Click here to switch to the map view.

The map label for this gene is purK [H]

Identifier: 88607337

GI number: 88607337

Start: 1181775

End: 1182851

Strand: Reverse

Name: purK [H]

Synonym: APH_1119

Alternate gene names: 88607337

Gene position: 1182851-1181775 (Counterclockwise)

Preceding gene: 88607850

Following gene: 88606813

Centisome position: 80.4

GC content: 42.53

Gene sequence:

>1077_bases
ATGCGAACATCTGTCTCTCATAATATGCTATCCGCCACCAGAGTCGGTATTATAGGAGGTGGTCAGCTAGGAAAAATGCT
TTCTCTATCCTCTTCTAAGTTAGGCTATCAAACCAGTATCTTTGCAGAAAGCGCAATTGATCCAGCTGTTTTCGTCACGA
ACTCTTCAGTAATAGGCAGTTTCAGTGATACAGAGAAATTGCGGACCTTTGCTTCAATGGTTGACGTAGCAGTTATAGAA
TTTGAAAATATTCCAGTATCAGCGGTGCATACACTTCAAGAAACTATCCCTGTGTATCCTGGAGTTAAGGCGCTGCATGT
TGCACAGAACAGAATACGTGAGAAAACTCACATACAGTCACTAGGTATTGCTGTAGCTGATTTTGCCGTTATACATAGTC
ATACAGATTTGCATAAGGCCGTGCAAGATGTTGGCGTGCCATCTGTGCTAAAAACTGCTGAATCTGGATATGATGGAAAG
GGGCAATACGTCCTACGCAGCACTGAAGATGTGGGGTCACTAGCCCATTTACCTTTGGAGCAGGGATACATATTGGAAAG
ATTGATCGAGATAGAAAAAGAGATATCTATAATCATAGCTGTCGATCGCAACGCTTCTCATACGTTTTTTCCTGTAGCAA
AGAACACACACGTAGATGGCGTACTGAGTGAATCAATTGTTCCTGCTGGTATATCGACAGACCTCCAGAAACAAGCTCAA
GAGATCGCTTATGCTATAGCAACGTCATTAGAAATGGTGGGCATACTTGCAGTTGAATTCTTCATCTCGAAAAGCGGTAA
GTTGCTAGTGAATGAAATTGCTCCACGTCCACATAATTCAGGTCACTGGAGCCAGGATGCGTGTAATGTTAGCCAATTTG
AACAGCTGATACGTATTGCTTGTTGGCTTCCCATGCGTGTTGTACACCTACTAACACCGTGCGTTATGCGAAATGTTTTT
GGAGATAACATCATTGATGAGGAAATTCACCAAGACCATCGCAATAGCATCTCTCTGTATGGGAAGCAGCCTCGCGCTAA
AAGAAAGATGGGGCATATAAATTCACTGTTATATTAA

Upstream 100 bases:

>100_bases
CCTAAGCAAAGTAATCCACTTTCTTAACATTTGCTGATACTTCACTTATGTCTTATAATCCATTATAGTTGTTTTTCTTC
GCTACATCAATTTCTATAAC

Downstream 100 bases:

>100_bases
GGAACTGTCTTCGTTTTTAAGCACTTGTCTGTGTGCTAAAGACATTGTTGCGAGTCTGCAGCGTGTACTGTAAAGGTGTA
TATCGAAAGATGCACGCTAG

Product: phosphoribosylaminoimidazole carboxylase, ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MRTSVSHNMLSATRVGIIGGGQLGKMLSLSSSKLGYQTSIFAESAIDPAVFVTNSSVIGSFSDTEKLRTFASMVDVAVIE
FENIPVSAVHTLQETIPVYPGVKALHVAQNRIREKTHIQSLGIAVADFAVIHSHTDLHKAVQDVGVPSVLKTAESGYDGK
GQYVLRSTEDVGSLAHLPLEQGYILERLIEIEKEISIIIAVDRNASHTFFPVAKNTHVDGVLSESIVPAGISTDLQKQAQ
EIAYAIATSLEMVGILAVEFFISKSGKLLVNEIAPRPHNSGHWSQDACNVSQFEQLIRIACWLPMRVVHLLTPCVMRNVF
GDNIIDEEIHQDHRNSISLYGKQPRAKRKMGHINSLLY

Sequences:

>Translated_358_residues
MRTSVSHNMLSATRVGIIGGGQLGKMLSLSSSKLGYQTSIFAESAIDPAVFVTNSSVIGSFSDTEKLRTFASMVDVAVIE
FENIPVSAVHTLQETIPVYPGVKALHVAQNRIREKTHIQSLGIAVADFAVIHSHTDLHKAVQDVGVPSVLKTAESGYDGK
GQYVLRSTEDVGSLAHLPLEQGYILERLIEIEKEISIIIAVDRNASHTFFPVAKNTHVDGVLSESIVPAGISTDLQKQAQ
EIAYAIATSLEMVGILAVEFFISKSGKLLVNEIAPRPHNSGHWSQDACNVSQFEQLIRIACWLPMRVVHLLTPCVMRNVF
GDNIIDEEIHQDHRNSISLYGKQPRAKRKMGHINSLLY
>Mature_358_residues
MRTSVSHNMLSATRVGIIGGGQLGKMLSLSSSKLGYQTSIFAESAIDPAVFVTNSSVIGSFSDTEKLRTFASMVDVAVIE
FENIPVSAVHTLQETIPVYPGVKALHVAQNRIREKTHIQSLGIAVADFAVIHSHTDLHKAVQDVGVPSVLKTAESGYDGK
GQYVLRSTEDVGSLAHLPLEQGYILERLIEIEKEISIIIAVDRNASHTFFPVAKNTHVDGVLSESIVPAGISTDLQKQAQ
EIAYAIATSLEMVGILAVEFFISKSGKLLVNEIAPRPHNSGHWSQDACNVSQFEQLIRIACWLPMRVVHLLTPCVMRNVF
GDNIIDEEIHQDHRNSISLYGKQPRAKRKMGHINSLLY

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=343, Percent_Identity=29.4460641399417, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1788155, Length=314, Percent_Identity=26.7515923566879, Blast_Score=98, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6324702, Length=365, Percent_Identity=33.972602739726, Blast_Score=194, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006115
- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp; PF03446 NAD_binding_2 [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 39211; Mature: 39211

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSVSHNMLSATRVGIIGGGQLGKMLSLSSSKLGYQTSIFAESAIDPAVFVTNSSVIGS
CCCCCCHHHHHHHHEEEEECCHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCC
FSDTEKLRTFASMVDVAVIEFENIPVSAVHTLQETIPVYPGVKALHVAQNRIREKTHIQS
CCHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LGIAVADFAVIHSHTDLHKAVQDVGVPSVLKTAESGYDGKGQYVLRSTEDVGSLAHLPLE
HHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEECCHHHHHHHCCCCC
QGYILERLIEIEKEISIIIAVDRNASHTFFPVAKNTHVDGVLSESIVPAGISTDLQKQAQ
CCHHHHHHHHHHCCEEEEEEECCCCCCEEEECCCCCCCCCHHHCCCCCCCCCHHHHHHHH
EIAYAIATSLEMVGILAVEFFISKSGKLLVNEIAPRPHNSGHWSQDACNVSQFEQLIRIA
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHH
CWLPMRVVHLLTPCVMRNVFGDNIIDEEIHQDHRNSISLYGKQPRAKRKMGHINSLLY
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRTSVSHNMLSATRVGIIGGGQLGKMLSLSSSKLGYQTSIFAESAIDPAVFVTNSSVIGS
CCCCCCHHHHHHHHEEEEECCHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCC
FSDTEKLRTFASMVDVAVIEFENIPVSAVHTLQETIPVYPGVKALHVAQNRIREKTHIQS
CCHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LGIAVADFAVIHSHTDLHKAVQDVGVPSVLKTAESGYDGKGQYVLRSTEDVGSLAHLPLE
HHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEECCHHHHHHHCCCCC
QGYILERLIEIEKEISIIIAVDRNASHTFFPVAKNTHVDGVLSESIVPAGISTDLQKQAQ
CCHHHHHHHHHHCCEEEEEEECCCCCCEEEECCCCCCCCCHHHCCCCCCCCCHHHHHHHH
EIAYAIATSLEMVGILAVEFFISKSGKLLVNEIAPRPHNSGHWSQDACNVSQFEQLIRIA
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHH
CWLPMRVVHLLTPCVMRNVFGDNIIDEEIHQDHRNSISLYGKQPRAKRKMGHINSLLY
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]