Click here to switch to the map view.

The map label for this gene is gatA

Identifier: 88607922

GI number: 88607922

Start: 794318

End: 795793

Strand: Direct

Name: gatA

Synonym: APH_0748

Alternate gene names: 88607922

Gene position: 794318-795793 (Clockwise)

Preceding gene: 88607757

Following gene: 88607652

Centisome position: 53.99

GC content: 42.75

Gene sequence:

>1476_bases
ATGAAGAAGGAACTATTAAAGCTTTCAATACTGGAAGCACATAATTGCCTCAAGAGAAAAGAATTTTCCCCTACTGAGCT
TGTAGAAGCGTACATAGATGAGATGGAGAATGAGAATCTCAATGCTTTTGTAACAAAAACGCAAGACATTGCATTAGAGC
AAGCAAGAAAGGTAGAAAACCTACTGCTTCAAGGTGAAAAGATAAGTCCATTAGCAGGTATGCCTATAGGAGTGAAAGAT
CTATTTTGCACCGCAGGAGTGCGCACTACCGCATGCTCTGACATATTAAAGAATTTCGTTCCTAGCTATGAGTCTACTAT
CTCTCAAAATCTCTGGAATAATAATGCTTGCATGATGGGGAAATTGAACATGGATGAATTTGCCATGGGCTCTGCTAGCA
GCTACAGCTGCTTCGGACCCGTGAAAAACCCATGGAAAGGCCCCTATGGCGAAGATCTTATACCTGGAGGGTCATCCGGG
GGATCTAGCGCAGCCGTTGCAGGTCGTCTATGCATAGCAGCCATTGGTAGTGATACTGGTGGGTCTGTAAGACAACCAGC
TGCCTTATGTGGCATAGTAGGCGCAAAACCCACTTATGGTCGCTGCTCTCGCTGGGGAATGATAGCTTTTGCAAGCTCGT
TAGATCAAGCAGGAGTGCTCACCCGCACTGTTGAAGATGCTGCTATAATGCTACAGGCAATGTGCGGACATGATAAAAAA
GATTCAACTTCTTCACCGGTGGACGTACCAGACTTCTTAAAACATATAAGTTACGATATCAAAGGAAAGCGCATAGGGAT
TGTAAGGGAGTATGCAATTCCTGAAAATAAGGAACGGGAATGTGTCTCAGAAATGTGGGCAGCAAACTTAAAGTACTTAC
AAGATTGTGGAGCTGAAATTGTAGACATAAGTTTGCATCATACCAAGTACGCGCTGCCTTTATACTACGTTATAGCATCA
TCTGAAGCTTCGTCTAATCTGGCTCGTTATGATGGCGTCAGGTATGGAGTTAGACGAGAAGGTGAAAGCATAGATGATAT
GTATGAGCTGACGAGAAGCTTGAATTTCGGAGAAGAAGTAAAACGTAGAATTCTTATCGGAGCATATGCTCTCTCCTATG
GGTGCTATGATGCATACTATGATAAATCACAGAGAGTTCGTCGCAGAGTTATGCAAGACTTCGATGAAGCCTTTAAAAAG
GTAGATTGCATACTGACTTTAACAACTCCGAGAAATTTCATTGGAATGGACGAAAAACTAGAAATGATAGACAGATACTA
TAGCGATGTCTTTACCGTACCTGCAAGCTTAGCAGGCCTTCCAGCCATCTCTATACCCTCTGGCCTAACGAAGCATGGCT
TGCCTATGTCATTACAAGTAATAGGTAAGCACTACGATGAAGGTGAAATTTTTAATGTAGCTGCGGTTATACACAAGCGT
ACTGGTGATCTTCTAAAGCACTTACACCACTTTTAG

Upstream 100 bases:

>100_bases
ACCTAGGCTTGAAGTCATATAACGTGACTAAATCGGTTGCCTTGCTCTCAAAGTGTGTTAACCTCCCATCACAATTGAGC
GTTTTTGTGGCCTGCTAAAT

Downstream 100 bases:

>100_bases
GTGCCTGACTACAGAAGAAGTAGCAACAGTCGCCATATATTTGTGTGTCGAGAGTTTATAGATTTGAGCAAAATTATTTG
CTAGTTATGTTATAGGGAAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MKKELLKLSILEAHNCLKRKEFSPTELVEAYIDEMENENLNAFVTKTQDIALEQARKVENLLLQGEKISPLAGMPIGVKD
LFCTAGVRTTACSDILKNFVPSYESTISQNLWNNNACMMGKLNMDEFAMGSASSYSCFGPVKNPWKGPYGEDLIPGGSSG
GSSAAVAGRLCIAAIGSDTGGSVRQPAALCGIVGAKPTYGRCSRWGMIAFASSLDQAGVLTRTVEDAAIMLQAMCGHDKK
DSTSSPVDVPDFLKHISYDIKGKRIGIVREYAIPENKERECVSEMWAANLKYLQDCGAEIVDISLHHTKYALPLYYVIAS
SEASSNLARYDGVRYGVRREGESIDDMYELTRSLNFGEEVKRRILIGAYALSYGCYDAYYDKSQRVRRRVMQDFDEAFKK
VDCILTLTTPRNFIGMDEKLEMIDRYYSDVFTVPASLAGLPAISIPSGLTKHGLPMSLQVIGKHYDEGEIFNVAAVIHKR
TGDLLKHLHHF

Sequences:

>Translated_491_residues
MKKELLKLSILEAHNCLKRKEFSPTELVEAYIDEMENENLNAFVTKTQDIALEQARKVENLLLQGEKISPLAGMPIGVKD
LFCTAGVRTTACSDILKNFVPSYESTISQNLWNNNACMMGKLNMDEFAMGSASSYSCFGPVKNPWKGPYGEDLIPGGSSG
GSSAAVAGRLCIAAIGSDTGGSVRQPAALCGIVGAKPTYGRCSRWGMIAFASSLDQAGVLTRTVEDAAIMLQAMCGHDKK
DSTSSPVDVPDFLKHISYDIKGKRIGIVREYAIPENKERECVSEMWAANLKYLQDCGAEIVDISLHHTKYALPLYYVIAS
SEASSNLARYDGVRYGVRREGESIDDMYELTRSLNFGEEVKRRILIGAYALSYGCYDAYYDKSQRVRRRVMQDFDEAFKK
VDCILTLTTPRNFIGMDEKLEMIDRYYSDVFTVPASLAGLPAISIPSGLTKHGLPMSLQVIGKHYDEGEIFNVAAVIHKR
TGDLLKHLHHF
>Mature_491_residues
MKKELLKLSILEAHNCLKRKEFSPTELVEAYIDEMENENLNAFVTKTQDIALEQARKVENLLLQGEKISPLAGMPIGVKD
LFCTAGVRTTACSDILKNFVPSYESTISQNLWNNNACMMGKLNMDEFAMGSASSYSCFGPVKNPWKGPYGEDLIPGGSSG
GSSAAVAGRLCIAAIGSDTGGSVRQPAALCGIVGAKPTYGRCSRWGMIAFASSLDQAGVLTRTVEDAAIMLQAMCGHDKK
DSTSSPVDVPDFLKHISYDIKGKRIGIVREYAIPENKERECVSEMWAANLKYLQDCGAEIVDISLHHTKYALPLYYVIAS
SEASSNLARYDGVRYGVRREGESIDDMYELTRSLNFGEEVKRRILIGAYALSYGCYDAYYDKSQRVRRRVMQDFDEAFKK
VDCILTLTTPRNFIGMDEKLEMIDRYYSDVFTVPASLAGLPAISIPSGLTKHGLPMSLQVIGKHYDEGEIFNVAAVIHKR
TGDLLKHLHHF

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=500, Percent_Identity=41, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI195972892, Length=498, Percent_Identity=23.2931726907631, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17543272, Length=449, Percent_Identity=37.8619153674833, Blast_Score=261, Evalue=7e-70,
Organism=Caenorhabditis elegans, GI17537465, Length=259, Percent_Identity=29.3436293436293, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17556264, Length=270, Percent_Identity=26.2962962962963, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71990152, Length=205, Percent_Identity=29.2682926829268, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=431, Percent_Identity=35.0348027842227, Blast_Score=229, Evalue=9e-61,
Organism=Saccharomyces cerevisiae, GI6319685, Length=436, Percent_Identity=28.4403669724771, Blast_Score=98, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24648113, Length=504, Percent_Identity=38.4920634920635, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24644968, Length=519, Percent_Identity=25.626204238921, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI161078093, Length=504, Percent_Identity=23.8095238095238, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI45550774, Length=510, Percent_Identity=21.5686274509804, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24648435, Length=510, Percent_Identity=21.5686274509804, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24648437, Length=510, Percent_Identity=21.5686274509804, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24652985, Length=509, Percent_Identity=24.5579567779961, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19922090, Length=509, Percent_Identity=24.5579567779961, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24652981, Length=509, Percent_Identity=24.5579567779961, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24652983, Length=509, Percent_Identity=24.5579567779961, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21356731, Length=507, Percent_Identity=24.0631163708087, Blast_Score=82, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_ANAPZ (Q2GJX4)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_505327.1
- ProteinModelPortal:   Q2GJX4
- SMR:   Q2GJX4
- STRING:   Q2GJX4
- GeneID:   3930758
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0748
- NMPDR:   fig|212042.5.peg.728
- TIGR:   APH_0748
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- PhylomeDB:   Q2GJX4
- ProtClustDB:   PRK00012
- BioCyc:   APHA212042:APH_0748-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 54269; Mature: 54269

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 79-79 ACT_SITE 158-158 ACT_SITE 182-182

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKELLKLSILEAHNCLKRKEFSPTELVEAYIDEMENENLNAFVTKTQDIALEQARKVEN
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHH
LLLQGEKISPLAGMPIGVKDLFCTAGVRTTACSDILKNFVPSYESTISQNLWNNNACMMG
HHHCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEE
KLNMDEFAMGSASSYSCFGPVKNPWKGPYGEDLIPGGSSGGSSAAVAGRLCIAAIGSDTG
CCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEEECCCCC
GSVRQPAALCGIVGAKPTYGRCSRWGMIAFASSLDQAGVLTRTVEDAAIMLQAMCGHDKK
CCCCCCHHHHHHCCCCCCCCCHHHCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCC
DSTSSPVDVPDFLKHISYDIKGKRIGIVREYAIPENKERECVSEMWAANLKYLQDCGAEI
CCCCCCCCHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCE
VDISLHHTKYALPLYYVIASSEASSNLARYDGVRYGVRREGESIDDMYELTRSLNFGEEV
EEEEECCCEEECEEEEEEECCCCCCCHHHHCCHHHCHHCCCCCHHHHHHHHHHCCCHHHH
KRRILIGAYALSYGCYDAYYDKSQRVRRRVMQDFDEAFKKVDCILTLTTPRNFIGMDEKL
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCHHCCCHHHH
EMIDRYYSDVFTVPASLAGLPAISIPSGLTKHGLPMSLQVIGKHYDEGEIFNVAAVIHKR
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCEEHHHHHCCCCCCCHHHHHHHHHHH
TGDLLKHLHHF
HHHHHHHHHCC
>Mature Secondary Structure
MKKELLKLSILEAHNCLKRKEFSPTELVEAYIDEMENENLNAFVTKTQDIALEQARKVEN
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHH
LLLQGEKISPLAGMPIGVKDLFCTAGVRTTACSDILKNFVPSYESTISQNLWNNNACMMG
HHHCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEE
KLNMDEFAMGSASSYSCFGPVKNPWKGPYGEDLIPGGSSGGSSAAVAGRLCIAAIGSDTG
CCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEEECCCCC
GSVRQPAALCGIVGAKPTYGRCSRWGMIAFASSLDQAGVLTRTVEDAAIMLQAMCGHDKK
CCCCCCHHHHHHCCCCCCCCCHHHCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCC
DSTSSPVDVPDFLKHISYDIKGKRIGIVREYAIPENKERECVSEMWAANLKYLQDCGAEI
CCCCCCCCHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCE
VDISLHHTKYALPLYYVIASSEASSNLARYDGVRYGVRREGESIDDMYELTRSLNFGEEV
EEEEECCCEEECEEEEEEECCCCCCCHHHHCCHHHCHHCCCCCHHHHHHHHHHCCCHHHH
KRRILIGAYALSYGCYDAYYDKSQRVRRRVMQDFDEAFKKVDCILTLTTPRNFIGMDEKL
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCHHCCCHHHH
EMIDRYYSDVFTVPASLAGLPAISIPSGLTKHGLPMSLQVIGKHYDEGEIFNVAAVIHKR
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCEEHHHHHCCCCCCCHHHHHHHHHHH
TGDLLKHLHHF
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA