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The map label for this gene is topA [H]

Identifier: 88607358

GI number: 88607358

Start: 620600

End: 623035

Strand: Direct

Name: topA [H]

Synonym: APH_0597

Alternate gene names: 88607358

Gene position: 620600-623035 (Clockwise)

Preceding gene: 88607136

Following gene: 88607438

Centisome position: 42.18

GC content: 41.75

Gene sequence:

>2436_bases
ATGGGCGTGGTCATAGTAGAGTCTCCTTCTAAGGCGAAGACTATTAGTAAGTACTTGGGGAAGAAATTCAATGTTATTGC
TTCTTTTGGCCACATAAGGGATTTTCCAGCGAAGAGTGGATCAGTTGATCCTTCTAATGATTTTAAAATGATATATGAGA
TTATCCCAAAATCAGAAAAACACCTGGATAAGATAGTAAAAACTGTCAGTGCTGAAAAAGAGGCTATATATCTCGCTACG
GATCCGGATCGCGAAGGAGAGGCGATAGCTTGGCATATAGTAGAGGTTTTGAAGGAGAAGTGTGCCATAGCGAAAGATGT
TACAGTGAACAGGATGGTGTTCAATGAAGTAACCAAAAGAGCAGTTACTGCTGCTGTTGATAATTGCCGTCCTATAAATA
TGGACTTGGTTCGTGCGCAGCAAACACGCAGAGTGTTGGATTATCTCGTGGGGTTTACGCTGTCCCCGCTATTATGGAGA
AAGTTACCAGGCAGTAGGTCAGCAGGTAGAGTGCAATCTGTGGCGCTCCGTCTACTTTGTGAAAGAGAATATGAAGTAGA
GCAGTTCAAGACTCAGGAATATTGGGATGTTGGAGTAGATCTGCAAAACCAGGGTGGTGAGAAATTCGAGGCCGTTCTTA
AGTGTTATGCGGGTGAGAAGCTCGAGAAGTTTTCAATAGGCACAGAAGAGCAAGCCAAGGAATACGCAGAGACTGTTAAG
AATCAGAGCTATACTGTAGAATCTGTTGAGTCGAAAAAGACTAAGCGCAACCCATATCCTCCTTTTATCACATCGAGTCT
ACAACAAGAGGCATCTACGAAATTGGGGTTCTCTGCGAAGAATACCATGATGATAGCTCAGAAGTTGTATGAGGGTATAG
ATATCGGAGGGGAAATAGTAGGTCTTATAACGTACATGCGTACAGATGGTTTTTACATTTCTGAAGATGCCGTAAACTAT
ATACGTGATACAATCAAAATGCAGTTTGGGGAGAAGTATTTATCCAAATCTGTTAGGAATTACACTAAAAAAGTAAAGAA
TGCACAGGAAGCGCACGAAGCTATCCGTCCAACAGACATTACTATGCTACCTGAGCAGCTATCAAAATACCTCACGGATG
AGCAATTGAAGCTTTATACTCTCATATGGAAAAGGACACTTGCTACACAGATGGAGTCTGCAGTAATAGATCAGGTGGCT
GTGGATATTAAGTCATCGTGTGGGAAAATAAGTCTTCATGCAACTGGATCGACGCTCAGTTTTGATGGTTATTATAGAGT
ATATGGTTCTGAGGATAGTGATAAGCGTCGCATGTTGCCACAACTTCGCGAAGGTGAGAGCTGCGAGCTGTTGGAAGTAC
ATCCTAATCAGCATTTTACTCAGCCGCCTGCGCGATACAGCGAAGCTAGTTTAGTGAAAAAAATGGAAGAGATCGGCATT
GGTAGACCTTCTACTTATGCGACGATTATTTCAGTTTTGCAGGAAAGGGGGTACACTCAACTAGAGCAAAAGCGTTTTAT
TCCAAGTGAGCGGGGGAGAATAGTAAACGCCTTTTTGACGAACTTTTTTAGAAAGTACGTGGAGTATGACTTTACGGCTA
ATCTGGAGGAAGAGCTTGACCTTATCTCAAATGGAAAGATGGTTTGGAAGGAGGTTTTGAGTCAATTTTGGGATAAGTTT
ATATCGGATGTCAGTTCCGTAAAAACTATTGAAGCTAGTGAGATATTGAAAGCTATTACCAGAGATTTGGAGAATTATGC
ATTTTCTTCAGTAGATGGCGAAAGCATTAGCAGAGTTTGTCCACAGTGTGGATCGGGTGAGCTCATGCTCAATATCGGTA
AGACTAGTGCGTTTTTGGGATGCAATAGATACCCTGAATGTAGATATACGAGAGGAATTGGTGGAGATGGTAGCGCTTCT
CCGGCTGATGATGCCTCAAGAATTTTGGGTATAGATGAGGAGACTCAGGAAGAGATTTTCCTAAAGCGTGGGCCATACGG
TGAATATTTGCAGTTAGGTAACAGCACAAAGCGTGTTTCTTTAACTAAGAGCGCTGGTGAAATTACTGTAGATCTTGCGA
AGAAGATGTTATCTCTTCCATTGGTTCTTGGATGCTATCCGGATACTGATAAAGAGATCAAGTTGGGTATTGGCCGTTTC
GGACCATACCTTTTTTACAATGGCGTGTATTTTTCTATCAAAGATAGGGAAGACTACATCAACATAAGCCATCAGGAAGC
TGTAGAAATAATAGATAAGCAATCTGACAAAAAGGCTAAACTTTTGGGTCAGAACAAGGATGGTAAAGATATATACGTAT
GTAAGGGCAGGTACGGCTTTTACCTAAAGTGTGACAAGACAAGTGTAGCGCTCAAGGGTAGATCTGAAGACATTACGCTA
GAGGATGCTATATTGTTACTGAGTCAGAAACAGTAA

Upstream 100 bases:

>100_bases
AAGGCGCATTGATGCTTATGATATCAAAACACTTTTCAAGACCCCCTTTTTATAATATTAGGTTCCTAGCTTCAAAGGGA
GCGTTGTTTGTAGGTTTGAG

Downstream 100 bases:

>100_bases
AAGAAGAGCTAATTGGTATAAAGCTAGGGTAGCCTGGGTTGTAATGTGTCGATCGCATTTTTAAGCGCTCTCTCCTGCCT
TCAGCCTGCTGCACAACTTT

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 811; Mature: 810

Protein sequence:

>811_residues
MGVVIVESPSKAKTISKYLGKKFNVIASFGHIRDFPAKSGSVDPSNDFKMIYEIIPKSEKHLDKIVKTVSAEKEAIYLAT
DPDREGEAIAWHIVEVLKEKCAIAKDVTVNRMVFNEVTKRAVTAAVDNCRPINMDLVRAQQTRRVLDYLVGFTLSPLLWR
KLPGSRSAGRVQSVALRLLCEREYEVEQFKTQEYWDVGVDLQNQGGEKFEAVLKCYAGEKLEKFSIGTEEQAKEYAETVK
NQSYTVESVESKKTKRNPYPPFITSSLQQEASTKLGFSAKNTMMIAQKLYEGIDIGGEIVGLITYMRTDGFYISEDAVNY
IRDTIKMQFGEKYLSKSVRNYTKKVKNAQEAHEAIRPTDITMLPEQLSKYLTDEQLKLYTLIWKRTLATQMESAVIDQVA
VDIKSSCGKISLHATGSTLSFDGYYRVYGSEDSDKRRMLPQLREGESCELLEVHPNQHFTQPPARYSEASLVKKMEEIGI
GRPSTYATIISVLQERGYTQLEQKRFIPSERGRIVNAFLTNFFRKYVEYDFTANLEEELDLISNGKMVWKEVLSQFWDKF
ISDVSSVKTIEASEILKAITRDLENYAFSSVDGESISRVCPQCGSGELMLNIGKTSAFLGCNRYPECRYTRGIGGDGSAS
PADDASRILGIDEETQEEIFLKRGPYGEYLQLGNSTKRVSLTKSAGEITVDLAKKMLSLPLVLGCYPDTDKEIKLGIGRF
GPYLFYNGVYFSIKDREDYINISHQEAVEIIDKQSDKKAKLLGQNKDGKDIYVCKGRYGFYLKCDKTSVALKGRSEDITL
EDAILLLSQKQ

Sequences:

>Translated_811_residues
MGVVIVESPSKAKTISKYLGKKFNVIASFGHIRDFPAKSGSVDPSNDFKMIYEIIPKSEKHLDKIVKTVSAEKEAIYLAT
DPDREGEAIAWHIVEVLKEKCAIAKDVTVNRMVFNEVTKRAVTAAVDNCRPINMDLVRAQQTRRVLDYLVGFTLSPLLWR
KLPGSRSAGRVQSVALRLLCEREYEVEQFKTQEYWDVGVDLQNQGGEKFEAVLKCYAGEKLEKFSIGTEEQAKEYAETVK
NQSYTVESVESKKTKRNPYPPFITSSLQQEASTKLGFSAKNTMMIAQKLYEGIDIGGEIVGLITYMRTDGFYISEDAVNY
IRDTIKMQFGEKYLSKSVRNYTKKVKNAQEAHEAIRPTDITMLPEQLSKYLTDEQLKLYTLIWKRTLATQMESAVIDQVA
VDIKSSCGKISLHATGSTLSFDGYYRVYGSEDSDKRRMLPQLREGESCELLEVHPNQHFTQPPARYSEASLVKKMEEIGI
GRPSTYATIISVLQERGYTQLEQKRFIPSERGRIVNAFLTNFFRKYVEYDFTANLEEELDLISNGKMVWKEVLSQFWDKF
ISDVSSVKTIEASEILKAITRDLENYAFSSVDGESISRVCPQCGSGELMLNIGKTSAFLGCNRYPECRYTRGIGGDGSAS
PADDASRILGIDEETQEEIFLKRGPYGEYLQLGNSTKRVSLTKSAGEITVDLAKKMLSLPLVLGCYPDTDKEIKLGIGRF
GPYLFYNGVYFSIKDREDYINISHQEAVEIIDKQSDKKAKLLGQNKDGKDIYVCKGRYGFYLKCDKTSVALKGRSEDITL
EDAILLLSQKQ
>Mature_810_residues
GVVIVESPSKAKTISKYLGKKFNVIASFGHIRDFPAKSGSVDPSNDFKMIYEIIPKSEKHLDKIVKTVSAEKEAIYLATD
PDREGEAIAWHIVEVLKEKCAIAKDVTVNRMVFNEVTKRAVTAAVDNCRPINMDLVRAQQTRRVLDYLVGFTLSPLLWRK
LPGSRSAGRVQSVALRLLCEREYEVEQFKTQEYWDVGVDLQNQGGEKFEAVLKCYAGEKLEKFSIGTEEQAKEYAETVKN
QSYTVESVESKKTKRNPYPPFITSSLQQEASTKLGFSAKNTMMIAQKLYEGIDIGGEIVGLITYMRTDGFYISEDAVNYI
RDTIKMQFGEKYLSKSVRNYTKKVKNAQEAHEAIRPTDITMLPEQLSKYLTDEQLKLYTLIWKRTLATQMESAVIDQVAV
DIKSSCGKISLHATGSTLSFDGYYRVYGSEDSDKRRMLPQLREGESCELLEVHPNQHFTQPPARYSEASLVKKMEEIGIG
RPSTYATIISVLQERGYTQLEQKRFIPSERGRIVNAFLTNFFRKYVEYDFTANLEEELDLISNGKMVWKEVLSQFWDKFI
SDVSSVKTIEASEILKAITRDLENYAFSSVDGESISRVCPQCGSGELMLNIGKTSAFLGCNRYPECRYTRGIGGDGSASP
ADDASRILGIDEETQEEIFLKRGPYGEYLQLGNSTKRVSLTKSAGEITVDLAKKMLSLPLVLGCYPDTDKEIKLGIGRFG
PYLFYNGVYFSIKDREDYINISHQEAVEIIDKQSDKKAKLLGQNKDGKDIYVCKGRYGFYLKCDKTSVALKGRSEDITLE
DAILLLSQKQ

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=606, Percent_Identity=26.8976897689769, Blast_Score=119, Evalue=1e-26,
Organism=Homo sapiens, GI4507635, Length=610, Percent_Identity=21.3114754098361, Blast_Score=95, Evalue=2e-19,
Organism=Escherichia coli, GI1787529, Length=654, Percent_Identity=41.131498470948, Blast_Score=469, Evalue=1e-133,
Organism=Escherichia coli, GI1788061, Length=539, Percent_Identity=26.1595547309833, Blast_Score=131, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI32563869, Length=567, Percent_Identity=23.6331569664903, Blast_Score=117, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17555378, Length=530, Percent_Identity=26.0377358490566, Blast_Score=114, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323263, Length=499, Percent_Identity=22.2444889779559, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24585251, Length=584, Percent_Identity=25, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 91614; Mature: 91482

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVVIVESPSKAKTISKYLGKKFNVIASFGHIRDFPAKSGSVDPSNDFKMIYEIIPKSEK
CCEEEECCCCHHHHHHHHHCCHHHHHHHHCHHHCCCCCCCCCCCCCHHHHHHHHCCCCHH
HLDKIVKTVSAEKEAIYLATDPDREGEAIAWHIVEVLKEKCAIAKDVTVNRMVFNEVTKR
HHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
AVTAAVDNCRPINMDLVRAQQTRRVLDYLVGFTLSPLLWRKLPGSRSAGRVQSVALRLLC
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
EREYEVEQFKTQEYWDVGVDLQNQGGEKFEAVLKCYAGEKLEKFSIGTEEQAKEYAETVK
HCCHHHHHHCCHHHHCCCCEECCCCCHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHC
NQSYTVESVESKKTKRNPYPPFITSSLQQEASTKLGFSAKNTMMIAQKLYEGIDIGGEIV
CCCEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHH
GLITYMRTDGFYISEDAVNYIRDTIKMQFGEKYLSKSVRNYTKKVKNAQEAHEAIRPTDI
HHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TMLPEQLSKYLTDEQLKLYTLIWKRTLATQMESAVIDQVAVDIKSSCGKISLHATGSTLS
EECHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEE
FDGYYRVYGSEDSDKRRMLPQLREGESCELLEVHPNQHFTQPPARYSEASLVKKMEEIGI
ECCEEEEECCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHCC
GRPSTYATIISVLQERGYTQLEQKRFIPSERGRIVNAFLTNFFRKYVEYDFTANLEEELD
CCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHH
LISNGKMVWKEVLSQFWDKFISDVSSVKTIEASEILKAITRDLENYAFSSVDGESISRVC
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHC
PQCGSGELMLNIGKTSAFLGCNRYPECRYTRGIGGDGSASPADDASRILGIDEETQEEIF
CCCCCCCEEEEECCCCEECCCCCCCCCCEECCCCCCCCCCCCCCHHHEECCCCHHHHHHH
LKRGPYGEYLQLGNSTKRVSLTKSAGEITVDLAKKMLSLPLVLGCYPDTDKEIKLGIGRF
HHCCCCHHHHHCCCCCCEEEEECCCCCEEHHHHHHHHHCCEEEECCCCCCCCEEEECCHH
GPYLFYNGVYFSIKDREDYINISHQEAVEIIDKQSDKKAKLLGQNKDGKDIYVCKGRYGF
CCEEEECCEEEEEECCCCCCCCCHHHHHHHHHCCCCCHHHHCCCCCCCCEEEEEECCCEE
YLKCDKTSVALKGRSEDITLEDAILLLSQKQ
EEEECCCEEEECCCCCCCCHHHHHHEEECCC
>Mature Secondary Structure 
GVVIVESPSKAKTISKYLGKKFNVIASFGHIRDFPAKSGSVDPSNDFKMIYEIIPKSEK
CEEEECCCCHHHHHHHHHCCHHHHHHHHCHHHCCCCCCCCCCCCCHHHHHHHHCCCCHH
HLDKIVKTVSAEKEAIYLATDPDREGEAIAWHIVEVLKEKCAIAKDVTVNRMVFNEVTKR
HHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
AVTAAVDNCRPINMDLVRAQQTRRVLDYLVGFTLSPLLWRKLPGSRSAGRVQSVALRLLC
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
EREYEVEQFKTQEYWDVGVDLQNQGGEKFEAVLKCYAGEKLEKFSIGTEEQAKEYAETVK
HCCHHHHHHCCHHHHCCCCEECCCCCHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHC
NQSYTVESVESKKTKRNPYPPFITSSLQQEASTKLGFSAKNTMMIAQKLYEGIDIGGEIV
CCCEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHH
GLITYMRTDGFYISEDAVNYIRDTIKMQFGEKYLSKSVRNYTKKVKNAQEAHEAIRPTDI
HHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TMLPEQLSKYLTDEQLKLYTLIWKRTLATQMESAVIDQVAVDIKSSCGKISLHATGSTLS
EECHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEE
FDGYYRVYGSEDSDKRRMLPQLREGESCELLEVHPNQHFTQPPARYSEASLVKKMEEIGI
ECCEEEEECCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHCC
GRPSTYATIISVLQERGYTQLEQKRFIPSERGRIVNAFLTNFFRKYVEYDFTANLEEELD
CCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHH
LISNGKMVWKEVLSQFWDKFISDVSSVKTIEASEILKAITRDLENYAFSSVDGESISRVC
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHC
PQCGSGELMLNIGKTSAFLGCNRYPECRYTRGIGGDGSASPADDASRILGIDEETQEEIF
CCCCCCCEEEEECCCCEECCCCCCCCCCEECCCCCCCCCCCCCCHHHEECCCCHHHHHHH
LKRGPYGEYLQLGNSTKRVSLTKSAGEITVDLAKKMLSLPLVLGCYPDTDKEIKLGIGRF
HHCCCCHHHHHCCCCCCEEEEECCCCCEEHHHHHHHHHCCEEEECCCCCCCCEEEECCHH
GPYLFYNGVYFSIKDREDYINISHQEAVEIIDKQSDKKAKLLGQNKDGKDIYVCKGRYGF
CCEEEECCEEEEEECCCCCCCCCHHHHHHHHHCCCCCHHHHCCCCCCCCEEEEEECCCEE
YLKCDKTSVALKGRSEDITLEDAILLLSQKQ
EEEECCCEEEECCCCCCCCHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]