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The map label for this gene is mraW

Identifier: 88606985

GI number: 88606985

Start: 711865

End: 712752

Strand: Direct

Name: mraW

Synonym: APH_0690

Alternate gene names: 88606985

Gene position: 711865-712752 (Clockwise)

Preceding gene: 88607527

Following gene: 88607393

Centisome position: 48.38

GC content: 40.99

Gene sequence:

>888_bases
ATGGATTCTCATAGACCTGTACTACTAAACGAAATGCTAGAGCTTCTTTCTCCTAAAGACGGTGCAGTATATGTTGACGC
AACGTTTGGTGGAGGCGGCTATAGTAGGGGAATATTGGAACGGGCTAAATGTACTGTACTAGCTATTGATCAAGATCCTA
TTGCTTCAGAATTCTATCAATCTTTACATTCGCAATTCCCTGGACGGGTGCACTTTTTTCTCAGCAAGTTCAGTAGGCTT
CGAGAGGCGTTACAGCATTTTGATCACAATCAAGTAGATGGTGTTGTATTTGATGTTGGAGTATCAAGTATGCAGCTGAG
TGATCCAGACAGGGGATTCTCATTTATGAAGGAAGGGCCACTTGATATGAGAATGGATATGTTGCGCTCTCAATCCAAAA
GCGCCGCGACTTTTGTAAATTCTTTATCAGAAAAAGACATGGCAGATGTAATCTTCTATTACGGAGGAGAGAGGCTTTCA
AGACGCGTTACCAGAGCTATAATCAGCGCTAGAGAGAGTAATAATAAGATACGTAACACGAGTGACTTGGCAAAAATTAT
CAGGTCTGTTGTGCCCCGCAGTAAGGCAAATCCAATAGATCCGGCAACAAGGACCTTTCAGGCTATCAGAATATGGGTAA
ATGATGAGCTAGAAGAGCTAAAAGCAGGTCTAGAAGCTGCTTCTCAAATTGTAGGAGTTGGAGGCAAGATTATTGTTACT
TCATTTCATTCACTCGAGGATAGGATAGTAAAACATAAGTTCAACGCTCTACGAGAGAAGGGATTTCAACTTATCAATAA
AAAAGCTATAAAGCCCACTGAAAAAGAAATTAGCGAAAATACTAGGTCGCGATCAGCAAAATTGCGTGGCATAGTAAAAG
AGGAGTAA

Upstream 100 bases:

>100_bases
TTTGTTTTTATACTGCTATGCCCTGTTAGGGTACGAGTACTAAGTATAATAATCTAAGTATTAAGTGGAGAATATCATAG
GATTTTTAAAAGTAGAATTA

Downstream 100 bases:

>100_bases
TCACAGTAGAAAATTACGTTGATTAGTAAGTAATTACGGCGAGTTGCAGGGCTTTTTCTCGGTTATATGAAAAGCAGCGA
TATTCTTTGGTAGATTATTT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MDSHRPVLLNEMLELLSPKDGAVYVDATFGGGGYSRGILERAKCTVLAIDQDPIASEFYQSLHSQFPGRVHFFLSKFSRL
REALQHFDHNQVDGVVFDVGVSSMQLSDPDRGFSFMKEGPLDMRMDMLRSQSKSAATFVNSLSEKDMADVIFYYGGERLS
RRVTRAIISARESNNKIRNTSDLAKIIRSVVPRSKANPIDPATRTFQAIRIWVNDELEELKAGLEAASQIVGVGGKIIVT
SFHSLEDRIVKHKFNALREKGFQLINKKAIKPTEKEISENTRSRSAKLRGIVKEE

Sequences:

>Translated_295_residues
MDSHRPVLLNEMLELLSPKDGAVYVDATFGGGGYSRGILERAKCTVLAIDQDPIASEFYQSLHSQFPGRVHFFLSKFSRL
REALQHFDHNQVDGVVFDVGVSSMQLSDPDRGFSFMKEGPLDMRMDMLRSQSKSAATFVNSLSEKDMADVIFYYGGERLS
RRVTRAIISARESNNKIRNTSDLAKIIRSVVPRSKANPIDPATRTFQAIRIWVNDELEELKAGLEAASQIVGVGGKIIVT
SFHSLEDRIVKHKFNALREKGFQLINKKAIKPTEKEISENTRSRSAKLRGIVKEE
>Mature_295_residues
MDSHRPVLLNEMLELLSPKDGAVYVDATFGGGGYSRGILERAKCTVLAIDQDPIASEFYQSLHSQFPGRVHFFLSKFSRL
REALQHFDHNQVDGVVFDVGVSSMQLSDPDRGFSFMKEGPLDMRMDMLRSQSKSAATFVNSLSEKDMADVIFYYGGERLS
RRVTRAIISARESNNKIRNTSDLAKIIRSVVPRSKANPIDPATRTFQAIRIWVNDELEELKAGLEAASQIVGVGGKIIVT
SFHSLEDRIVKHKFNALREKGFQLINKKAIKPTEKEISENTRSRSAKLRGIVKEE

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=336, Percent_Identity=36.3095238095238, Blast_Score=204, Evalue=9e-53,
Organism=Homo sapiens, GI165377202, Length=188, Percent_Identity=38.8297872340425, Blast_Score=134, Evalue=8e-32,
Organism=Escherichia coli, GI1786270, Length=310, Percent_Identity=40, Blast_Score=198, Evalue=4e-52,
Organism=Drosophila melanogaster, GI62472493, Length=328, Percent_Identity=36.5853658536585, Blast_Score=207, Evalue=8e-54,
Organism=Drosophila melanogaster, GI28571637, Length=328, Percent_Identity=36.5853658536585, Blast_Score=207, Evalue=8e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_ANAPZ (Q2GK29)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_505272.1
- ProteinModelPortal:   Q2GK29
- SMR:   Q2GK29
- STRING:   Q2GK29
- GeneID:   3931335
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0690
- NMPDR:   fig|212042.5.peg.671
- TIGR:   APH_0690
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- BioCyc:   APHA212042:APH_0690-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 33135; Mature: 33135

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: BINDING 50-50 BINDING 77-77 BINDING 98-98 BINDING 105-105

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSHRPVLLNEMLELLSPKDGAVYVDATFGGGGYSRGILERAKCTVLAIDQDPIASEFYQ
CCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHCCEEEEEECCCCCHHHHHH
SLHSQFPGRVHFFLSKFSRLREALQHFDHNQVDGVVFDVGVSSMQLSDPDRGFSFMKEGP
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEECCCCCCCCHHHHCCC
LDMRMDMLRSQSKSAATFVNSLSEKDMADVIFYYGGERLSRRVTRAIISARESNNKIRNT
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCH
SDLAKIIRSVVPRSKANPIDPATRTFQAIRIWVNDELEELKAGLEAASQIVGVGGKIIVT
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHCCCCEEEEE
SFHSLEDRIVKHKFNALREKGFQLINKKAIKPTEKEISENTRSRSAKLRGIVKEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDSHRPVLLNEMLELLSPKDGAVYVDATFGGGGYSRGILERAKCTVLAIDQDPIASEFYQ
CCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHCCEEEEEECCCCCHHHHHH
SLHSQFPGRVHFFLSKFSRLREALQHFDHNQVDGVVFDVGVSSMQLSDPDRGFSFMKEGP
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEECCCCCCCCHHHHCCC
LDMRMDMLRSQSKSAATFVNSLSEKDMADVIFYYGGERLSRRVTRAIISARESNNKIRNT
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCH
SDLAKIIRSVVPRSKANPIDPATRTFQAIRIWVNDELEELKAGLEAASQIVGVGGKIIVT
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHCCCCEEEEE
SFHSLEDRIVKHKFNALREKGFQLINKKAIKPTEKEISENTRSRSAKLRGIVKEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA