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The map label for this gene is prfA

Identifier: 88608013

GI number: 88608013

Start: 329625

End: 330728

Strand: Direct

Name: prfA

Synonym: NSE_0406

Alternate gene names: 88608013

Gene position: 329625-330728 (Clockwise)

Preceding gene: 88608114

Following gene: 88608386

Centisome position: 38.37

GC content: 42.21

Gene sequence:

>1104_bases
ATGGATAATCTTTGGGGAGTTCTGGAAGAGATTCTAAATAGGCATACATACCTTGCAGACAGGTTACGCTGTCCGGAAGC
TATAGCTTCTCAGGAGTTTTCAAAGCTTTCTAAGGAGTATGCGGAGCTTAAGCCAAAAGTTGAATTAATTGCTAGATATA
AAAAATTGAAAGAAGAACAGGCCTATCTAGAACTGTTGGTAAAAAATCCACTGGAGGATCAGGAGATACGTGAAATAGGG
AAGTCTGATCTGCAAGCAATCCAAGATGTGATACCAAAGCTCGAGTTAGAAATAAAAAGGATGTTGCTACCAAAGGATAA
AGATGACGCTTTGAACGTCATGCTGGAAGTGAGGGCTGGCACCGGTGGTGACGAAGCTGCACTTTTTGCAGCGAGTCTCT
TCCATATGTATCAAAAATATGCAGAGCGCATGAAGTGGCGCTTTGAGATCATTAGTATTTCCCATAATGAGATAGGTGGA
TACAAAGAAGCCAATGCTAGTATAAGTGGATCTGATGTTTTCGCAAAGTTAAAGTTTGAGTCTGGCGTGCACAGGGTTCA
GCGTGTTCCAGAAACGGAGAGTGCTGGACGTATACATACTTCAACAGCCACTGTTGCTGTACTCCCAGAACCCGAAGATG
TAGATGTAAAAATCAACGATAAGGATCTACGTATCGATGTGTATCGGTCTAGTGGTCCTGGAGGACAGTCTGTCAACACA
ACTGACAGTGCAGTTCGTATTACACACATTCCGACCGGCATAGTAGTTATACAACAAGACGAGAAATCCCAACATAAGAA
CCGTGCTAAGGCAATGAAGGTGCTCAGAGTAAGATTGTATGAAATAGAGCGCAATAAAGTTCAACAAGAGATTTCGTCAA
TGAGAAAGTCGCAGATTGGATCGGGTGAACGCTCTGAAAAAACTAGAACATACAATTTCCCACAGGGCAGGATAACTGAT
CACAGGATCAATTTAACTACTTATAGGATTAATGAAACGGTAAAGGAGGGCGAACTGGAACCAATTATTGAAGCACTTAT
TAGAGAAAATGAAGCTCGTATGTTAGCTGGTGTCGAGGTAGGCTTCAGCGGAGATAAGTCTTGA

Upstream 100 bases:

>100_bases
AAAATAAGTTTTGTGCGTGCTATAACTTTAGCACTGCCAGTAAAGGGGTCTAGCATAGAGCGCTGTAACTTTGGAGGGCT
AGCAAATCTTAAGAGGGTTT

Downstream 100 bases:

>100_bases
GTGTTCTTAAGATCTGTTTTCTAATGAGTGTTATTCTGCTGAATGCAGTTTTTGAACGGTTTTCAGGTGGATCTTTTCTG
TTGTTCGTTATGCACTACAA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MDNLWGVLEEILNRHTYLADRLRCPEAIASQEFSKLSKEYAELKPKVELIARYKKLKEEQAYLELLVKNPLEDQEIREIG
KSDLQAIQDVIPKLELEIKRMLLPKDKDDALNVMLEVRAGTGGDEAALFAASLFHMYQKYAERMKWRFEIISISHNEIGG
YKEANASISGSDVFAKLKFESGVHRVQRVPETESAGRIHTSTATVAVLPEPEDVDVKINDKDLRIDVYRSSGPGGQSVNT
TDSAVRITHIPTGIVVIQQDEKSQHKNRAKAMKVLRVRLYEIERNKVQQEISSMRKSQIGSGERSEKTRTYNFPQGRITD
HRINLTTYRINETVKEGELEPIIEALIRENEARMLAGVEVGFSGDKS

Sequences:

>Translated_367_residues
MDNLWGVLEEILNRHTYLADRLRCPEAIASQEFSKLSKEYAELKPKVELIARYKKLKEEQAYLELLVKNPLEDQEIREIG
KSDLQAIQDVIPKLELEIKRMLLPKDKDDALNVMLEVRAGTGGDEAALFAASLFHMYQKYAERMKWRFEIISISHNEIGG
YKEANASISGSDVFAKLKFESGVHRVQRVPETESAGRIHTSTATVAVLPEPEDVDVKINDKDLRIDVYRSSGPGGQSVNT
TDSAVRITHIPTGIVVIQQDEKSQHKNRAKAMKVLRVRLYEIERNKVQQEISSMRKSQIGSGERSEKTRTYNFPQGRITD
HRINLTTYRINETVKEGELEPIIEALIRENEARMLAGVEVGFSGDKS
>Mature_367_residues
MDNLWGVLEEILNRHTYLADRLRCPEAIASQEFSKLSKEYAELKPKVELIARYKKLKEEQAYLELLVKNPLEDQEIREIG
KSDLQAIQDVIPKLELEIKRMLLPKDKDDALNVMLEVRAGTGGDEAALFAASLFHMYQKYAERMKWRFEIISISHNEIGG
YKEANASISGSDVFAKLKFESGVHRVQRVPETESAGRIHTSTATVAVLPEPEDVDVKINDKDLRIDVYRSSGPGGQSVNT
TDSAVRITHIPTGIVVIQQDEKSQHKNRAKAMKVLRVRLYEIERNKVQQEISSMRKSQIGSGERSEKTRTYNFPQGRITD
HRINLTTYRINETVKEGELEPIIEALIRENEARMLAGVEVGFSGDKS

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=304, Percent_Identity=47.3684210526316, Blast_Score=273, Evalue=1e-73,
Organism=Homo sapiens, GI34577120, Length=349, Percent_Identity=43.2664756446991, Blast_Score=253, Evalue=3e-67,
Organism=Homo sapiens, GI166795305, Length=208, Percent_Identity=48.0769230769231, Blast_Score=201, Evalue=8e-52,
Organism=Escherichia coli, GI1787462, Length=353, Percent_Identity=53.2577903682719, Blast_Score=351, Evalue=4e-98,
Organism=Escherichia coli, GI2367172, Length=317, Percent_Identity=40.0630914826498, Blast_Score=202, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17542784, Length=295, Percent_Identity=40.3389830508475, Blast_Score=214, Evalue=6e-56,
Organism=Saccharomyces cerevisiae, GI6321295, Length=322, Percent_Identity=44.0993788819876, Blast_Score=234, Evalue=2e-62,
Organism=Drosophila melanogaster, GI19921226, Length=277, Percent_Identity=45.8483754512635, Blast_Score=239, Evalue=2e-63,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_NEOSM (Q2GE03)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506293.1
- ProteinModelPortal:   Q2GE03
- SMR:   Q2GE03
- STRING:   Q2GE03
- GeneID:   3931950
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0406
- TIGR:   NSE_0406
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- PhylomeDB:   Q2GE03
- ProtClustDB:   CLSK2527657
- BioCyc:   NSEN222891:NSE_0406-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 41692; Mature: 41692

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNLWGVLEEILNRHTYLADRLRCPEAIASQEFSKLSKEYAELKPKVELIARYKKLKEEQ
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
AYLELLVKNPLEDQEIREIGKSDLQAIQDVIPKLELEIKRMLLPKDKDDALNVMLEVRAG
HHHHHHHCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECC
TGGDEAALFAASLFHMYQKYAERMKWRFEIISISHNEIGGYKEANASISGSDVFAKLKFE
CCCCHHHHHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEHH
SGVHRVQRVPETESAGRIHTSTATVAVLPEPEDVDVKINDKDLRIDVYRSSGPGGQSVNT
HHHHHHHCCCCCCCCCEEEECCEEEEEECCCCCCEEEECCCEEEEEEEECCCCCCCCCCC
TDSAVRITHIPTGIVVIQQDEKSQHKNRAKAMKVLRVRLYEIERNKVQQEISSMRKSQIG
CCCEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SGERSEKTRTYNFPQGRITDHRINLTTYRINETVKEGELEPIIEALIRENEARMLAGVEV
CCCCCCCCCEECCCCCCCCCEEEEEEEEEECCHHHCCCHHHHHHHHHCCCCCCEEEEEEE
GFSGDKS
CCCCCCC
>Mature Secondary Structure
MDNLWGVLEEILNRHTYLADRLRCPEAIASQEFSKLSKEYAELKPKVELIARYKKLKEEQ
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
AYLELLVKNPLEDQEIREIGKSDLQAIQDVIPKLELEIKRMLLPKDKDDALNVMLEVRAG
HHHHHHHCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECC
TGGDEAALFAASLFHMYQKYAERMKWRFEIISISHNEIGGYKEANASISGSDVFAKLKFE
CCCCHHHHHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEHH
SGVHRVQRVPETESAGRIHTSTATVAVLPEPEDVDVKINDKDLRIDVYRSSGPGGQSVNT
HHHHHHHCCCCCCCCCEEEECCEEEEEECCCCCCEEEECCCEEEEEEEECCCCCCCCCCC
TDSAVRITHIPTGIVVIQQDEKSQHKNRAKAMKVLRVRLYEIERNKVQQEISSMRKSQIG
CCCEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SGERSEKTRTYNFPQGRITDHRINLTTYRINETVKEGELEPIIEALIRENEARMLAGVEV
CCCCCCCCCEECCCCCCCCCEEEEEEEEEECCHHHCCCHHHHHHHHHCCCCCCEEEEEEE
GFSGDKS
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA