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The map label for this gene is trmD

Identifier: 88608142

GI number: 88608142

Start: 799941

End: 800618

Strand: Reverse

Name: trmD

Synonym: NSE_0896

Alternate gene names: 88608142

Gene position: 800618-799941 (Counterclockwise)

Preceding gene: 88608812

Following gene: 88608669

Centisome position: 93.2

GC content: 48.38

Gene sequence:

>678_bases
GTGCGCTTTGTCGTCCTGACAATGTTTCCAGAGGTTTTTCCTGGGCCTTTGGGCGTGTCTGTAATCGGTAGAGGGTTGGC
CAATGGGTATTGGTCCCTTGAAGTGATTCCGATAAGGCGTTTTGCTGTGAATAGTCGCGTTGATGATGCTCCTTACGGTG
GGGGACCTGGTATGGTGATGCGTGCCGATGTACTGGGAGAGGCGTTTGAGTATGCGAACTCCACCTATCAAATAAAGAGA
CGTATATTTCTCAGTCCTAGGGGTACGAAATTCGTACAGAACTCTACCGCTAATTTAGTGGAAGCTGATAATGTCCTGTT
AATTTGTGGGCGCTTCGAAGGAGTTGACCAAAGATTCTTGAATTACTACAATGTTGAAGAGCTCAGTGTCGGTGATTACG
TACTTTCTGGTGGTGAGATTGCTGCCATGGCGGTGATAGACAGTTGTGTCAGGTGTATTCCCGGGGTTTTGGGCAATCTG
CAGAGTCTCGAGTGTGAGAGCTTTGTTGGTTCCTCGTTGGAGTACGACCATTATACGCGGCCGAACTCTTGGAAAGGATT
GAGTGTCCCTGAGGTTCTTATGAGTGGGAACCACAGGAAGATAAGTGAGTGGAGGGTTAACTCCGCTGCAACCGCAACGG
GTAGAAATAGGCCGGATTTGGTTGAAGAAGAAAGTTAG

Upstream 100 bases:

>100_bases
CGATCATACCATTTTCGCTATGAAGTCTGGCTCTGTATCGTTTTCTGTCGGTCTCAGGGGTCGGCGTTTTGTTCACGTGG
TATAGTAGACCGTTTCTGTT

Downstream 100 bases:

>100_bases
GTTTGATTTTATAACAAGTCGATATGGGTATCTTAGCTAAATTTAATGAGGCTCAGAGGAAAGCTCTTTCTGAAGGTAGG
AACTATCCCGAATTCAGAGC

Product: tRNA (guanine-N1)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MRFVVLTMFPEVFPGPLGVSVIGRGLANGYWSLEVIPIRRFAVNSRVDDAPYGGGPGMVMRADVLGEAFEYANSTYQIKR
RIFLSPRGTKFVQNSTANLVEADNVLLICGRFEGVDQRFLNYYNVEELSVGDYVLSGGEIAAMAVIDSCVRCIPGVLGNL
QSLECESFVGSSLEYDHYTRPNSWKGLSVPEVLMSGNHRKISEWRVNSAATATGRNRPDLVEEES

Sequences:

>Translated_225_residues
MRFVVLTMFPEVFPGPLGVSVIGRGLANGYWSLEVIPIRRFAVNSRVDDAPYGGGPGMVMRADVLGEAFEYANSTYQIKR
RIFLSPRGTKFVQNSTANLVEADNVLLICGRFEGVDQRFLNYYNVEELSVGDYVLSGGEIAAMAVIDSCVRCIPGVLGNL
QSLECESFVGSSLEYDHYTRPNSWKGLSVPEVLMSGNHRKISEWRVNSAATATGRNRPDLVEEES
>Mature_225_residues
MRFVVLTMFPEVFPGPLGVSVIGRGLANGYWSLEVIPIRRFAVNSRVDDAPYGGGPGMVMRADVLGEAFEYANSTYQIKR
RIFLSPRGTKFVQNSTANLVEADNVLLICGRFEGVDQRFLNYYNVEELSVGDYVLSGGEIAAMAVIDSCVRCIPGVLGNL
QSLECESFVGSSLEYDHYTRPNSWKGLSVPEVLMSGNHRKISEWRVNSAATATGRNRPDLVEEES

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=221, Percent_Identity=42.0814479638009, Blast_Score=171, Evalue=5e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_NEOSM (Q2GCN4)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506762.1
- ProteinModelPortal:   Q2GCN4
- SMR:   Q2GCN4
- STRING:   Q2GCN4
- GeneID:   3931442
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0896
- TIGR:   NSE_0896
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   CLSK2527740
- BioCyc:   NSEN222891:NSE_0896-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 24826; Mature: 24826

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: BINDING 110-110

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFVVLTMFPEVFPGPLGVSVIGRGLANGYWSLEVIPIRRFAVNSRVDDAPYGGGPGMVM
CEEEEEEECHHHCCCCCCHHHHHHHHCCCEEEEEEEEEEEEEECCCCCCCCCCCCCCCEE
RADVLGEAFEYANSTYQIKRRIFLSPRGTKFVQNSTANLVEADNVLLICGRFEGVDQRFL
EHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCEEECCCEEEEEECCCCHHHHHH
NYYNVEELSVGDYVLSGGEIAAMAVIDSCVRCIPGVLGNLQSLECESFVGSSLEYDHYTR
HHCCCCEECCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCC
PNSWKGLSVPEVLMSGNHRKISEWRVNSAATATGRNRPDLVEEES
CCCCCCCCHHHHHHCCCCCCHHHEECCCCEECCCCCCCCCCCCCC
>Mature Secondary Structure
MRFVVLTMFPEVFPGPLGVSVIGRGLANGYWSLEVIPIRRFAVNSRVDDAPYGGGPGMVM
CEEEEEEECHHHCCCCCCHHHHHHHHCCCEEEEEEEEEEEEEECCCCCCCCCCCCCCCEE
RADVLGEAFEYANSTYQIKRRIFLSPRGTKFVQNSTANLVEADNVLLICGRFEGVDQRFL
EHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCEEECCCEEEEEECCCCHHHHHH
NYYNVEELSVGDYVLSGGEIAAMAVIDSCVRCIPGVLGNLQSLECESFVGSSLEYDHYTR
HHCCCCEECCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCC
PNSWKGLSVPEVLMSGNHRKISEWRVNSAATATGRNRPDLVEEES
CCCCCCCCHHHHHHCCCCCCHHHEECCCCEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA