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The map label for this gene is pyrF

Identifier: 88608051

GI number: 88608051

Start: 704640

End: 705326

Strand: Direct

Name: pyrF

Synonym: NSE_0793

Alternate gene names: 88608051

Gene position: 704640-705326 (Clockwise)

Preceding gene: 88608658

Following gene: 88608690

Centisome position: 82.03

GC content: 41.34

Gene sequence:

>687_bases
GTGAAACCAAAAATCATCTGTGCAGTGGATAATCCTGATTTTGGATATTCATACTCACTAGTGAGCAGCATATTGGAATA
CGTGCATGTCATAAAACTTGGGTTAGAGTTCTTTTCTTCATGCGGTATTGCAGGTATTGAGAAAGTTGCTGCTCTTGGGC
TGCCAATTTTTCTGGACCTCAAATTGCACGATATTCCCAATACAGTTGCTAGGACTATCAGCGTTATCAAACAGATTGAA
CGTGTTGAGTTCCTTACCGTCCATATCACTGGTGGTATGGAAATGCTTGCAGCTGCTAAGGAAGCACTTCAAGGTACTAA
GATACTCTTAATTGGGGTCTCTGTTCTGACAAGCCTCGTACAAAAGGATCTGGAAAATAACGGTATTGAGATTCCCCTGC
TAGAATACGTAAAAAAACTGTCTGTATTTGCAAAAAAATGCTGTCTAGACGGTGTCGTATGCTCAAACCAAGAAGCTCCC
ACGGTGCGAGATGCTTGTGGAGAGGAATTCATAATCGTCACGCCAGGAATTGCAAGTAAATACTCTGATGATCAAAAAAG
GGTCTCTCAAATTTCAGCACTAAAAAACAGCGTTTCCGATTATGTAGTCATTGGGAGGGCTATAACACGTGCATCAGATC
CGGTAAAAGCAACCAAGGAAATAATTTCTTCACTCACAAGGGAATAG

Upstream 100 bases:

>100_bases
AAATGTGTAAACTCGCATGTACAAATACTCGACTATGGATCGTGTAGAAAATCGAATACTGTGTTACATGTACCAAAATG
GATGCAGAAAGCTATAAAGA

Downstream 100 bases:

>100_bases
GCTGCCACAATATATTCAATGTGCACCGGTCAGAAGCACCGAAACTATTTCTCGTCAGTGCTCCTATGTTTTTGCGCAAC
ATTGCACAGTTGCACAATCA

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MKPKIICAVDNPDFGYSYSLVSSILEYVHVIKLGLEFFSSCGIAGIEKVAALGLPIFLDLKLHDIPNTVARTISVIKQIE
RVEFLTVHITGGMEMLAAAKEALQGTKILLIGVSVLTSLVQKDLENNGIEIPLLEYVKKLSVFAKKCCLDGVVCSNQEAP
TVRDACGEEFIIVTPGIASKYSDDQKRVSQISALKNSVSDYVVIGRAITRASDPVKATKEIISSLTRE

Sequences:

>Translated_228_residues
MKPKIICAVDNPDFGYSYSLVSSILEYVHVIKLGLEFFSSCGIAGIEKVAALGLPIFLDLKLHDIPNTVARTISVIKQIE
RVEFLTVHITGGMEMLAAAKEALQGTKILLIGVSVLTSLVQKDLENNGIEIPLLEYVKKLSVFAKKCCLDGVVCSNQEAP
TVRDACGEEFIIVTPGIASKYSDDQKRVSQISALKNSVSDYVVIGRAITRASDPVKATKEIISSLTRE
>Mature_228_residues
MKPKIICAVDNPDFGYSYSLVSSILEYVHVIKLGLEFFSSCGIAGIEKVAALGLPIFLDLKLHDIPNTVARTISVIKQIE
RVEFLTVHITGGMEMLAAAKEALQGTKILLIGVSVLTSLVQKDLENNGIEIPLLEYVKKLSVFAKKCCLDGVVCSNQEAP
TVRDACGEEFIIVTPGIASKYSDDQKRVSQISALKNSVSDYVVIGRAITRASDPVKATKEIISSLTRE

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=228, Percent_Identity=37.280701754386, Blast_Score=139, Evalue=1e-34,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 24744; Mature: 24744

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPKIICAVDNPDFGYSYSLVSSILEYVHVIKLGLEFFSSCGIAGIEKVAALGLPIFLDL
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEE
KLHDIPNTVARTISVIKQIERVEFLTVHITGGMEMLAAAKEALQGTKILLIGVSVLTSLV
EECCCCHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHCCCEEEEHHHHHHHHHH
QKDLENNGIEIPLLEYVKKLSVFAKKCCLDGVVCSNQEAPTVRDACGEEFIIVTPGIASK
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCEEEECCCCCCC
YSDDQKRVSQISALKNSVSDYVVIGRAITRASDPVKATKEIISSLTRE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKPKIICAVDNPDFGYSYSLVSSILEYVHVIKLGLEFFSSCGIAGIEKVAALGLPIFLDL
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEE
KLHDIPNTVARTISVIKQIERVEFLTVHITGGMEMLAAAKEALQGTKILLIGVSVLTSLV
EECCCCHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHCCCEEEEHHHHHHHHHH
QKDLENNGIEIPLLEYVKKLSVFAKKCCLDGVVCSNQEAPTVRDACGEEFIIVTPGIASK
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCEEEECCCCCCC
YSDDQKRVSQISALKNSVSDYVVIGRAITRASDPVKATKEIISSLTRE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA