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The map label for this gene is thiE [H]

Identifier: 88607680

GI number: 88607680

Start: 1022330

End: 1022932

Strand: Reverse

Name: thiE [H]

Synonym: APH_0960

Alternate gene names: 88607680

Gene position: 1022932-1022330 (Counterclockwise)

Preceding gene: 88607313

Following gene: 88606991

Centisome position: 69.53

GC content: 42.62

Gene sequence:

>603_bases
ATGGATGATATTGGTTTTTACCTAATAGTTCCTGATGCACATTGGTTTGAATTTGCTGCGAGCTGTGGTGTAAGAACTAT
ACAGCTTAGGGCAAAGGATAAGCCTGTAAGCGAAGTGGAAAACATGGTGAAGCGTTGTTCTGAAATTGCAGTACGCTACG
GTGTAAGATTTATAGTGAATGATTATTGGGAGCTAGCGCTAAAATACAATGCATACGGAATACATCTTGGGCAAGAAGAT
ATAAAAACTGCTGACCTGTATAGGATATCATCCTCTGGAATACGACTAGGAATTAGTACACACTGTTACCATGAGTTTGC
ACGTGCAAGCTTCTATAAGCCATCATATATTGCACTTGGACCTATATATGACACCACATCTAAGAGCATGCAGTTTCATG
CGCAGGGGTTGGAGCTTCTAAAGCAATGGGTGAAGAGCAGTTCTTGTCCTGTGGTGGCTATTGGCGGCATTACGCTAGCT
AACATAGATGATGTTGTGCGGTGCAACGCTGGTGGAGTAGCTGTTATCTCTGCTGTGACTGAGGCTGCTGATCCAAAAGA
TGCTATACGTCGATTTCTCGATAAATGTAGTGTGGGTGTATAA

Upstream 100 bases:

>100_bases
TGGTCTAACACCGCTAGATAGTAGAAGTGCAGATATACGAGAGCATTGCCCGCATATATCTTCTAGTAAGGATGAAATCG
GTGAGTTTTGCTTTCAAGGT

Downstream 100 bases:

>100_bases
GTAAGGAAAAGGCAGCATTATCTTTATTTAACAGCCAACATCTTATCAGATAGAAGGGTTGAAAGGACCTTTAGCAAAAA
TTGCATGTTTTCAAATTATA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MDDIGFYLIVPDAHWFEFAASCGVRTIQLRAKDKPVSEVENMVKRCSEIAVRYGVRFIVNDYWELALKYNAYGIHLGQED
IKTADLYRISSSGIRLGISTHCYHEFARASFYKPSYIALGPIYDTTSKSMQFHAQGLELLKQWVKSSSCPVVAIGGITLA
NIDDVVRCNAGGVAVISAVTEAADPKDAIRRFLDKCSVGV

Sequences:

>Translated_200_residues
MDDIGFYLIVPDAHWFEFAASCGVRTIQLRAKDKPVSEVENMVKRCSEIAVRYGVRFIVNDYWELALKYNAYGIHLGQED
IKTADLYRISSSGIRLGISTHCYHEFARASFYKPSYIALGPIYDTTSKSMQFHAQGLELLKQWVKSSSCPVVAIGGITLA
NIDDVVRCNAGGVAVISAVTEAADPKDAIRRFLDKCSVGV
>Mature_200_residues
MDDIGFYLIVPDAHWFEFAASCGVRTIQLRAKDKPVSEVENMVKRCSEIAVRYGVRFIVNDYWELALKYNAYGIHLGQED
IKTADLYRISSSGIRLGISTHCYHEFARASFYKPSYIALGPIYDTTSKSMQFHAQGLELLKQWVKSSSCPVVAIGGITLA
NIDDVVRCNAGGVAVISAVTEAADPKDAIRRFLDKCSVGV

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=196, Percent_Identity=44.8979591836735, Blast_Score=167, Evalue=5e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 22165; Mature: 22165

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDIGFYLIVPDAHWFEFAASCGVRTIQLRAKDKPVSEVENMVKRCSEIAVRYGVRFIVN
CCCCEEEEEECCCHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DYWELALKYNAYGIHLGQEDIKTADLYRISSSGIRLGISTHCYHEFARASFYKPSYIALG
HHHHEEEEECEEEEEECHHHCCHHHEEEECCCCEEEECHHHHHHHHHHHCCCCCCEEEEC
PIYDTTSKSMQFHAQGLELLKQWVKSSSCPVVAIGGITLANIDDVVRCNAGGVAVISAVT
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCEEEECCCHHEEECCCCCHHHHHHH
EAADPKDAIRRFLDKCSVGV
HCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDDIGFYLIVPDAHWFEFAASCGVRTIQLRAKDKPVSEVENMVKRCSEIAVRYGVRFIVN
CCCCEEEEEECCCHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DYWELALKYNAYGIHLGQEDIKTADLYRISSSGIRLGISTHCYHEFARASFYKPSYIALG
HHHHEEEEECEEEEEECHHHCCHHHEEEECCCCEEEECHHHHHHHHHHHCCCCCCEEEEC
PIYDTTSKSMQFHAQGLELLKQWVKSSSCPVVAIGGITLANIDDVVRCNAGGVAVISAVT
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCEEEECCCHHEEECCCCCHHHHHHH
EAADPKDAIRRFLDKCSVGV
HCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]