Click here to switch to the map view.

The map label for this gene is rpoH [H]

Identifier: 88607306

GI number: 88607306

Start: 805744

End: 806613

Strand: Reverse

Name: rpoH [H]

Synonym: APH_0759

Alternate gene names: 88607306

Gene position: 806613-805744 (Counterclockwise)

Preceding gene: 88607455

Following gene: 88606720

Centisome position: 54.82

GC content: 41.49

Gene sequence:

>870_bases
ATGCTTACCGCATCTGGGTTATCGATGGGAGCAGATGATTTACTTGCATATATGGAGCAAGTAAGGGCTTTTCCTATGCT
AACAGAGAGAGAAGAGAAGGAATGTGCAGAGAATTGGCATAAAAATAGATCGGTAGCTGACGCTCATAAGTTGGTAACTA
GCCATCTGAGGTTAGTGGTTAAAGTAGCAATGGGTTTTAGGAGTTATGGCCTTCCTCTCATGGAGTTAATAATGGAAGGA
AACATAGGTCTGATGCAAGCAGTGAAGAAGTTCAATCCCGAATTGGGCTTCAGGTTGTCTACATATGCAATATGGTGGAT
AAAAGCGTCTATAAAAGACTATATACTGCGTTCCTGGTCGTGTATGCGTATAGGGACCACGCAGGCTCAGAAGAAGTTGT
TTTTTAGTTTGAGGAAGATTAAAAGGAGAATATTAGGCTGTAGTGCTTCGGCCAGTCAGAGTGATATACGTGCTATAGCG
GAGGAATGCGGTACTTCTGAAAAGGAAGTAGAGAGCATGGATAGGTTTTTCTCCAATAGGGACATATCACTCAGTGACAA
GGTCTGCTGTGAAAGTGGTACTGAGCTACAAGATCTTATACCATGTTCAAGCCCCAATCAGGAGGTTATGTACCTATCCC
ATGAGGAGAGTGTGATGAAAGATCAGATGGTAAAGGAGGCTTTATCTACGCTGGATGAAAGACATAGAGATATTTTTTCG
CGAAGAAAGTTAAAGGAACATCCAGACACTCTAGAAACGTTAAGTGAAGAATATGGAGTCTCAAAGGAAAGAATCAGGCA
GATTGAGCTTCAATCTTTCAGTAAGGTAAGGTCTTTTGTAAACGCTAATAGATACAAGATATGCTGCTAG

Upstream 100 bases:

>100_bases
AAAATTATTAAAGATGTAGCAAAGTCTTGAAAATTGCTCATACCATACCTATACTAGAGTGCGTACATAGTGCAATTAAT
AAACAGAGAGGCGTTGGTAT

Downstream 100 bases:

>100_bases
TTTATAGCGTCTACTGTGTTGGATTAGATTAAAGTCCGGTTTGAGGCTGATTTTCTGATAACATAAGTATGAGTAGATGT
GCGGTAGTGTGTAAAGAGGT

Product: RNA polymerase sigma-32 factor

Products: diphosphate; RNAn+1

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MLTASGLSMGADDLLAYMEQVRAFPMLTEREEKECAENWHKNRSVADAHKLVTSHLRLVVKVAMGFRSYGLPLMELIMEG
NIGLMQAVKKFNPELGFRLSTYAIWWIKASIKDYILRSWSCMRIGTTQAQKKLFFSLRKIKRRILGCSASASQSDIRAIA
EECGTSEKEVESMDRFFSNRDISLSDKVCCESGTELQDLIPCSSPNQEVMYLSHEESVMKDQMVKEALSTLDERHRDIFS
RRKLKEHPDTLETLSEEYGVSKERIRQIELQSFSKVRSFVNANRYKICC

Sequences:

>Translated_289_residues
MLTASGLSMGADDLLAYMEQVRAFPMLTEREEKECAENWHKNRSVADAHKLVTSHLRLVVKVAMGFRSYGLPLMELIMEG
NIGLMQAVKKFNPELGFRLSTYAIWWIKASIKDYILRSWSCMRIGTTQAQKKLFFSLRKIKRRILGCSASASQSDIRAIA
EECGTSEKEVESMDRFFSNRDISLSDKVCCESGTELQDLIPCSSPNQEVMYLSHEESVMKDQMVKEALSTLDERHRDIFS
RRKLKEHPDTLETLSEEYGVSKERIRQIELQSFSKVRSFVNANRYKICC
>Mature_289_residues
MLTASGLSMGADDLLAYMEQVRAFPMLTEREEKECAENWHKNRSVADAHKLVTSHLRLVVKVAMGFRSYGLPLMELIMEG
NIGLMQAVKKFNPELGFRLSTYAIWWIKASIKDYILRSWSCMRIGTTQAQKKLFFSLRKIKRRILGCSASASQSDIRAIA
EECGTSEKEVESMDRFFSNRDISLSDKVCCESGTELQDLIPCSSPNQEVMYLSHEESVMKDQMVKEALSTLDERHRDIFS
RRKLKEHPDTLETLSEEYGVSKERIRQIELQSFSKVRSFVNANRYKICC

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=283, Percent_Identity=37.8091872791519, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI1789098, Length=293, Percent_Identity=31.0580204778157, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1789448, Length=237, Percent_Identity=27.8481012658228, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: 2.7.7.6

Molecular weight: Translated: 33223; Mature: 33223

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
7.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTASGLSMGADDLLAYMEQVRAFPMLTEREEKECAENWHKNRSVADAHKLVTSHLRLVV
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
KVAMGFRSYGLPLMELIMEGNIGLMQAVKKFNPELGFRLSTYAIWWIKASIKDYILRSWS
HHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHCCCCCCEEHHHEEEHHHHHHHHHHHHCCC
CMRIGTTQAQKKLFFSLRKIKRRILGCSASASQSDIRAIAEECGTSEKEVESMDRFFSNR
HHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCC
DISLSDKVCCESGTELQDLIPCSSPNQEVMYLSHEESVMKDQMVKEALSTLDERHRDIFS
CCCCCHHHHHCCCCCHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
RRKLKEHPDTLETLSEEYGVSKERIRQIELQSFSKVRSFVNANRYKICC
HHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEECC
>Mature Secondary Structure
MLTASGLSMGADDLLAYMEQVRAFPMLTEREEKECAENWHKNRSVADAHKLVTSHLRLVV
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
KVAMGFRSYGLPLMELIMEGNIGLMQAVKKFNPELGFRLSTYAIWWIKASIKDYILRSWS
HHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHCCCCCCEEHHHEEEHHHHHHHHHHHHCCC
CMRIGTTQAQKKLFFSLRKIKRRILGCSASASQSDIRAIAEECGTSEKEVESMDRFFSNR
HHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCC
DISLSDKVCCESGTELQDLIPCSSPNQEVMYLSHEESVMKDQMVKEALSTLDERHRDIFS
CCCCCHHHHHCCCCCHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
RRKLKEHPDTLETLSEEYGVSKERIRQIELQSFSKVRSFVNANRYKICC
HHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nucleoside triphosphate; RNAn

Specific reaction: nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606166 [H]