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The map label for this gene is miaA

Identifier: 88608118

GI number: 88608118

Start: 495140

End: 496057

Strand: Reverse

Name: miaA

Synonym: NSE_0570

Alternate gene names: 88608118

Gene position: 496057-495140 (Counterclockwise)

Preceding gene: 88608764

Following gene: 88608228

Centisome position: 57.75

GC content: 43.36

Gene sequence:

>918_bases
ATGCCCAATGCAAGTCGGAGGGTTCTTGTTCTTACAGGGCCTACTTCCTCTGGGAAGACTTCTGTTGCATGTGCGGTCAG
TCAGGTGTCTGATGATCTTGTGATTATTAATGCCGACTCGAAACAGGTTTATAGGGAGTTACCCATTTTGACCAGTCAGG
CTTCTTGCGGAATGCTTTACGGGTATTTGTCGGTTTTTGATGAGTTTATTCCATCAGTTGCTTCTTGGATGCGTGATTGT
GCTGATTGTCTCGAGAGTGTCTGGGCGCAGAAAGGTATACCTTTAATTGTTGGTGGGACCCCAATGTATCTGTATTCCCT
GCTGCATGGGATTAATCTTCTTCCTTCCTTGCCCGATTGTCTGATGCGGGAGCTTTCGGAGGAACTTGATAATCTTGGTC
CTTCTGGTTTTCTTAATCGTTTTGTTTGTAAATCAGGTGAGGACTTGTCCCGGTTTTCATGCGATTCGTATAAGATGCTT
AGGGATGTTGCATACTTCCTATACACTGGTAAAACGATTCAAGAGTTGTATCGAGAATCTTCCGTATACAAGATTCCATA
TGACTCCATTGACGTTGTAGCTATTATCCCGTCGGATAGAGATTCTCTCTACTCCAAAATAAATGAGAGGTTTCTTGAAG
CAGTGAATTCAGGGGCGATAGATGAGGTAGCCTCAGTTGTTGAAAATAAAGCGGCTTTTAGGAACCGTGCGGTGACAACC
ATATGTGGTTTTAGAGAGATAGCCTCATATTTAAGGGATGAAATCACCCTCGAGCGCATGGTTGCGATGGGTCAGCGAAG
TATTCGTAATTATGCTAAAAGGCAGTTAACCTGGTTTAGAAACAAGTTCAATGGAATTAAAGCTTTCGACCAACCTCAGG
ATGCTGAGAGGTACATTTTAAGGGAAATACTTCTCTGA

Upstream 100 bases:

>100_bases
GGTGTGAGAAGGGGTAAAGGAGGTTCGCAGCAAGAAGAGCACGACTCTGAGGAGATTCACCAGAAGGAAGAGGAGCTGGT
ACAGTAGGAGTCACTTTGCC

Downstream 100 bases:

>100_bases
CGTCTAGCATAGTTGCAAAACTAAATGAAGGGAATTACTCGGAAAAGCTAGTTTCACATAAAATTAGCCACTTGGACTTT
CCTTGTGATTGGTGCGGTCT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MPNASRRVLVLTGPTSSGKTSVACAVSQVSDDLVIINADSKQVYRELPILTSQASCGMLYGYLSVFDEFIPSVASWMRDC
ADCLESVWAQKGIPLIVGGTPMYLYSLLHGINLLPSLPDCLMRELSEELDNLGPSGFLNRFVCKSGEDLSRFSCDSYKML
RDVAYFLYTGKTIQELYRESSVYKIPYDSIDVVAIIPSDRDSLYSKINERFLEAVNSGAIDEVASVVENKAAFRNRAVTT
ICGFREIASYLRDEITLERMVAMGQRSIRNYAKRQLTWFRNKFNGIKAFDQPQDAERYILREILL

Sequences:

>Translated_305_residues
MPNASRRVLVLTGPTSSGKTSVACAVSQVSDDLVIINADSKQVYRELPILTSQASCGMLYGYLSVFDEFIPSVASWMRDC
ADCLESVWAQKGIPLIVGGTPMYLYSLLHGINLLPSLPDCLMRELSEELDNLGPSGFLNRFVCKSGEDLSRFSCDSYKML
RDVAYFLYTGKTIQELYRESSVYKIPYDSIDVVAIIPSDRDSLYSKINERFLEAVNSGAIDEVASVVENKAAFRNRAVTT
ICGFREIASYLRDEITLERMVAMGQRSIRNYAKRQLTWFRNKFNGIKAFDQPQDAERYILREILL
>Mature_304_residues
PNASRRVLVLTGPTSSGKTSVACAVSQVSDDLVIINADSKQVYRELPILTSQASCGMLYGYLSVFDEFIPSVASWMRDCA
DCLESVWAQKGIPLIVGGTPMYLYSLLHGINLLPSLPDCLMRELSEELDNLGPSGFLNRFVCKSGEDLSRFSCDSYKMLR
DVAYFLYTGKTIQELYRESSVYKIPYDSIDVVAIIPSDRDSLYSKINERFLEAVNSGAIDEVASVVENKAAFRNRAVTTI
CGFREIASYLRDEITLERMVAMGQRSIRNYAKRQLTWFRNKFNGIKAFDQPQDAERYILREILL

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Escherichia coli, GI1790613, Length=303, Percent_Identity=28.0528052805281, Blast_Score=110, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324848, Length=297, Percent_Identity=23.9057239057239, Blast_Score=89, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_NEOSM (Q2GDJ5)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506451.1
- ProteinModelPortal:   Q2GDJ5
- SMR:   Q2GDJ5
- STRING:   Q2GDJ5
- GeneID:   3932110
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0570
- TIGR:   NSE_0570
- eggNOG:   COG0324
- OMA:   FREIASY
- ProtClustDB:   CLSK2527722
- BioCyc:   NSEN222891:NSE_0570-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34371; Mature: 34240

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNASRRVLVLTGPTSSGKTSVACAVSQVSDDLVIINADSKQVYRELPILTSQASCGMLY
CCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHCCCCCCCCHHHHHH
GYLSVFDEFIPSVASWMRDCADCLESVWAQKGIPLIVGGTPMYLYSLLHGINLLPSLPDC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCCCHHH
LMRELSEELDNLGPSGFLNRFVCKSGEDLSRFSCDSYKMLRDVAYFLYTGKTIQELYRES
HHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHCC
SVYKIPYDSIDVVAIIPSDRDSLYSKINERFLEAVNSGAIDEVASVVENKAAFRNRAVTT
CEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
ICGFREIASYLRDEITLERMVAMGQRSIRNYAKRQLTWFRNKFNGIKAFDQPQDAERYIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
REILL
HHHCC
>Mature Secondary Structure 
PNASRRVLVLTGPTSSGKTSVACAVSQVSDDLVIINADSKQVYRELPILTSQASCGMLY
CCCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHCCCCCCCCHHHHHH
GYLSVFDEFIPSVASWMRDCADCLESVWAQKGIPLIVGGTPMYLYSLLHGINLLPSLPDC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCCCHHH
LMRELSEELDNLGPSGFLNRFVCKSGEDLSRFSCDSYKMLRDVAYFLYTGKTIQELYRES
HHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHCC
SVYKIPYDSIDVVAIIPSDRDSLYSKINERFLEAVNSGAIDEVASVVENKAAFRNRAVTT
CEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
ICGFREIASYLRDEITLERMVAMGQRSIRNYAKRQLTWFRNKFNGIKAFDQPQDAERYIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
REILL
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA