLIYKHDDEALITLLKSPIFAW
SHSALLKLKAEKKKEWLFEQICATYAGKVLKRWLEFYGTTFDIYSQVLDARTVTTLAKYYGSETPYYIDIFLDNTLEHEC
IHDFIYIFSGEDATLKIKSKNENGVTISTVHGSKGTQSPIVFIVDSNYVPQNRDIFFFTEDGTPLLSSETHVRVVQEMRD
VKKESIRYEHLRLLYVALTRAEDELYIMGIGKQAKKNSWYEACSNGIVKIGSQNQDGAYYYTNDLLYPIKIKQKVDKSDE
NPSLHRERDSNRRMKNTQELQNKESDLANSYEFPYKRDETFDNGIIDDDVTKVSEKTFQIIRGKLIHKILEYISKVEKAE
KWMNLFLEEYSPELSNEQKNEIKRTVLLFLEKNHRADGKNEVEVYFDGKLLRMDNVKFEKDTITIRDYKTGKDKSVDLAI
EKQMHRYKEAMQAIYPEKNVITEIIWL

Specific function: The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. The AddA nuclease domai

COG id: COG1074

COG function: function code L; ATP-dependent exoDNAse (exonuclease V) beta subunit (contains helicase and exonuclease domains)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014152
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR011604
- InterPro:   IPR014016
- InterPro:   IPR011335 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 105510; Mature: 105510

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEKVTDPNVSVWINSSAGCGKTTLLVRRAISLLVNKEKNILCITFTKVATAEMHNRIFA
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHH
ILGKLSAMNDTDMDEYLLSTINRTVKDRDYVRKLVYTADAFIQIQTLHSFCWQSIKMCDR
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHH
RHLSKEIYEENQHMFCQLLARFIWERCQLSKKTAEKTTERKLHETLASLLSNFHLDYRKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
IEKLKNDPLEQLATESIDILSAFTQFLEINQNISKHYISHNGIIETAIEMEIWNVYEIAA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEEEHHHHHHHHHHHHH
KIDHILLDEAQDNSIKQWYIISNLCRDFLIDETHNKSIFVVGDYKQSIYGFQGASPEMYL
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHCCCCCCCCHHHH
AFYDILKQRDPQNKLVQVKTAKSYRSSTAILQFVDSVFENIQLCKTSSEKIRHTTSRTEA
HHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCC
PGYIEVIPLLQYGEVDAELQLAISIRETIQSWLKIGRTLKAKNRPVKGSDILILVAHRTN
CCEEEEEEEEECCCCCCCCEEEHHHHHHHHHHHHHCCHHHCCCCCCCCCCEEEEEECCHH
LVDHIYTQLTAINIPVNFTGKFTVKNNPLFELLINLGKFLIYKHDDEALITLLKSPIFAW
HHHHHHHHHEEEEEEEEECEEEEECCCCHHHHHHHHCCEEEEECCCHHHHHHHHCCHHHC
SHSALLKLKAEKKKEWLFEQICATYAGKVLKRWLEFYGTTFDIYSQVLDARTVTTLAKYY
CCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
GSETPYYIDIFLDNTLEHECIHDFIYIFSGEDATLKIKSKNENGVTISTVHGSKGTQSPI
CCCCCEEEEEEECCCCCHHHHHHEEEEEECCCEEEEEECCCCCCCEEEEEECCCCCCCCE
VFIVDSNYVPQNRDIFFFTEDGTPLLSSETHVRVVQEMRDVKKESIRYEHLRLLYVALTR
EEEEECCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AEDELYIMGIGKQAKKNSWYEACSNGIVKIGSQNQDGAYYYTNDLLYPIKIKQKVDKSDE
CCCCEEEEECCCHHHCCHHHHHHCCCEEEECCCCCCCCEEEECCEEEEEEEHHHCCCCCC
NPSLHRERDSNRRMKNTQELQNKESDLANSYEFPYKRDETFDNGIIDDDVTKVSEKTFQI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
IRGKLIHKILEYISKVEKAEKWMNLFLEEYSPELSNEQKNEIKRTVLLFLEKNHRADGKN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEECCCCCCCCC
EVEVYFDGKLLRMDNVKFEKDTITIRDYKTGKDKSVDLAIEKQMHRYKEAMQAIYPEKNV
CEEEEECCEEEEECCCEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHH
ITEIIWL
HHHHHCC
>Mature Secondary Structure
MIEKVTDPNVSVWINSSAGCGKTTLLVRRAISLLVNKEKNILCITFTKVATAEMHNRIFA
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHH
ILGKLSAMNDTDMDEYLLSTINRTVKDRDYVRKLVYTADAFIQIQTLHSFCWQSIKMCDR
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHH
RHLSKEIYEENQHMFCQLLARFIWERCQLSKKTAEKTTERKLHETLASLLSNFHLDYRKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
IEKLKNDPLEQLATESIDILSAFTQFLEINQNISKHYISHNGIIETAIEMEIWNVYEIAA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEEEHHHHHHHHHHHHH
KIDHILLDEAQDNSIKQWYIISNLCRDFLIDETHNKSIFVVGDYKQSIYGFQGASPEMYL
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHCCCCCCCCHHHH
AFYDILKQRDPQNKLVQVKTAKSYRSSTAILQFVDSVFENIQLCKTSSEKIRHTTSRTEA
HHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCC
PGYIEVIPLLQYGEVDAELQLAISIRETIQSWLKIGRTLKAKNRPVKGSDILILVAHRTN
CCEEEEEEEEECCCCCCCCEEEHHHHHHHHHHHHHCCHHHCCCCCCCCCCEEEEEECCHH
LVDHIYTQLTAINIPVNFTGKFTVKNNPLFELLINLGKFLIYKHDDEALITLLKSPIFAW
HHHHHHHHHEEEEEEEEECEEEEECCCCHHHHHHHHCCEEEEECCCHHHHHHHHCCHHHC
SHSALLKLKAEKKKEWLFEQICATYAGKVLKRWLEFYGTTFDIYSQVLDARTVTTLAKYY
CCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
GSETPYYIDIFLDNTLEHECIHDFIYIFSGEDATLKIKSKNENGVTISTVHGSKGTQSPI
CCCCCEEEEEEECCCCCHHHHHHEEEEEECCCEEEEEECCCCCCCEEEEEECCCCCCCCE
VFIVDSNYVPQNRDIFFFTEDGTPLLSSETHVRVVQEMRDVKKESIRYEHLRLLYVALTR
EEEEECCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AEDELYIMGIGKQAKKNSWYEACSNGIVKIGSQNQDGAYYYTNDLLYPIKIKQKVDKSDE
CCCCEEEEECCCHHHCCHHHHHHCCCEEEECCCCCCCCEEEECCEEEEEEEHHHCCCCCC
NPSLHRERDSNRRMKNTQELQNKESDLANSYEFPYKRDETFDNGIIDDDVTKVSEKTFQI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
IRGKLIHKILEYISKVEKAEKWMNLFLEEYSPELSNEQKNEIKRTVLLFLEKNHRADGKN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEECCCCCCCCC
EVEVYFDGKLLRMDNVKFEKDTITIRDYKTGKDKSVDLAIEKQMHRYKEAMQAIYPEKNV
CEEEEECCEEEEECCCEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHH
ITEIIWL
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA