Click here to switch to the map view.

The map label for this gene is cutE

Identifier: 88608115

GI number: 88608115

Start: 415378

End: 416796

Strand: Reverse

Name: cutE

Synonym: NSE_0497

Alternate gene names: 88608115

Gene position: 416796-415378 (Counterclockwise)

Preceding gene: 88608382

Following gene: 88608327

Centisome position: 48.52

GC content: 38.48

Gene sequence:

>1419_bases
ATGAAGGTATCCGAAAAATACTTATCAGTACTCCTAGGAGTGTGTATGACGCTTGGGTTTGCACCCTTTCACCTAACACT
CATTCTGGTTTTTGCGCTTGCACTTGTATATAGACAACTAAGGTTGTCTTCCTCTATGCAGGAAACCTTCTTGTTCTCTG
TCCATTTTGGAAGTGCGTTTTTTTTGACTTCTTTATATTGGATTGCTTTTGCATTGGCGACAGATATACAAAATTTATGG
TGGTTAATCATACCAGTTCCTATTGTACTTTCTCTCTTTCTTTCTCTTTATTTCGCGTGCGCCTTTACCCTATTTAAAGC
AATCGCAATACAAAATCCACTTAGCTTCTCTATCATAATAACAGGATCAGAGTACCTAAGAGAAGTGTGTTTTGGTGGTT
TCCCATGGAACCTCATTGGTTATTCATGGAATCATCTAGAGATTCTTCAGCTGACGTCACTTTTTGGTATCTTTGGACTT
ACCGCATTCACAACATTTATTTCTGGATCAGTTGGAGAACTTCTTCTCAAAGATTCAAAAAAAGCCAGAATCTACTTTGT
TTGTTCAGTCATTTTATTTATATCAGTTTTTTGCTTTGGCCTCAGACGTTTGAGCCTTTATCCAACTCAATATTCCTCAA
AAGTCGTGAGGATAGTACAGGCAAATATCTCTCATCAGCAAGTTTGGACACCTGAATTTCAAAAGATGGTACTCAAAAAG
TACATTCAATTAAGCAGGTCTAGACCTTCAGCAAAAATCACCTATTTCGTGTGGCCGGAATCATCATTACCGTTCCTTTT
CTCGGGACCTAAAACAGATGTAACGCTTCACGTGAGTCAAGCTCTTGGAAAACCTCTCGTAGCTGCCGGAACACGTAGAG
ATAATAACGCATTATTCAATTCAATTTTTGTACTTCGCGATGGGATCATCGCAGAATATTATGACAAGGTCAAACTTATC
CCATTTGGAGAATACATACCTCTCGAAAATATAATACCAGTAAAAAAGATTGTTAATGGAATGAGCAGTTTTACTTATGG
TGATGATAGTACGAAGGTGCTAGACAGTATAAAGGCAGCACCGATCCTCTGTTACGAAAGTATTTTTAGCACTTTTGTTG
GTCGAAAAAAGCCTGCTGAATGGATAGTTAACCTTACGAATGACAGTTGGCTTGGAAATAGCACTGCACGGTATCAGCAT
CTACAAATGGCACGTGTCAGAGCGATAGAGTATGGCCTCCCAGTTGTTAGATCGACTACTACTGGTATTTCTGCAGTCAT
TGACCCATATGGAAGAATTCTGGAAAAAATAGACTACTCCTCCGAAGGAGTGTTAGATATCGCAATACCTGAAAAACATA
AAAAGAAAACTTTCTACAGTTCACTCATCCAAAATATTAATAAACTCCGTTCCTGGTAA

Upstream 100 bases:

>100_bases
CCGTTGACATCAATGTTGAAGGTAGCCTGTGGAAACCAAGAATCTTCTTTGATAGTAAAACGATATTCGAGACTCTCAGG
GAAACAAAAGCAAATTACAC

Downstream 100 bases:

>100_bases
CCAAGATGTGCACAGAGCCAGCCAGTACACAATGGTTTCTACGTTATATCACCTCCGCGCTCTGTAACGCGCGATGCCCC
ACCGGTCGGTACGTGCCTAT

Product: apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: ALP N-acyltransferase [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MKVSEKYLSVLLGVCMTLGFAPFHLTLILVFALALVYRQLRLSSSMQETFLFSVHFGSAFFLTSLYWIAFALATDIQNLW
WLIIPVPIVLSLFLSLYFACAFTLFKAIAIQNPLSFSIIITGSEYLREVCFGGFPWNLIGYSWNHLEILQLTSLFGIFGL
TAFTTFISGSVGELLLKDSKKARIYFVCSVILFISVFCFGLRRLSLYPTQYSSKVVRIVQANISHQQVWTPEFQKMVLKK
YIQLSRSRPSAKITYFVWPESSLPFLFSGPKTDVTLHVSQALGKPLVAAGTRRDNNALFNSIFVLRDGIIAEYYDKVKLI
PFGEYIPLENIIPVKKIVNGMSSFTYGDDSTKVLDSIKAAPILCYESIFSTFVGRKKPAEWIVNLTNDSWLGNSTARYQH
LQMARVRAIEYGLPVVRSTTTGISAVIDPYGRILEKIDYSSEGVLDIAIPEKHKKKTFYSSLIQNINKLRSW

Sequences:

>Translated_472_residues
MKVSEKYLSVLLGVCMTLGFAPFHLTLILVFALALVYRQLRLSSSMQETFLFSVHFGSAFFLTSLYWIAFALATDIQNLW
WLIIPVPIVLSLFLSLYFACAFTLFKAIAIQNPLSFSIIITGSEYLREVCFGGFPWNLIGYSWNHLEILQLTSLFGIFGL
TAFTTFISGSVGELLLKDSKKARIYFVCSVILFISVFCFGLRRLSLYPTQYSSKVVRIVQANISHQQVWTPEFQKMVLKK
YIQLSRSRPSAKITYFVWPESSLPFLFSGPKTDVTLHVSQALGKPLVAAGTRRDNNALFNSIFVLRDGIIAEYYDKVKLI
PFGEYIPLENIIPVKKIVNGMSSFTYGDDSTKVLDSIKAAPILCYESIFSTFVGRKKPAEWIVNLTNDSWLGNSTARYQH
LQMARVRAIEYGLPVVRSTTTGISAVIDPYGRILEKIDYSSEGVLDIAIPEKHKKKTFYSSLIQNINKLRSW
>Mature_472_residues
MKVSEKYLSVLLGVCMTLGFAPFHLTLILVFALALVYRQLRLSSSMQETFLFSVHFGSAFFLTSLYWIAFALATDIQNLW
WLIIPVPIVLSLFLSLYFACAFTLFKAIAIQNPLSFSIIITGSEYLREVCFGGFPWNLIGYSWNHLEILQLTSLFGIFGL
TAFTTFISGSVGELLLKDSKKARIYFVCSVILFISVFCFGLRRLSLYPTQYSSKVVRIVQANISHQQVWTPEFQKMVLKK
YIQLSRSRPSAKITYFVWPESSLPFLFSGPKTDVTLHVSQALGKPLVAAGTRRDNNALFNSIFVLRDGIIAEYYDKVKLI
PFGEYIPLENIIPVKKIVNGMSSFTYGDDSTKVLDSIKAAPILCYESIFSTFVGRKKPAEWIVNLTNDSWLGNSTARYQH
LQMARVRAIEYGLPVVRSTTTGISAVIDPYGRILEKIDYSSEGVLDIAIPEKHKKKTFYSSLIQNINKLRSW

Specific function: Transfers the fatty acyl group on membrane lipoproteins [H]

COG id: COG0815

COG function: function code M; Apolipoprotein N-acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1786878, Length=477, Percent_Identity=27.4633123689727, Blast_Score=136, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004563
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 2.3.1.- [C]

Molecular weight: Translated: 53457; Mature: 53457

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSEKYLSVLLGVCMTLGFAPFHLTLILVFALALVYRQLRLSSSMQETFLFSVHFGSAF
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEEEEECCHHH
FLTSLYWIAFALATDIQNLWWLIIPVPIVLSLFLSLYFACAFTLFKAIAIQNPLSFSIII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
TGSEYLREVCFGGFPWNLIGYSWNHLEILQLTSLFGIFGLTAFTTFISGSVGELLLKDSK
ECHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCC
KARIYFVCSVILFISVFCFGLRRLSLYPTQYSSKVVRIVQANISHQQVWTPEFQKMVLKK
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
YIQLSRSRPSAKITYFVWPESSLPFLFSGPKTDVTLHVSQALGKPLVAAGTRRDNNALFN
HHHHHCCCCCCEEEEEEECCCCCCEEECCCCCCEEEEEHHHHCCCCEECCCCCCCHHHHH
SIFVLRDGIIAEYYDKVKLIPFGEYIPLENIIPVKKIVNGMSSFTYGDDSTKVLDSIKAA
HHHHHHCCHHHHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCC
PILCYESIFSTFVGRKKPAEWIVNLTNDSWLGNSTARYQHLQMARVRAIEYGLPVVRSTT
HHHHHHHHHHHHHCCCCCHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHCCCC
TGISAVIDPYGRILEKIDYSSEGVLDIAIPEKHKKKTFYSSLIQNINKLRSW
CCHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVSEKYLSVLLGVCMTLGFAPFHLTLILVFALALVYRQLRLSSSMQETFLFSVHFGSAF
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEEEEECCHHH
FLTSLYWIAFALATDIQNLWWLIIPVPIVLSLFLSLYFACAFTLFKAIAIQNPLSFSIII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
TGSEYLREVCFGGFPWNLIGYSWNHLEILQLTSLFGIFGLTAFTTFISGSVGELLLKDSK
ECHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCC
KARIYFVCSVILFISVFCFGLRRLSLYPTQYSSKVVRIVQANISHQQVWTPEFQKMVLKK
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
YIQLSRSRPSAKITYFVWPESSLPFLFSGPKTDVTLHVSQALGKPLVAAGTRRDNNALFN
HHHHHCCCCCCEEEEEEECCCCCCEEECCCCCCEEEEEHHHHCCCCEECCCCCCCHHHHH
SIFVLRDGIIAEYYDKVKLIPFGEYIPLENIIPVKKIVNGMSSFTYGDDSTKVLDSIKAA
HHHHHHCCHHHHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCC
PILCYESIFSTFVGRKKPAEWIVNLTNDSWLGNSTARYQHLQMARVRAIEYGLPVVRSTT
HHHHHHHHHHHHHCCCCCHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHCCCC
TGISAVIDPYGRILEKIDYSSEGVLDIAIPEKHKKKTFYSSLIQNINKLRSW
CCHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA