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The map label for this gene is birA [H]

Identifier: 88607177

GI number: 88607177

Start: 930013

End: 930738

Strand: Reverse

Name: birA [H]

Synonym: APH_0876

Alternate gene names: 88607177

Gene position: 930738-930013 (Counterclockwise)

Preceding gene: 88607142

Following gene: 88607936

Centisome position: 63.26

GC content: 41.46

Gene sequence:

>726_bases
ATGAAGCCATTTGTAATTCATAGGCGTAGCATCAGCCAAAGTACTAACTTAGATGCAATGGAATTAGCGGAGCAAGGTGC
AGATTCAGGTACGGTGATTGTCGCTGATGAGCAAAGTTCAGGTGTTGGTAGGCGCGGCAGACAGTGGTATAGCCCTAAAG
GAAATCTATATTTTAGTATTGTTTTAAAGGAGCATTTGCATGTACCCACGCTTTCATTCGCTTGTGCCTTATCCGTGGGG
ATATCTCTTGAGCAAATTTTTTCCCAACATATAGAAAATTCAAAAATTCCAACTCTAAAATACAAGTGGCCAAACGACGT
TTTTGTGGACAACAAGAAAATTTCTGGCGTACTGCTGCAGAGCAAAGTGCAGGGCCATTCTATACCCTGGATAGTATGTG
GCGTAGGTGTGAATATATTGCATCATCCAGAATATGCGACCTCGATAAGCAGCTATATTCCAGATCTCAAATTGACCAAT
ATAGAGCTGTTGGATGTGGTATTGGAAAATCTAGCTAAACTTCTCGATATATATTCTGCGTCAGGATTTGCTCCGCTAAG
AAGTTTGTGGCTAAAGAAAGCGCATGCGCTAAACTCACACGTCTGCATAACAACAAGCGATGGAATCACCCATGAAGGTA
CGTTTACCGATATTGGTCTTGACGGTTCTATAGTATTGAAATCTGGTGAAGATACCTTGAAGCTAGACTATGGCTCGATG
TTATAA

Upstream 100 bases:

>100_bases
TTTAGCAACAGTGTGACATAATAGTCAACTTTTCGCTGTTCTATTATTGCAGGCAACATAAGGGGAGAGTAAATTTACAG
CGAGATAATAGAGGCAAGTA

Downstream 100 bases:

>100_bases
TAGCCATAACGGGAACTATACTGCTATAAGCTGCGGGTTTGTGGAGAATGCATGATAAAGACAAGGGTTTGTTCTAAAAT
CTCATGAGTACTACTGAGGC

Product: biotin--acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin--protein ligase [H]

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MKPFVIHRRSISQSTNLDAMELAEQGADSGTVIVADEQSSGVGRRGRQWYSPKGNLYFSIVLKEHLHVPTLSFACALSVG
ISLEQIFSQHIENSKIPTLKYKWPNDVFVDNKKISGVLLQSKVQGHSIPWIVCGVGVNILHHPEYATSISSYIPDLKLTN
IELLDVVLENLAKLLDIYSASGFAPLRSLWLKKAHALNSHVCITTSDGITHEGTFTDIGLDGSIVLKSGEDTLKLDYGSM
L

Sequences:

>Translated_241_residues
MKPFVIHRRSISQSTNLDAMELAEQGADSGTVIVADEQSSGVGRRGRQWYSPKGNLYFSIVLKEHLHVPTLSFACALSVG
ISLEQIFSQHIENSKIPTLKYKWPNDVFVDNKKISGVLLQSKVQGHSIPWIVCGVGVNILHHPEYATSISSYIPDLKLTN
IELLDVVLENLAKLLDIYSASGFAPLRSLWLKKAHALNSHVCITTSDGITHEGTFTDIGLDGSIVLKSGEDTLKLDYGSM
L
>Mature_241_residues
MKPFVIHRRSISQSTNLDAMELAEQGADSGTVIVADEQSSGVGRRGRQWYSPKGNLYFSIVLKEHLHVPTLSFACALSVG
ISLEQIFSQHIENSKIPTLKYKWPNDVFVDNKKISGVLLQSKVQGHSIPWIVCGVGVNILHHPEYATSISSYIPDLKLTN
IELLDVVLENLAKLLDIYSASGFAPLRSLWLKKAHALNSHVCITTSDGITHEGTFTDIGLDGSIVLKSGEDTLKLDYGSM
L

Specific function: Activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins [H]

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=185, Percent_Identity=27.027027027027, Blast_Score=84, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR003142
- InterPro:   IPR004143 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 26486; Mature: 26486

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPFVIHRRSISQSTNLDAMELAEQGADSGTVIVADEQSSGVGRRGRQWYSPKGNLYFSI
CCCEEEEECCCCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEE
VLKEHLHVPTLSFACALSVGISLEQIFSQHIENSKIPTLKYKWPNDVFVDNKKISGVLLQ
EEECCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCEEECCCCCCEEEEE
SKVQGHSIPWIVCGVGVNILHHPEYATSISSYIPDLKLTNIELLDVVLENLAKLLDIYSA
ECCCCCCCCEEEECCCCEEEECCHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHCC
SGFAPLRSLWLKKAHALNSHVCITTSDGITHEGTFTDIGLDGSIVLKSGEDTLKLDYGSM
CCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEECCCCEEEEECCCCEEEECCCCC
L
C
>Mature Secondary Structure
MKPFVIHRRSISQSTNLDAMELAEQGADSGTVIVADEQSSGVGRRGRQWYSPKGNLYFSI
CCCEEEEECCCCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEE
VLKEHLHVPTLSFACALSVGISLEQIFSQHIENSKIPTLKYKWPNDVFVDNKKISGVLLQ
EEECCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCEEECCCCCCEEEEE
SKVQGHSIPWIVCGVGVNILHHPEYATSISSYIPDLKLTNIELLDVVLENLAKLLDIYSA
ECCCCCCCCEEEECCCCEEEECCHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHCC
SGFAPLRSLWLKKAHALNSHVCITTSDGITHEGTFTDIGLDGSIVLKSGEDTLKLDYGSM
CCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEECCCCEEEEECCCCEEEECCCCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]