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The map label for this gene is cca [H]

Identifier: 88608101

GI number: 88608101

Start: 566524

End: 567702

Strand: Direct

Name: cca [H]

Synonym: NSE_0646

Alternate gene names: 88608101

Gene position: 566524-567702 (Clockwise)

Preceding gene: 88608408

Following gene: 88608067

Centisome position: 65.95

GC content: 40.29

Gene sequence:

>1179_bases
ATGAAGGTCGAAACAAGCTGGACTGAGGACTTAAGAACCCTCGTTCAAATAATACGTAATAATGGCGGAGAAGCAAGATT
CGTTGGCGGCTGCGTGCGGGACCAGCTCTTAGGAAGAAAAATTAGCGATATTGATCTGGCTACAACCCTCAAACCAGAAG
AAGCGATAAGGGCACTCAAAACAAACGGCTTAGTAACTATTCCCACTGGGCTTAAGCACGGTACCTTCACTGTTCTAGTC
AATTCAAGACCAATTGAGATAACAACACTAAGGCAGGATCTACACTGCGATGGGCGTCACGCAACTGTTTCATTCACAGA
TAGCTGGAAAGAAGATGCTCAAAGACGGGATTTCACATTCAATGCAATGTATATAGACATCGAGGGTAATATTCATGATT
ATTTTTCCGGATTGGAAGATTTACAGAACAGACGATTAAAATTTGTCGGAGAACCTCATCAGCGAATCGAAGAAGATTAT
CTACGCATATTGAGAGCATTTCGCTTCCAGGCATCTATTTGCAAGACACCGCTCTCGGAAGAAATCCTTCATGCTTGCAC
AAAGTACAGAAAAAAAATCTCTTCACTTTCGGGTGAGAGGATCCAAGGAGAAATGTTTAAACTGCTTTCTTATGTGAACT
TCCTTAGCACAGTTTTCACAATGCAGGAAGCAAAAATAATAGACGAAGTTCTAGGTGCGAAAGTGTCCTTCTCAGGATTA
GATTATGAAAAAAGTCGGCTTATACAAGATCATATCGCGGCTTTGACGCTACTCATAAGGAATACAGAGAATAGCTATAA
CCTACAATGGCTGTATTCACGATGGAGAATCTCGAAAAAGGTACAAAAGGCTATTTCAATTCTTTCAGAAGAAAAAGAAA
TGCACTGTCCAAAAAAGACCCTGGCAAAGCTCGGCAATGAGCTCACAAGAAAACTGATTAACATCCTGTATGCAGAAAAT
AAACTGTCTGACATACAACGCAAAGATTTTATGTTGGTCACAAATAATTCTGAAAGACCAATTTTTCCGGTAATGGGTAG
GGATCTTATACCGCTGGGTTACACCGGACCCGAGATAGGAAAGAAATTGAAGCAATTAGAATATCTATGGAGAGAAAGTA
GTTGTGTCCTCAGTAAAAACGAGCTCCTTTTGATGGCAATTAGGAAACAAGTAAATTAA

Upstream 100 bases:

>100_bases
CCCGTAATGGACGCGTTTATTGCAGTAACCAAGAGTGCTAGAATCAGGAGTACAAGGATTAACCATTTTGGAGAGAAAAG
CTTCTTTAGTCGCATAGTCA

Downstream 100 bases:

>100_bases
TCCCATTTTTCAGATAACACAGAACTGCAAAACCCAGACTTAAAAAGTGAAACCTAGGAAATTTCTCCACACTAAAGCAC
CAACCAAAGTAGAAGATCAG

Product: polyA polymerase family protein

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MKVETSWTEDLRTLVQIIRNNGGEARFVGGCVRDQLLGRKISDIDLATTLKPEEAIRALKTNGLVTIPTGLKHGTFTVLV
NSRPIEITTLRQDLHCDGRHATVSFTDSWKEDAQRRDFTFNAMYIDIEGNIHDYFSGLEDLQNRRLKFVGEPHQRIEEDY
LRILRAFRFQASICKTPLSEEILHACTKYRKKISSLSGERIQGEMFKLLSYVNFLSTVFTMQEAKIIDEVLGAKVSFSGL
DYEKSRLIQDHIAALTLLIRNTENSYNLQWLYSRWRISKKVQKAISILSEEKEMHCPKKTLAKLGNELTRKLINILYAEN
KLSDIQRKDFMLVTNNSERPIFPVMGRDLIPLGYTGPEIGKKLKQLEYLWRESSCVLSKNELLLMAIRKQVN

Sequences:

>Translated_392_residues
MKVETSWTEDLRTLVQIIRNNGGEARFVGGCVRDQLLGRKISDIDLATTLKPEEAIRALKTNGLVTIPTGLKHGTFTVLV
NSRPIEITTLRQDLHCDGRHATVSFTDSWKEDAQRRDFTFNAMYIDIEGNIHDYFSGLEDLQNRRLKFVGEPHQRIEEDY
LRILRAFRFQASICKTPLSEEILHACTKYRKKISSLSGERIQGEMFKLLSYVNFLSTVFTMQEAKIIDEVLGAKVSFSGL
DYEKSRLIQDHIAALTLLIRNTENSYNLQWLYSRWRISKKVQKAISILSEEKEMHCPKKTLAKLGNELTRKLINILYAEN
KLSDIQRKDFMLVTNNSERPIFPVMGRDLIPLGYTGPEIGKKLKQLEYLWRESSCVLSKNELLLMAIRKQVN
>Mature_392_residues
MKVETSWTEDLRTLVQIIRNNGGEARFVGGCVRDQLLGRKISDIDLATTLKPEEAIRALKTNGLVTIPTGLKHGTFTVLV
NSRPIEITTLRQDLHCDGRHATVSFTDSWKEDAQRRDFTFNAMYIDIEGNIHDYFSGLEDLQNRRLKFVGEPHQRIEEDY
LRILRAFRFQASICKTPLSEEILHACTKYRKKISSLSGERIQGEMFKLLSYVNFLSTVFTMQEAKIIDEVLGAKVSFSGL
DYEKSRLIQDHIAALTLLIRNTENSYNLQWLYSRWRISKKVQKAISILSEEKEMHCPKKTLAKLGNELTRKLINILYAEN
KLSDIQRKDFMLVTNNSERPIFPVMGRDLIPLGYTGPEIGKKLKQLEYLWRESSCVLSKNELLLMAIRKQVN

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=399, Percent_Identity=34.0852130325814, Blast_Score=190, Evalue=2e-48,
Organism=Escherichia coli, GI87081691, Length=201, Percent_Identity=32.3383084577114, Blast_Score=92, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71995920, Length=418, Percent_Identity=29.9043062200957, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI281360112, Length=396, Percent_Identity=32.3232323232323, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI21357337, Length=396, Percent_Identity=32.3232323232323, Blast_Score=174, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 45206; Mature: 45206

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVETSWTEDLRTLVQIIRNNGGEARFVGGCVRDQLLGRKISDIDLATTLKPEEAIRALK
CCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHH
TNGLVTIPTGLKHGTFTVLVNSRPIEITTLRQDLHCDGRHATVSFTDSWKEDAQRRDFTF
CCCEEEECCCCCCCEEEEEECCCCEEEEEEHHHCCCCCCEEEEEECCCHHHHHHHCCEEE
NAMYIDIEGNIHDYFSGLEDLQNRRLKFVGEPHQRIEEDYLRILRAFRFQASICKTPLSE
EEEEEEEECCHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
EILHACTKYRKKISSLSGERIQGEMFKLLSYVNFLSTVFTMQEAKIIDEVLGAKVSFSGL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCC
DYEKSRLIQDHIAALTLLIRNTENSYNLQWLYSRWRISKKVQKAISILSEEKEMHCPKKT
CHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LAKLGNELTRKLINILYAENKLSDIQRKDFMLVTNNSERPIFPVMGRDLIPLGYTGPEIG
HHHHHHHHHHHHHHHHHHCCHHHHHHHCCEEEEECCCCCCEEECCCCCCCCCCCCCHHHH
KKLKQLEYLWRESSCVLSKNELLLMAIRKQVN
HHHHHHHHHHCCCCCEECCCCCHHHHHHHHCC
>Mature Secondary Structure
MKVETSWTEDLRTLVQIIRNNGGEARFVGGCVRDQLLGRKISDIDLATTLKPEEAIRALK
CCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHH
TNGLVTIPTGLKHGTFTVLVNSRPIEITTLRQDLHCDGRHATVSFTDSWKEDAQRRDFTF
CCCEEEECCCCCCCEEEEEECCCCEEEEEEHHHCCCCCCEEEEEECCCHHHHHHHCCEEE
NAMYIDIEGNIHDYFSGLEDLQNRRLKFVGEPHQRIEEDYLRILRAFRFQASICKTPLSE
EEEEEEEECCHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
EILHACTKYRKKISSLSGERIQGEMFKLLSYVNFLSTVFTMQEAKIIDEVLGAKVSFSGL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCC
DYEKSRLIQDHIAALTLLIRNTENSYNLQWLYSRWRISKKVQKAISILSEEKEMHCPKKT
CHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LAKLGNELTRKLINILYAENKLSDIQRKDFMLVTNNSERPIFPVMGRDLIPLGYTGPEIG
HHHHHHHHHHHHHHHHHHCCHHHHHHHCCEEEEECCCCCCEEECCCCCCCCCCCCCHHHH
KKLKQLEYLWRESSCVLSKNELLLMAIRKQVN
HHHHHHHHHHCCCCCEECCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA