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The map label for this gene is tgt

Identifier: 88608023

GI number: 88608023

Start: 13098

End: 14306

Strand: Reverse

Name: tgt

Synonym: NSE_0016

Alternate gene names: 88608023

Gene position: 14306-13098 (Counterclockwise)

Preceding gene: 88608792

Following gene: 88608689

Centisome position: 1.67

GC content: 39.78

Gene sequence:

>1209_bases
ATGCACGAATCAGAAGTTTTCCCTCGATTCAATTACCTAGAAAATGGAAAAACCCGCTCGAGGAGAGGTAAAATAACAAC
TAAGCATGGAGTGATACACACACCAGCTTTCATATTTTGTGCAACCCGAGCAGCAATAAAAGGCTTGGAAATACACCAAG
TCAAAGAAGCCGGATCTCAAGTAATACTGTCAAATACCTACCACTTGATGCTTAAACCCGGAGGAGAGCATATAGAAAAA
GCCGGTGGAATACAGAAGTTCATGGGGTGGAATGGACCAATGTTAACCGATTCTGGAGGTTTTCAAGCCTTCAGCCTAAA
TACAAAAGATGAGACAAATATACAAGAACTGAAAAGAAAATACTCCAAGGCATTCAACAGTTCCGGCATTCTTGCAATAT
CAGAAGAAGGAATAACCTGCAAATCTTACTACGATGGAGCAAAGTTTTTCCTATCCCCTGAAAAATCGATGGACGTTCAA
ATAAAACTCGGAGCAGACCTAATTGTTGTGCTGGATGAATGTACTAAATTCTCGTTGGATAGAGAATACAATAAGGAAGC
CCTAGAAAGAAATAAAAGATGGGCAGACAGGAGTCTCACATACGCAGAAAAAAATCGAAAAGCATGGCAAGGAGTCTACG
GAATTATACATGGAGGAACCTTTGAAGATTTAAGAAAGGAAAGCGTCGAGTTCAACAACAGTCGGGAACTTTTTGGAAAT
GCAATAGGTGGCTCTCTTGGAAGAACTAAAGAAGAAATGAAAGAGGTCGTGGAGATGTCTGTAACAAACCTAAACAGAGA
AAGACCTATTCATCTACTTGGAATAGGTGGCATATCTGATATTTTTACGGGTGTTAGTATGGGAGTGGACACCTTTGATT
GTGTCCACCCAACTAGAATAGCCCGGCATGGAGGAGCTCTAATAAAATACTCTTCCCACCCAGAAAACAACGGCAGGGAA
TACATAAATCTCAGAAACAATATCTTCAAAAACTCCGATTCATCAATCGATGAATCCTGCGATTGTAAAATCTGTGAGAC
TTACTCTTGCGGCTACCTAAACTATTTGCTCTCTGTCAAAGAGAACATCGCGTTCAATATGATCACCTACCACAACATAT
ACTTTATGAATAAACTCATGGAAAGAATAAGACACACAATAGAGAAGGACTTAGATCTGGAAGAGCTAAAAAAACTCTAC
TGTGGATAA

Upstream 100 bases:

>100_bases
CGTTCAAAAAGATCTTAGATGCTTAGTCTTTCCACTTTTTGGTGCGAAACGCTCGTGAAGCTATAATAAAACTCTGCAAA
TAGTGAGTACTTAAGACGAA

Downstream 100 bases:

>100_bases
GTGATCTTTCTCAGAAACTACCCTTTCAGTTACCACAATGAAAATCATTGGGACGGATTTGAGATTTATCTGAATTCGAA
AAACGCTTTTTTCGCGTAAG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MHESEVFPRFNYLENGKTRSRRGKITTKHGVIHTPAFIFCATRAAIKGLEIHQVKEAGSQVILSNTYHLMLKPGGEHIEK
AGGIQKFMGWNGPMLTDSGGFQAFSLNTKDETNIQELKRKYSKAFNSSGILAISEEGITCKSYYDGAKFFLSPEKSMDVQ
IKLGADLIVVLDECTKFSLDREYNKEALERNKRWADRSLTYAEKNRKAWQGVYGIIHGGTFEDLRKESVEFNNSRELFGN
AIGGSLGRTKEEMKEVVEMSVTNLNRERPIHLLGIGGISDIFTGVSMGVDTFDCVHPTRIARHGGALIKYSSHPENNGRE
YINLRNNIFKNSDSSIDESCDCKICETYSCGYLNYLLSVKENIAFNMITYHNIYFMNKLMERIRHTIEKDLDLEELKKLY
CG

Sequences:

>Translated_402_residues
MHESEVFPRFNYLENGKTRSRRGKITTKHGVIHTPAFIFCATRAAIKGLEIHQVKEAGSQVILSNTYHLMLKPGGEHIEK
AGGIQKFMGWNGPMLTDSGGFQAFSLNTKDETNIQELKRKYSKAFNSSGILAISEEGITCKSYYDGAKFFLSPEKSMDVQ
IKLGADLIVVLDECTKFSLDREYNKEALERNKRWADRSLTYAEKNRKAWQGVYGIIHGGTFEDLRKESVEFNNSRELFGN
AIGGSLGRTKEEMKEVVEMSVTNLNRERPIHLLGIGGISDIFTGVSMGVDTFDCVHPTRIARHGGALIKYSSHPENNGRE
YINLRNNIFKNSDSSIDESCDCKICETYSCGYLNYLLSVKENIAFNMITYHNIYFMNKLMERIRHTIEKDLDLEELKKLY
CG
>Mature_402_residues
MHESEVFPRFNYLENGKTRSRRGKITTKHGVIHTPAFIFCATRAAIKGLEIHQVKEAGSQVILSNTYHLMLKPGGEHIEK
AGGIQKFMGWNGPMLTDSGGFQAFSLNTKDETNIQELKRKYSKAFNSSGILAISEEGITCKSYYDGAKFFLSPEKSMDVQ
IKLGADLIVVLDECTKFSLDREYNKEALERNKRWADRSLTYAEKNRKAWQGVYGIIHGGTFEDLRKESVEFNNSRELFGN
AIGGSLGRTKEEMKEVVEMSVTNLNRERPIHLLGIGGISDIFTGVSMGVDTFDCVHPTRIARHGGALIKYSSHPENNGRE
YINLRNNIFKNSDSSIDESCDCKICETYSCGYLNYLLSVKENIAFNMITYHNIYFMNKLMERIRHTIEKDLDLEELKKLY
CG

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=386, Percent_Identity=36.2694300518135, Blast_Score=250, Evalue=2e-66,
Organism=Escherichia coli, GI1786607, Length=380, Percent_Identity=35.2631578947368, Blast_Score=249, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17544680, Length=372, Percent_Identity=31.7204301075269, Blast_Score=204, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI25146502, Length=138, Percent_Identity=26.8115942028986, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI19920510, Length=388, Percent_Identity=32.7319587628866, Blast_Score=233, Evalue=2e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 45617; Mature: 45617

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHESEVFPRFNYLENGKTRSRRGKITTKHGVIHTPAFIFCATRAAIKGLEIHQVKEAGSQ
CCCCCCCCCCCHHCCCCCCCCCCCEEECCCEEECCHHHHHHHHHHHCCCHHHHHHHCCCE
VILSNTYHLMLKPGGEHIEKAGGIQKFMGWNGPMLTDSGGFQAFSLNTKDETNIQELKRK
EEEECCEEEEECCCHHHHHHHCCHHHHHCCCCCEEECCCCEEEEEECCCCCCCHHHHHHH
YSKAFNSSGILAISEEGITCKSYYDGAKFFLSPEKSMDVQIKLGADLIVVLDECTKFSLD
HHHHCCCCCEEEECCCCCEEEEECCCCEEEECCCCCCCEEEEECCCEEEEEEHHHHHCCC
REYNKEALERNKRWADRSLTYAEKNRKAWQGVYGIIHGGTFEDLRKESVEFNNSRELFGN
HHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCCCHHHHHHHHCCCCCCHHHHHH
AIGGSLGRTKEEMKEVVEMSVTNLNRERPIHLLGIGGISDIFTGVSMGVDTFDCVHPTRI
HHCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHCCCCCHHCCCHHHH
ARHGGALIKYSSHPENNGREYINLRNNIFKNSDSSIDESCDCKICETYSCGYLNYLLSVK
HHCCCEEEEECCCCCCCCCCEEEEHHHHCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHH
ENIAFNMITYHNIYFMNKLMERIRHTIEKDLDLEELKKLYCG
HCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure
MHESEVFPRFNYLENGKTRSRRGKITTKHGVIHTPAFIFCATRAAIKGLEIHQVKEAGSQ
CCCCCCCCCCCHHCCCCCCCCCCCEEECCCEEECCHHHHHHHHHHHCCCHHHHHHHCCCE
VILSNTYHLMLKPGGEHIEKAGGIQKFMGWNGPMLTDSGGFQAFSLNTKDETNIQELKRK
EEEECCEEEEECCCHHHHHHHCCHHHHHCCCCCEEECCCCEEEEEECCCCCCCHHHHHHH
YSKAFNSSGILAISEEGITCKSYYDGAKFFLSPEKSMDVQIKLGADLIVVLDECTKFSLD
HHHHCCCCCEEEECCCCCEEEEECCCCEEEECCCCCCCEEEEECCCEEEEEEHHHHHCCC
REYNKEALERNKRWADRSLTYAEKNRKAWQGVYGIIHGGTFEDLRKESVEFNNSRELFGN
HHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCCCHHHHHHHHCCCCCCHHHHHH
AIGGSLGRTKEEMKEVVEMSVTNLNRERPIHLLGIGGISDIFTGVSMGVDTFDCVHPTRI
HHCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHCCCCCHHCCCHHHH
ARHGGALIKYSSHPENNGREYINLRNNIFKNSDSSIDESCDCKICETYSCGYLNYLLSVK
HHCCCEEEEECCCCCCCCCCEEEEHHHHCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHH
ENIAFNMITYHNIYFMNKLMERIRHTIEKDLDLEELKKLYCG
HCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA