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The map label for this gene is rsmE [H]

Identifier: 88607769

GI number: 88607769

Start: 838232

End: 838951

Strand: Reverse

Name: rsmE [H]

Synonym: APH_0794

Alternate gene names: 88607769

Gene position: 838951-838232 (Counterclockwise)

Preceding gene: 88607185

Following gene: 88607608

Centisome position: 57.02

GC content: 43.19

Gene sequence:

>720_bases
ATGGTTTTCAAAAAGAAGCCCGTAAAAATCAGGCTATATTACAGCGATAGCGCACTGAATAAGGGTGAAAAGGTCCTCTT
GCGTTCTGATCAAGCTCATTACATAAGAGACGTGATGAGAGTGAGGATATCAGATGCTGTTGTACTCTTTGATGGTGTAA
CAGGAGATTGGTTCGCTACTATTTCAGAAATATCTCGTAAGAAAGTAGAGGTGCGGGTTGAACACCTGGTTAGTGAATAT
GTAAAGAGCAGGGATCTTATGCTTTGCATTGCGCTTGTAAAGCAGGATACCATGCGTAGTGTTGTGCGACAAGCAACAGA
GATGGGAGTAACGCAAATTCAGCCCATTCGTACGGAATATTCTGTAGTTAGTGATATAAATGTGCAAAAGTGTCGCCAAT
GGGCAATAGAGGCTGCTGAACAGTGTGAAAGGCAAGATATTCCGGAGATTGCATCGGTCATGGAATTTACGCATCTCAAT
GACCTTGGTAAGAAATTGGTACTATGTGATGAAACGGGTAAAGGAAGAATGCCTGCTGAGGTGCTGCGTAGTAAGGAGAA
CTTGGGCATCATAATTGGTCCAGAAGGGGGTTTTTCCGAGAGAGAGCTTGTCTGTGCAGAGAGTTTTTGTGAAAAGATGT
CTTTGGGGCATAGGATTCTAAGAGTTGATACGGCAGTTGTGGCAGCTCTTGCATACGTGAATGAGTACTATATCTCATAG

Upstream 100 bases:

>100_bases
CAAGAGGATATGGAGGAATGTTAAAGTACATGATCATGTTGATACTGTATTAAAAGCGGTAAATGAGCTTTAGTAGTTTC
TAGAAAAGCAATTATACTTT

Downstream 100 bases:

>100_bases
GTATTATTTCAGAGGTACTATCTACTGTATAATGAGCTGTTGTTGTGCTTATAAAGCGTCTAACAGCATGCGTAATTTAG
TATACAGTACTTCATAAGCA

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MVFKKKPVKIRLYYSDSALNKGEKVLLRSDQAHYIRDVMRVRISDAVVLFDGVTGDWFATISEISRKKVEVRVEHLVSEY
VKSRDLMLCIALVKQDTMRSVVRQATEMGVTQIQPIRTEYSVVSDINVQKCRQWAIEAAEQCERQDIPEIASVMEFTHLN
DLGKKLVLCDETGKGRMPAEVLRSKENLGIIIGPEGGFSERELVCAESFCEKMSLGHRILRVDTAVVAALAYVNEYYIS

Sequences:

>Translated_239_residues
MVFKKKPVKIRLYYSDSALNKGEKVLLRSDQAHYIRDVMRVRISDAVVLFDGVTGDWFATISEISRKKVEVRVEHLVSEY
VKSRDLMLCIALVKQDTMRSVVRQATEMGVTQIQPIRTEYSVVSDINVQKCRQWAIEAAEQCERQDIPEIASVMEFTHLN
DLGKKLVLCDETGKGRMPAEVLRSKENLGIIIGPEGGFSERELVCAESFCEKMSLGHRILRVDTAVVAALAYVNEYYIS
>Mature_239_residues
MVFKKKPVKIRLYYSDSALNKGEKVLLRSDQAHYIRDVMRVRISDAVVLFDGVTGDWFATISEISRKKVEVRVEHLVSEY
VKSRDLMLCIALVKQDTMRSVVRQATEMGVTQIQPIRTEYSVVSDINVQKCRQWAIEAAEQCERQDIPEIASVMEFTHLN
DLGKKLVLCDETGKGRMPAEVLRSKENLGIIIGPEGGFSERELVCAESFCEKMSLGHRILRVDTAVVAALAYVNEYYIS

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=240, Percent_Identity=29.1666666666667, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27137; Mature: 27137

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFKKKPVKIRLYYSDSALNKGEKVLLRSDQAHYIRDVMRVRISDAVVLFDGVTGDWFAT
CCCCCCCEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHCCEEEEEECCCCHHHHH
ISEISRKKVEVRVEHLVSEYVKSRDLMLCIALVKQDTMRSVVRQATEMGVTQIQPIRTEY
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCHHHHHH
SVVSDINVQKCRQWAIEAAEQCERQDIPEIASVMEFTHLNDLGKKLVLCDETGKGRMPAE
HHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHH
VLRSKENLGIIIGPEGGFSERELVCAESFCEKMSLGHRILRVDTAVVAALAYVNEYYIS
HHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVFKKKPVKIRLYYSDSALNKGEKVLLRSDQAHYIRDVMRVRISDAVVLFDGVTGDWFAT
CCCCCCCEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHCCEEEEEECCCCHHHHH
ISEISRKKVEVRVEHLVSEYVKSRDLMLCIALVKQDTMRSVVRQATEMGVTQIQPIRTEY
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCHHHHHH
SVVSDINVQKCRQWAIEAAEQCERQDIPEIASVMEFTHLNDLGKKLVLCDETGKGRMPAE
HHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHH
VLRSKENLGIIIGPEGGFSERELVCAESFCEKMSLGHRILRVDTAVVAALAYVNEYYIS
HHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]