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The map label for this gene is nuoB

Identifier: 88608136

GI number: 88608136

Start: 352952

End: 353455

Strand: Direct

Name: nuoB

Synonym: NSE_0431

Alternate gene names: 88608136

Gene position: 352952-353455 (Clockwise)

Preceding gene: 88608004

Following gene: 88608330

Centisome position: 41.09

GC content: 44.84

Gene sequence:

>504_bases
ATGGGAAATGGTTTTAGTGATGTAAACGCACTTTCTAATGTTAGCAGTGATGGTTATCTCGTTGCTAAACTTGACGACCT
TATAAATTGGGCTAGGTCGGGTTCTTTGTGGCCGATGACTTTTGGTTTGGCGTGTTGTGCAGTTGAGATGATGCATTTTA
CTGCTAGTCGATATGATATGGATAGATTTGGGATGATTTTCAGGCCGAGTCCAAGGCAGTCTGATGTTATGATTGTTGCA
GGTACCCTGACCAACAAGATGGCACCAGCACTCCGCAGGGTGTACGATCAAATGGCCGAGCCCAAGTATGTAATCTCAAT
GGGGAGTTGTGCTAATGGAGGGGGATACTATCACTACTCATATTCTGTCGTAAGAGGTTGCGATAGGATTGTGCCCGTGG
ATGTATATGTTCCTGGTTGTCCGCCTACAGCAGAAGCGCTTTTGTACGGTGTTCTGTGCCTTCAGAAAAAAATAAACCGG
CAGAGAAACTTTATCAGGAGATAA

Upstream 100 bases:

>100_bases
TGGTATCAGTTATTGTTTTAGCTTCTTGCATTGGGTTGTGTCGGTTTCAGTAGGTTGTAGGAGTAATCTAGGCTATTTTT
GTTCAATTACTTGGTGCTTT

Downstream 100 bases:

>100_bases
ACCGATTTACCGAATATGACTATTGACACAACTGAAGTTGATGCTCTAATTAATGCGCCTGGGCGGTGTGTTGTTAATTC
TTCGCTGGAGTCTTTGGTTG

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MGNGFSDVNALSNVSSDGYLVAKLDDLINWARSGSLWPMTFGLACCAVEMMHFTASRYDMDRFGMIFRPSPRQSDVMIVA
GTLTNKMAPALRRVYDQMAEPKYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGVLCLQKKINR
QRNFIRR

Sequences:

>Translated_167_residues
MGNGFSDVNALSNVSSDGYLVAKLDDLINWARSGSLWPMTFGLACCAVEMMHFTASRYDMDRFGMIFRPSPRQSDVMIVA
GTLTNKMAPALRRVYDQMAEPKYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGVLCLQKKINR
QRNFIRR
>Mature_166_residues
GNGFSDVNALSNVSSDGYLVAKLDDLINWARSGSLWPMTFGLACCAVEMMHFTASRYDMDRFGMIFRPSPRQSDVMIVAG
TLTNKMAPALRRVYDQMAEPKYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGVLCLQKKINRQ
RNFIRR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=150, Percent_Identity=82.6666666666667, Blast_Score=273, Evalue=4e-74,
Organism=Escherichia coli, GI1788624, Length=156, Percent_Identity=50, Blast_Score=172, Evalue=9e-45,
Organism=Escherichia coli, GI1789074, Length=118, Percent_Identity=41.5254237288136, Blast_Score=103, Evalue=7e-24,
Organism=Escherichia coli, GI1788834, Length=122, Percent_Identity=37.7049180327869, Blast_Score=94, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17509685, Length=153, Percent_Identity=73.8562091503268, Blast_Score=255, Evalue=1e-68,
Organism=Drosophila melanogaster, GI18859983, Length=146, Percent_Identity=78.0821917808219, Blast_Score=262, Evalue=8e-71,
Organism=Drosophila melanogaster, GI24642371, Length=146, Percent_Identity=78.0821917808219, Blast_Score=262, Evalue=8e-71,
Organism=Drosophila melanogaster, GI24651058, Length=146, Percent_Identity=78.0821917808219, Blast_Score=258, Evalue=2e-69,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_NEOSM (Q2GDX9)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506317.1
- HSSP:   Q56218
- ProteinModelPortal:   Q2GDX9
- SMR:   Q2GDX9
- STRING:   Q2GDX9
- GeneID:   3932128
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0431
- TIGR:   NSE_0431
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   GKPIGSN
- PhylomeDB:   Q2GDX9
- ProtClustDB:   PRK06411
- BioCyc:   NSEN222891:NSE_0431-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 18617; Mature: 18486

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
9.6 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
9.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNGFSDVNALSNVSSDGYLVAKLDDLINWARSGSLWPMTFGLACCAVEMMHFTASRYDM
CCCCHHHHHHHHCCCCCCEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCH
DRFGMIFRPSPRQSDVMIVAGTLTNKMAPALRRVYDQMAEPKYVISMGSCANGGGYYHYS
HHCCEEECCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEE
YSVVRGCDRIVPVDVYVPGCPPTAEALLYGVLCLQKKINRQRNFIRR
HHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GNGFSDVNALSNVSSDGYLVAKLDDLINWARSGSLWPMTFGLACCAVEMMHFTASRYDM
CCCHHHHHHHHCCCCCCEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCH
DRFGMIFRPSPRQSDVMIVAGTLTNKMAPALRRVYDQMAEPKYVISMGSCANGGGYYHYS
HHCCEEECCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEE
YSVVRGCDRIVPVDVYVPGCPPTAEALLYGVLCLQKKINRQRNFIRR
HHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA