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The map label for this gene is proP [H]

Identifier: 88607830

GI number: 88607830

Start: 62305

End: 63588

Strand: Reverse

Name: proP [H]

Synonym: APH_0053

Alternate gene names: 88607830

Gene position: 63588-62305 (Counterclockwise)

Preceding gene: 88606715

Following gene: 88606817

Centisome position: 4.32

GC content: 44.31

Gene sequence:

>1284_bases
ATGAAGAAGGGAACAGTAAAACGCGCCGTGTTGTCTACTATCCTATGTAATACCTTAGTATGGTATGATTATATACTTTT
TGGAAGCTTAGCCAGTACTATCGGCGACCTATTCTTCCCTCAAGAAGACAAATACTTGTCCTTGATGAGCAGTTTCTCTG
TTTTTGCTGTTGGTTTTTGCATGCGCCCATTCGGTGCGAGCATATTTGGACATATCGGTGATAAATATGGTAGGAGGACA
GCGCTCGTCATATCCATCATTGCGATGTCAGTGCCTATAGGATTCATGGCTGTAGTGCCGACCTATGCTAGTGTGGGGGT
ACTTGCCCCTATCTTACTCGTAATATGTAGATTGGTACAAGGTTTCTCCCTGGGAGGTGAGGCAGGAAATGCTACTTTCC
TTATAGAACACTCAAAAGCAAATCAAGCTGGGCTCTTCGGGAGTTTCGAGGTTCTGAGTGCGGTTTTGGGATCTGTGTTT
GCGTTAGCCGTTAAGATGGCATCATTTTATTTCACAGGCGACGAGTTCAAGGTCTGGGGATGGAGAATCCCGTTCATAGT
GGGATTTGTTATTGGTATCATCAGCATATTCATAAGGGTTAGGGCCGGCGAAAGCCCTGCGTATACAGAGCATGCGTCTA
AAGAGAGTGGCGTAGTGAAGTCACCTGTAAAACATCTGTTTAAGTACTACAGAAGGCCTTTGATACTAGCGATTTGCATA
GACTGTATAGAGAACTGCTCTTTCCACATTTTTATGGTTTTCTTCATAAGCTTTGTAGGTAACTTTTCTCTATCATCTGC
AGTAAATCCGCAAATTGGCGAATTTATAGAAATAGTAAACGTGATGATCAGTGGGGTGATGACTGTAGGGTTCGGTGCAT
TATCTGACGTGATTGGTCGAAAGAAGGTGATGGGTGGCGCATCTATTGCGCTCTTCTTCCTTGCTATACCTGTGTTCTGG
CTATTGAGTCAGGAAAGTTTCTGGTATGTAGGGTTGGGTTACTTTCTGTTTGCGATTCCGTTTGCTGCTACACTTGGTCC
ATCCAGTGCTGCGATGTCTGAGCTATTCCCCACAAAAGTGCGTTATACTGGCTTTGGAATAGCCCGTAACATATCTTCAG
CTCTTGGTGGAGGTATGGCTCCTGTGCTTTGTACATGGCTTATGCAAATTACAGGTTGGAAGGTTGCACCTGGTTTTTGT
GTGATGTTCTGGGCGTTGATTGCATGTATTGCATTGAGTAGGATTACCCCAAAGGACATTCATAGAGACTGGGTCAAACA
GTAG

Upstream 100 bases:

>100_bases
CGTGTTTGTGAGCACCTAATTTGTCATGGAATGGGGGTGGCACTGTGGAAAAGCCTGAGTGTTATTAGCATTTAGCGTGA
CTACATAAGTTGGAGGTTTT

Downstream 100 bases:

>100_bases
TACTCTACTGCTGGGGTTTCTTACTAAAGTCTAGCAGCCATTGTTAATGGCTGCTTGTTAGTTTCCTGAGTAATAAGTTA
TTGATTGTCCGATGATTGGA

Product: major facilitator family transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MKKGTVKRAVLSTILCNTLVWYDYILFGSLASTIGDLFFPQEDKYLSLMSSFSVFAVGFCMRPFGASIFGHIGDKYGRRT
ALVISIIAMSVPIGFMAVVPTYASVGVLAPILLVICRLVQGFSLGGEAGNATFLIEHSKANQAGLFGSFEVLSAVLGSVF
ALAVKMASFYFTGDEFKVWGWRIPFIVGFVIGIISIFIRVRAGESPAYTEHASKESGVVKSPVKHLFKYYRRPLILAICI
DCIENCSFHIFMVFFISFVGNFSLSSAVNPQIGEFIEIVNVMISGVMTVGFGALSDVIGRKKVMGGASIALFFLAIPVFW
LLSQESFWYVGLGYFLFAIPFAATLGPSSAAMSELFPTKVRYTGFGIARNISSALGGGMAPVLCTWLMQITGWKVAPGFC
VMFWALIACIALSRITPKDIHRDWVKQ

Sequences:

>Translated_427_residues
MKKGTVKRAVLSTILCNTLVWYDYILFGSLASTIGDLFFPQEDKYLSLMSSFSVFAVGFCMRPFGASIFGHIGDKYGRRT
ALVISIIAMSVPIGFMAVVPTYASVGVLAPILLVICRLVQGFSLGGEAGNATFLIEHSKANQAGLFGSFEVLSAVLGSVF
ALAVKMASFYFTGDEFKVWGWRIPFIVGFVIGIISIFIRVRAGESPAYTEHASKESGVVKSPVKHLFKYYRRPLILAICI
DCIENCSFHIFMVFFISFVGNFSLSSAVNPQIGEFIEIVNVMISGVMTVGFGALSDVIGRKKVMGGASIALFFLAIPVFW
LLSQESFWYVGLGYFLFAIPFAATLGPSSAAMSELFPTKVRYTGFGIARNISSALGGGMAPVLCTWLMQITGWKVAPGFC
VMFWALIACIALSRITPKDIHRDWVKQ
>Mature_427_residues
MKKGTVKRAVLSTILCNTLVWYDYILFGSLASTIGDLFFPQEDKYLSLMSSFSVFAVGFCMRPFGASIFGHIGDKYGRRT
ALVISIIAMSVPIGFMAVVPTYASVGVLAPILLVICRLVQGFSLGGEAGNATFLIEHSKANQAGLFGSFEVLSAVLGSVF
ALAVKMASFYFTGDEFKVWGWRIPFIVGFVIGIISIFIRVRAGESPAYTEHASKESGVVKSPVKHLFKYYRRPLILAICI
DCIENCSFHIFMVFFISFVGNFSLSSAVNPQIGEFIEIVNVMISGVMTVGFGALSDVIGRKKVMGGASIALFFLAIPVFW
LLSQESFWYVGLGYFLFAIPFAATLGPSSAAMSELFPTKVRYTGFGIARNISSALGGGMAPVLCTWLMQITGWKVAPGFC
VMFWALIACIALSRITPKDIHRDWVKQ

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=422, Percent_Identity=30.5687203791469, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI1789941, Length=398, Percent_Identity=31.9095477386935, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI1788292, Length=426, Percent_Identity=26.9953051643192, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1788942, Length=379, Percent_Identity=28.4960422163588, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI87082404, Length=343, Percent_Identity=26.2390670553936, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI87082231, Length=428, Percent_Identity=24.2990654205607, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46598; Mature: 46598

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKGTVKRAVLSTILCNTLVWYDYILFGSLASTIGDLFFPQEDKYLSLMSSFSVFAVGFC
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
MRPFGASIFGHIGDKYGRRTALVISIIAMSVPIGFMAVVPTYASVGVLAPILLVICRLVQ
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFSLGGEAGNATFLIEHSKANQAGLFGSFEVLSAVLGSVFALAVKMASFYFTGDEFKVWG
CCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEC
WRIPFIVGFVIGIISIFIRVRAGESPAYTEHASKESGVVKSPVKHLFKYYRRPLILAICI
CHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DCIENCSFHIFMVFFISFVGNFSLSSAVNPQIGEFIEIVNVMISGVMTVGFGALSDVIGR
HHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKVMGGASIALFFLAIPVFWLLSQESFWYVGLGYFLFAIPFAATLGPSSAAMSELFPTKV
HHHCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHH
RYTGFGIARNISSALGGGMAPVLCTWLMQITGWKVAPGFCVMFWALIACIALSRITPKDI
EECCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHH
HRDWVKQ
HHHHHCC
>Mature Secondary Structure
MKKGTVKRAVLSTILCNTLVWYDYILFGSLASTIGDLFFPQEDKYLSLMSSFSVFAVGFC
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
MRPFGASIFGHIGDKYGRRTALVISIIAMSVPIGFMAVVPTYASVGVLAPILLVICRLVQ
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFSLGGEAGNATFLIEHSKANQAGLFGSFEVLSAVLGSVFALAVKMASFYFTGDEFKVWG
CCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEC
WRIPFIVGFVIGIISIFIRVRAGESPAYTEHASKESGVVKSPVKHLFKYYRRPLILAICI
CHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DCIENCSFHIFMVFFISFVGNFSLSSAVNPQIGEFIEIVNVMISGVMTVGFGALSDVIGR
HHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKVMGGASIALFFLAIPVFWLLSQESFWYVGLGYFLFAIPFAATLGPSSAAMSELFPTKV
HHHCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHH
RYTGFGIARNISSALGGGMAPVLCTWLMQITGWKVAPGFCVMFWALIACIALSRITPKDI
EECCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHH
HRDWVKQ
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]