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The map label for this gene is hisS

Identifier: 88608104

GI number: 88608104

Start: 693305

End: 694546

Strand: Reverse

Name: hisS

Synonym: NSE_0781

Alternate gene names: 88608104

Gene position: 694546-693305 (Counterclockwise)

Preceding gene: 88608831

Following gene: 88608243

Centisome position: 80.85

GC content: 40.18

Gene sequence:

>1242_bases
ATGAGTATAAAAATCAATAATGTCAAAGGGACTAGGGATCTGTTTGGTGAGCAGTTAGAAAAGATGCGTCTCATTGAGCA
GGTAGCCAAGAATCTTTCGATTCGGTATTTGTTTACTGAGCTTGAGACTCCGATAATTGAGCATACAGAGCTTTTTATTA
GGAACCTTGGTGAAACGTCAGACGTAGTAAATAAAGAGATCTATTCGTTTCAGGACAAAAGTGGTCACAATATTTGTCTA
AGACCAGAATTTACCGCTGCTGTCACTAGAGCATTCGTGGAGAATTTTCAGCATATTCAATCACCTGTTCGGTTATTTTC
TTTTGGTCCGCTATTCAGATATGAAAGACCACAAAAAGGGAGATATAGGCAATTTCATCAGGTGAATTTTGAATGGATCG
GAGCAAAGCATTATCTTTGGGCTGTTGAAGCTATAGTTTTAGCAAAGTCGTTCCTTAAAGAAATTGGAATAAGGTGTGAA
ATACGTGTTAATTCACTTGGTTGTTCTAGAACTCGTGAAGAGTATAAACTTGCACTCATCAACTATTTTCAACAGTACAA
AGAGCACCTTTCAGCTGATAGTTTGCTCAGATTGAAAAAGAATCCGTTGAGAATATTAGACTCGAAGGATCCATCTGAGA
AGGAAATTGTGGTGGGCGCGCCAAGAATTCTGGATTACCATACTGATGATGCTCTAAAGGAATTTGAATCAATTTGTGAT
ATACTGAAGCTCCTCGATATTGAGTTTTCTGTAGATCATAGGTTGGTCAGAGGATTAGATTATTATTCTGGTTTAATTTT
CGAATTTACTAGTCCTGATCTCGGTGCGCAGGATGCCCTCTTGGGAGGTGGAGCATATGAGCAACTTTCAGAGAATTTGG
GCGGAAAAAAAGTACAATCAATTGGGTTTGCTGCGGGGATTGAGCGTTTAATCGATATAATGCCAGTTTTGGCACCTACG
AGTGATAAGATCGTTTCGATTGTTCCCATCGGGGAAATTGCAGAAAGGGAGGCGCTAAAACTACTGTTTTACCTGCGCAG
TGAAGGATTATGCGCCGATATGTGCTATGGGCTCAGTGTTAAGTCGAGAATGAAACGTGCTGAAAGAAGCACAGTTACAG
TCATTCTTGGTGAGGAAGAATTTTCAAGGGGTGAGTCGACCGTAAGAATAATGGAGACTGGTCAACAAATGACTGTTGCG
CACGAAAAACTCCTATCAACATTGAGGGAATTGCTCTGTTGA

Upstream 100 bases:

>100_bases
GGTCTATCAAAAATTTCTAGACACAGAATGGTTTTCAAAATACTTGAGGCGCACTTCAAACTTGGCTTGCATGCGTTATC
TATGAAACTATGGACAGAGG

Downstream 100 bases:

>100_bases
AGGTTGTTCTAGATGAGTTGCATCTGTTAGGGTGCCAGTAGAGATTCCAAATATTAATCCTGGTATCACACAGCAAGGTT
TGGCTCTCAAACGGGACGGT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MSIKINNVKGTRDLFGEQLEKMRLIEQVAKNLSIRYLFTELETPIIEHTELFIRNLGETSDVVNKEIYSFQDKSGHNICL
RPEFTAAVTRAFVENFQHIQSPVRLFSFGPLFRYERPQKGRYRQFHQVNFEWIGAKHYLWAVEAIVLAKSFLKEIGIRCE
IRVNSLGCSRTREEYKLALINYFQQYKEHLSADSLLRLKKNPLRILDSKDPSEKEIVVGAPRILDYHTDDALKEFESICD
ILKLLDIEFSVDHRLVRGLDYYSGLIFEFTSPDLGAQDALLGGGAYEQLSENLGGKKVQSIGFAAGIERLIDIMPVLAPT
SDKIVSIVPIGEIAEREALKLLFYLRSEGLCADMCYGLSVKSRMKRAERSTVTVILGEEEFSRGESTVRIMETGQQMTVA
HEKLLSTLRELLC

Sequences:

>Translated_413_residues
MSIKINNVKGTRDLFGEQLEKMRLIEQVAKNLSIRYLFTELETPIIEHTELFIRNLGETSDVVNKEIYSFQDKSGHNICL
RPEFTAAVTRAFVENFQHIQSPVRLFSFGPLFRYERPQKGRYRQFHQVNFEWIGAKHYLWAVEAIVLAKSFLKEIGIRCE
IRVNSLGCSRTREEYKLALINYFQQYKEHLSADSLLRLKKNPLRILDSKDPSEKEIVVGAPRILDYHTDDALKEFESICD
ILKLLDIEFSVDHRLVRGLDYYSGLIFEFTSPDLGAQDALLGGGAYEQLSENLGGKKVQSIGFAAGIERLIDIMPVLAPT
SDKIVSIVPIGEIAEREALKLLFYLRSEGLCADMCYGLSVKSRMKRAERSTVTVILGEEEFSRGESTVRIMETGQQMTVA
HEKLLSTLRELLC
>Mature_412_residues
SIKINNVKGTRDLFGEQLEKMRLIEQVAKNLSIRYLFTELETPIIEHTELFIRNLGETSDVVNKEIYSFQDKSGHNICLR
PEFTAAVTRAFVENFQHIQSPVRLFSFGPLFRYERPQKGRYRQFHQVNFEWIGAKHYLWAVEAIVLAKSFLKEIGIRCEI
RVNSLGCSRTREEYKLALINYFQQYKEHLSADSLLRLKKNPLRILDSKDPSEKEIVVGAPRILDYHTDDALKEFESICDI
LKLLDIEFSVDHRLVRGLDYYSGLIFEFTSPDLGAQDALLGGGAYEQLSENLGGKKVQSIGFAAGIERLIDIMPVLAPTS
DKIVSIVPIGEIAEREALKLLFYLRSEGLCADMCYGLSVKSRMKRAERSTVTVILGEEEFSRGESTVRIMETGQQMTVAH
EKLLSTLRELLC

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1788861, Length=423, Percent_Identity=36.4066193853428, Blast_Score=280, Evalue=1e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_NEOSM (Q2GCZ0)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506656.1
- ProteinModelPortal:   Q2GCZ0
- SMR:   Q2GCZ0
- STRING:   Q2GCZ0
- GeneID:   3931975
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0781
- TIGR:   NSE_0781
- eggNOG:   COG0124
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- PhylomeDB:   Q2GCZ0
- ProtClustDB:   CLSK2527712
- BioCyc:   NSEN222891:NSE_0781-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 47201; Mature: 47070

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKINNVKGTRDLFGEQLEKMRLIEQVAKNLSIRYLFTELETPIIEHTELFIRNLGETS
CEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHCCCHHHHHHHHHHHCCCHH
DVVNKEIYSFQDKSGHNICLRPEFTAAVTRAFVENFQHIQSPVRLFSFGPLFRYERPQKG
HHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCC
RYRQFHQVNFEWIGAKHYLWAVEAIVLAKSFLKEIGIRCEIRVNSLGCSRTREEYKLALI
CHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHH
NYFQQYKEHLSADSLLRLKKNPLRILDSKDPSEKEIVVGAPRILDYHTDDALKEFESICD
HHHHHHHHHCCHHHHHHHCCCCCEEECCCCCCCCEEEEECCHHEECCCHHHHHHHHHHHH
ILKLLDIEFSVDHRLVRGLDYYSGLIFEFTSPDLGAQDALLGGGAYEQLSENLGGKKVQS
HHHHHCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCHHHCCCCHHHHHHHCCCCHHHHH
IGFAAGIERLIDIMPVLAPTSDKIVSIVPIGEIAEREALKLLFYLRSEGLCADMCYGLSV
HHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHCCCH
KSRMKRAERSTVTVILGEEEFSRGESTVRIMETGQQMTVAHEKLLSTLRELLC
HHHHHHHCCCEEEEEECCHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SIKINNVKGTRDLFGEQLEKMRLIEQVAKNLSIRYLFTELETPIIEHTELFIRNLGETS
EEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHCCCHHHHHHHHHHHCCCHH
DVVNKEIYSFQDKSGHNICLRPEFTAAVTRAFVENFQHIQSPVRLFSFGPLFRYERPQKG
HHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCC
RYRQFHQVNFEWIGAKHYLWAVEAIVLAKSFLKEIGIRCEIRVNSLGCSRTREEYKLALI
CHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHH
NYFQQYKEHLSADSLLRLKKNPLRILDSKDPSEKEIVVGAPRILDYHTDDALKEFESICD
HHHHHHHHHCCHHHHHHHCCCCCEEECCCCCCCCEEEEECCHHEECCCHHHHHHHHHHHH
ILKLLDIEFSVDHRLVRGLDYYSGLIFEFTSPDLGAQDALLGGGAYEQLSENLGGKKVQS
HHHHHCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCHHHCCCCHHHHHHHCCCCHHHHH
IGFAAGIERLIDIMPVLAPTSDKIVSIVPIGEIAEREALKLLFYLRSEGLCADMCYGLSV
HHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHCCCH
KSRMKRAERSTVTVILGEEEFSRGESTVRIMETGQQMTVAHEKLLSTLRELLC
HHHHHHHCCCEEEEEECCHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA