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The map label for this gene is pheT [H]

Identifier: 88607984

GI number: 88607984

Start: 347553

End: 350183

Strand: Direct

Name: pheT [H]

Synonym: NSE_0426

Alternate gene names: 88607984

Gene position: 347553-350183 (Clockwise)

Preceding gene: 88608153

Following gene: 88608795

Centisome position: 40.46

GC content: 39.83

Gene sequence:

>2631_bases
ATGAAATTCACTTTTGGGTGGTTATTAGAACATCTAGATACGACACTTTCTTTAGAGCAAGTTGTGGAAGCACTGATATC
TTTGGGTTTTGAGGTGGAAAATTGTCACTCGATAGGGAGGGGTTTTATCGTAGCACAGGTTACTGAATGTAATAAGCATC
CAGGAGCAGATAGATTGACTCTCTGCAAGGTTTTTGATGGTACGCAAGAACTACAGATTGTCTGCGGTGCACCAAATGTA
AGGGGTGGTCTTAAAGTTGTACTTGCGCAGGTTGGTGCAGTTCTACCGTGCAATATGATGAAAATTAAGAAGAGTAAAAT
CAGAGGATGCGAGAGCCTAGGAATGCTCTGTTCTCTAGAGGAGTTAGGACTTCCTGGAGGGGATAAGGGAGGTATTATTG
AGCTCTCTGATGATTATAGAGTTGGGGATGCTTTCACAGTTGATCCACTTATTGAAATTGCTGTCACACCTAACAGGGGC
GATATGTTGAGTGTATATGGCGTGGCAAGGGAATTAGCTGCAGGGGGATACGGTGTTCTCATTGGCACGTATAAGCCATC
CTTTGATGGCTTATACACGGAACGTAACCATGAAAGCGGGTGGCATGACTCTTGTGAAGTAGAGAACATTACACCTAATC
ATGGTAGCAATGTGTTAAGTTCTCCTCTCACTGATTCTCAAGGCAGCGCAGTGGTTGATGAATTAGCTAGTAATCTCCTT
AAGAAGGATGCGAATGTAGAAGATGATGCCAATATTCTATTTAATATTTGTGACTCCACTGCCTGCCGAAAATTTTCTGG
ATTAGTGATCAGAAATGTGAAAAATTGCGTCTCTCCAGACTGGCTTAGGAATAAGGTGGAAAGAGTAGGTGTAAAATCAA
TTTCTGCTCTCGTGGATATAGCAAACTATGTAATGTTCTCATACGGGTTACCATTACACATTTATGATCTGGACAAAATT
GCTGGTGAGACTCTACAAGTATCCGTATCAGATGAAGAATCCTACTTGGCCGCTTTAAATGATAAGGAATATCGCGTGCC
ACCTGGGTCCATAATAATTAGGGACCGAGATATGCAATTGCAGGCAGTTGCTGGAATTATAGGAAGTAAATTAGGTGCTT
GTACATTGTCGACACGAAATATTTTTATAGAAGCAGCTGTGTTTAATCCAGTAATGGTAGCTTCTACGAAAAGGGCTATG
AAAATTAATACAGAATCTGCTTATCGATTTGAACGTGGTGTCGATCCATATTTTACAGAGAAAGCACTAGTGCTTGCCGC
TCAGCTGATATCTGATGTTTGTGCTGGGGAAAAAGAGAAAATAGAGAGCTTTTTCACCAAAGATGAGAGCAAATTCATAG
ATTTTTCTTTATCGTTTTTTAGCAAACTCACTGGTATAGAGCTCGATTCAAGTAAGGCAATTCAGATCCTTAAAGGACTG
GGATTTGAAGTTCATGAGATGCGTATGCCTCATTACCGCACTTGCAGCGAGTCGCATTCTGATAACACTAACGTCGGGAA
TAGAAAAAATTTAGCTACTGAAGATTCCATAGGCAATACTCATGTTTCAACTGCTGAGGTTGGGGTTAGTTCAAAAAAGG
ATTTGCAGATTTTCAGAGTTGTTCCGCCTAGTTGGCGTAGCGATGTTATCACTCAGGCATGTATAGCTGAAGAGCTTCTC
AGAGTATATGGTTATGCTCAACTTCGTAGTACTCCTCTGAAAAGTAAGATTCCAGTAATTTTCGAGACTTCCAAGGAAGA
TAGAATCAGAGAATTATTATTGAACAGGGGTTTTTCTGAGGTAGTAACGTGGCCGTTCATATCAGAGAAGAAGTGTTCTG
CTAATGAGAATATCTTGCGTATAAAAAATCCATTAGGTGCTGAGTTCAATGTTATGCGACCTAGTATCATTCCTAATTTG
GTCGAAGTTGCCCAAAGGAATATCAAAAACGGGCTTCACGTCATCAAGATTTTTGAAATAGGTAAAATTTATTCAACTAT
CTGTGAGAAAAAGATGCTTGCAGGGCTAATATATGGTGACTCATTTCCTAGAAATATGCATGAAAAAGCTCATAAGATGG
ATTTCTTTGACGCAAAAGCCGATGTAGAAGCACTTCTAACCTCTTTAGATTTAGGTGGGAGATACTCTTTTGAAAAATGC
GATGGGCCGTCCTTATATCACCCTGGAAAAGTGTCTAATGTGAAAATCGAAGGCAAAGAGGTTGGTTTCATAGGGGAATT
GCATCCAAGGGTTGCGTCAAATAGGCAGATTACCATATTTGAGTTATTTCTAGATAAACTTGCTTGCAATCTTAAATTAA
AGGAAAACACTTTTGGCAAATTTCCGATTGTCGAAAGGGATTTTTCCTTCGTATTTGATCTGGACAAAGTGCCACTTTGG
GGTGATATTGAGAGGGGTTTGTACTTGATCAGTGATTTCATCAAAAATGTGACTCTCTTTGATAAGTATGAAGGTGATTT
TAAGGGAAAACGGATGTTGTCATTAGCCTTTTTCGTGAGTATGGAAAACCAAACGCATGGTTTATCGCAGGAAGAGATAA
AAAGTCTCAGCGATCAAATTATATGTTTTGTACAGGGAAAGTTCTCGGGAACCTTGCGTTTAAGTTGTTGA

Upstream 100 bases:

>100_bases
AGATCAGCGTACTTAACGCAGCGATATTCGACGTAAAAGAGCTTTCATAAATGGTGCAATATCATGTACCTTTTAATGGC
TCCATTATCTTTTCCTTTGT

Downstream 100 bases:

>100_bases
GTATCTCAAATAGCATAAGTGTAAAGTTAATCCGAATATGGTAACGGGGATTCTGAGGTGAATTTTACTAAAATATGGAA
ATAAGATTGCAGTCATTGCA

Product: putative phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 876; Mature: 876

Protein sequence:

>876_residues
MKFTFGWLLEHLDTTLSLEQVVEALISLGFEVENCHSIGRGFIVAQVTECNKHPGADRLTLCKVFDGTQELQIVCGAPNV
RGGLKVVLAQVGAVLPCNMMKIKKSKIRGCESLGMLCSLEELGLPGGDKGGIIELSDDYRVGDAFTVDPLIEIAVTPNRG
DMLSVYGVARELAAGGYGVLIGTYKPSFDGLYTERNHESGWHDSCEVENITPNHGSNVLSSPLTDSQGSAVVDELASNLL
KKDANVEDDANILFNICDSTACRKFSGLVIRNVKNCVSPDWLRNKVERVGVKSISALVDIANYVMFSYGLPLHIYDLDKI
AGETLQVSVSDEESYLAALNDKEYRVPPGSIIIRDRDMQLQAVAGIIGSKLGACTLSTRNIFIEAAVFNPVMVASTKRAM
KINTESAYRFERGVDPYFTEKALVLAAQLISDVCAGEKEKIESFFTKDESKFIDFSLSFFSKLTGIELDSSKAIQILKGL
GFEVHEMRMPHYRTCSESHSDNTNVGNRKNLATEDSIGNTHVSTAEVGVSSKKDLQIFRVVPPSWRSDVITQACIAEELL
RVYGYAQLRSTPLKSKIPVIFETSKEDRIRELLLNRGFSEVVTWPFISEKKCSANENILRIKNPLGAEFNVMRPSIIPNL
VEVAQRNIKNGLHVIKIFEIGKIYSTICEKKMLAGLIYGDSFPRNMHEKAHKMDFFDAKADVEALLTSLDLGGRYSFEKC
DGPSLYHPGKVSNVKIEGKEVGFIGELHPRVASNRQITIFELFLDKLACNLKLKENTFGKFPIVERDFSFVFDLDKVPLW
GDIERGLYLISDFIKNVTLFDKYEGDFKGKRMLSLAFFVSMENQTHGLSQEEIKSLSDQIICFVQGKFSGTLRLSC

Sequences:

>Translated_876_residues
MKFTFGWLLEHLDTTLSLEQVVEALISLGFEVENCHSIGRGFIVAQVTECNKHPGADRLTLCKVFDGTQELQIVCGAPNV
RGGLKVVLAQVGAVLPCNMMKIKKSKIRGCESLGMLCSLEELGLPGGDKGGIIELSDDYRVGDAFTVDPLIEIAVTPNRG
DMLSVYGVARELAAGGYGVLIGTYKPSFDGLYTERNHESGWHDSCEVENITPNHGSNVLSSPLTDSQGSAVVDELASNLL
KKDANVEDDANILFNICDSTACRKFSGLVIRNVKNCVSPDWLRNKVERVGVKSISALVDIANYVMFSYGLPLHIYDLDKI
AGETLQVSVSDEESYLAALNDKEYRVPPGSIIIRDRDMQLQAVAGIIGSKLGACTLSTRNIFIEAAVFNPVMVASTKRAM
KINTESAYRFERGVDPYFTEKALVLAAQLISDVCAGEKEKIESFFTKDESKFIDFSLSFFSKLTGIELDSSKAIQILKGL
GFEVHEMRMPHYRTCSESHSDNTNVGNRKNLATEDSIGNTHVSTAEVGVSSKKDLQIFRVVPPSWRSDVITQACIAEELL
RVYGYAQLRSTPLKSKIPVIFETSKEDRIRELLLNRGFSEVVTWPFISEKKCSANENILRIKNPLGAEFNVMRPSIIPNL
VEVAQRNIKNGLHVIKIFEIGKIYSTICEKKMLAGLIYGDSFPRNMHEKAHKMDFFDAKADVEALLTSLDLGGRYSFEKC
DGPSLYHPGKVSNVKIEGKEVGFIGELHPRVASNRQITIFELFLDKLACNLKLKENTFGKFPIVERDFSFVFDLDKVPLW
GDIERGLYLISDFIKNVTLFDKYEGDFKGKRMLSLAFFVSMENQTHGLSQEEIKSLSDQIICFVQGKFSGTLRLSC
>Mature_876_residues
MKFTFGWLLEHLDTTLSLEQVVEALISLGFEVENCHSIGRGFIVAQVTECNKHPGADRLTLCKVFDGTQELQIVCGAPNV
RGGLKVVLAQVGAVLPCNMMKIKKSKIRGCESLGMLCSLEELGLPGGDKGGIIELSDDYRVGDAFTVDPLIEIAVTPNRG
DMLSVYGVARELAAGGYGVLIGTYKPSFDGLYTERNHESGWHDSCEVENITPNHGSNVLSSPLTDSQGSAVVDELASNLL
KKDANVEDDANILFNICDSTACRKFSGLVIRNVKNCVSPDWLRNKVERVGVKSISALVDIANYVMFSYGLPLHIYDLDKI
AGETLQVSVSDEESYLAALNDKEYRVPPGSIIIRDRDMQLQAVAGIIGSKLGACTLSTRNIFIEAAVFNPVMVASTKRAM
KINTESAYRFERGVDPYFTEKALVLAAQLISDVCAGEKEKIESFFTKDESKFIDFSLSFFSKLTGIELDSSKAIQILKGL
GFEVHEMRMPHYRTCSESHSDNTNVGNRKNLATEDSIGNTHVSTAEVGVSSKKDLQIFRVVPPSWRSDVITQACIAEELL
RVYGYAQLRSTPLKSKIPVIFETSKEDRIRELLLNRGFSEVVTWPFISEKKCSANENILRIKNPLGAEFNVMRPSIIPNL
VEVAQRNIKNGLHVIKIFEIGKIYSTICEKKMLAGLIYGDSFPRNMHEKAHKMDFFDAKADVEALLTSLDLGGRYSFEKC
DGPSLYHPGKVSNVKIEGKEVGFIGELHPRVASNRQITIFELFLDKLACNLKLKENTFGKFPIVERDFSFVFDLDKVPLW
GDIERGLYLISDFIKNVTLFDKYEGDFKGKRMLSLAFFVSMENQTHGLSQEEIKSLSDQIICFVQGKFSGTLRLSC

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=908, Percent_Identity=28.4140969162996, Blast_Score=327, Evalue=2e-90,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 97257; Mature: 97257

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTFGWLLEHLDTTLSLEQVVEALISLGFEVENCHSIGRGFIVAQVTECNKHPGADRLT
CCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHCCCCEEEEEEECCCCCCCCCCEE
LCKVFDGTQELQIVCGAPNVRGGLKVVLAQVGAVLPCNMMKIKKSKIRGCESLGMLCSLE
EEEECCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHEEHH
ELGLPGGDKGGIIELSDDYRVGDAFTVDPLIEIAVTPNRGDMLSVYGVARELAAGGYGVL
HHCCCCCCCCCEEEECCCCCCCCEEEECCEEEEEECCCCCCEEHHHHHHHHHHCCCCEEE
IGTYKPSFDGLYTERNHESGWHDSCEVENITPNHGSNVLSSPLTDSQGSAVVDELASNLL
EECCCCCCCCCEECCCCCCCCCCCCEECCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHH
KKDANVEDDANILFNICDSTACRKFSGLVIRNVKNCVSPDWLRNKVERVGVKSISALVDI
HCCCCCCCCCHHHEEHHCCHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
ANYVMFSYGLPLHIYDLDKIAGETLQVSVSDEESYLAALNDKEYRVPPGSIIIRDRDMQL
HHHHHHHCCCCEEEEEHHHHCCCEEEEEECCCCCEEEEECCCCEECCCCCEEEECCCCHH
QAVAGIIGSKLGACTLSTRNIFIEAAVFNPVMVASTKRAMKINTESAYRFERGVDPYFTE
HHHHHHHHCCCCEEEEECCEEEEEEEECCCEEEECCCCEEEECCHHHHHHHCCCCCHHHH
KALVLAAQLISDVCAGEKEKIESFFTKDESKFIDFSLSFFSKLTGIELDSSKAIQILKGL
HHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEHHHHHHHCCCCCCCHHHHHHHHHC
GFEVHEMRMPHYRTCSESHSDNTNVGNRKNLATEDSIGNTHVSTAEVGVSSKKDLQIFRV
CCEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCEEEEE
VPPSWRSDVITQACIAEELLRVYGYAQLRSTPLKSKIPVIFETSKEDRIRELLLNRGFSE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHCCHHH
VVTWPFISEKKCSANENILRIKNPLGAEFNVMRPSIIPNLVEVAQRNIKNGLHVIKIFEI
HEECCCCCCCCCCCCCCEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHCCCEEEEEEHH
GKIYSTICEKKMLAGLIYGDSFPRNMHEKAHKMDFFDAKADVEALLTSLDLGGRYSFEKC
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCC
DGPSLYHPGKVSNVKIEGKEVGFIGELHPRVASNRQITIFELFLDKLACNLKLKENTFGK
CCCCCCCCCCCCEEEECCCEECEEECCCCHHCCCCCEEHHHHHHHHHHCCEEEECCCCCC
FPIVERDFSFVFDLDKVPLWGDIERGLYLISDFIKNVTLFDKYEGDFKGKRMLSLAFFVS
CCEEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCHHEEEEEEEEE
MENQTHGLSQEEIKSLSDQIICFVQGKFSGTLRLSC
ECCCCCCCCHHHHHHHHCEEEEEEECCCCCEEEECC
>Mature Secondary Structure
MKFTFGWLLEHLDTTLSLEQVVEALISLGFEVENCHSIGRGFIVAQVTECNKHPGADRLT
CCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHCCCCEEEEEEECCCCCCCCCCEE
LCKVFDGTQELQIVCGAPNVRGGLKVVLAQVGAVLPCNMMKIKKSKIRGCESLGMLCSLE
EEEECCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHEEHH
ELGLPGGDKGGIIELSDDYRVGDAFTVDPLIEIAVTPNRGDMLSVYGVARELAAGGYGVL
HHCCCCCCCCCEEEECCCCCCCCEEEECCEEEEEECCCCCCEEHHHHHHHHHHCCCCEEE
IGTYKPSFDGLYTERNHESGWHDSCEVENITPNHGSNVLSSPLTDSQGSAVVDELASNLL
EECCCCCCCCCEECCCCCCCCCCCCEECCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHH
KKDANVEDDANILFNICDSTACRKFSGLVIRNVKNCVSPDWLRNKVERVGVKSISALVDI
HCCCCCCCCCHHHEEHHCCHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
ANYVMFSYGLPLHIYDLDKIAGETLQVSVSDEESYLAALNDKEYRVPPGSIIIRDRDMQL
HHHHHHHCCCCEEEEEHHHHCCCEEEEEECCCCCEEEEECCCCEECCCCCEEEECCCCHH
QAVAGIIGSKLGACTLSTRNIFIEAAVFNPVMVASTKRAMKINTESAYRFERGVDPYFTE
HHHHHHHHCCCCEEEEECCEEEEEEEECCCEEEECCCCEEEECCHHHHHHHCCCCCHHHH
KALVLAAQLISDVCAGEKEKIESFFTKDESKFIDFSLSFFSKLTGIELDSSKAIQILKGL
HHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEHHHHHHHCCCCCCCHHHHHHHHHC
GFEVHEMRMPHYRTCSESHSDNTNVGNRKNLATEDSIGNTHVSTAEVGVSSKKDLQIFRV
CCEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCEEEEE
VPPSWRSDVITQACIAEELLRVYGYAQLRSTPLKSKIPVIFETSKEDRIRELLLNRGFSE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHCCHHH
VVTWPFISEKKCSANENILRIKNPLGAEFNVMRPSIIPNLVEVAQRNIKNGLHVIKIFEI
HEECCCCCCCCCCCCCCEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHCCCEEEEEEHH
GKIYSTICEKKMLAGLIYGDSFPRNMHEKAHKMDFFDAKADVEALLTSLDLGGRYSFEKC
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCC
DGPSLYHPGKVSNVKIEGKEVGFIGELHPRVASNRQITIFELFLDKLACNLKLKENTFGK
CCCCCCCCCCCCEEEECCCEECEEECCCCHHCCCCCEEHHHHHHHHHHCCEEEECCCCCC
FPIVERDFSFVFDLDKVPLWGDIERGLYLISDFIKNVTLFDKYEGDFKGKRMLSLAFFVS
CCEEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCHHEEEEEEEEE
MENQTHGLSQEEIKSLSDQIICFVQGKFSGTLRLSC
ECCCCCCCCHHHHHHHHCEEEEEEECCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA