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The map label for this gene is truB

Identifier: 88607808

GI number: 88607808

Start: 816699

End: 817601

Strand: Reverse

Name: truB

Synonym: APH_0769

Alternate gene names: 88607808

Gene position: 817601-816699 (Counterclockwise)

Preceding gene: 88606782

Following gene: 88606780

Centisome position: 55.57

GC content: 44.41

Gene sequence:

>903_bases
ATGAAGCATGGATGGTTAAATATAGACAAACCATATGGGGTGAGCTCAGGTAGCGTTGTAGGCAAGTTAAAGAAGATGCT
GCAGTGCAAAGTGGGGCATGCAGGAACATTGGATCCTCTTGCAACGGGCGTGCTTCCCATAGCGTTTGGTGAAGCAACAA
AGACCACTAATTACGCGACTGACACCTTGAAGTCCTATGAAGTTACAATTCAGTGGGGTGAGCAGCGTGACACTGATGAT
AAAGAAGGCAAGATTATCAGGACTAGTACCCTTAGACCGACTGAGCATAGTATTAAAGAGGCGTTGCAACAATATGTTGG
AGTTATACAACAAATACCATCGACTTTTTCTGCTATAAAGGTTGGAGGTATGCGTGCATATAGCTTATCACGAATGGGAA
AAGAGGTAGCTTTAGCCCCGAGGAGCGTGTGCATTACGGAAATAGAATTACTGAGTGTGGATTCTGGTAGTAACACAGCT
GATCTTAGAATTACTTGTGGGAAAGGGGTTTATGTCCGTGCTGTTGCGCGGGATTTAGGCATTACTCTTGGGTGTTATGG
ATATGTTGCGAGATTGCGTCGTACGATGGTTGGTCCCTTTACAGAAGCTAATATCGTAACCTTGCAGGAGCTAGAGACGT
TAGTAGGTGAAAATAAGCTAGAGGAAGCGGTATTTCCACTTAGCATTGTTATGTCTGGTTTGCCACATATAGAAATAGAT
GTAGAAACGGCCAAAGTAGTAAAAAATGGCCGCAATATAAGATTGGTAGATGCTGCTTTAAATGGCTTGTACATCGTAGA
GAATTGTGATATGTGTTACCTAAGCCAGGCGGGTGGCGTTCCTGTCGCTATCTGTGAAGTCGTAGATGGCACCGCGAAGC
CGGTGCGGGTTTTTAATGTCTGA

Upstream 100 bases:

>100_bases
GGAGTTTGTACAGTCTATATTGATAAACGCATTTGGCATAGGGGTTTTTCGTGATATTACTTCAGGAGTGAAATATAAAA
GCTTTATATCGGCAATAGCC

Downstream 100 bases:

>100_bases
GTTTTTAATATAGTATGGGGGTGCTTGCGTTTTTATGTCGATTACACCTAGTAAAAAGTCTGAGTTGATTTCTGAGTATC
GTATTAAGGAAGGGGATACC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MKHGWLNIDKPYGVSSGSVVGKLKKMLQCKVGHAGTLDPLATGVLPIAFGEATKTTNYATDTLKSYEVTIQWGEQRDTDD
KEGKIIRTSTLRPTEHSIKEALQQYVGVIQQIPSTFSAIKVGGMRAYSLSRMGKEVALAPRSVCITEIELLSVDSGSNTA
DLRITCGKGVYVRAVARDLGITLGCYGYVARLRRTMVGPFTEANIVTLQELETLVGENKLEEAVFPLSIVMSGLPHIEID
VETAKVVKNGRNIRLVDAALNGLYIVENCDMCYLSQAGGVPVAICEVVDGTAKPVRVFNV

Sequences:

>Translated_300_residues
MKHGWLNIDKPYGVSSGSVVGKLKKMLQCKVGHAGTLDPLATGVLPIAFGEATKTTNYATDTLKSYEVTIQWGEQRDTDD
KEGKIIRTSTLRPTEHSIKEALQQYVGVIQQIPSTFSAIKVGGMRAYSLSRMGKEVALAPRSVCITEIELLSVDSGSNTA
DLRITCGKGVYVRAVARDLGITLGCYGYVARLRRTMVGPFTEANIVTLQELETLVGENKLEEAVFPLSIVMSGLPHIEID
VETAKVVKNGRNIRLVDAALNGLYIVENCDMCYLSQAGGVPVAICEVVDGTAKPVRVFNV
>Mature_300_residues
MKHGWLNIDKPYGVSSGSVVGKLKKMLQCKVGHAGTLDPLATGVLPIAFGEATKTTNYATDTLKSYEVTIQWGEQRDTDD
KEGKIIRTSTLRPTEHSIKEALQQYVGVIQQIPSTFSAIKVGGMRAYSLSRMGKEVALAPRSVCITEIELLSVDSGSNTA
DLRITCGKGVYVRAVARDLGITLGCYGYVARLRRTMVGPFTEANIVTLQELETLVGENKLEEAVFPLSIVMSGLPHIEID
VETAKVVKNGRNIRLVDAALNGLYIVENCDMCYLSQAGGVPVAICEVVDGTAKPVRVFNV

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=217, Percent_Identity=31.3364055299539, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI2367200, Length=271, Percent_Identity=36.5313653136531, Blast_Score=168, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17553978, Length=238, Percent_Identity=28.1512605042017, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324037, Length=156, Percent_Identity=35.2564102564103, Blast_Score=82, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6323204, Length=243, Percent_Identity=27.5720164609054, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281364189, Length=236, Percent_Identity=26.6949152542373, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281364187, Length=236, Percent_Identity=26.6949152542373, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281364185, Length=236, Percent_Identity=26.6949152542373, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281364183, Length=236, Percent_Identity=26.6949152542373, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI62471759, Length=236, Percent_Identity=26.6949152542373, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17975520, Length=236, Percent_Identity=26.6949152542373, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_ANAPZ (Q2GJV3)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_505348.1
- ProteinModelPortal:   Q2GJV3
- SMR:   Q2GJV3
- STRING:   Q2GJV3
- GeneID:   3930851
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0769
- NMPDR:   fig|212042.5.peg.744
- TIGR:   APH_0769
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- PhylomeDB:   Q2GJV3
- ProtClustDB:   CLSK2516851
- BioCyc:   APHA212042:APH_0769-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 32466; Mature: 32466

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: NA

Important sites: ACT_SITE 38-38

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHGWLNIDKPYGVSSGSVVGKLKKMLQCKVGHAGTLDPLATGVLPIAFGEATKTTNYAT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCEEEEECCCCCCCCCHH
DTLKSYEVTIQWGEQRDTDDKEGKIIRTSTLRPTEHSIKEALQQYVGVIQQIPSTFSAIK
HCCEEEEEEEEECCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHE
VGGMRAYSLSRMGKEVALAPRSVCITEIELLSVDSGSNTADLRITCGKGVYVRAVARDLG
ECCEEEHHHHHCCCHHCCCCCCEEEEEEEEEEECCCCCCEEEEEEECCCEEEEEEHHHHC
ITLGCYGYVARLRRTMVGPFTEANIVTLQELETLVGENKLEEAVFPLSIVMSGLPHIEID
CCHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEE
VETAKVVKNGRNIRLVDAALNGLYIVENCDMCYLSQAGGVPVAICEVVDGTAKPVRVFNV
CHHHHHHHCCCCEEEEEECCCCEEEEECCCEEEEECCCCCCEEEEEECCCCCCCEEEECC
>Mature Secondary Structure
MKHGWLNIDKPYGVSSGSVVGKLKKMLQCKVGHAGTLDPLATGVLPIAFGEATKTTNYAT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCEEEEECCCCCCCCCHH
DTLKSYEVTIQWGEQRDTDDKEGKIIRTSTLRPTEHSIKEALQQYVGVIQQIPSTFSAIK
HCCEEEEEEEEECCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHE
VGGMRAYSLSRMGKEVALAPRSVCITEIELLSVDSGSNTADLRITCGKGVYVRAVARDLG
ECCEEEHHHHHCCCHHCCCCCCEEEEEEEEEEECCCCCCEEEEEEECCCEEEEEEHHHHC
ITLGCYGYVARLRRTMVGPFTEANIVTLQELETLVGENKLEEAVFPLSIVMSGLPHIEID
CCHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEE
VETAKVVKNGRNIRLVDAALNGLYIVENCDMCYLSQAGGVPVAICEVVDGTAKPVRVFNV
CHHHHHHHCCCCEEEEEECCCCEEEEECCCEEEEECCCCCCEEEEEECCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA